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Conserved domains on  [gi|2462562948|ref|XP_054175797|]
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F-box only protein 27 isoform X3 [Homo sapiens]

Protein Classification

F-box protein( domain architecture ID 18135419)

F-box protein functions as the substrate-recognition component of an SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligase that is involved in ubiquitin-dependent degradation

CATH:  1.20.1280.50
Gene Ontology:  GO:0006511|GO:0019005|GO:0005515
PubMed:  31898225|11178263
SCOP:  4001927|4000817

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FBA pfam04300
F-box associated region; Members of this family are associated with F-box domains, hence the ...
 105-236 3.00e-79

F-box associated region; Members of this family are associated with F-box domains, hence the name FBA. This domain is probably involved in binding other proteins that will be targeted for ubiquitination. Swiss:Q9UK22 is involved in binding to N-glycosylated proteins.


:

Pssm-ID: 461255  Cd Length: 175  Bit Score: 235.93  E-value: 3.00e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562948 105 CARRPIGRNLIRNPCGQEGLRKW-MVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGR 183
Cdd:pfam04300   1 YFLRPLGRNLIKNPCGEEGLKHWeIVENGGDGWKVEENGEPLPDASVQKCFVTSYRWCLKSQLIDLKAEGLWPELLDSFQ 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2462562948 184 IEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLH 236
Cdd:pfam04300  81 PEIKVSDWYAARQDCGCIYELHVQLLDANKTVLAEFSPEPVPIPQWNDNQWFQ 133
F-box_SF super family cl45894
F-box domain superfamily; This short domain is commonly found at the N-terminus of various ...
 39-73 1.10e-11

F-box domain superfamily; This short domain is commonly found at the N-terminus of various proteins, and typically co-occurs with one or more other conserved domains or motifs, such as leucine rich repeats, WD40 repeats, kelch, tub, spry, and others. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression. One of the best researched roles of F-box proteins is their participation in SCF (Skp1-Cul1-F-box protein), a multi-protein complex that functions as a ubiquitin E3 ligase, where the role of the F-box protein is to recruit target substrates. Gene families containing the F-box are found greatly expanded in narrow taxonomic lineages, such as flowering plants and nematodes. In this hierarchical classification, many of the subfamilies are named according to their domain architectures.


The actual alignment was detected with superfamily member cd22169:

Pssm-ID: 459239  Cd Length: 48  Bit Score: 58.20  E-value: 1.10e-11
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVDGQALWLLILAR 73
Cdd:cd22169    14 SHVPARTLVTRCRLVCRDWRDLVDGPTLWKLKCER 48
 
Name Accession Description Interval E-value
FBA pfam04300
F-box associated region; Members of this family are associated with F-box domains, hence the ...
 105-236 3.00e-79

F-box associated region; Members of this family are associated with F-box domains, hence the name FBA. This domain is probably involved in binding other proteins that will be targeted for ubiquitination. Swiss:Q9UK22 is involved in binding to N-glycosylated proteins.


Pssm-ID: 461255  Cd Length: 175  Bit Score: 235.93  E-value: 3.00e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562948 105 CARRPIGRNLIRNPCGQEGLRKW-MVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGR 183
Cdd:pfam04300   1 YFLRPLGRNLIKNPCGEEGLKHWeIVENGGDGWKVEENGEPLPDASVQKCFVTSYRWCLKSQLIDLKAEGLWPELLDSFQ 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2462562948 184 IEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLH 236
Cdd:pfam04300  81 PEIKVSDWYAARQDCGCIYELHVQLLDANKTVLAEFSPEPVPIPQWNDNQWFQ 133
F-box_FBXO17-like cd22169
F-box domain found in F-box only protein 17 (FBXO17), F-box only protein 27 (FBXO27) and ...
 39-73 1.10e-11

F-box domain found in F-box only protein 17 (FBXO17), F-box only protein 27 (FBXO27) and similar proteins; This subfamily includes FBXO17 and FBXO27. FBXO17 is also called FBX17, F-box only protein 26 (FBX26/FBXO26), or FBG4, while FBXO27 is also called FBX27, or F-box/G-domain protein 5. FBXO17 and FBXO27 are the substrate-recognition components of SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. They can recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. FBXO17 also recognizes sulfated glycans but does not bind high-mannose glycoproteins. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438940  Cd Length: 48  Bit Score: 58.20  E-value: 1.10e-11
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVDGQALWLLILAR 73
Cdd:cd22169    14 SHVPARTLVTRCRLVCRDWRDLVDGPTLWKLKCER 48
FBOX smart00256
A Receptor for Ubiquitination Targets;
39-67 8.12e-04

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 36.26  E-value: 8.12e-04
                           10        20
                   ....*....|....*....|....*....
gi 2462562948   39 SHVPPRTLLgRCRQVCRGWRALVDGQALW 67
Cdd:smart00256  11 SKLDPKDLL-RLRKVSRKWRSLIDSHDFW 38
F-box-like pfam12937
F-box-like; This is an F-box-like family.
 39-67 1.10e-03

F-box-like; This is an F-box-like family.


Pssm-ID: 463757 [Multi-domain]  Cd Length: 45  Bit Score: 35.92  E-value: 1.10e-03
                          10        20
                  ....*....|....*....|....*....
gi 2462562948  39 SHVPPRTLLgRCRQVCRGWRALVDGQALW 67
Cdd:pfam12937  14 SYLDPKDLL-RLALVCRRWRELASDDSLW 41
 
Name Accession Description Interval E-value
FBA pfam04300
F-box associated region; Members of this family are associated with F-box domains, hence the ...
 105-236 3.00e-79

F-box associated region; Members of this family are associated with F-box domains, hence the name FBA. This domain is probably involved in binding other proteins that will be targeted for ubiquitination. Swiss:Q9UK22 is involved in binding to N-glycosylated proteins.


Pssm-ID: 461255  Cd Length: 175  Bit Score: 235.93  E-value: 3.00e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562948 105 CARRPIGRNLIRNPCGQEGLRKW-MVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGR 183
Cdd:pfam04300   1 YFLRPLGRNLIKNPCGEEGLKHWeIVENGGDGWKVEENGEPLPDASVQKCFVTSYRWCLKSQLIDLKAEGLWPELLDSFQ 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2462562948 184 IEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLH 236
Cdd:pfam04300  81 PEIKVSDWYAARQDCGCIYELHVQLLDANKTVLAEFSPEPVPIPQWNDNQWFQ 133
F-box_FBXO17-like cd22169
F-box domain found in F-box only protein 17 (FBXO17), F-box only protein 27 (FBXO27) and ...
 39-73 1.10e-11

F-box domain found in F-box only protein 17 (FBXO17), F-box only protein 27 (FBXO27) and similar proteins; This subfamily includes FBXO17 and FBXO27. FBXO17 is also called FBX17, F-box only protein 26 (FBX26/FBXO26), or FBG4, while FBXO27 is also called FBX27, or F-box/G-domain protein 5. FBXO17 and FBXO27 are the substrate-recognition components of SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. They can recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides. FBXO17 also recognizes sulfated glycans but does not bind high-mannose glycoproteins. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438940  Cd Length: 48  Bit Score: 58.20  E-value: 1.10e-11
                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVDGQALWLLILAR 73
Cdd:cd22169    14 SHVPARTLVTRCRLVCRDWRDLVDGPTLWKLKCER 48
F-box_FBXO6-like cd22168
F-box domain found in F-box only proteins FBXO6, FBXO44 and similar proteins; This subfamily ...
 39-118 9.12e-06

F-box domain found in F-box only proteins FBXO6, FBXO44 and similar proteins; This subfamily includes FBXO6 and FBXO44. FBXO6, also called FBX6, F-box protein that recognizes sugar chains 2 (FBS2), or F-box/G-domain protein 2 (FBG2), is a substrate-recognition component of SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. It is involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins, by recognizing and binding sugar chains on unfolded glycoproteins that are retro-translocated into the cytosol and promoting their ubiquitination and subsequent degradation. FBXO6 can recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. It also recognizes sulfated glycans. FBXO6 is involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. FBXO44, also called FBXO6A, FBX44, or F-box/G-domain protein 3 (FBG3), is a substrate-recognition component of an SCF-type E3 ubiquitin ligase complex. It interacts with SKP1 and CUL1. FBXO44 mediates BRCA1 ubiquitination and degradation. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438939 [Multi-domain]  Cd Length: 82  Bit Score: 42.66  E-value: 9.12e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVDGQALWllilardhgatGRALLHLARSCQSPARNarPCPLGRFCARRpIGRNLIRNP 118
Cdd:cd22168    17 SLVPARDLILSCRLVCSRWRDLVDLPTLW-----------KRKCQREGFILKDWDGP--PKDWKIFYFLR-LERNLLKNP 82
F-box_FBA cd22083
F-box domain found in the F-box associated (FBA) family of F-box proteins; The F-box ...
 39-62 1.78e-04

F-box domain found in the F-box associated (FBA) family of F-box proteins; The F-box associated (FBA) family is composed of FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44, which contain a conserved G domain that mediates substrate binding. Members of this family play diverse roles in glycoprotein quality control. They bind high mannose and sulfated glycoproteins, with one FBA protein, FBXO44, failing to bind any glycans on the tested arrays. FBA proteins are components of canonical SCF (Skp1, Cullin1, and Rbx1) complexes, yet FBXO2 bound very little Cullin1, suggesting that FBXO2 may exist primarily as a heterodimer with Skp1. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438855  Cd Length: 37  Bit Score: 38.14  E-value: 1.78e-04
                          10        20
                  ....*....|....*....|....
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVD 62
Cdd:cd22083    14 THVPATSLITNCRLVCTLWRDIID 37
F-box_FBXO2 cd22167
F-box domain found in F-box only protein 2 (FBXO2) and similar proteins; FBXO2, also called ...
 39-69 2.13e-04

F-box domain found in F-box only protein 2 (FBXO2) and similar proteins; FBXO2, also called FBX2, is a neuron-specific substrate-recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It binds specifically to proteins attached with N-linked high-mannose type oligosaccharides, and subsequently contributes to ubiquitination of glycoproteins. FBXO2 regulates amyloid precursor protein (APP) levels and processing in the brain, and may play a role in modulating Alzheimer disease (AD) pathogenesis. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438938 [Multi-domain]  Cd Length: 72  Bit Score: 38.57  E-value: 2.13e-04
                          10        20        30
                  ....*....|....*....|....*....|.
gi 2462562948  39 SHVPPRTLLGRCRQVCRGWRALVDGQALWLL 69
Cdd:cd22167    14 AYLPAEELVLVCRLVCSQWKELVDGAALWIL 44
FBOX smart00256
A Receptor for Ubiquitination Targets;
39-67 8.12e-04

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 36.26  E-value: 8.12e-04
                           10        20
                   ....*....|....*....|....*....
gi 2462562948   39 SHVPPRTLLgRCRQVCRGWRALVDGQALW 67
Cdd:smart00256  11 SKLDPKDLL-RLRKVSRKWRSLIDSHDFW 38
F-box-like pfam12937
F-box-like; This is an F-box-like family.
 39-67 1.10e-03

F-box-like; This is an F-box-like family.


Pssm-ID: 463757 [Multi-domain]  Cd Length: 45  Bit Score: 35.92  E-value: 1.10e-03
                          10        20
                  ....*....|....*....|....*....
gi 2462562948  39 SHVPPRTLLgRCRQVCRGWRALVDGQALW 67
Cdd:pfam12937  14 SYLDPKDLL-RLALVCRRWRELASDDSLW 41
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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