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Conserved domains on  [gi|1381410763|ref|XP_012775608|]
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chromatin assembly factor 1 subunit A isoform X3 [Maylandia zebra]

Protein Classification

CAF-1_p150 and CAF1A domain-containing protein( domain architecture ID 11701140)

protein containing domains CAF1-p150_N, CAF-1_p150, CAF1A, and CAF1-p150_C2

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CAF1-p150_C2 super family cl21343
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 597-876 4.92e-48

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


The actual alignment was detected with superfamily member pfam15539:

Pssm-ID: 434777  Cd Length: 288  Bit Score: 172.48  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 597 AREWDELMSSKKKMKVLEPMVRGCIW--EGEAPGFEL--LQSYVVCLVEPLPkADNSPSPEEmSLKSQREEKLLGKLLPL 672
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWaaDRDCAGADLkvLQQFTACLLETVP-SEEEQTPKA-SKREKRDQQILAQLLPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 673 LHGNLNSSKVIINEFREFCRQEI-----TSSSPPVLSSPQNPAEN--IPSRMQLRRLIKNNAIYEKRSAHRRCcWYVHQE 745
Cdd:pfam15539  79 LHGNVNGSKVIIREFQEHCRRGLlskgtGSPPSPSTSRPHTPTEDaaIPSKARLKRIISENSVYEKRPDFRMC-WYVHPQ 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 746 VLSRFGQEALPVPCQWTYLTTgareepreEPQAATGSQGNSPTTPQScSTTPSSSnKRKSTGSMSITKFMKRCNDPEQTE 825
Cdd:pfam15539 158 VLQSFDQEHLPVPCQWSYVTM--------VPSAPREDSGSVPATGPS-QGTPISL-KRKSAGSMCITQFMKKRRHDGQVG 227

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1381410763 826 AMEADGFQADTEDDDEDD--CVI-----ISTQSGPTKQTSSEGDGT-METTPSDTAALP 876
Cdd:pfam15539 228 AEDMDGFQADTEEEEEEDgdCMImdipdAGEVQAPCGTTSGAGGAVgMDTSKSPLPASP 286
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 487-530 3.35e-20

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


:

Pssm-ID: 463507  Cd Length: 75  Bit Score: 85.24  E-value: 3.35e-20
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1381410763 487 KLLQFRENYRPAYWGTWSKNSSHISPRRPFRQDKGLLDYEVDSD 530
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 255-411 1.58e-15

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


:

Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 75.11  E-value: 1.58e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 255 RRSLKSLQEQDERLRLRQEKERQKEEAKAAKEKKKEEARKLKEEREREKREKKEKDEREKREKKEKEERE---------- 324
Cdd:pfam11600   1 RRSQKSVQSQEEKEKQRLEKDKERLRRQLKLEAEKEEKERLKEEAKAEKERAKEEARRKKEEEKELKEKErrekkekdek 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 325 -KAERLKAKEELRKSKMEAKLEEKRKKEEEKRMKEEEKRLkeekDRLKAEKAEITRFLQKSKIQQAPKTLAAACGKFAPF 403
Cdd:pfam11600  81 eKAEKLRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEE----KRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPF 156


                  ....*...
gi 1381410763 404 EIKENMAL 411
Cdd:pfam11600 157 EIKEHMVL 164
CAF1-p150_N super family cl21359
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-113 7.29e-14

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


The actual alignment was detected with superfamily member pfam15557:

Pssm-ID: 464767  Cd Length: 230  Bit Score: 71.84  E-value: 7.29e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763   1 MDCKSSNNAS-KKLVQARLPFKRLNPEPKEN---------QPPKRPCAHSCPEPEVSDRENEN---ASSPLSVRsgPPLV 67
Cdd:pfam15557  19 MDCKDRPAFPvKKLIQARLPFKRLNLVPKEKaedgsddtrSSQSAPVQSQVPDLETSLDTLENnchMGSDIDFR--PKLV 96

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1381410763  68 NGRGPLDGFISRRRATTSDENM-IIDLTEDNTSSPLKPLASS---VSASP 113
Cdd:pfam15557  97 NGKGPLDNFLRSRVKTSIGQSMvIIDLTEDSSEQPDSTVAHSklnSAASP 146
 
Name Accession Description Interval E-value
CAF1-p150_C2 pfam15539
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 597-876 4.92e-48

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 434777  Cd Length: 288  Bit Score: 172.48  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 597 AREWDELMSSKKKMKVLEPMVRGCIW--EGEAPGFEL--LQSYVVCLVEPLPkADNSPSPEEmSLKSQREEKLLGKLLPL 672
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWaaDRDCAGADLkvLQQFTACLLETVP-SEEEQTPKA-SKREKRDQQILAQLLPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 673 LHGNLNSSKVIINEFREFCRQEI-----TSSSPPVLSSPQNPAEN--IPSRMQLRRLIKNNAIYEKRSAHRRCcWYVHQE 745
Cdd:pfam15539  79 LHGNVNGSKVIIREFQEHCRRGLlskgtGSPPSPSTSRPHTPTEDaaIPSKARLKRIISENSVYEKRPDFRMC-WYVHPQ 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 746 VLSRFGQEALPVPCQWTYLTTgareepreEPQAATGSQGNSPTTPQScSTTPSSSnKRKSTGSMSITKFMKRCNDPEQTE 825
Cdd:pfam15539 158 VLQSFDQEHLPVPCQWSYVTM--------VPSAPREDSGSVPATGPS-QGTPISL-KRKSAGSMCITQFMKKRRHDGQVG 227

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1381410763 826 AMEADGFQADTEDDDEDD--CVI-----ISTQSGPTKQTSSEGDGT-METTPSDTAALP 876
Cdd:pfam15539 228 AEDMDGFQADTEEEEEEDgdCMImdipdAGEVQAPCGTTSGAGGAVgMDTSKSPLPASP 286
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 487-530 3.35e-20

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


Pssm-ID: 463507  Cd Length: 75  Bit Score: 85.24  E-value: 3.35e-20
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1381410763 487 KLLQFRENYRPAYWGTWSKNSSHISPRRPFRQDKGLLDYEVDSD 530
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 255-411 1.58e-15

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 75.11  E-value: 1.58e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 255 RRSLKSLQEQDERLRLRQEKERQKEEAKAAKEKKKEEARKLKEEREREKREKKEKDEREKREKKEKEERE---------- 324
Cdd:pfam11600   1 RRSQKSVQSQEEKEKQRLEKDKERLRRQLKLEAEKEEKERLKEEAKAEKERAKEEARRKKEEEKELKEKErrekkekdek 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 325 -KAERLKAKEELRKSKMEAKLEEKRKKEEEKRMKEEEKRLkeekDRLKAEKAEITRFLQKSKIQQAPKTLAAACGKFAPF 403
Cdd:pfam11600  81 eKAEKLRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEE----KRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPF 156


                  ....*...
gi 1381410763 404 EIKENMAL 411
Cdd:pfam11600 157 EIKEHMVL 164
CAF1-p150_N pfam15557
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-113 7.29e-14

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 464767  Cd Length: 230  Bit Score: 71.84  E-value: 7.29e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763   1 MDCKSSNNAS-KKLVQARLPFKRLNPEPKEN---------QPPKRPCAHSCPEPEVSDRENEN---ASSPLSVRsgPPLV 67
Cdd:pfam15557  19 MDCKDRPAFPvKKLIQARLPFKRLNLVPKEKaedgsddtrSSQSAPVQSQVPDLETSLDTLENnchMGSDIDFR--PKLV 96

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1381410763  68 NGRGPLDGFISRRRATTSDENM-IIDLTEDNTSSPLKPLASS---VSASP 113
Cdd:pfam15557  97 NGKGPLDNFLRSRVKTSIGQSMvIIDLTEDSSEQPDSTVAHSklnSAASP 146
 
Name Accession Description Interval E-value
CAF1-p150_C2 pfam15539
CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the ...
 597-876 4.92e-48

CAF1 complex subunit p150, region binding to CAF1-p60 at C-term; CAF1-p150_C2 is part of the binding region of the CAF1 complex p150 subunit to the p60 subunit. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 434777  Cd Length: 288  Bit Score: 172.48  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 597 AREWDELMSSKKKMKVLEPMVRGCIW--EGEAPGFEL--LQSYVVCLVEPLPkADNSPSPEEmSLKSQREEKLLGKLLPL 672
Cdd:pfam15539   1 AKEWDEFLAKGKRFRVLQPVKIGCVWaaDRDCAGADLkvLQQFTACLLETVP-SEEEQTPKA-SKREKRDQQILAQLLPL 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 673 LHGNLNSSKVIINEFREFCRQEI-----TSSSPPVLSSPQNPAEN--IPSRMQLRRLIKNNAIYEKRSAHRRCcWYVHQE 745
Cdd:pfam15539  79 LHGNVNGSKVIIREFQEHCRRGLlskgtGSPPSPSTSRPHTPTEDaaIPSKARLKRIISENSVYEKRPDFRMC-WYVHPQ 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 746 VLSRFGQEALPVPCQWTYLTTgareepreEPQAATGSQGNSPTTPQScSTTPSSSnKRKSTGSMSITKFMKRCNDPEQTE 825
Cdd:pfam15539 158 VLQSFDQEHLPVPCQWSYVTM--------VPSAPREDSGSVPATGPS-QGTPISL-KRKSAGSMCITQFMKKRRHDGQVG 227

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1381410763 826 AMEADGFQADTEDDDEDD--CVI-----ISTQSGPTKQTSSEGDGT-METTPSDTAALP 876
Cdd:pfam15539 228 AEDMDGFQADTEEEEEEDgdCMImdipdAGEVQAPCGTTSGAGGAVgMDTSKSPLPASP 286
CAF1A pfam12253
Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of ...
 487-530 3.35e-20

Chromatin assembly factor 1 subunit A; The CAF-1 or chromatin assembly factor-1 consists of three subunits, and this is the first, or A. The A domain is uniquely required for the progression of S phase in mouse cells, independent of its ability to promote histone deposition but dependent on its ability to interact with HP1 - heterochromatin protein 1-rich heterochromatin domains next to centromeres that are crucial for chromosome segregation during mitosis. This HP1-CAF-1 interaction module functions as a built-in replication control for heterochromatin, which, like a control barrier, has an impact on S-phase progression in addition to DNA-based checkpoints.


Pssm-ID: 463507  Cd Length: 75  Bit Score: 85.24  E-value: 3.35e-20
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1381410763 487 KLLQFRENYRPAYWGTWSKNSSHISPRRPFRQDKGLLDYEVDSD 530
Cdd:pfam12253   1 KLLQFYEDVRPPYYGTWTKKSSVVGPRNPFAKDLLGLDYDYDSD 44
CAF-1_p150 pfam11600
Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide ...
 255-411 1.58e-15

Chromatin assembly factor 1 complex p150 subunit, N-terminal; CAF-1_p150 is a polypeptide subunit of CAF-1, which functions in depositing newly synthesized and acetylated histones H3/H4 into chromatin during DNA replication and repair. CAF-1_p150 includes the HP1 interaction site, the PEST, KER and ED interacting sites. CAF-1_p150 interacts directly with newly synthesized and acetylated histones through the acidic KER and ED domains. The PEST domain is associated with proteins that undergo rapid proteolysis.


Pssm-ID: 402959 [Multi-domain]  Cd Length: 164  Bit Score: 75.11  E-value: 1.58e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 255 RRSLKSLQEQDERLRLRQEKERQKEEAKAAKEKKKEEARKLKEEREREKREKKEKDEREKREKKEKEERE---------- 324
Cdd:pfam11600   1 RRSQKSVQSQEEKEKQRLEKDKERLRRQLKLEAEKEEKERLKEEAKAEKERAKEEARRKKEEEKELKEKErrekkekdek 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763 325 -KAERLKAKEELRKSKMEAKLEEKRKKEEEKRMKEEEKRLkeekDRLKAEKAEITRFLQKSKIQQAPKTLAAACGKFAPF 403
Cdd:pfam11600  81 eKAEKLRLKEEKRKEKQEALEAKLEEKRKKEEEKRLKEEE----KRIKAEKAEITRFLQKPKTQQAPKTLAGSCGKFAPF 156


                  ....*...
gi 1381410763 404 EIKENMAL 411
Cdd:pfam11600 157 EIKEHMVL 164
CAF1-p150_N pfam15557
CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of ...
 1-113 7.29e-14

CAF1 complex subunit p150, region binding to PCNA; CAF1-p150_N is part of the N-terminus of the CAF1 complex p150 subunit that binds to PCNA - proliferating cell nuclear antigen. The PCNA mediates the connection between CAF-1 and the DNA replication fork. The CAF1 complex is essential in human cells for the de novo deposition of histones H3 and H4 at the DNA replication fork.


Pssm-ID: 464767  Cd Length: 230  Bit Score: 71.84  E-value: 7.29e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1381410763   1 MDCKSSNNAS-KKLVQARLPFKRLNPEPKEN---------QPPKRPCAHSCPEPEVSDRENEN---ASSPLSVRsgPPLV 67
Cdd:pfam15557  19 MDCKDRPAFPvKKLIQARLPFKRLNLVPKEKaedgsddtrSSQSAPVQSQVPDLETSLDTLENnchMGSDIDFR--PKLV 96

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1381410763  68 NGRGPLDGFISRRRATTSDENM-IIDLTEDNTSSPLKPLASS---VSASP 113
Cdd:pfam15557  97 NGKGPLDNFLRSRVKTSIGQSMvIIDLTEDSSEQPDSTVAHSklnSAASP 146
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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