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Conserved domains on  [gi|578826238|ref|XP_006720360|]
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DDB1- and CUL4-associated factor 5 isoform X1 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 11457048)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
49-250 8.84e-27

WD40 repeat [General function prediction only];


:

Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 113.85  E-value: 8.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAihsrvKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:COG2319  158 LTGHSGAVTSVAFSPDG-KLLASGSDDGTVRLWDLATG-----KLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLW 231

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 129 DVESSETLDVFA-HEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIRESPHGEPfcLANYPSAFHSVMFNPvEPRLLATAN 207
Cdd:COG2319  232 DLATGKLLRTLTgHSGSVRSVAFSP-DGRLLASGSADGTVRLWDLATGELLRT--LTGHSGGVNSVAFSP-DGKLLASGS 307

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 578826238 208 SKEGVGLWDIRKPQsSLLRYGGNLSlqSAMSVRFNSNGTQLLA 250
Cdd:COG2319  308 DDGTVRLWDLATGK-LLRTLTGHTG--AVRSVAFSPDGKTLAS 347
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 362-396 1.42e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


:

Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 39.99  E-value: 1.42e-04
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 578826238   362 MVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:smart00320   6 KTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
49-250 8.84e-27

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 113.85  E-value: 8.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAihsrvKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:COG2319  158 LTGHSGAVTSVAFSPDG-KLLASGSDDGTVRLWDLATG-----KLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLW 231

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 129 DVESSETLDVFA-HEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIRESPHGEPfcLANYPSAFHSVMFNPvEPRLLATAN 207
Cdd:COG2319  232 DLATGKLLRTLTgHSGSVRSVAFSP-DGRLLASGSADGTVRLWDLATGELLRT--LTGHSGGVNSVAFSP-DGKLLASGS 307

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 578826238 208 SKEGVGLWDIRKPQsSLLRYGGNLSlqSAMSVRFNSNGTQLLA 250
Cdd:COG2319  308 DDGTVRLWDLATGK-LLRTLTGHTG--AVRSVAFSPDGKTLAS 347
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 49-396 1.30e-26

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 110.89  E-value: 1.30e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAIHSRvkpiQLKGeHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:cd00200    5 LKGHTGGVTCVAFSPDG-KLLATGSGDGTIKVWDLETGELLR----TLKG-HTGPVRDVAASADGTYLASGSSDKTIRLW 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 129 DVESSETLDVFA-HEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIRESPhgepfCLANYPSAFHSVM---FNPvEPRLLA 204
Cdd:cd00200   79 DLETGECVRTLTgHTSYVSSVAFSP-DGRILSSSSRDKTIKVWDVETGK-----CLTTLRGHTDWVNsvaFSP-DGTFVA 151

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 205 TAnSKEG-VGLWDIRKPQSSLLRYGGNLSLQsamSVRFNSNGTQLLA------LRrrlppvLYDIHSRLPVFQFD-NQGY 276
Cdd:cd00200  152 SS-SQDGtIKLWDLRTGKCVATLTGHTGEVN---SVAFSPDGEKLLSsssdgtIK------LWDLSTGKCLGTLRgHENG 221

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 277 FNsctmkSCCFAGDRDqkpeaegkviffrlclatkpifmdlrrprtktssqhYILSGSDDFNLYMWRIpadpeagGIGRV 356
Cdd:cd00200  222 VN-----SVAFSPDGY------------------------------------LLASGSEDGTIRVWDL-------RTGEC 253

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|
gi 578826238 357 VngafMVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:cd00200  254 V----QTLSGHTNSVTSLAWSPDGKRLASGSADGTIRIWD 289
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 140-171 2.76e-05

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 41.91  E-value: 2.76e-05
                           10        20        30
                   ....*....|....*....|....*....|..
gi 578826238   140 AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWD 171
Cdd:smart00320  10 GHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
49-81 1.40e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 40.02  E-value: 1.40e-04
                          10        20        30
                  ....*....|....*....|....*....|...
gi 578826238   49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLW 81
Cdd:pfam00400   7 LEGHTGSVTSLAFSPDG-KLLASGSDDGTVKVW 38
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 362-396 1.42e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 39.99  E-value: 1.42e-04
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 578826238   362 MVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:smart00320   6 KTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
PLN00181 PLN00181
protein SPA1-RELATED; Provisional
 19-224 1.42e-04

protein SPA1-RELATED; Provisional


Pssm-ID: 177776 [Multi-domain]  Cd Length: 793  Bit Score: 45.85  E-value: 1.42e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  19 SQRGLHGDPLLTQ-----DFQRRRLRGcrNLYKKDLLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHME------QAI 87
Cdd:PLN00181 446 SRQGGWIDPFLEGlckylSFSKLRVKA--DLKQGDLLNSSNLVCAIGFDRDG-EFFATAGVNKKIKIFECEsiikdgRDI 522

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  88 HSRVkpIQLKGEhhSNIFCLAFNSG-NTKVFSGGNDEQVILHDVESSETL-DVFAHEDAVYGLSVSPVNDNIFASSSDDG 165
Cdd:PLN00181 523 HYPV--VELASR--SKLSGICWNSYiKSQVASSNFEGVVQVWDVARSQLVtEMKEHEKRVWSIDYSSADPTLLASGSDDG 598

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 578826238 166 RVLIWDIREsphGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIRKPQSSL 224
Cdd:PLN00181 599 SVKLWSINQ---GVSIGTIKTKANICCVQFPSESGRSLAFGSADHKVYYYDLRNPKLPL 654
PTZ00420 PTZ00420
coronin; Provisional
 332-395 1.60e-04

coronin; Provisional


Pssm-ID: 240412 [Multi-domain]  Cd Length: 568  Bit Score: 45.33  E-value: 1.60e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 578826238 332 SGSDDFNLYMWRIPADPEAGgigRVVNGAFMVLKGHRSIVNQVRFNPHTYMI-CSSGVEKIIKIW 395
Cdd:PTZ00420  92 SGSEDLTIRVWEIPHNDESV---KEIKDPQCILKGHKKKISIIDWNPMNYYImCSSGFDSFVNIW 153
WD40 pfam00400
WD domain, G-beta repeat;
362-396 1.28e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 37.32  E-value: 1.28e-03
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 578826238  362 MVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:pfam00400   5 KTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 COG2319
WD40 repeat [General function prediction only];
49-250 8.84e-27

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 113.85  E-value: 8.84e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAihsrvKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:COG2319  158 LTGHSGAVTSVAFSPDG-KLLASGSDDGTVRLWDLATG-----KLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLW 231

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 129 DVESSETLDVFA-HEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIRESPHGEPfcLANYPSAFHSVMFNPvEPRLLATAN 207
Cdd:COG2319  232 DLATGKLLRTLTgHSGSVRSVAFSP-DGRLLASGSADGTVRLWDLATGELLRT--LTGHSGGVNSVAFSP-DGKLLASGS 307

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 578826238 208 SKEGVGLWDIRKPQsSLLRYGGNLSlqSAMSVRFNSNGTQLLA 250
Cdd:COG2319  308 DDGTVRLWDLATGK-LLRTLTGHTG--AVRSVAFSPDGKTLAS 347
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 49-396 1.30e-26

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 110.89  E-value: 1.30e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAIHSRvkpiQLKGeHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:cd00200    5 LKGHTGGVTCVAFSPDG-KLLATGSGDGTIKVWDLETGELLR----TLKG-HTGPVRDVAASADGTYLASGSSDKTIRLW 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 129 DVESSETLDVFA-HEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIRESPhgepfCLANYPSAFHSVM---FNPvEPRLLA 204
Cdd:cd00200   79 DLETGECVRTLTgHTSYVSSVAFSP-DGRILSSSSRDKTIKVWDVETGK-----CLTTLRGHTDWVNsvaFSP-DGTFVA 151

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 205 TAnSKEG-VGLWDIRKPQSSLLRYGGNLSLQsamSVRFNSNGTQLLA------LRrrlppvLYDIHSRLPVFQFD-NQGY 276
Cdd:cd00200  152 SS-SQDGtIKLWDLRTGKCVATLTGHTGEVN---SVAFSPDGEKLLSsssdgtIK------LWDLSTGKCLGTLRgHENG 221

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 277 FNsctmkSCCFAGDRDqkpeaegkviffrlclatkpifmdlrrprtktssqhYILSGSDDFNLYMWRIpadpeagGIGRV 356
Cdd:cd00200  222 VN-----SVAFSPDGY------------------------------------LLASGSEDGTIRVWDL-------RTGEC 253

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|
gi 578826238 357 VngafMVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:cd00200  254 V----QTLSGHTNSVTSLAWSPDGKRLASGSADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
3-397 1.44e-26

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 113.47  E-value: 1.44e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238   3 RRAGLGGSMRSVVGFLSQRGLHGDPLLTQDFQRRRLRGCRNLYKKDLLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWH 82
Cdd:COG2319   28 LLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDG-RLLASASADGTVRLWD 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  83 MEQAihsrvKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILHDVESSETLDVF-AHEDAVYGLSVSPvNDNIFASS 161
Cdd:COG2319  107 LATG-----LLLRTLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLtGHSGAVTSVAFSP-DGKLLASG 180

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 162 SDDGRVLIWDIREsphGEPFC-LANYPSAFHSVMFNPvEPRLLATANSKEGVGLWDIRKPQsSLLRYGGNLSlqSAMSVR 240
Cdd:COG2319  181 SDDGTVRLWDLAT---GKLLRtLTGHTGAVRSVAFSP-DGKLLASGSADGTVRLWDLATGK-LLRTLTGHSG--SVRSVA 253

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 241 FNSNGTQLLA------LRrrlppvLYDIHSRLPVFQFDNQgyfnSCTMKSCCF--------AGDRDqkpeaeGKVIFFRl 306
Cdd:COG2319  254 FSPDGRLLASgsadgtVR------LWDLATGELLRTLTGH----SGGVNSVAFspdgkllaSGSDD------GTVRLWD- 316

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 307 cLATKPIFMDLRRPRTKTSS------QHYILSGSDDFNLYMWripaDPEAGGIGRvvngafmVLKGHRSIVNQVRFNPHT 380
Cdd:COG2319  317 -LATGKLLRTLTGHTGAVRSvafspdGKTLASGSDDGTVRLW----DLATGELLR-------TLTGHTGAVTSVAFSPDG 384

                        410
                 ....*....|....*..
gi 578826238 381 YMICSSGVEKIIKIWSP 397
Cdd:COG2319  385 RTLASGSADGTVRLWDL 401
WD40 COG2319
WD40 repeat [General function prediction only];
49-173 7.42e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 93.05  E-value: 7.42e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHMEQAihsrvKPIQLKGEHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:COG2319  284 LTGHSGGVNSVAFSPDG-KLLASGSDDGTVRLWDLATG-----KLLRTLTGHTGAVRSVAFSPDGKTLASGSDDGTVRLW 357

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 578826238 129 DVESSETLDVF-AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIR 173
Cdd:COG2319  358 DLATGELLRTLtGHTGAVTSVAFSP-DGRTLASGSADGTVRLWDLA 402
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 95-434 1.67e-18

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 87.01  E-value: 1.67e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  95 QLKGeHHSNIFCLAFNSGNTKVFSGGNDEQVILHDVESSETLDVF-AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWDIR 173
Cdd:cd00200    4 TLKG-HTGGVTCVAFSPDGKLLATGSGDGTIKVWDLETGELLRTLkGHTGPVRDVAASA-DGTYLASGSSDKTIRLWDLE 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 174 ESPhgEPFCLANYPSAFHSVMFNPvEPRLLATANSKEGVGLWDIRKPQsSLLRYGGNLSlqSAMSVRFNSNGTQLLA--- 250
Cdd:cd00200   82 TGE--CVRTLTGHTSYVSSVAFSP-DGRILSSSSRDKTIKVWDVETGK-CLTTLRGHTD--WVNSVAFSPDGTFVASssq 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 251 ---LRrrlppvLYDIHSRLPVFQFdnqgYFNSCTMKSCCFAGDRDQkpeaegkviffrlclatkpifmdlrrprtktssq 327
Cdd:cd00200  156 dgtIK------LWDLRTGKCVATL----TGHTGEVNSVAFSPDGEK---------------------------------- 191

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238 328 hyILSGSDDFNLYMWRIPAdpeaggiGRVVngafMVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWspykqpgctgdl 407
Cdd:cd00200  192 --LLSSSSDGTIKLWDLST-------GKCL----GTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVW------------ 246

                        330       340
                 ....*....|....*....|....*..
gi 578826238 408 DGRIEDDSRCLYTHEEYISLVLNSGSG 434
Cdd:cd00200  247 DLRTGECVQTLSGHTNSVTSLAWSPDG 273
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 46-171 2.70e-14

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 74.68  E-value: 2.70e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  46 KKDLLGHFGCVNAIEFSNNGGQwLVSGGDDRRVLLW--HMEQAIHsrvkpiQLKGeHHSNIFCLAFNSGNTKVFSGGNDE 123
Cdd:cd00200  170 VATLTGHTGEVNSVAFSPDGEK-LLSSSSDGTIKLWdlSTGKCLG------TLRG-HENGVNSVAFSPDGYLLASGSEDG 241

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 578826238 124 QVILHDVESSETLDVF-AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWD 171
Cdd:cd00200  242 TIRVWDLRTGECVQTLsGHTNSVTSLAWSP-DGKRLASGSADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
49-132 2.80e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 2.80e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHmeqaIHSRVKPIQLKGeHHSNIFCLAFNSGNTKVFSGGNDEQVILH 128
Cdd:COG2319  326 LTGHTGAVRSVAFSPDG-KTLASGSDDGTVRLWD----LATGELLRTLTG-HTGAVTSVAFSPDGRTLASGSADGTVRLW 399


                 ....
gi 578826238 129 DVES 132
Cdd:COG2319  400 DLAT 403
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 140-171 2.76e-05

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 41.91  E-value: 2.76e-05
                           10        20        30
                   ....*....|....*....|....*....|..
gi 578826238   140 AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWD 171
Cdd:smart00320  10 GHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
49-81 1.40e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 40.02  E-value: 1.40e-04
                          10        20        30
                  ....*....|....*....|....*....|...
gi 578826238   49 LLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLW 81
Cdd:pfam00400   7 LEGHTGSVTSLAFSPDG-KLLASGSDDGTVKVW 38
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 362-396 1.42e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 39.99  E-value: 1.42e-04
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 578826238   362 MVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:smart00320   6 KTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
PLN00181 PLN00181
protein SPA1-RELATED; Provisional
 19-224 1.42e-04

protein SPA1-RELATED; Provisional


Pssm-ID: 177776 [Multi-domain]  Cd Length: 793  Bit Score: 45.85  E-value: 1.42e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  19 SQRGLHGDPLLTQ-----DFQRRRLRGcrNLYKKDLLGHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWHME------QAI 87
Cdd:PLN00181 446 SRQGGWIDPFLEGlckylSFSKLRVKA--DLKQGDLLNSSNLVCAIGFDRDG-EFFATAGVNKKIKIFECEsiikdgRDI 522

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  88 HSRVkpIQLKGEhhSNIFCLAFNSG-NTKVFSGGNDEQVILHDVESSETL-DVFAHEDAVYGLSVSPVNDNIFASSSDDG 165
Cdd:PLN00181 523 HYPV--VELASR--SKLSGICWNSYiKSQVASSNFEGVVQVWDVARSQLVtEMKEHEKRVWSIDYSSADPTLLASGSDDG 598

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 578826238 166 RVLIWDIREsphGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIRKPQSSL 224
Cdd:PLN00181 599 SVKLWSINQ---GVSIGTIKTKANICCVQFPSESGRSLAFGSADHKVYYYDLRNPKLPL 654
PTZ00420 PTZ00420
coronin; Provisional
 332-395 1.60e-04

coronin; Provisional


Pssm-ID: 240412 [Multi-domain]  Cd Length: 568  Bit Score: 45.33  E-value: 1.60e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 578826238 332 SGSDDFNLYMWRIPADPEAGgigRVVNGAFMVLKGHRSIVNQVRFNPHTYMI-CSSGVEKIIKIW 395
Cdd:PTZ00420  92 SGSEDLTIRVWEIPHNDESV---KEIKDPQCILKGHKKKISIIDWNPMNYYImCSSGFDSFVNIW 153
WD40 pfam00400
WD domain, G-beta repeat;
140-171 2.00e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 39.64  E-value: 2.00e-04
                          10        20        30
                  ....*....|....*....|....*....|..
gi 578826238  140 AHEDAVYGLSVSPvNDNIFASSSDDGRVLIWD 171
Cdd:pfam00400   9 GHTGSVTSLAFSP-DGKLLASGSDDGTVKVWD 39
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 51-82 8.28e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 37.68  E-value: 8.28e-04
                           10        20        30
                   ....*....|....*....|....*....|..
gi 578826238    51 GHFGCVNAIEFSNNGgQWLVSGGDDRRVLLWH 82
Cdd:smart00320  10 GHTGPVTSVAFSPDG-KYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
362-396 1.28e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 37.32  E-value: 1.28e-03
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 578826238  362 MVLKGHRSIVNQVRFNPHTYMICSSGVEKIIKIWS 396
Cdd:pfam00400   5 KTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
YncE COG3391
DNA-binding beta-propeller fold protein YncE [General function prediction only];
59-172 2.00e-03

DNA-binding beta-propeller fold protein YncE [General function prediction only];


Pssm-ID: 442618 [Multi-domain]  Cd Length: 237  Bit Score: 40.83  E-value: 2.00e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 578826238  59 IEFSNNGGQWLVSGGDDRRVLLwhMEQAIHSRVKPIQLKGEHHSnifcLAFNSGNTKVF---SGGNDEQVILH--DVESS 133
Cdd:COG3391  115 LAVDPDGGRLYVADSGNGRVSV--IDTATGKVVATIPVGAGPHG----IAVDPDGKRLYvanSGSNTVSVIVSviDTATG 188

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 578826238 134 ETLDVFAHEDAVYGLSVSPVNDNIF-------ASSSDDGRVLIWDI 172
Cdd:COG3391  189 KVVATIPVGGGPVGVAVSPDGRRLYvanrgsnTSNGGSNTVSVIDL 234
8prop_hemeD1_NirF cd20778
eight-bladed heme d1-binding beta-propeller domain in cytochrome cd1 nitrate reductase NirF; ...
 117-175 9.49e-03

eight-bladed heme d1-binding beta-propeller domain in cytochrome cd1 nitrate reductase NirF; Denitrification is a process that enables biofilm formation of the opportunistic human pathogen Pseudomonas aeruginosa, making it more resilient to antibiotics and highly adaptable to different habitats. During denitrification, nitrate (Nar), nitrite (Nir), nitric oxide (Nor), and nitrous oxide (Nos) reductases catalyze the reaction cascade of NO3- -> NO2- -> NO -> N2O -> N2. The integral membrane proteins NorC, NorB, and NosR form the core assembly platform that binds the nitrate reductase NarGHI and the periplasmic cytochrome cd1 (nitrite reductase) NirS via its maturation factor NirF. The nirFDLGHJE genes encode proteins required for heme d1 biosynthesis. NirS, NirF, and NirN, the monomeric dihydro-heme d1 dehydrogenase form a stable complex during nitrite reductase maturation. The nitrite reductase NirS is bound to the denitrification supercomplex via NorB, while the electron donor system NirM and the enzyme maturation machinery NirN-NirF-NirQ, interacting with NirS, are bound via NorC.


Pssm-ID: 467722 [Multi-domain]  Cd Length: 381  Bit Score: 39.57  E-value: 9.49e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 578826238 117 FSGGNDE--QVIlhDVESSETLDVFAHEDAVYGLSVSPVNDNIFASSSDDGRVLIWDIRES 175
Cdd:cd20778  299 FSGPDNDtvQVI--DTKTLKVVKTLEPGKRVLHMEFTPRGEAVYISVNDDNKVVVYDTRTF 357
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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