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Conserved domains on  [gi|71043706|ref|NP_001020816|]
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crossover junction endonuclease MUS81 [Rattus norvegicus]

Protein Classification

crossover junction endonuclease MUS81( domain architecture ID 15338741)

crossover junction endonuclease MUS81 interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 266-419 2.44e-74

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


:

Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 232.76  E-value: 2.44e-74
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 266 EYRVLLCVDIGETRGAGHRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSII 345
Cdd:cd20074   1 SYEVVLLVDNREVKGKKDRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIK 76

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 71043706 346 DGRFREQKFRLKRCGLGHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 419
Cdd:cd20074  77 DGRYHEQKFRLKRSGIKNVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 3.52e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


:

Pssm-ID: 411029  Cd Length: 94  Bit Score: 91.06  E-value: 3.52e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036   1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                        90
                ....*....|....*.
gi 71043706 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036  79 SLTEEGRELAEKLAEA 94
 
Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 266-419 2.44e-74

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 232.76  E-value: 2.44e-74
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 266 EYRVLLCVDIGETRGAGHRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSII 345
Cdd:cd20074   1 SYEVVLLVDNREVKGKKDRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIK 76

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 71043706 346 DGRFREQKFRLKRCGLGHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 419
Cdd:cd20074  77 DGRYHEQKFRLKRSGIKNVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
ERCC4 pfam02732
ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an ...
 273-415 4.28e-32

ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an essential component of a Holliday junction resolvase. EME1 interacts with MUS81 to form a DNA structure-specific endonuclease.


Pssm-ID: 426945 [Multi-domain]  Cd Length: 139  Bit Score: 120.23  E-value: 4.28e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706   273 VDIGEtrgagHRPEMLRE-LQRLRVPHTVRKLHVGDFVWVAQETRPRdperpGELVLDHIVERKRLDDLCSSIIDGRFRE 351
Cdd:pfam02732   1 VDTRE-----LRSSIPELlLEELGVEVVVETLPVGDYLWVPREYDLE-----LEVVLDVIVERKSLDDLVSSIIDGRLFE 70

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706   352 QKFRLKRcGLGHRIYLVEEHGSVQN------LSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 415
Cdd:pfam02732  71 QKSRLKR-GYKKPILLVEGLDLFSRklknkrRDINPNAIEGALASLQVDYGVRIIRTRSAEETAEWLASL 139
ERCC4 smart00891
ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, ...
 270-369 1.32e-29

ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, such as Rad1/Mus81/XPF endonucleases, and in ATP-dependent helicases. The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases.


Pssm-ID: 214888 [Multi-domain]  Cd Length: 98  Bit Score: 112.06  E-value: 1.32e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706    270 LLCVDIGETRGAGHRPeMLRELQ-RLRVPHTVRKLHVGDFVWVAQETRPRDPErpgELVLDHIVERKRLDDLCSSIIDGR 348
Cdd:smart00891   1 EIIVDSRELRSALEAP-IPRSLRwKRGVKVEYDRLEAGDFVLVARDKQSLLPH---VNSLNELVERKSLTDLVASIPDGR 76

                           90       100
                   ....*....|....*....|.
gi 71043706    349 FREQKFRLKRCGLGHRIYLVE 369
Cdd:smart00891  77 LFEQVRRLQQIAYPSPQLLVE 97
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 3.52e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


Pssm-ID: 411029  Cd Length: 94  Bit Score: 91.06  E-value: 3.52e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036   1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                        90
                ....*....|....*.
gi 71043706 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036  79 SLTEEGRELAEKLAEA 94
MUS81 COG1948
ERCC4-type crossover junction endonuclease [Replication, recombination and repair];
284-506 4.82e-07

ERCC4-type crossover junction endonuclease [Replication, recombination and repair];


Pssm-ID: 441551 [Multi-domain]  Cd Length: 214  Bit Score: 50.56  E-value: 4.82e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 284 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKRCgLG 362
Cdd:COG1948  12 NSGVPRLLSRLGVEVRVKTLEVGDYV-----------------VSDRVaVERKTVRDFVNSLIDGRLFEQASRLAEA-YE 73

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 363 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL-ERLYQGHTLHSRPwgtpgdaes 441
Cdd:COG1948  74 RPVLIIEGDLLYEERNIHPNAIRGALASLALDFGIPVLPTRDAEDTAELLVTLARREqEEEKREVSLHGKK--------- 144

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 71043706 442 eakpstnplcslltfsdfnaeavknKAQSVREvfaRQLMQVRGLSG---EKAAALVDRYSTPASLLAA 506
Cdd:COG1948 145 -------------------------KPKTLRE---QQLYVVESLPGigpKLARRLLEHFGSVEAVFNA 184
PRK13766 PRK13766
Hef nuclease; Provisional
 284-358 5.81e-04

Hef nuclease; Provisional


Pssm-ID: 237496 [Multi-domain]  Cd Length: 773  Bit Score: 42.94  E-value: 5.81e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 71043706  284 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKR 358
Cdd:PRK13766 573 RSNVARHLKRLGAEVELKTLEVGDYV-----------------VSDRVaVERKTAEDFVDSIIDRRLFEQVKDLKR 631
 
Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 266-419 2.44e-74

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 232.76  E-value: 2.44e-74
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 266 EYRVLLCVDIGETRGAGHRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSII 345
Cdd:cd20074   1 SYEVVLLVDNREVKGKKDRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIK 76

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 71043706 346 DGRFREQKFRLKRCGLGHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 419
Cdd:cd20074  77 DGRYHEQKFRLKRSGIKNVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
XPF_nuclease-like cd19940
nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap ...
 270-417 7.83e-35

nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap endonuclease that act upon 3'-flap structures and involved in DNA repair pathways that are necessary for the removal of UV-light-induced DNA lesions and cross-links between DNA strands. Family members exist either as heterodimers or as homodimers in their functionally competent states which consist of a catalytic and a noncatalytic subunit. The catalytic subunits have a DX(n)RKX(3)D motif. This motif is required for metal-dependent endonuclease activity but not for DNA junction binding. The equivalent regions of the noncatalytic subunits (ERCC1, EME1, and FAAP24) have diverged. The noncatalytic subunits have roles such as binding ssDNA or an ability to target the endonuclease to defined DNA structures or sites of DNA damage.


Pssm-ID: 410849 [Multi-domain]  Cd Length: 126  Bit Score: 127.50  E-value: 7.83e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 270 LLCVDIGETRgaghrPEMLRELQRLRVPHTVRKLHVGDFVWVAqetrprdperpgelvlDHIVERKRLDDLCSSIIDGRF 349
Cdd:cd19940   1 SIVVDPRERR-----SELLSELQRLGVQVEFEDLAVGDYVLSN----------------RTCVERKSLSDLVSSINKGRL 59

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 71043706 350 REQKFRLKRCGLgHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTK 417
Cdd:cd19940  60 REQLQRLTRKFE-RRVLLVEKDRSKFRSMVSSVQALSALTKLQLLTGIRLLIVASPKETADLLEELTQ 126
XPF_ERCC4_MUS81-like cd22367
XPF family DNA repair endonuclease; (Xeroderma Pigmentosum group F) DNA repair gene homologs ...
 271-415 1.28e-34

XPF family DNA repair endonuclease; (Xeroderma Pigmentosum group F) DNA repair gene homologs are members of the XPF/Rad1/Mus81-dependent nuclease family which specifically cleave branched structures generated during DNA repair, replication, and recombination, and they are essential for maintaining genome stability. They belong to a wider superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411771 [Multi-domain]  Cd Length: 123  Bit Score: 126.61  E-value: 1.28e-34
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 271 LCVDIGETRGaghrpEMLRELQRLRVPHTVRKLHVGDFVWVAqetrprdperpgelvlDHIVERKRLDDLCSSIIDGRFR 350
Cdd:cd22367   1 IVVDSRERRS-----GLPELLRKLGVRVEVRTLEVGDYILSA----------------DIIVERKTVSDLISSIIDGRLF 59

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 71043706 351 EQKFRLKRCGLgHRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 415
Cdd:cd22367  60 EQAERLKRSYE-RPILLIEGDPDKARRLVRPAALGAAISSLLVIGGLLVLRTPNFETTALLLSLL 123
ERCC4 pfam02732
ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an ...
 273-415 4.28e-32

ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an essential component of a Holliday junction resolvase. EME1 interacts with MUS81 to form a DNA structure-specific endonuclease.


Pssm-ID: 426945 [Multi-domain]  Cd Length: 139  Bit Score: 120.23  E-value: 4.28e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706   273 VDIGEtrgagHRPEMLRE-LQRLRVPHTVRKLHVGDFVWVAQETRPRdperpGELVLDHIVERKRLDDLCSSIIDGRFRE 351
Cdd:pfam02732   1 VDTRE-----LRSSIPELlLEELGVEVVVETLPVGDYLWVPREYDLE-----LEVVLDVIVERKSLDDLVSSIIDGRLFE 70

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706   352 QKFRLKRcGLGHRIYLVEEHGSVQN------LSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 415
Cdd:pfam02732  71 QKSRLKR-GYKKPILLVEGLDLFSRklknkrRDINPNAIEGALASLQVDYGVRIIRTRSAEETAEWLASL 139
ERCC4 smart00891
ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, ...
 270-369 1.32e-29

ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, such as Rad1/Mus81/XPF endonucleases, and in ATP-dependent helicases. The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases.


Pssm-ID: 214888 [Multi-domain]  Cd Length: 98  Bit Score: 112.06  E-value: 1.32e-29
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706    270 LLCVDIGETRGAGHRPeMLRELQ-RLRVPHTVRKLHVGDFVWVAQETRPRDPErpgELVLDHIVERKRLDDLCSSIIDGR 348
Cdd:smart00891   1 EIIVDSRELRSALEAP-IPRSLRwKRGVKVEYDRLEAGDFVLVARDKQSLLPH---VNSLNELVERKSLTDLVASIPDGR 76

                           90       100
                   ....*....|....*....|.
gi 71043706    349 FREQKFRLKRCGLGHRIYLVE 369
Cdd:smart00891  77 LFEQVRRLQQIAYPSPQLLVE 97
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 3.52e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


Pssm-ID: 411029  Cd Length: 94  Bit Score: 91.06  E-value: 3.52e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036   1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                        90
                ....*....|....*.
gi 71043706 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036  79 SLTEEGRELAEKLAEA 94
XPF_nuclease_XPF_arch cd20075
nuclease domain of XPF found in archaea; XPF, also called DNA excision repair protein ERCC-4, ...
 284-358 1.55e-08

nuclease domain of XPF found in archaea; XPF, also called DNA excision repair protein ERCC-4, or DNA repair protein complementing XP-F cells, or Xeroderma pigmentosum group F-complementing protein, is a 3'-flap repair endonuclease that cleaves 5' of ds/ssDNA interfaces in 3' flap structures, although it also cuts bubble, Y-DNA structures and mobile and immobile Holliday junctions. XPF cuts preferentially after pyrimidines, may continue to progressively cleave substrate upstream of the initial cleavage, at least in vitro. It may be involved in nucleotide excision repair. The nuclease domains of the catalytic subunits XPF have the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity but not for DNA junction binding. XPF-ERRC1 and its yeast homolog Rad1-Rad10 play key roles in the excision of DNA lesions and are required for certain types of homologous recombination events and for the repair of DNA cross-links.


Pssm-ID: 410851 [Multi-domain]  Cd Length: 127  Bit Score: 53.16  E-value: 1.55e-08
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 71043706 284 RPEMLRELQRLRVPHTVRKLHVGDFVwVAQETrprdperpgelvldhIVERKRLDDLCSSIIDGRFREQKFRLKR 358
Cdd:cd20075  10 NSGVVRELKELGVEVEFKQLEVGDYI-VSDRV---------------AIERKTVDDFVSSIIDGRLFDQAKRLKE 68
MUS81 COG1948
ERCC4-type crossover junction endonuclease [Replication, recombination and repair];
284-506 4.82e-07

ERCC4-type crossover junction endonuclease [Replication, recombination and repair];


Pssm-ID: 441551 [Multi-domain]  Cd Length: 214  Bit Score: 50.56  E-value: 4.82e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 284 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKRCgLG 362
Cdd:COG1948  12 NSGVPRLLSRLGVEVRVKTLEVGDYV-----------------VSDRVaVERKTVRDFVNSLIDGRLFEQASRLAEA-YE 73

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71043706 363 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL-ERLYQGHTLHSRPwgtpgdaes 441
Cdd:COG1948  74 RPVLIIEGDLLYEERNIHPNAIRGALASLALDFGIPVLPTRDAEDTAELLVTLARREqEEEKREVSLHGKK--------- 144

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 71043706 442 eakpstnplcslltfsdfnaeavknKAQSVREvfaRQLMQVRGLSG---EKAAALVDRYSTPASLLAA 506
Cdd:COG1948 145 -------------------------KPKTLRE---QQLYVVESLPGigpKLARRLLEHFGSVEAVFNA 184
PRK13766 PRK13766
Hef nuclease; Provisional
 284-358 5.81e-04

Hef nuclease; Provisional


Pssm-ID: 237496 [Multi-domain]  Cd Length: 773  Bit Score: 42.94  E-value: 5.81e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 71043706  284 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKR 358
Cdd:PRK13766 573 RSNVARHLKRLGAEVELKTLEVGDYV-----------------VSDRVaVERKTAEDFVDSIIDRRLFEQVKDLKR 631
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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