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Conserved domains on  [gi|4507909|ref|NP_000368|]
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actin nucleation-promoting factor WAS [Homo sapiens]

Protein Classification

Wiskott-Aldrich syndrome protein( domain architecture ID 11099496)

Wiskott-Aldrich syndrome protein (WASp) acts as an effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex

Gene Ontology:  GO:0003779|GO:0031267|GO:0030695|GO:0030048|GO:2000601
PubMed:  9883880

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
 36-145 1.66e-54

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


:

Pssm-ID: 395450  Cd Length: 111  Bit Score: 178.41  E-value: 1.66e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909     36 FEMLGRKCLTLATAVVQLYLALPPGAEHWTK-EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFF 114
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADPDNKRHWIKaKHSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQARPFF 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 4507909    115 HTFAGDDCQAGLNFADEDEAQAFRALVQEKI 145
Cdd:pfam00568  81 HTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
 237-294 4.77e-16

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


:

Pssm-ID: 395634  Cd Length: 59  Bit Score: 72.34  E-value: 4.77e-16
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 4507909    237 DIGAPSGFKHVSHVGWDPQNGFDVnNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFI 294
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFFT-GLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
 
Name Accession Description Interval E-value
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
 36-145 1.66e-54

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 178.41  E-value: 1.66e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909     36 FEMLGRKCLTLATAVVQLYLALPPGAEHWTK-EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFF 114
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADPDNKRHWIKaKHSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQARPFF 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 4507909    115 HTFAGDDCQAGLNFADEDEAQAFRALVQEKI 145
Cdd:pfam00568  81 HTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
 45-145 5.99e-52

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 171.17  E-value: 5.99e-52
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909   45 TLATAVVQLYLALPPgAEHWTK-EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQ 123
Cdd:cd01205   1 ILAAAVARLYYAEPD-PDSWSYtGLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|..
gi 4507909  124 AGLNFADEDEAQAFRALVQEKI 145
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEKL 101
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
 39-145 2.23e-38

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 135.56  E-value: 2.23e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909      39 LGRKCLTLATAVVQLYLALPPGaehWTKEHCG-AVCFVKDNPQKSYFIRLYGLQAG-RLLWEQELYSQLVYSTPTPFFHT 116
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK---WVPTGEGgAANLVIDKNQRSYFFRIVGIKGQdKVIWNQELYKNFKYNQATPTFHQ 77

                           90       100
                   ....*....|....*....|....*....
gi 4507909     117 FAGDDCQAGLNFADEDEAQAFRALVQEKI 145
Cdd:smart00461  78 WADDKCVYGLNFASEEEAKKFRKKVLKAL 106
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
 237-294 4.77e-16

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 395634  Cd Length: 59  Bit Score: 72.34  E-value: 4.77e-16
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 4507909    237 DIGAPSGFKHVSHVGWDPQNGFDVnNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFI 294
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFFT-GLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
CRIB cd00132
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
 236-278 7.50e-14

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.


Pssm-ID: 238077  Cd Length: 42  Bit Score: 65.54  E-value: 7.50e-14
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 4507909  236 ADIGAPSGFKHVSHVGWDPQnGFDVNNLDPDLRSLFSRAGISE 278
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGV-GFDGANLPPDLQSLFQTAGISA 42
PBD smart00285
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
 238-273 6.47e-08

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 197628  Cd Length: 36  Bit Score: 48.36  E-value: 6.47e-08
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 4507909     238 IGAPSGFKHVSHVGWDPQNGfDVNNLDPDLRSLFSR 273
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG-GFTGLPTEWKSLLKT 35
 
Name Accession Description Interval E-value
WH1 pfam00568
WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in ...
 36-145 1.66e-54

WH1 domain; WASp Homology domain 1 (WH1) domain. WASP is the protein that is defective in Wiskott-Aldrich syndrome (WAS). The majority of point mutations occur within the amino- terminal WH1 domain. The metabotropic glutamate receptors mGluR1alpha and mGluR5 bind a protein called homer, which is a WH1 domain homolog. A subset of WH1 domains has been termed a "EVH1" domain and appear to bind a polyproline motif.


Pssm-ID: 395450  Cd Length: 111  Bit Score: 178.41  E-value: 1.66e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909     36 FEMLGRKCLTLATAVVQLYLALPPGAEHWTK-EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFF 114
Cdd:pfam00568   1 FSALGKKCQTICTAVAQVYLADPDNKRHWIKaKHSGVVCFVKDSPQNSYFIRLVDIQDGKVIWNQEIYPNMEYNQARPFF 80

                          90       100       110
                  ....*....|....*....|....*....|.
gi 4507909    115 HTFAGDDCQAGLNFADEDEAQAFRALVQEKI 145
Cdd:pfam00568  81 HTFADSRCVYGLNFASEEEATKFAKAVQEAL 111
EVH1_WASP-like cd01205
WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called ...
 45-145 5.99e-52

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269916  Cd Length: 101  Bit Score: 171.17  E-value: 5.99e-52
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909   45 TLATAVVQLYLALPPgAEHWTK-EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQ 123
Cdd:cd01205   1 ILAAAVARLYYAEPD-PDSWSYtGLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|..
gi 4507909  124 AGLNFADEDEAQAFRALVQEKI 145
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEKL 101
WH1 smart00461
WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains ...
 39-145 2.23e-38

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.


Pssm-ID: 214674  Cd Length: 106  Bit Score: 135.56  E-value: 2.23e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909      39 LGRKCLTLATAVVQLYLALPPGaehWTKEHCG-AVCFVKDNPQKSYFIRLYGLQAG-RLLWEQELYSQLVYSTPTPFFHT 116
Cdd:smart00461   1 LGSQCIILARAVVQLYDADTKK---WVPTGEGgAANLVIDKNQRSYFFRIVGIKGQdKVIWNQELYKNFKYNQATPTFHQ 77

                           90       100
                   ....*....|....*....|....*....
gi 4507909     117 FAGDDCQAGLNFADEDEAQAFRALVQEKI 145
Cdd:smart00461  78 WADDKCVYGLNFASEEEAKKFRKKVLKAL 106
EVH1_family cd00837
EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; ...
 45-145 3.65e-22

EVH1 (Drosophila Enabled (Ena)/Vasodilator-stimulated phosphoprotein (VASP) homology 1) domain; The EVH1 domains are part of the PH domain superfamily. EVH1 subfamilies include Enables/VASP, Homer/Vesl, WASP, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.


Pssm-ID: 269909  Cd Length: 103  Bit Score: 90.98  E-value: 3.65e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4507909   45 TLATAVVQLYlALPPGAEHWTK---EHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDD 121
Cdd:cd00837   1 SIFSARAHVM-QIDDSNKNWVPaggKGASRVSYFKDTTRNSFRIIGVDIKDKKVVINCTITKNLVYNKATQTFHQWADDR 79

                        90       100
                ....*....|....*....|....
gi 4507909  122 CQAGLNFADEDEAQAFRALVQEKI 145
Cdd:cd00837  80 TVFGLNFASEEDATKFAEAVQEAL 103
PBD pfam00786
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
 237-294 4.77e-16

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 395634  Cd Length: 59  Bit Score: 72.34  E-value: 4.77e-16
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 4507909    237 DIGAPSGFKHVSHVGWDPQNGFDVnNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFI 294
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFFT-GLPPEWAKLLDSSGITEDEQKENPKAVLDVLKF 57
CRIB cd00132
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
 236-278 7.50e-14

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.


Pssm-ID: 238077  Cd Length: 42  Bit Score: 65.54  E-value: 7.50e-14
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 4507909  236 ADIGAPSGFKHVSHVGWDPQnGFDVNNLDPDLRSLFSRAGISE 278
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGV-GFDGANLPPDLQSLFQTAGISA 42
PBD smart00285
P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also ...
 238-273 6.47e-08

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).


Pssm-ID: 197628  Cd Length: 36  Bit Score: 48.36  E-value: 6.47e-08
                           10        20        30
                   ....*....|....*....|....*....|....*.
gi 4507909     238 IGAPSGFKHVSHVGWDPQNGfDVNNLDPDLRSLFSR 273
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG-GFTGLPTEWKSLLKT 35
CRIB_PAK_like cd01093
PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; ...
 238-257 9.43e-05

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. This subgroup of CRIB/PBD-domains is found N-terminal of Serine/Threonine kinase domains in PAK and PAK-like proteins.


Pssm-ID: 238526  Cd Length: 46  Bit Score: 39.95  E-value: 9.43e-05
                        10        20
                ....*....|....*....|
gi 4507909  238 IGAPSGFKHVSHVGWDPQNG 257
Cdd:cd01093   3 ISSPTNFKHRVHVGFDPQTG 22
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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