ORF3a protein [Severe acute respiratory syndrome coronavirus 2]
Protein Classification
List of domain hits
| Name | Accession | Description | Interval | E-value | |||||
| SARS-CoV-like_ORF3a | cd21648 | accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and ... |
5-274 | 0e+00 | |||||
accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and similar proteins from related betacoronavirus; This model represents the accessory protein ORF3a of Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV), SARS-COV-2 (also called 2019 novel coronavirus or 2019-nCoV), and related betacoronaviruses in the Sarbecovirus subgenus (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and replicase/protease polyproteins (ORF1ab); all are required to produce a structurally complete viral particle. In addition, CoV genomes also contain ORFs coding for accessory proteins that are specific for certain CoV lineages or for a particular CoV. In general, CoV accessory proteins are considered to be dispensable for viral replication; however, several accessory proteins have been shown to exhibit functions in virus-host interactions during CoV infection. SARS-CoV mRNA 3 encodes the distinct proteins ORF3a and ORF3b, which are translated in different reading frames. Accessory protein ORF3a, also called protein 3a and protein X1, is the largest ORF protein in SARS-CoV. It is also called accessory protein 3 or protein 3 in some bat coronaviruses. SARS-CoV ORF3a promotes membrane rearrangement and cell death; it induces vesicle formation and is necessary for SARS-CoV-induced Golgi fragmentation. It has also been found to activate NF-kappaB and the NLRP3 inflammasome by promoting TNF receptor-associated factor 3 (TRAF3)-dependent ubiquitination of p105 and ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain). The cytoplasmic domain of SARS-CoV ORF3a, composed of amino acids at the C-terminal region, has sequence similarity to a calcium pump present in Plasmodium falciparum and has been shown to bind calcium in vitro. SARS-CoV-2 3a is able to form ion channels; it is a Class IIIA viroporin, having 3 transmembrane helices per protomer and a lumenal amino terminus and cytosolic carboxyl terminus. It can form dimers, tetramers, and potentially higher order oligomers. It has been shown to form cation channels with modest selectivity for Ca2+ and K+ over Na+. : Pssm-ID: 439223 Cd Length: 269 Bit Score: 527.11 E-value: 0e+00
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| Name | Accession | Description | Interval | E-value | |||||
| SARS-CoV-like_ORF3a | cd21648 | accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and ... |
5-274 | 0e+00 | |||||
accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and similar proteins from related betacoronavirus; This model represents the accessory protein ORF3a of Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV), SARS-COV-2 (also called 2019 novel coronavirus or 2019-nCoV), and related betacoronaviruses in the Sarbecovirus subgenus (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and replicase/protease polyproteins (ORF1ab); all are required to produce a structurally complete viral particle. In addition, CoV genomes also contain ORFs coding for accessory proteins that are specific for certain CoV lineages or for a particular CoV. In general, CoV accessory proteins are considered to be dispensable for viral replication; however, several accessory proteins have been shown to exhibit functions in virus-host interactions during CoV infection. SARS-CoV mRNA 3 encodes the distinct proteins ORF3a and ORF3b, which are translated in different reading frames. Accessory protein ORF3a, also called protein 3a and protein X1, is the largest ORF protein in SARS-CoV. It is also called accessory protein 3 or protein 3 in some bat coronaviruses. SARS-CoV ORF3a promotes membrane rearrangement and cell death; it induces vesicle formation and is necessary for SARS-CoV-induced Golgi fragmentation. It has also been found to activate NF-kappaB and the NLRP3 inflammasome by promoting TNF receptor-associated factor 3 (TRAF3)-dependent ubiquitination of p105 and ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain). The cytoplasmic domain of SARS-CoV ORF3a, composed of amino acids at the C-terminal region, has sequence similarity to a calcium pump present in Plasmodium falciparum and has been shown to bind calcium in vitro. SARS-CoV-2 3a is able to form ion channels; it is a Class IIIA viroporin, having 3 transmembrane helices per protomer and a lumenal amino terminus and cytosolic carboxyl terminus. It can form dimers, tetramers, and potentially higher order oligomers. It has been shown to form cation channels with modest selectivity for Ca2+ and K+ over Na+. Pssm-ID: 439223 Cd Length: 269 Bit Score: 527.11 E-value: 0e+00
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| bCoV_viroporin | pfam11289 | Betacoronavirus viroporin; This entry represents protein 3a encoded by Orf3/3a, also known as ... |
1-274 | 6.49e-158 | |||||
Betacoronavirus viroporin; This entry represents protein 3a encoded by Orf3/3a, also known as X1, which forms homotetrameric potassium, sodium or calcium sensitive ion channels (viroporin) that causes ER-stress in host cells and may modulate virus release. This is a pro-apoptosis-inducing protein that localizes to the endoplasmic reticulum (ER)-Golgi compartment. SARS-CoV causes apoptosis of infected cells through NLRP3 inflammasome activation. Protein 3a also promotes the ubiquitination of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mediated by its interaction with TNF receptor-associated factor 3 (TRAF3). This protein induces NF-kappa B activation and upregulates fibrinogen secretion with high cytokine production. Through the activation of the PERK pathway of unfolded protein response (UPR), this protein causes phosphorylation of eIF2-alpha leading to reduced translation of cellular proteins and as the activation of pro-apoptotic downstream effectors (i.e ATF4, CHOP). Pssm-ID: 463255 Cd Length: 273 Bit Score: 440.24 E-value: 6.49e-158
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| Name | Accession | Description | Interval | E-value | |||||
| SARS-CoV-like_ORF3a | cd21648 | accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and ... |
5-274 | 0e+00 | |||||
accessory protein ORF3a of severe acute respiratory syndrome-associated coronavirus and similar proteins from related betacoronavirus; This model represents the accessory protein ORF3a of Severe Acute Respiratory Syndrome-associated coronavirus (SARS-CoV), SARS-COV-2 (also called 2019 novel coronavirus or 2019-nCoV), and related betacoronaviruses in the Sarbecovirus subgenus (B lineage). There are five essential genes in CoVs that result in the following gene products: Spike (S) protein, Membrane (M) glycoprotein, Nucleocapsid (N), Envelope (E) protein, and replicase/protease polyproteins (ORF1ab); all are required to produce a structurally complete viral particle. In addition, CoV genomes also contain ORFs coding for accessory proteins that are specific for certain CoV lineages or for a particular CoV. In general, CoV accessory proteins are considered to be dispensable for viral replication; however, several accessory proteins have been shown to exhibit functions in virus-host interactions during CoV infection. SARS-CoV mRNA 3 encodes the distinct proteins ORF3a and ORF3b, which are translated in different reading frames. Accessory protein ORF3a, also called protein 3a and protein X1, is the largest ORF protein in SARS-CoV. It is also called accessory protein 3 or protein 3 in some bat coronaviruses. SARS-CoV ORF3a promotes membrane rearrangement and cell death; it induces vesicle formation and is necessary for SARS-CoV-induced Golgi fragmentation. It has also been found to activate NF-kappaB and the NLRP3 inflammasome by promoting TNF receptor-associated factor 3 (TRAF3)-dependent ubiquitination of p105 and ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain). The cytoplasmic domain of SARS-CoV ORF3a, composed of amino acids at the C-terminal region, has sequence similarity to a calcium pump present in Plasmodium falciparum and has been shown to bind calcium in vitro. SARS-CoV-2 3a is able to form ion channels; it is a Class IIIA viroporin, having 3 transmembrane helices per protomer and a lumenal amino terminus and cytosolic carboxyl terminus. It can form dimers, tetramers, and potentially higher order oligomers. It has been shown to form cation channels with modest selectivity for Ca2+ and K+ over Na+. Pssm-ID: 439223 Cd Length: 269 Bit Score: 527.11 E-value: 0e+00
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| bCoV_viroporin | pfam11289 | Betacoronavirus viroporin; This entry represents protein 3a encoded by Orf3/3a, also known as ... |
1-274 | 6.49e-158 | |||||
Betacoronavirus viroporin; This entry represents protein 3a encoded by Orf3/3a, also known as X1, which forms homotetrameric potassium, sodium or calcium sensitive ion channels (viroporin) that causes ER-stress in host cells and may modulate virus release. This is a pro-apoptosis-inducing protein that localizes to the endoplasmic reticulum (ER)-Golgi compartment. SARS-CoV causes apoptosis of infected cells through NLRP3 inflammasome activation. Protein 3a also promotes the ubiquitination of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mediated by its interaction with TNF receptor-associated factor 3 (TRAF3). This protein induces NF-kappa B activation and upregulates fibrinogen secretion with high cytokine production. Through the activation of the PERK pathway of unfolded protein response (UPR), this protein causes phosphorylation of eIF2-alpha leading to reduced translation of cellular proteins and as the activation of pro-apoptotic downstream effectors (i.e ATF4, CHOP). Pssm-ID: 463255 Cd Length: 273 Bit Score: 440.24 E-value: 6.49e-158
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