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Conserved domains on  [gi|55956773|ref|NP_001007235|]
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DNA excision repair protein ERCC-8 isoform 3 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 40-184 3.14e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 63.89  E-value: 3.14e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  40 RIHGGGINTLDIEPVeGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPHDTGMFtSSS 119
Cdd:cd00200   6 KGHTGGVTCVAFSPD-GKLLATGSGDGTIKVWDLETGELLRTLK-------------GHTGPVRDVAASADGTYLA-SGS 70

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 55956773 120 FDKTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200  71 SDKTIRLWDLETGECVRTLTgHTSYVSSVAFSPDGR---ILSSSSRDKTIKVWDVETGKCLTTLRG 133
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 40-184 3.14e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 63.89  E-value: 3.14e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  40 RIHGGGINTLDIEPVeGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPHDTGMFtSSS 119
Cdd:cd00200   6 KGHTGGVTCVAFSPD-GKLLATGSGDGTIKVWDLETGELLRTLK-------------GHTGPVRDVAASADGTYLA-SGS 70

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 55956773 120 FDKTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200  71 SDKTIRLWDLETGECVRTLTgHTSYVSSVAFSPDGR---ILSSSSRDKTIKVWDVETGKCLTTLRG 133
WD40 COG2319
WD40 repeat [General function prediction only];
42-184 1.03e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 59.92  E-value: 1.03e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPhDTGMFTSSSFD 121
Cdd:COG2319 161 HSGAVTSVAFSP-DGKLLASGSDDGTVRLWDLATGKLLRTLT-------------GHTGAVRSVAFSP-DGKLLASGSAD 225

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 55956773 122 KTLKVWDTNTLQTADVFNFEE-TVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:COG2319 226 GTVRLWDLATGKLLRTLTGHSgSVRSVAFSPDGR---LLASGSADGTVRLWDLATGELLRTLTG 286
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 40-184 3.14e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 63.89  E-value: 3.14e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  40 RIHGGGINTLDIEPVeGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPHDTGMFtSSS 119
Cdd:cd00200   6 KGHTGGVTCVAFSPD-GKLLATGSGDGTIKVWDLETGELLRTLK-------------GHTGPVRDVAASADGTYLA-SGS 70

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 55956773 120 FDKTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200  71 SDKTIRLWDLETGECVRTLTgHTSYVSSVAFSPDGR---ILSSSSRDKTIKVWDVETGKCLTTLRG 133
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 56-184 4.89e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 60.43  E-value: 4.89e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  56 GRYMLSGGSDGVIVLYDLENSSRQSYYTCkavcsigrdhpdvHRYSVETVQWYPHDTgMFTSSSFDKTLKVWDTNTLQTA 135
Cdd:cd00200  63 GTYLASGSSDKTIRLWDLETGECVRTLTG-------------HTSYVSSVAFSPDGR-ILSSSSRDKTIKVWDVETGKCL 128

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|
gi 55956773 136 DVFNF-EETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200 129 TTLRGhTDWVNSVAFSPDGT---FVASSSQDGTIKLWDLRTGKCVATLTG 175
WD40 COG2319
WD40 repeat [General function prediction only];
42-184 1.03e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 59.92  E-value: 1.03e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPhDTGMFTSSSFD 121
Cdd:COG2319 161 HSGAVTSVAFSP-DGKLLASGSDDGTVRLWDLATGKLLRTLT-------------GHTGAVRSVAFSP-DGKLLASGSAD 225

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 55956773 122 KTLKVWDTNTLQTADVFNFEE-TVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:COG2319 226 GTVRLWDLATGKLLRTLTGHSgSVRSVAFSPDGR---LLASGSADGTVRLWDLATGELLRTLTG 286
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 42-184 1.58e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 58.89  E-value: 1.58e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENssrqsyYTCKAVCsigRDHPDvhrySVETVQWyPHDTGMFTSSSFD 121
Cdd:cd00200  92 HTSYVSSVAFSP-DGRILSSSSRDKTIKVWDVET------GKCLTTL---RGHTD----WVNSVAF-SPDGTFVASSSQD 156

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 55956773 122 KTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTKhclVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200 157 GTIKLWDLRTGKCVATLTgHTGEVNSVAFSPDGEK---LLSSSSDGTIKLWDLSTGKCLGTLRG 217
WD40 COG2319
WD40 repeat [General function prediction only];
42-184 8.12e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 57.23  E-value: 8.12e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPhDTGMFTSSSFD 121
Cdd:COG2319 245 HSGSVRSVAFSP-DGRLLASGSADGTVRLWDLATGELLRTLT-------------GHSGGVNSVAFSP-DGKLLASGSDD 309

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 55956773 122 KTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:COG2319 310 GTVRLWDLATGKLLRTLTgHTGAVRSVAFSPDGK---TLASGSDDGTVRLWDLATGELLRTLTG 370
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 42-184 1.50e-09

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.19  E-value: 1.50e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSrqsyytCKAVCSIgrdhpdvHRYSVETVQWYPhDTGMFTSSSFD 121
Cdd:cd00200 134 HTDWVNSVAFSP-DGTFVASSSQDGTIKLWDLRTGK------CVATLTG-------HTGEVNSVAFSP-DGEKLLSSSSD 198

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 55956773 122 KTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLKSGSCSHILQG 184
Cdd:cd00200 199 GTIKLWDLSTGKCLGTLRgHENGVNSVAFSPDGY---LLASGSEDGTIRVWDLRTGECVQTLSG 259
WD40 COG2319
WD40 repeat [General function prediction only];
16-184 4.52e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 55.30  E-value: 4.52e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  16 LRLRRAESTRRVLGLELNKDRDVERIHGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSRQSyytckavcsigrdHP 95
Cdd:COG2319  51 LAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSP-DGRLLASASADGTVRLWDLATGLLLR-------------TL 116

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  96 DVHRYSVETVQWYPhDTGMFTSSSFDKTLKVWDTNTLQTADVFN-FEETVYSHHMSPVSTkhcLVAVGTRGPKVQLCDLK 174
Cdd:COG2319 117 TGHTGAVRSVAFSP-DGKTLASGSADGTVRLWDLATGKLLRTLTgHSGAVTSVAFSPDGK---LLASGSDDGTVRLWDLA 192

                       170
                ....*....|
gi 55956773 175 SGSCSHILQG 184
Cdd:COG2319 193 TGKLLRTLTG 202
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 42-151 3.83e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 51.95  E-value: 3.83e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLenSSRQSYYTCKAvcsigrdhpdvHRYSVETVQWYPHDtGMFTSSSFD 121
Cdd:cd00200 176 HTGEVNSVAFSP-DGEKLLSSSSDGTIKLWDL--STGKCLGTLRG-----------HENGVNSVAFSPDG-YLLASGSED 240

                        90       100       110
                ....*....|....*....|....*....|.
gi 55956773 122 KTLKVWDTNTLQTADVF-NFEETVYSHHMSP 151
Cdd:cd00200 241 GTIRVWDLRTGECVQTLsGHTNSVTSLAWSP 271
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 40-128 1.35e-06

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 47.71  E-value: 1.35e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  40 RIHGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLEnsSRQSYYTCKAvcsigrdhpdvHRYSVETVQWYPhDTGMFTSSS 119
Cdd:cd00200 216 RGHENGVNSVAFSP-DGYLLASGSEDGTIRVWDLR--TGECVQTLSG-----------HTNSVTSLAWSP-DGKRLASGS 280


                ....*....
gi 55956773 120 FDKTLKVWD 128
Cdd:cd00200 281 ADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
42-131 8.92e-06

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 45.29  E-value: 8.92e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 55956773  42 HGGGINTLDIEPvEGRYMLSGGSDGVIVLYDLENSSRQSYYTckavcsigrdhpdVHRYSVETVQWYPhDTGMFTSSSFD 121
Cdd:COG2319 329 HTGAVRSVAFSP-DGKTLASGSDDGTVRLWDLATGELLRTLT-------------GHTGAVTSVAFSP-DGRTLASGSAD 393

                        90
                ....*....|
gi 55956773 122 KTLKVWDTNT 131
Cdd:COG2319 394 GTVRLWDLAT 403
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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