KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 93277110     7 LLTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSLD 48
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*.
gi 93277110   257 YVARHRRCHTGEKPYKCNECGKTFSQ 282
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 93277110     7 LLTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSLD 48
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 93277110   8 LTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSL 47
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*
gi 93277110   314 LQIHRRIHTGEKPYKCNECGKTFSQ 338
Cdd:pfam13465   2 LKRHMRTHTGEKPYKCPECGKSFKS 26

KRAB box; The KRAB domain (or Kruppel-associated box) is present in about a third of zinc finger proteins containing C2H2 fingers. The KRAB domain is found to be involved in protein-protein interactions. The KRAB domain is generally encoded by two exons. The regions coded by the two exons are known as KRAB-A and KRAB-B. The A box plays an important role in repression by binding to corepressors, while the B box is thought to enhance this repression brought about by the A box. KRAB-containing proteins are thought to have critical functions in cell proliferation and differentiation, apoptosis and neoplastic transformation.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 93277110     7 LLTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSLD 48
Cdd:pfam01352   1 SVTFEDVAVDFTQEEWALLDPAQRNLYRDVMLENYRNLVSLG 42

KRAB (Kruppel-associated box) domain -A box; The KRAB domain is a transcription repression module, found in a subgroup of the zinc finger proteins (ZFPs) of the C2H2 family, KRAB-ZFPs. KRAB-ZFPs comprise the largest group of transcriptional regulators in mammals, and are only found in tetrapods. These proteins have been shown to play important roles in cell differentiation and organ development, and in regulating viral replication and transcription. A KRAB domain may consist of an A-box, or of an A-box plus either a B-box, a divergent B-box (b), or a C-box. Only the A-box is included in this model. The A-box is needed for repression, the B- and C- boxes are not. KRAB-ZFPs have one or two KRAB domains at their amino-terminal end, and multiple C2H2 zinc finger motifs at their C-termini. Some KRAB-ZFPs also contain a SCAN domain which mediates homo- and hetero-oligomerization. The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta , KRAB-A interacting protein , and tripartite motif protein 28). The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|
gi 93277110   8 LTFRDVAIEFSQEEWKCLDPAQRTLYRDVMLENYRNLVSL 47
Cdd:cd07765   1 VTFEDVAVYFSQEEWELLDPAQRDLYRDVMLENYENLVSL 40

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277110 241 KQYKCDICGKVFNSKRYVARHRRCHTGEKPYKCN--ECGKTFSQTYYLTCHRRLHTGEKPYKCE-ECDKAFHFKSKLQIH 317
Cdd:COG5048  32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSysGCDKSFSRPLELSRHLRTHHNNPSDLNSkSLPLSNSKASSSSLS 111

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277110 318 RRIHTGEKPYKCNECGKTFSQKSYLTCHRRLHTGEKPYKCNEC----------------------GKTFSRKSHFTCHHR 375
Cdd:COG5048 112 SSSSNSNDNNLLSSHSLPPSSRDPQLPDLLSISNLRNNPLPGNnsssvntpqsnslhpplpanslSKDPSSNLSLLISSN 191

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277110 376 VHTGEKPYKCNECSKTFSHKSSLTYHRRLHTEEKPYKCNECGKTFNQQLTLNIcRLHSGEKPYKCEECDKAYSFKSNLEI 455
Cdd:COG5048 192 VSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSLPLTTNSQLSPKSLLSQS-PSSLSSSDSSSSASESPRSSLPTASS 270

                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 93277110 456 HQKIHTE---------ENPYKCNECGKTFSRTSSLTYH--HRLHTGQ--KPYKC--EDCDEAFSFKSNLERHRRIYTG 518
Cdd:COG5048 271 QSSSPNEsdsssekgfSLPIKSKQCNISFSRSSPLTRHlrSVNHSGEslKPFSCpySLCGKLFSRNDALKRHILLHTS 348

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277110 434 GEKPYKCE--ECDKAYSFKSNLEIHqKIHTEENPykcnecgkTFSRTSSLTYHHRLHTGQKPYKCEDCDEAFSFKSNLER 511
Cdd:COG5189 346 DGKPYKCPveGCNKKYKNQNGLKYH-MLHGHQNQ--------KLHENPSPEKMNIFSAKDKPYRCEVCDKRYKNLNGLKY 416

                ....*.
gi 93277110 512 HRRIYT 517
Cdd:COG5189 417 HRKHSH 422

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*
gi 93277110   314 LQIHRRIHTGEKPYKCNECGKTFSQ 338
Cdd:pfam13465   2 LKRHMRTHTGEKPYKCPECGKSFKS 26

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93277110 281 SQTYYLTCHRRLHT-GEKPYKCE--ECDKAFHFKSKLQIHRRihtgekpykcnecgktfsqksYLTCHRRLHTGEKPYKC 357
Cdd:COG5189 331 GERNIDTPSRMLKVkDGKPYKCPveGCNKKYKNQNGLKYHML---------------------HGHQNQKLHENPSPEKM 389

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 93277110 358 NecgkTFSRKshftchhrvhtgEKPYKCNECSKTFSHKSSLTYHR 402
Cdd:COG5189 390 N----IFSAK------------DKPYRCEVCDKRYKNLNGLKYHR 418