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Conserved domains on  [gi|2462548477|ref|XP_054236042|]
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trinucleotide repeat-containing gene 6A protein isoform X30 [Homo sapiens]

Protein Classification

TNRC6-PABC_bdg and RRM_TNRC6A domain-containing protein( domain architecture ID 11244329)

TNRC6-PABC_bdg and RRM_TNRC6A domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TNRC6-PABC_bdg pfam16608
TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher ...
 1364-1625 9.87e-103

TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher eukaryote TNRC6 subset of GW182 proteins that carries the binding motif for the interaction with Polyadenylate-binding protein 1, PABC. TNRC6 are trinucleotide repeat-containing gene 6 proteins required for miRNA-mediated gene silencing that are localized to the P bodies (processing bodies). P bodies are cytoplasmic mRNP aggregates that are involved in general mRNA translation repression and decay, including nonsense-mediated decay. Thus GW182 proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells being a major component of miRISCs. The interaction motif that binds to PABC is ShNWPPEFHPGVPWKGLQ. This region lies between a Q-rich region and the RRM, or RNA-recognition motif, pfam13893.


:

Pssm-ID: 465195  Cd Length: 290  Bit Score: 331.18  E-value: 9.87e-103
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1364 NAFSNFPI-GLNSNLNV-NMDMN---SIKEP--QSRLRKWT-TVDSISVNTS-LDQNSSKHGAISSGFRLEESPFVPYDF 1434
Cdd:pfam16608    3 NTFSPYPLaGLNPNMNVsNMDITgglGGKEPqsQSRLKQWTnSMDNLSSAASpLDQNSSKHGAISAGLRLEDSSFGPYDL 82

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1435 MNSSTSPASPPGSIGDGWPRAKSPN----GSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDH- 1509
Cdd:pfam16608   83 IPGSESPASPPGPVGDSWPRAKSPPdkisNSSNVNWPPEFRPGVPWKGLQNIDPETDPYVTPGSVINGLSINTIRDTDHq 162

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1510 -LRDRNSGSSSSLNTTLPSTSAWsSIRASNYNVPLSSTAQSTSARNSDSKLTWSPG--SVTNTSLAHELWKVPLPPKNIT 1586
Cdd:pfam16608  163 lLRDRNNGPSSSLNTTLPSNSAW-PISASNHSSSLSSTASSTSAKLSDSKSTWSPGpiSHTQASLSHELWKVPLPPRNTT 241

                          250       260       270
                   ....*....|....*....|....*....|....*....
gi 2462548477 1587 APSRPPPGLTGQKPPlSTWDNSPLRIgGGWGNSDARYTP 1625
Cdd:pfam16608  242 APTRPPPGLTNQKPS-STWGASALRL-GGWGSSESRYSS 278
RRM_TNRC6A cd12711
RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds ...
 1633-1724 6.80e-55

RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds to the RRM of the GW182 autoantigen, also termed trinucleotide repeat-containing gene 6A protein (TNRC6A), or CAG repeat protein 26, or EMSY interactor protein, or protein GW1, or glycine-tryptophan protein of 182 kDa, a phosphorylated cytoplasmic autoantigen involved in stabilizing and/or regulating translation and/or storing several different mRNAs. GW182 is characterized by multiple glycine/tryptophan (G/W) repeats and is a critical component of GW bodies (GWBs, also called mammalian processing bodies, or P bodies). The mRNAs associated with GW182 are presumed to reside within GWBs. GW182 has been shown to bind multiple Ago-miRNA complexes, and thus plays a key role in miRNA-mediated translational repression and mRNA degradation. In the absence of Ago2, GW182 may induce translational silencing effect. GW182 is composed of an N-terminal G/W-rich region containing an Ago hook responsible for Ago protein-binding; a ubiquitin-associated (UBA) domain and a glutamine (Q)-rich region in the middle region; a middle G/W-rich region, a RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain), and a C-terminal G/W-rich region, at the C-terminus. A bipartite C-terminal region including the middle and C-terminal G/W-rich regions is referred to as silencing domain that triggers silencing of bound transcripts by inhibiting protein expression and promoting mRNA decay via deadenylation.


:

Pssm-ID: 410110  Cd Length: 92  Bit Score: 186.05  E-value: 6.80e-55
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1633 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEE 1712
Cdd:cd12711      1 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEAVKAQKSLHMCVLGNTTILAEFASEE 80

                           90
                   ....*....|..
gi 2462548477 1713 EISRFFAQSQSL 1724
Cdd:cd12711     81 EISRFFAQGQSL 92
ser_rich_anae_1 super family cl41472
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 405-693 1.22e-06

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


The actual alignment was detected with superfamily member NF033849:

Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 53.86  E-value: 1.22e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  405 QSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSlpnSGSVQNNELPSSNTGAWR 484
Cdd:NF033849   245 ESVGHSTSQGQSHSVGTSESHSVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSV---GTSESQSHGTTEGTSTTD 321

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  485 VSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATlmqPGVNGPMGtnfq 564
Cdd:NF033849   322 SSSHSQSSSYNVSSGTGVSSSHSDGTSQSTSISHSESSSESTGTSVGHSTSSSVSSSESSSRSSS---SGVSGGFS---- 394

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  565 vNTNKGGGVWESGAANSQSTSWgsgngansggsrrGWGTpaqNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKST 644
Cdd:NF033849   395 -GGIAGGGVTSEGLGASQGGSE-------------GWGS---GDSVQSVSQSYGSSSSTGTSSGHSDSSSHSTSSGQADS 457

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 2462548477  645 E------EEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSR 693
Cdd:NF033849   458 VsqgtswSEGTGTSQGQSVGTSESWSTSQSETDSVGDSTGTSESVSQGDGRSTGR 512
ser_rich_anae_1 super family cl41472
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 622-860 1.22e-04

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


The actual alignment was detected with superfamily member NF033849:

Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 47.31  E-value: 1.22e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  622 SNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSV----WAKTGGTVESDGS--TESTGRLEEKGTGESQSrdr 695
Cdd:NF033849   266 SVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSVGTSesqsHGTTEGTSTTDSSshSQSSSYNVSSGTGVSSS--- 342

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  696 rkidqhtllqsivnrtdldprvLSNSGWGQTPIKQNTAWdTETSPRGERKTDNGTEAWGSSATQTFNSGacidktspngn 775
Cdd:NF033849   343 ----------------------HSDGTSQSTSISHSESS-SESTGTSVGHSTSSSVSSSESSSRSSSSG----------- 388

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  776 dtSSVSGWGDPKPALRWGDSKGSNcQGGWEDDSAATGMVKSNQ-WGNCKEEKAAW--------------NDSQKNKQGWG 840
Cdd:NF033849   389 --VSGGFSGGIAGGGVTSEGLGAS-QGGSEGWGSGDSVQSVSQsYGSSSSTGTSSghsdssshstssgqADSVSQGTSWS 465

                          250       260
                   ....*....|....*....|
gi 2462548477  841 DGQKSSQGWSVSASDNWGET 860
Cdd:NF033849   466 EGTGTSQGQSVGTSESWSTS 485
 
Name Accession Description Interval E-value
TNRC6-PABC_bdg pfam16608
TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher ...
 1364-1625 9.87e-103

TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher eukaryote TNRC6 subset of GW182 proteins that carries the binding motif for the interaction with Polyadenylate-binding protein 1, PABC. TNRC6 are trinucleotide repeat-containing gene 6 proteins required for miRNA-mediated gene silencing that are localized to the P bodies (processing bodies). P bodies are cytoplasmic mRNP aggregates that are involved in general mRNA translation repression and decay, including nonsense-mediated decay. Thus GW182 proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells being a major component of miRISCs. The interaction motif that binds to PABC is ShNWPPEFHPGVPWKGLQ. This region lies between a Q-rich region and the RRM, or RNA-recognition motif, pfam13893.


Pssm-ID: 465195  Cd Length: 290  Bit Score: 331.18  E-value: 9.87e-103
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1364 NAFSNFPI-GLNSNLNV-NMDMN---SIKEP--QSRLRKWT-TVDSISVNTS-LDQNSSKHGAISSGFRLEESPFVPYDF 1434
Cdd:pfam16608    3 NTFSPYPLaGLNPNMNVsNMDITgglGGKEPqsQSRLKQWTnSMDNLSSAASpLDQNSSKHGAISAGLRLEDSSFGPYDL 82

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1435 MNSSTSPASPPGSIGDGWPRAKSPN----GSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDH- 1509
Cdd:pfam16608   83 IPGSESPASPPGPVGDSWPRAKSPPdkisNSSNVNWPPEFRPGVPWKGLQNIDPETDPYVTPGSVINGLSINTIRDTDHq 162

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1510 -LRDRNSGSSSSLNTTLPSTSAWsSIRASNYNVPLSSTAQSTSARNSDSKLTWSPG--SVTNTSLAHELWKVPLPPKNIT 1586
Cdd:pfam16608  163 lLRDRNNGPSSSLNTTLPSNSAW-PISASNHSSSLSSTASSTSAKLSDSKSTWSPGpiSHTQASLSHELWKVPLPPRNTT 241

                          250       260       270
                   ....*....|....*....|....*....|....*....
gi 2462548477 1587 APSRPPPGLTGQKPPlSTWDNSPLRIgGGWGNSDARYTP 1625
Cdd:pfam16608  242 APTRPPPGLTNQKPS-STWGASALRL-GGWGSSESRYSS 278
RRM_TNRC6A cd12711
RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds ...
 1633-1724 6.80e-55

RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds to the RRM of the GW182 autoantigen, also termed trinucleotide repeat-containing gene 6A protein (TNRC6A), or CAG repeat protein 26, or EMSY interactor protein, or protein GW1, or glycine-tryptophan protein of 182 kDa, a phosphorylated cytoplasmic autoantigen involved in stabilizing and/or regulating translation and/or storing several different mRNAs. GW182 is characterized by multiple glycine/tryptophan (G/W) repeats and is a critical component of GW bodies (GWBs, also called mammalian processing bodies, or P bodies). The mRNAs associated with GW182 are presumed to reside within GWBs. GW182 has been shown to bind multiple Ago-miRNA complexes, and thus plays a key role in miRNA-mediated translational repression and mRNA degradation. In the absence of Ago2, GW182 may induce translational silencing effect. GW182 is composed of an N-terminal G/W-rich region containing an Ago hook responsible for Ago protein-binding; a ubiquitin-associated (UBA) domain and a glutamine (Q)-rich region in the middle region; a middle G/W-rich region, a RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain), and a C-terminal G/W-rich region, at the C-terminus. A bipartite C-terminal region including the middle and C-terminal G/W-rich regions is referred to as silencing domain that triggers silencing of bound transcripts by inhibiting protein expression and promoting mRNA decay via deadenylation.


Pssm-ID: 410110  Cd Length: 92  Bit Score: 186.05  E-value: 6.80e-55
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1633 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEE 1712
Cdd:cd12711      1 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEAVKAQKSLHMCVLGNTTILAEFASEE 80

                           90
                   ....*....|..
gi 2462548477 1713 EISRFFAQSQSL 1724
Cdd:cd12711     81 EISRFFAQGQSL 92
ser_rich_anae_1 NF033849
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 405-693 1.22e-06

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 53.86  E-value: 1.22e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  405 QSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSlpnSGSVQNNELPSSNTGAWR 484
Cdd:NF033849   245 ESVGHSTSQGQSHSVGTSESHSVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSV---GTSESQSHGTTEGTSTTD 321

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  485 VSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATlmqPGVNGPMGtnfq 564
Cdd:NF033849   322 SSSHSQSSSYNVSSGTGVSSSHSDGTSQSTSISHSESSSESTGTSVGHSTSSSVSSSESSSRSSS---SGVSGGFS---- 394

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  565 vNTNKGGGVWESGAANSQSTSWgsgngansggsrrGWGTpaqNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKST 644
Cdd:NF033849   395 -GGIAGGGVTSEGLGASQGGSE-------------GWGS---GDSVQSVSQSYGSSSSTGTSSGHSDSSSHSTSSGQADS 457

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 2462548477  645 E------EEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSR 693
Cdd:NF033849   458 VsqgtswSEGTGTSQGQSVGTSESWSTSQSETDSVGDSTGTSESVSQGDGRSTGR 512
ser_rich_anae_1 NF033849
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 622-860 1.22e-04

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 47.31  E-value: 1.22e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  622 SNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSV----WAKTGGTVESDGS--TESTGRLEEKGTGESQSrdr 695
Cdd:NF033849   266 SVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSVGTSesqsHGTTEGTSTTDSSshSQSSSYNVSSGTGVSSS--- 342

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  696 rkidqhtllqsivnrtdldprvLSNSGWGQTPIKQNTAWdTETSPRGERKTDNGTEAWGSSATQTFNSGacidktspngn 775
Cdd:NF033849   343 ----------------------HSDGTSQSTSISHSESS-SESTGTSVGHSTSSSVSSSESSSRSSSSG----------- 388

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  776 dtSSVSGWGDPKPALRWGDSKGSNcQGGWEDDSAATGMVKSNQ-WGNCKEEKAAW--------------NDSQKNKQGWG 840
Cdd:NF033849   389 --VSGGFSGGIAGGGVTSEGLGAS-QGGSEGWGSGDSVQSVSQsYGSSSSTGTSSghsdssshstssgqADSVSQGTSWS 465

                          250       260
                   ....*....|....*....|
gi 2462548477  841 DGQKSSQGWSVSASDNWGET 860
Cdd:NF033849   466 EGTGTSQGQSVGTSESWSTS 485
COG4625 COG4625
Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain [Function ...
 267-687 6.01e-03

Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain [Function unknown];


Pssm-ID: 443664 [Multi-domain]  Cd Length: 900  Bit Score: 41.69  E-value: 6.01e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  267 NITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWG 346
Cdd:COG4625     91 GGVGGGGGGGGGGGGGGGGGGGGGGGGSAGGGGGGAGGAGGGGGGGAGGGGGGGGGGGAGGGGGGGAGGAGGGGGGGGGG 170

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  347 TVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQET 426
Cdd:COG4625    171 GGGGGGGGGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGG 250

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  427 CESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSH 506
Cdd:COG4625    251 GGGGGGGNGGGGGAGGGGGGGGGGSGGGGGGGGGGGSGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 330

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  507 LSNGESKS-------GGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAA 579
Cdd:COG4625    331 GGGAGGGGgsggagaGGGGAGGGGAGGGGGGGTGGGGGGGGGGGGGSGGGGAGGGGGSGGGGGGGAGGGGGGGGAGGTGG 410

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  580 NSQS-TSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNE 658
Cdd:COG4625    411 GGAGgGGGAAGGGGGGTGAGGGGGGGGTGAGGGGATGGGGGGGGGAGGSGGGAGAGGGSGSGAGTLTLTGNNTYTGTTTV 490

                          410       420       430
                   ....*....|....*....|....*....|.
gi 2462548477  659 QS--SVWAKTGGTVESDGSTESTGRLEEKGT 687
Cdd:COG4625    491 NGggNYTQSAGSTLAVEVDAANSDRLVVTGT 521
 
Name Accession Description Interval E-value
TNRC6-PABC_bdg pfam16608
TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher ...
 1364-1625 9.87e-103

TNRC6-PABC binding domain; TNRC6-PABC_bdg is a natively unstructured region on the higher eukaryote TNRC6 subset of GW182 proteins that carries the binding motif for the interaction with Polyadenylate-binding protein 1, PABC. TNRC6 are trinucleotide repeat-containing gene 6 proteins required for miRNA-mediated gene silencing that are localized to the P bodies (processing bodies). P bodies are cytoplasmic mRNP aggregates that are involved in general mRNA translation repression and decay, including nonsense-mediated decay. Thus GW182 proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells being a major component of miRISCs. The interaction motif that binds to PABC is ShNWPPEFHPGVPWKGLQ. This region lies between a Q-rich region and the RRM, or RNA-recognition motif, pfam13893.


Pssm-ID: 465195  Cd Length: 290  Bit Score: 331.18  E-value: 9.87e-103
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1364 NAFSNFPI-GLNSNLNV-NMDMN---SIKEP--QSRLRKWT-TVDSISVNTS-LDQNSSKHGAISSGFRLEESPFVPYDF 1434
Cdd:pfam16608    3 NTFSPYPLaGLNPNMNVsNMDITgglGGKEPqsQSRLKQWTnSMDNLSSAASpLDQNSSKHGAISAGLRLEDSSFGPYDL 82

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1435 MNSSTSPASPPGSIGDGWPRAKSPN----GSSSVNWPPEFRPGEPWKGYPNIDPETDPYVTPGSVINNLSINTVREVDH- 1509
Cdd:pfam16608   83 IPGSESPASPPGPVGDSWPRAKSPPdkisNSSNVNWPPEFRPGVPWKGLQNIDPETDPYVTPGSVINGLSINTIRDTDHq 162

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1510 -LRDRNSGSSSSLNTTLPSTSAWsSIRASNYNVPLSSTAQSTSARNSDSKLTWSPG--SVTNTSLAHELWKVPLPPKNIT 1586
Cdd:pfam16608  163 lLRDRNNGPSSSLNTTLPSNSAW-PISASNHSSSLSSTASSTSAKLSDSKSTWSPGpiSHTQASLSHELWKVPLPPRNTT 241

                          250       260       270
                   ....*....|....*....|....*....|....*....
gi 2462548477 1587 APSRPPPGLTGQKPPlSTWDNSPLRIgGGWGNSDARYTP 1625
Cdd:pfam16608  242 APTRPPPGLTNQKPS-STWGASALRL-GGWGSSESRYSS 278
RRM_TNRC6A cd12711
RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds ...
 1633-1724 6.80e-55

RNA recognition motif (RRM) found in vertebrate GW182 autoantigen; This subgroup corresponds to the RRM of the GW182 autoantigen, also termed trinucleotide repeat-containing gene 6A protein (TNRC6A), or CAG repeat protein 26, or EMSY interactor protein, or protein GW1, or glycine-tryptophan protein of 182 kDa, a phosphorylated cytoplasmic autoantigen involved in stabilizing and/or regulating translation and/or storing several different mRNAs. GW182 is characterized by multiple glycine/tryptophan (G/W) repeats and is a critical component of GW bodies (GWBs, also called mammalian processing bodies, or P bodies). The mRNAs associated with GW182 are presumed to reside within GWBs. GW182 has been shown to bind multiple Ago-miRNA complexes, and thus plays a key role in miRNA-mediated translational repression and mRNA degradation. In the absence of Ago2, GW182 may induce translational silencing effect. GW182 is composed of an N-terminal G/W-rich region containing an Ago hook responsible for Ago protein-binding; a ubiquitin-associated (UBA) domain and a glutamine (Q)-rich region in the middle region; a middle G/W-rich region, a RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain), and a C-terminal G/W-rich region, at the C-terminus. A bipartite C-terminal region including the middle and C-terminal G/W-rich regions is referred to as silencing domain that triggers silencing of bound transcripts by inhibiting protein expression and promoting mRNA decay via deadenylation.


Pssm-ID: 410110  Cd Length: 92  Bit Score: 186.05  E-value: 6.80e-55
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1633 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEE 1712
Cdd:cd12711      1 SSGRITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEAVKAQKSLHMCVLGNTTILAEFASEE 80

                           90
                   ....*....|..
gi 2462548477 1713 EISRFFAQSQSL 1724
Cdd:cd12711     81 EISRFFAQGQSL 92
RRM_TNRC6C cd12713
RNA recognition motif (RRM) found in vertebrate trinucleotide repeat-containing gene 6C ...
 1636-1720 1.94e-48

RNA recognition motif (RRM) found in vertebrate trinucleotide repeat-containing gene 6C protein (TNRC6C); This subgroup corresponds to the RRM of TNRC6C, one of three GW182 paralogs in mammalian genomes. It is enriched in P-bodies and important for efficient miRNA-mediated repression. TNRC6C is composed of an N-terminal glycine/tryptophan (G/W)-rich region containing an Ago hook responsible for Ago protein-binding; a ubiquitin-associated (UBA) domain and a glutamine (Q)-rich region in the middle region; a middle G/W-rich region, a RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain), and a C-terminal G/W-rich region, at the C-terminus. A bipartite C-terminal region including the middle and C-terminal G/W-rich regions is referred as silencing domain that triggers silencing of bound transcripts by inhibiting protein expression and promoting mRNA decay via deadenylation. The C-terminal half containing the RRM domain functions as a key effector domain mediating protein synthesis repression by TNRC6C.


Pssm-ID: 410112 [Multi-domain]  Cd Length: 88  Bit Score: 167.19  E-value: 1.94e-48
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1636 RITNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEIS 1715
Cdd:cd12713      4 RTSSWLVLRNLTPQIDGSTLRTLCLQHGPLITFHLNLTQGNAVVRYSSKEEAAKAQKSLHMCVLGNTTILAEFASEEEVN 83


                   ....*
gi 2462548477 1716 RFFAQ 1720
Cdd:cd12713     84 RFLAQ 88
RRM_TNRC6B cd12712
RNA recognition motif (RRM) found in vertebrate trinucleotide repeat-containing gene 6B ...
 1640-1720 2.20e-47

RNA recognition motif (RRM) found in vertebrate trinucleotide repeat-containing gene 6B protein (TNRC6B); This subgroup corresponds to the RRM of TNRC6B, one of three GW182 paralogs in mammalian genomes. It is involved in miRNA-mediated mRNA degradation. TNRC6B is composed of an N-terminal glycine/tryptophan (G/W)-rich region; a ubiquitin-associated (UBA) domain and a glutamine (Q)-rich region in the middle region; a middle G/W-rich region, a RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain), and a C-terminal G/W-rich region, at the C-terminus. TNRC6B directly interacts with Argonaute (Ago) proteins through its N-terminal glycine/tryptophan (G/W)-rich region that is called Ago protein-binding domain. TNRC6B is enriched in P-bodies and its Q-rich domain is responsible for P-body localization. A bipartite C-terminal region including the middle and C-terminal G/W-rich regions is referred as silencing domain that triggers silencing of bound transcripts by inhibiting protein expression and promoting mRNA decay via deadenylation. The C-terminal half of TNRC6B comprising an RRM domain exerts a strong translation inhibition potential, which does not require either association with Agos or localization to P-bodies.


Pssm-ID: 410111  Cd Length: 83  Bit Score: 164.08  E-value: 2.20e-47
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477 1640 WLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEFASEEEISRFFA 1719
Cdd:cd12712      3 WLVLHNLTPQIDGSTLRTICMQHGPLLTFHLNLTQGTALIRYSTKQEAAKAQTALHMCVLGNTTILAEFATEEEVSRYFA 82


                   .
gi 2462548477 1720 Q 1720
Cdd:cd12712     83 Q 83
RRM_GW182_like cd12435
RNA recognition motif (RRM) found in the GW182 family proteins; This subfamily corresponds to ...
 1638-1708 4.11e-47

RNA recognition motif (RRM) found in the GW182 family proteins; This subfamily corresponds to the RRM of the GW182 family which includes three paralogs of TNRC6 (GW182-related) proteins comprising GW182/TNGW1, TNRC6B (containing three isoforms) and TNRC6C in mammal, a single Drosophila ortholog (dGW182, also called Gawky) and two Caenorhabditis elegans orthologs AIN-1 and AIN-2, which contain multiple miRNA-binding sites and have important functions in miRNA-mediated translational repression, as well as mRNA degradation in Metazoa. The GW182 family proteins directly interact with Argonaute (Ago) proteins, and thus function as downstream effectors in the miRNA pathway, responsible for inhibition of translation and acceleration of mRNA decay. Members in this family are characterized by an abnormally high content of glycine/tryptophan (G/W) repeats, one or more glutamine (Q)-rich motifs, and a C-terminal RNA recognition motif (RRM), also termed RBD (RNA binding domain) or RNP (ribonucleoprotein domain). The only exception is the worm protein that does not contain a recognizable RRM domain. The GW182 family proteins are recruited to miRNA targets through an interaction between their N-terminal domain and an Argonaute protein. Then they promote translational repression and/or degradation of miRNA targets through their C-terminal silencing domain.


Pssm-ID: 409869 [Multi-domain]  Cd Length: 71  Bit Score: 162.99  E-value: 4.11e-47
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2462548477 1638 TNWLVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPHGNALVRYSSKEEVVKAQKSLHMCVLGNTTILAEF 1708
Cdd:cd12435      1 SNWLVLRNLTPQIDGSTLRTLCMQHGPLLTFHLNLNHGNALIRYSSREEAAKAQKALNMCVLGNTTILADF 71
ser_rich_anae_1 NF033849
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 405-693 1.22e-06

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 53.86  E-value: 1.22e-06
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  405 QSINSKVSGGSTHGTWGSLQETCESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSlpnSGSVQNNELPSSNTGAWR 484
Cdd:NF033849   245 ESVGHSTSQGQSHSVGTSESHSVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSV---GTSESQSHGTTEGTSTTD 321

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  485 VSTMNHPQMQAPSGMNGTSLSHLSNGESKSGGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATlmqPGVNGPMGtnfq 564
Cdd:NF033849   322 SSSHSQSSSYNVSSGTGVSSSHSDGTSQSTSISHSESSSESTGTSVGHSTSSSVSSSESSSRSSS---SGVSGGFS---- 394

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  565 vNTNKGGGVWESGAANSQSTSWgsgngansggsrrGWGTpaqNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKST 644
Cdd:NF033849   395 -GGIAGGGVTSEGLGASQGGSE-------------GWGS---GDSVQSVSQSYGSSSSTGTSSGHSDSSSHSTSSGQADS 457

                          250       260       270       280       290
                   ....*....|....*....|....*....|....*....|....*....|....*
gi 2462548477  645 E------EEDQGSATSQTNEQSSVWAKTGGTVESDGSTESTGRLEEKGTGESQSR 693
Cdd:NF033849   458 VsqgtswSEGTGTSQGQSVGTSESWSTSQSETDSVGDSTGTSESVSQGDGRSTGR 512
ser_rich_anae_1 NF033849
serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 ...
 622-860 1.22e-04

serine-rich protein; This serine-rich protein belongs to a family with large size (over 1000 amino acids), which a highly serine-rich central region that averages over 300 aa in length. Species encoding members of this family of proteins tend to be anaerobic bacteria, including Gram-positive bacteria of the human gut microbiome and Chloroflexi from marine sediments.


Pssm-ID: 468206 [Multi-domain]  Cd Length: 1122  Bit Score: 47.31  E-value: 1.22e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  622 SNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNEQSSV----WAKTGGTVESDGS--TESTGRLEEKGTGESQSrdr 695
Cdd:NF033849   266 SVGTSQSQSHTTGHGSTRGWSHTQSTSESESTGQSSSVGTSesqsHGTTEGTSTTDSSshSQSSSYNVSSGTGVSSS--- 342

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  696 rkidqhtllqsivnrtdldprvLSNSGWGQTPIKQNTAWdTETSPRGERKTDNGTEAWGSSATQTFNSGacidktspngn 775
Cdd:NF033849   343 ----------------------HSDGTSQSTSISHSESS-SESTGTSVGHSTSSSVSSSESSSRSSSSG----------- 388

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  776 dtSSVSGWGDPKPALRWGDSKGSNcQGGWEDDSAATGMVKSNQ-WGNCKEEKAAW--------------NDSQKNKQGWG 840
Cdd:NF033849   389 --VSGGFSGGIAGGGVTSEGLGAS-QGGSEGWGSGDSVQSVSQsYGSSSSTGTSSghsdssshstssgqADSVSQGTSWS 465

                          250       260
                   ....*....|....*....|
gi 2462548477  841 DGQKSSQGWSVSASDNWGET 860
Cdd:NF033849   466 EGTGTSQGQSVGTSESWSTS 485
RRM_SF cd00590
RNA recognition motif (RRM) superfamily; RRM, also known as RBD (RNA binding domain) or RNP ...
 1641-1704 2.61e-04

RNA recognition motif (RRM) superfamily; RRM, also known as RBD (RNA binding domain) or RNP (ribonucleoprotein domain), is a highly abundant domain in eukaryotes found in proteins involved in post-transcriptional gene expression processes including mRNA and rRNA processing, RNA export, and RNA stability. This domain is 90 amino acids in length and consists of a four-stranded beta-sheet packed against two alpha-helices. RRM usually interacts with ssRNA, but is also known to interact with ssDNA as well as proteins. RRM binds a variable number of nucleotides, ranging from two to eight. The active site includes three aromatic side-chains located within the conserved RNP1 and RNP2 motifs of the domain. The RRM domain is found in a variety heterogeneous nuclear ribonucleoproteins (hnRNPs), proteins implicated in regulation of alternative splicing, and protein components of small nuclear ribonucleoproteins (snRNPs).


Pssm-ID: 409669 [Multi-domain]  Cd Length: 72  Bit Score: 41.11  E-value: 2.61e-04
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2462548477 1641 LVLKNLTPQIDGSTLRTLCMQHGPLITFHLNLPH-----GNALVRYSSKEEVVKAQKSLHMCVLGNTTI 1704
Cdd:cd00590      1 LFVGNLPPDTTEEDLRELFSKFGEVVSVRIVRDRdgkskGFAFVEFESPEDAEKALEALNGTELGGRPL 69
COG4625 COG4625
Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain [Function ...
 267-687 6.01e-03

Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain [Function unknown];


Pssm-ID: 443664 [Multi-domain]  Cd Length: 900  Bit Score: 41.69  E-value: 6.01e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  267 NITIMASGNTGGEKDGLRNSTGLGSQNKFVVGSSSNNVGHGSSTGPWGFSHGAIISTCQVSVDAPESKSESSNNRMNAWG 346
Cdd:COG4625     91 GGVGGGGGGGGGGGGGGGGGGGGGGGGSAGGGGGGAGGAGGGGGGGAGGGGGGGGGGGAGGGGGGGAGGAGGGGGGGGGG 170

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  347 TVSSSSNGGLNPSTLNSASNHGAWPVLENNGLALKGPVGSGSSGINIQCSTIGQMPNNQSINSKVSGGSTHGTWGSLQET 426
Cdd:COG4625    171 GGGGGGGGGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGG 250

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  427 CESEVSGTQKVSFSGQPQNITTEMTGPNNTTNFMTSSLPNSGSVQNNELPSSNTGAWRVSTMNHPQMQAPSGMNGTSLSH 506
Cdd:COG4625    251 GGGGGGGNGGGGGAGGGGGGGGGGSGGGGGGGGGGGSGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 330

                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  507 LSNGESKS-------GGSYGTTWGAYGSNYSGDKCSGPNGQANGDTVNATLMQPGVNGPMGTNFQVNTNKGGGVWESGAA 579
Cdd:COG4625    331 GGGAGGGGgsggagaGGGGAGGGGAGGGGGGGTGGGGGGGGGGGGGSGGGGAGGGGGSGGGGGGGAGGGGGGGGAGGTGG 410

                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462548477  580 NSQS-TSWGSGNGANSGGSRRGWGTPAQNTGTNLPSVEWNKLPSNQHSNDSANGNGKTFTNGWKSTEEEDQGSATSQTNE 658
Cdd:COG4625    411 GGAGgGGGAAGGGGGGTGAGGGGGGGGTGAGGGGATGGGGGGGGGAGGSGGGAGAGGGSGSGAGTLTLTGNNTYTGTTTV 490

                          410       420       430
                   ....*....|....*....|....*....|.
gi 2462548477  659 QS--SVWAKTGGTVESDGSTESTGRLEEKGT 687
Cdd:COG4625    491 NGggNYTQSAGSTLAVEVDAANSDRLVVTGT 521
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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