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Conserved domains on  [gi|2047440836|ref|YP_010095669|]
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baseplate wedge subunit [Aeromonas phage 50AhydR13PP]

Protein Classification

25 family protein( domain architecture ID 10009692)

25 family protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
25 PHA00415
baseplate wedge subunit
 1-127 3.29e-65

baseplate wedge subunit


:

Pssm-ID: 222788  Cd Length: 131  Bit Score: 193.68  E-value: 3.29e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836   1 MNINLLYTDLDPDLNKS-WDNDASMTRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTA 79
Cdd:PHA00415    2 MNINQLYSDIDPNFKKSpWNKDVSAVKGARAIKNSLLGIVTTRKGERPFDPNFGCDISDQLFENMDPLTADTIERNIESA 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2047440836  80 IRNFEPRVYNLQVDSTGNYDNNELIVTIRFSIIDNPAVIDEIKMKLSN 127
Cdd:PHA00415   82 IRNYEPRVYNLNVEVIPVYDDNSIIVTIYFSIIDNPDDLEQIKFQLSR 129
 
Name Accession Description Interval E-value
25 PHA00415
baseplate wedge subunit
 1-127 3.29e-65

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 193.68  E-value: 3.29e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836   1 MNINLLYTDLDPDLNKS-WDNDASMTRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTA 79
Cdd:PHA00415    2 MNINQLYSDIDPNFKKSpWNKDVSAVKGARAIKNSLLGIVTTRKGERPFDPNFGCDISDQLFENMDPLTADTIERNIESA 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2047440836  80 IRNFEPRVYNLQVDSTGNYDNNELIVTIRFSIIDNPAVIDEIKMKLSN 127
Cdd:PHA00415   82 IRNYEPRVYNLNVEVIPVYDDNSIIVTIYFSIIDNPDDLEQIKFQLSR 129
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
24-114 8.36e-19

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 75.68  E-value: 8.36e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836  24 MTRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTAIRNFEPRVYNLQVD-STGNYDNNE 102
Cdd:COG3628    15 MVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEVESVDvTPDPEEDGK 94

                          90
                  ....*....|..
gi 2047440836 103 LIVTIRFSIIDN 114
Cdd:COG3628    95 LLITIDYTVRAT 106
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 25-116 1.94e-17

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 71.46  E-value: 1.94e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836  25 TRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTAIRNFEPRVYNLQVD-STGNYDNNEL 103
Cdd:pfam04965   1 SSLRESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEvDPDPDSPGTL 80

                          90
                  ....*....|...
gi 2047440836 104 IVTIRFSIIDNPA 116
Cdd:pfam04965  81 RFTIEYTLRATPA 93
 
Name Accession Description Interval E-value
25 PHA00415
baseplate wedge subunit
 1-127 3.29e-65

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 193.68  E-value: 3.29e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836   1 MNINLLYTDLDPDLNKS-WDNDASMTRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTA 79
Cdd:PHA00415    2 MNINQLYSDIDPNFKKSpWNKDVSAVKGARAIKNSLLGIVTTRKGERPFDPNFGCDISDQLFENMDPLTADTIERNIESA 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2047440836  80 IRNFEPRVYNLQVDSTGNYDNNELIVTIRFSIIDNPAVIDEIKMKLSN 127
Cdd:PHA00415   82 IRNYEPRVYNLNVEVIPVYDDNSIIVTIYFSIIDNPDDLEQIKFQLSR 129
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
24-114 8.36e-19

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 75.68  E-value: 8.36e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836  24 MTRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTAIRNFEPRVYNLQVD-STGNYDNNE 102
Cdd:COG3628    15 MVSGEEHIRQSIRIILTTPPGERVMRPDFGSGLHDLLFEPNNPATALLIRAAVAEALLRWEPRIEVESVDvTPDPEEDGK 94

                          90
                  ....*....|..
gi 2047440836 103 LIVTIRFSIIDN 114
Cdd:COG3628    95 LLITIDYTVRAT 106
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 25-116 1.94e-17

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 71.46  E-value: 1.94e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2047440836  25 TRGARAVKNSIIGIVSTPIGARPFNPYFGCDLPDQLFENITPLVIDTVRRNIQTAIRNFEPRVYNLQVD-STGNYDNNEL 103
Cdd:pfam04965   1 SSLRESVRRDLEILLNTRPGERVSRPNYGCPDLALLFAPNDDATRALIERAIREAILRFEPRIRVVSVEvDPDPDSPGTL 80

                          90
                  ....*....|...
gi 2047440836 104 IVTIRFSIIDNPA 116
Cdd:pfam04965  81 RFTIEYTLRATPA 93
COG3518 COG3518
Predicted component of the type VI protein secretion system [Intracellular trafficking, ...
 72-111 5.09e-04

Predicted component of the type VI protein secretion system [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442740  Cd Length: 162  Bit Score: 37.65  E-value: 5.09e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 2047440836  72 VRRNIQTAIRNFEPRVYNLQVDSTGNYDNNELivTIRFSI 111
Cdd:COG3518    94 IERAIRQAILRFEPRLRRVSVTVLPDDDDSPN--TLRFRI 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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