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Conserved domains on  [gi|108796058|ref|NP_001035827|]
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ral guanine nucleotide dissociation stimulator isoform 2 [Homo sapiens]

Protein Classification

RasGEF and RA_RalGDS domain-containing protein( domain architecture ID 13898960)

protein containing domains REM, PRK07003, RasGEF, and RA_RalGDS

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 327-589 3.01e-90

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


:

Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 284.92  E-value: 3.01e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 327 LLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKgkEHLAPTIRATVTQFNSVANCVITTCLGnrSTKAPDRAR 406
Cdd:cd00155    1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKN--IHLSPNLERFIERFNNLSNWVASEILL--CTNPKKRAR 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 407 VVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFa 486
Cdd:cd00155   77 LLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGPNPP- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 487 tlemnpkraqkrpketgiiqgTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSacNNYSIAPDE 566
Cdd:cd00155  156 ---------------------CVPFLGVYLKDLTFLHEGNPDFLEGNLVNFEKRRKIAEILREIRQLQS--NSYELNRDE 212

                        250       260
                 ....*....|....*....|....*
gi 108796058 567 QFGAWFRAVER--LSETESYNLSCE 589
Cdd:cd00155  213 DILAFLWKLLEliLNEDELYELSLE 237
RA_RalGDS cd17209
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) ...
 743-828 1.82e-47

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) and similar proteins; RalGDS, also termed Ral guanine nucleotide exchange factor (RalGEF), is a guanine exchange factor (GEF) for the Ral family of small GTPases. It is the prototype of RalGDS family proteins that are involved in Ras and Ral signaling pathways as downstream effector proteins. RalGDS stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. It interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. Moreover, RalGDS functions as a novel interacting partner for Rab7-interacting lysosomal protein (RILP), a key regulator for late endosomal/lysosomal trafficking. RILP suppresses invasion of breast cancer cells by inhibiting the GEF activity for RalA of RalGDS. RalGDS also plays a vital role in the regulation of Ral-dependent Weibel-Palade bodies (WPB) exocytosis from endothelial cells. In addition, RalGDS couples growth factor signaling to Akt activation by promoting PDK1-induced Akt phosphorylation. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


:

Pssm-ID: 340729  Cd Length: 86  Bit Score: 163.20  E-value: 1.82e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYD 822
Cdd:cd17209    1 DCCIIRVSLDVDNGNMYKSILVTSQDKTPVVIRKAMAKHNLDEEEPEDYELLQILSEDRELKIPDNANVFYAMNSTANYD 80


                 ....*.
gi 108796058 823 FVLKKR 828
Cdd:cd17209   81 FVLKKR 86
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 57-193 1.87e-34

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 127.84  E-value: 1.87e-34
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058    57 KVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKrygrcdaltassRYGCILPYS-DEDGGPQ 135
Cdd:smart00229   1 DGGLIKGGTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLY------------RYNAIPPESwVEEKVNP 68

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 108796058   136 DQLKNAISSILGTWLDQYSEDFCQPP-DFPCLKQLvAYVQLNMPGSDLERRAHLLLAQL 193
Cdd:smart00229  69 RRVKNRVLNILRTWVENYWEDFEDDPkLISFLLEF-LELVDDEKYPGLVTSLLNLLRRL 126
GGN super family cl37895
Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the ...
 192-303 1.18e-07

Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the maturation of sperm and is expressed virtually only in the testis. It is found to be associated with the intracellular membrane, binds with GGNBP1 and may be involved in vesicular trafficking.


The actual alignment was detected with superfamily member pfam15685:

Pssm-ID: 434857 [Multi-domain]  Cd Length: 668  Bit Score: 55.54  E-value: 1.18e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  192 QLEHSEPIEAEPEALSPVPAlkPTPELELaltparaPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELqqapEPAVGLE 271
Cdd:pfam15685 439 ALLPRPPPPTPPATPPPVPP--PIPQLPA-------LQPMPLAAARPPTPRPCPGHGESALAPAPTAPL----PPALAAD 505

                          90       100       110
                  ....*....|....*....|....*....|..
gi 108796058  272 SAPAPALELEPAPEQDPAPSQTLELEPAPAPV 303
Cdd:pfam15685 506 QAPAPALAAAPAPSPAPAPATADPLPPAPAPI 537
 
Name Accession Description Interval E-value
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 327-589 3.01e-90

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 284.92  E-value: 3.01e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 327 LLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKgkEHLAPTIRATVTQFNSVANCVITTCLGnrSTKAPDRAR 406
Cdd:cd00155    1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKN--IHLSPNLERFIERFNNLSNWVASEILL--CTNPKKRAR 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 407 VVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFa 486
Cdd:cd00155   77 LLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGPNPP- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 487 tlemnpkraqkrpketgiiqgTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSacNNYSIAPDE 566
Cdd:cd00155  156 ---------------------CVPFLGVYLKDLTFLHEGNPDFLEGNLVNFEKRRKIAEILREIRQLQS--NSYELNRDE 212

                        250       260
                 ....*....|....*....|....*
gi 108796058 567 QFGAWFRAVER--LSETESYNLSCE 589
Cdd:cd00155  213 DILAFLWKLLEliLNEDELYELSLE 237
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
327-594 1.48e-87

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 277.97  E-value: 1.48e-87
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   327 LLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLapTIRATVTQFNSVANCVITTCLGnrSTKAPDRAR 406
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPL--NLEAFIRRFNEVSNWVATEILK--QTTPKDRAE 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   407 VVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTskfa 486
Cdd:smart00147  77 LLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCNL---- 152

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   487 tlemnpkraqkrpketgiiQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSAcnNYSIAPDE 566
Cdd:smart00147 153 -------------------PPCIPFLGVLLKDLTFIDEGNPDFLENGLVNFEKRRQIAEILREIRQLQSQ--PYNLRPNR 211

                          250       260       270
                   ....*....|....*....|....*....|.
gi 108796058   567 Q-FGAWFRAVERL--SETESYNLSCELEPPS 594
Cdd:smart00147 212 SdIQSLLQQLLDHldEEEELYQLSLKIEPRV 242
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 335-541 4.22e-71

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 231.71  E-value: 4.22e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  335 VAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKehlAPTIRATVTQFNSVANCVITTCLgnRSTKAPDRARVVEHWIEV 414
Cdd:pfam00617   2 LARQLTLIEFELFRKIKPRELLGSAWSKKDKKEN---SPNIEAMIARFNKLSNWVASEIL--SEEDLKKRAKVIKKFIKI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  415 ARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTskfatlemnpkr 494
Cdd:pfam00617  77 AEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASP------------ 144

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 108796058  495 aqkrpketgiiqGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRR 541
Cdd:pfam00617 145 ------------PCIPFLGLYLTDLTFIEEGNPDFLEGGLINFEKRR 179
RA_RalGDS cd17209
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) ...
 743-828 1.82e-47

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) and similar proteins; RalGDS, also termed Ral guanine nucleotide exchange factor (RalGEF), is a guanine exchange factor (GEF) for the Ral family of small GTPases. It is the prototype of RalGDS family proteins that are involved in Ras and Ral signaling pathways as downstream effector proteins. RalGDS stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. It interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. Moreover, RalGDS functions as a novel interacting partner for Rab7-interacting lysosomal protein (RILP), a key regulator for late endosomal/lysosomal trafficking. RILP suppresses invasion of breast cancer cells by inhibiting the GEF activity for RalA of RalGDS. RalGDS also plays a vital role in the regulation of Ral-dependent Weibel-Palade bodies (WPB) exocytosis from endothelial cells. In addition, RalGDS couples growth factor signaling to Akt activation by promoting PDK1-induced Akt phosphorylation. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340729  Cd Length: 86  Bit Score: 163.20  E-value: 1.82e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYD 822
Cdd:cd17209    1 DCCIIRVSLDVDNGNMYKSILVTSQDKTPVVIRKAMAKHNLDEEEPEDYELLQILSEDRELKIPDNANVFYAMNSTANYD 80


                 ....*.
gi 108796058 823 FVLKKR 828
Cdd:cd17209   81 FVLKKR 86
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 57-193 1.87e-34

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 127.84  E-value: 1.87e-34
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058    57 KVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKrygrcdaltassRYGCILPYS-DEDGGPQ 135
Cdd:smart00229   1 DGGLIKGGTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLY------------RYNAIPPESwVEEKVNP 68

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 108796058   136 DQLKNAISSILGTWLDQYSEDFCQPP-DFPCLKQLvAYVQLNMPGSDLERRAHLLLAQL 193
Cdd:smart00229  69 RRVKNRVLNILRTWVENYWEDFEDDPkLISFLLEF-LELVDDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 65-195 1.04e-28

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 111.35  E-value: 1.04e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  65 TLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDAltassrygCILPYSDEDGGPQDQLKNAISS 144
Cdd:cd06224    1 TLEALIEHLTSTFDMPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPP--------ENLEYNDWDKKKSKPIRLRVLN 72

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 108796058 145 ILGTWLDQYSEDFCqpPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEH 195
Cdd:cd06224   73 VLRTWVENYPYDFF--DDEELLELLEEFLNRLVQEGALLQELKKLLRKLLK 121
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 60-170 1.87e-25

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 101.23  E-value: 1.87e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   60 TVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYG-RCDALTASSRYgcilpysdEDGGPQDQL 138
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLDDSFLSTFLLTYRSFTTPAELLELLIERYNiPPPLDLSSDSY--------WISKKTLPI 72

                          90       100       110
                  ....*....|....*....|....*....|..
gi 108796058  139 KNAISSILGTWLDQYSEDFCQPPdfPCLKQLV 170
Cdd:pfam00618  73 RIRVLSVLRHWVENYFSDFNDDP--VLLSRLE 102
RA smart00314
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 743-828 6.38e-13

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Kalhammer et al. have shown that not all RA domains bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase. Predicted RA domains in PLC210 and nore1 found to bind RasGTP. Included outliers (Grb7, Grb14, adenylyl cyclases etc.)


Pssm-ID: 214612  Cd Length: 90  Bit Score: 65.01  E-value: 6.38e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNlEEEEPEDYELLQILSDDRKLKIPENANVFYAMN----ST 818
Cdd:smart00314   1 DTFVLRVYVDDLPGGTYKTLRVSSRTTARDVIQQLLEKFH-LTDDPEEYVLVEVLPDGKERVLPDDENPLQLQKlwprRG 79

                           90
                   ....*....|
gi 108796058   819 ANYDFVLKKR 828
Cdd:smart00314  80 PNLRFVLRKR 89
RA pfam00788
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 743-828 1.16e-09

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Recent evidence (not yet in MEDLINE) shows that some RA domains do NOT bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase.


Pssm-ID: 425871  Cd Length: 93  Bit Score: 55.80  E-value: 1.16e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  743 DCCIIRVSLDVDN-GNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMN----S 817
Cdd:pfam00788   1 DDGVLKVYTEDGKpGTTYKTILVSSSTTAEEVIEALLEKFGLEDDPRDYVLVEVLERGGGERRLPDDECPLQIQLqwprD 80

                          90
                  ....*....|.
gi 108796058  818 TANYDFVLKKR 828
Cdd:pfam00788  81 ASDSRFLLRKR 91
GGN pfam15685
Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the ...
 192-303 1.18e-07

Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the maturation of sperm and is expressed virtually only in the testis. It is found to be associated with the intracellular membrane, binds with GGNBP1 and may be involved in vesicular trafficking.


Pssm-ID: 434857 [Multi-domain]  Cd Length: 668  Bit Score: 55.54  E-value: 1.18e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  192 QLEHSEPIEAEPEALSPVPAlkPTPELELaltparaPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELqqapEPAVGLE 271
Cdd:pfam15685 439 ALLPRPPPPTPPATPPPVPP--PIPQLPA-------LQPMPLAAARPPTPRPCPGHGESALAPAPTAPL----PPALAAD 505

                          90       100       110
                  ....*....|....*....|....*....|..
gi 108796058  272 SAPAPALELEPAPEQDPAPSQTLELEPAPAPV 303
Cdd:pfam15685 506 QAPAPALAAAPAPSPAPAPATADPLPPAPAPI 537
PRK04654 PRK04654
sec-independent translocase; Provisional
 247-324 1.29e-04

sec-independent translocase; Provisional


Pssm-ID: 135173 [Multi-domain]  Cd Length: 214  Bit Score: 44.03  E-value: 1.29e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 247 GSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQ-TLELEPAPAPVPSLQP----SWPSPVVAENGLS 321
Cdd:PRK04654 130 GAEPGAGQAHTPVPAPAPVIAQAQPIAPAPHQTLVPAPHDTIVPAPhAAHLPSAPATPVSVAPvdagTSASPTPSEPTKI 209


                 ...
gi 108796058 322 EEK 324
Cdd:PRK04654 210 QEK 212
COG5373 COG5373
Uncharacterized membrane protein [Function unknown];
248-321 2.45e-03

Uncharacterized membrane protein [Function unknown];


Pssm-ID: 444140 [Multi-domain]  Cd Length: 854  Bit Score: 41.52  E-value: 2.45e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 108796058 248 SELE---VAPAPAPELQQAPEP-AVGLESAPAPALELEPAPEQDPAPSQTLElepAPAPVPSLQPSWPSPVVAENGLS 321
Cdd:COG5373   31 EELEaelAEAAEAASAPAEPEPeAAAAATAAAPEAAPAPVPEAPAAPPAAAE---APAPAAAAPPAEAEPAAAPAAAS 105
 
Name Accession Description Interval E-value
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 327-589 3.01e-90

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 284.92  E-value: 3.01e-90
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 327 LLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKgkEHLAPTIRATVTQFNSVANCVITTCLGnrSTKAPDRAR 406
Cdd:cd00155    1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKN--IHLSPNLERFIERFNNLSNWVASEILL--CTNPKKRAR 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 407 VVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFa 486
Cdd:cd00155   77 LLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGPNPP- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 487 tlemnpkraqkrpketgiiqgTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSacNNYSIAPDE 566
Cdd:cd00155  156 ---------------------CVPFLGVYLKDLTFLHEGNPDFLEGNLVNFEKRRKIAEILREIRQLQS--NSYELNRDE 212

                        250       260
                 ....*....|....*....|....*
gi 108796058 567 QFGAWFRAVER--LSETESYNLSCE 589
Cdd:cd00155  213 DILAFLWKLLEliLNEDELYELSLE 237
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
327-594 1.48e-87

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 277.97  E-value: 1.48e-87
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   327 LLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLapTIRATVTQFNSVANCVITTCLGnrSTKAPDRAR 406
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPL--NLEAFIRRFNEVSNWVATEILK--QTTPKDRAE 76

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   407 VVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTskfa 486
Cdd:smart00147  77 LLSKFIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCNL---- 152

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   487 tlemnpkraqkrpketgiiQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSAcnNYSIAPDE 566
Cdd:smart00147 153 -------------------PPCIPFLGVLLKDLTFIDEGNPDFLENGLVNFEKRRQIAEILREIRQLQSQ--PYNLRPNR 211

                          250       260       270
                   ....*....|....*....|....*....|.
gi 108796058   567 Q-FGAWFRAVERL--SETESYNLSCELEPPS 594
Cdd:smart00147 212 SdIQSLLQQLLDHldEEEELYQLSLKIEPRV 242
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 335-541 4.22e-71

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 231.71  E-value: 4.22e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  335 VAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKehlAPTIRATVTQFNSVANCVITTCLgnRSTKAPDRARVVEHWIEV 414
Cdd:pfam00617   2 LARQLTLIEFELFRKIKPRELLGSAWSKKDKKEN---SPNIEAMIARFNKLSNWVASEIL--SEEDLKKRAKVIKKFIKI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  415 ARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTskfatlemnpkr 494
Cdd:pfam00617  77 AEHCRELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASP------------ 144

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 108796058  495 aqkrpketgiiqGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRR 541
Cdd:pfam00617 145 ------------PCIPFLGLYLTDLTFIEEGNPDFLEGGLINFEKRR 179
RA_RalGDS cd17209
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) ...
 743-828 1.82e-47

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator (RalGDS) and similar proteins; RalGDS, also termed Ral guanine nucleotide exchange factor (RalGEF), is a guanine exchange factor (GEF) for the Ral family of small GTPases. It is the prototype of RalGDS family proteins that are involved in Ras and Ral signaling pathways as downstream effector proteins. RalGDS stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. It interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap. Moreover, RalGDS functions as a novel interacting partner for Rab7-interacting lysosomal protein (RILP), a key regulator for late endosomal/lysosomal trafficking. RILP suppresses invasion of breast cancer cells by inhibiting the GEF activity for RalA of RalGDS. RalGDS also plays a vital role in the regulation of Ral-dependent Weibel-Palade bodies (WPB) exocytosis from endothelial cells. In addition, RalGDS couples growth factor signaling to Akt activation by promoting PDK1-induced Akt phosphorylation. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340729  Cd Length: 86  Bit Score: 163.20  E-value: 1.82e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYD 822
Cdd:cd17209    1 DCCIIRVSLDVDNGNMYKSILVTSQDKTPVVIRKAMAKHNLDEEEPEDYELLQILSEDRELKIPDNANVFYAMNSTANYD 80


                 ....*.
gi 108796058 823 FVLKKR 828
Cdd:cd17209   81 FVLKKR 86
RA_RGL cd17210
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 1 ...
 743-828 2.27e-36

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 1 (RalGDS-like 1) and similar proteins; RalGDS-like 1 (RGL) is a Ral-specific guanine nucleotide exchange factor that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL has been identified as a possible effector protein of Ras. It also regulates c-fos promoter and the GDP/GTP exchange of Ral. Members in this family have similar structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340730  Cd Length: 87  Bit Score: 132.03  E-value: 2.27e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYD 822
Cdd:cd17210    2 DTCIIRVSVEDNNGNMYKSIMLTSQDKTPAVIQRAMSKHNLESDPAEDYELVQVISEDRELVIPDNANVFYAMNSSVNFD 81


                 ....*.
gi 108796058 823 FVLKKR 828
Cdd:cd17210   82 FILRKK 87
RA_RalGDS_like cd00153
Ras-associating (RA) domain of RalGDS family; The RalGDS family RA domains can interact with ...
 743-828 1.06e-35

Ras-associating (RA) domain of RalGDS family; The RalGDS family RA domains can interact with activated Ras and may function as effectors for other Ras family. Ras proteins are small GTPases that are involved in cellular signal transduction. The RA domain has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub); Ub is a protein modifier in eukaryotes and is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. The RalGDS family includes RalGDS, RGL, RGL2/Rlf and RGL3. All family members have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal RA domain. The RA domain mediates the GTP-dependent interaction with Ras and Ras-related proteins.


Pssm-ID: 340449  Cd Length: 88  Bit Score: 130.00  E-value: 1.06e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLD--VDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTAN 820
Cdd:cd00153    1 DSRIIRVSLEdgSEDGNLYKSILLTNQDRTPSVIRRALEKHNLEDEDPDDFSLVQILPDDKELVIPDNANVFYAMNSSAN 80


                 ....*...
gi 108796058 821 YDFVLKKR 828
Cdd:cd00153   81 LNFILRKK 88
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 57-193 1.87e-34

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 127.84  E-value: 1.87e-34
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058    57 KVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKrygrcdaltassRYGCILPYS-DEDGGPQ 135
Cdd:smart00229   1 DGGLIKGGTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLY------------RYNAIPPESwVEEKVNP 68

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 108796058   136 DQLKNAISSILGTWLDQYSEDFCQPP-DFPCLKQLvAYVQLNMPGSDLERRAHLLLAQL 193
Cdd:smart00229  69 RRVKNRVLNILRTWVENYWEDFEDDPkLISFLLEF-LELVDDEKYPGLVTSLLNLLRRL 126
RA_RGL3 cd17212
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 3 ...
 746-828 1.50e-29

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 3 (RalGDS-like 3) and similar proteins; RalGDS-like 3 (RGL3), also termed Ras pathway modulator (RPM), interacts in a GTP- and effector loop-dependent manner with Rit and Ras. As a novel potential effector of both p21 Ras and M-Ras, RGL3 negatively regulates Elk-1-dependent gene induction downstream of p21 Ras or mitogen activated protein/extracellular signal regulated kinase Kinase 1 (MEKK1). It also functions as a potential binding partner for Rap-family small G-proteins and profilin II. RGL3 belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier (Ubiquitination) in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair.


Pssm-ID: 340732  Cd Length: 87  Bit Score: 112.34  E-value: 1.50e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 746 IIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVL 825
Cdd:cd17212    5 VIRVSIDNDHGNLYRSILLTSQDKAPSVVQRALQKHNVPQPWARDYQLFQVLPGDRELLIPDNANVFYAMSPAAPGDFML 84


                 ...
gi 108796058 826 KKR 828
Cdd:cd17212   85 RRK 87
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 65-195 1.04e-28

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 111.35  E-value: 1.04e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  65 TLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDAltassrygCILPYSDEDGGPQDQLKNAISS 144
Cdd:cd06224    1 TLEALIEHLTSTFDMPDPSFVSTFLLTYRSFTTPTELLEKLIERYEIAPP--------ENLEYNDWDKKKSKPIRLRVLN 72

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 108796058 145 ILGTWLDQYSEDFCqpPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEH 195
Cdd:cd06224   73 VLRTWVENYPYDFF--DDEELLELLEEFLNRLVQEGALLQELKKLLRKLLK 121
RA_RGL2 cd17211
Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 ...
 743-828 1.53e-27

Ras-associating (RA) domain found in Ral guanine nucleotide dissociation stimulator-like 2 (RalGDS-like 2) and similar proteins; RalGDS-like 2 (RGL2), also termed RalGDS-like factor (RLF), or Ras-associated protein RAB2L, is a novel Ras and Rap 1A-associating protein that belongs to RalGDS family, whose members are involved in Ras and Ral signaling pathways as downstream effector proteins. RGL2 exhibits guanine nucleotide exchange activity towards the small GTPase Ral. Members in this family have similar domain structure: a central CDC25 homology domain with an upstream Ras Exchange motif (REM), and a C-terminal Ras-associating (RA) domain. The RA domain has the beta-grasp ubiquitin-like fold with low sequence similarity to ubiquitin; ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes including transcriptional regulation, cell cycle control, and DNA repair. The RA domain of RGL2 is phosphorylated by protein kinase A and the phosphorylation affects the ability of RGL2 to bind both Ras and Rap1.


Pssm-ID: 340731  Cd Length: 86  Bit Score: 106.45  E-value: 1.53e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNStANYD 822
Cdd:cd17211    2 DCRIIRVRMELHDGSVYKSILVTSQDKTPAVISRALEKHNQSSQAASPYELVQLLPEGKELTIPPTANVFYAMSS-ASLD 80


                 ....*.
gi 108796058 823 FVLKKR 828
Cdd:cd17211   81 FILRPR 86
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 60-170 1.87e-25

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 101.23  E-value: 1.87e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   60 TVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYG-RCDALTASSRYgcilpysdEDGGPQDQL 138
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLDDSFLSTFLLTYRSFTTPAELLELLIERYNiPPPLDLSSDSY--------WISKKTLPI 72

                          90       100       110
                  ....*....|....*....|....*....|..
gi 108796058  139 KNAISSILGTWLDQYSEDFCQPPdfPCLKQLV 170
Cdd:pfam00618  73 RIRVLSVLRHWVENYFSDFNDDP--VLLSRLE 102
RA smart00314
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 743-828 6.38e-13

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Kalhammer et al. have shown that not all RA domains bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase. Predicted RA domains in PLC210 and nore1 found to bind RasGTP. Included outliers (Grb7, Grb14, adenylyl cyclases etc.)


Pssm-ID: 214612  Cd Length: 90  Bit Score: 65.01  E-value: 6.38e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058   743 DCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNlEEEEPEDYELLQILSDDRKLKIPENANVFYAMN----ST 818
Cdd:smart00314   1 DTFVLRVYVDDLPGGTYKTLRVSSRTTARDVIQQLLEKFH-LTDDPEEYVLVEVLPDGKERVLPDDENPLQLQKlwprRG 79

                           90
                   ....*....|
gi 108796058   819 ANYDFVLKKR 828
Cdd:smart00314  80 PNLRFVLRKR 89
RA pfam00788
Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); ...
 743-828 1.16e-09

Ras association (RalGDS/AF-6) domain; RasGTP effectors (in cases of AF6, canoe and RalGDS); putative RasGTP effectors in other cases. Recent evidence (not yet in MEDLINE) shows that some RA domains do NOT bind RasGTP. Predicted structure similar to that determined, and that of the RasGTP-binding domain of Raf kinase.


Pssm-ID: 425871  Cd Length: 93  Bit Score: 55.80  E-value: 1.16e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  743 DCCIIRVSLDVDN-GNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMN----S 817
Cdd:pfam00788   1 DDGVLKVYTEDGKpGTTYKTILVSSSTTAEEVIEALLEKFGLEDDPRDYVLVEVLERGGGERRLPDDECPLQIQLqwprD 80

                          90
                  ....*....|.
gi 108796058  818 TANYDFVLKKR 828
Cdd:pfam00788  81 ASDSRFLLRKR 91
GGN pfam15685
Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the ...
 192-303 1.18e-07

Gametogenetin; GGN is a family of proteins largely found in mammals. It reacts with POG in the maturation of sperm and is expressed virtually only in the testis. It is found to be associated with the intracellular membrane, binds with GGNBP1 and may be involved in vesicular trafficking.


Pssm-ID: 434857 [Multi-domain]  Cd Length: 668  Bit Score: 55.54  E-value: 1.18e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  192 QLEHSEPIEAEPEALSPVPAlkPTPELELaltparaPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELqqapEPAVGLE 271
Cdd:pfam15685 439 ALLPRPPPPTPPATPPPVPP--PIPQLPA-------LQPMPLAAARPPTPRPCPGHGESALAPAPTAPL----PPALAAD 505

                          90       100       110
                  ....*....|....*....|....*....|..
gi 108796058  272 SAPAPALELEPAPEQDPAPSQTLELEPAPAPV 303
Cdd:pfam15685 506 QAPAPALAAAPAPSPAPAPATADPLPPAPAPI 537
PRK04654 PRK04654
sec-independent translocase; Provisional
 247-324 1.29e-04

sec-independent translocase; Provisional


Pssm-ID: 135173 [Multi-domain]  Cd Length: 214  Bit Score: 44.03  E-value: 1.29e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 247 GSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQ-TLELEPAPAPVPSLQP----SWPSPVVAENGLS 321
Cdd:PRK04654 130 GAEPGAGQAHTPVPAPAPVIAQAQPIAPAPHQTLVPAPHDTIVPAPhAAHLPSAPATPVSVAPvdagTSASPTPSEPTKI 209


                 ...
gi 108796058 322 EEK 324
Cdd:PRK04654 210 QEK 212
PRK12323 PRK12323
DNA polymerase III subunit gamma/tau;
198-347 1.83e-04

DNA polymerase III subunit gamma/tau;


Pssm-ID: 237057 [Multi-domain]  Cd Length: 700  Bit Score: 45.25  E-value: 1.83e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 198 PIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAvglesAPAPA 277
Cdd:PRK12323 392 PAAAAPAPAAPPAAPAAAPAAAAAARAVAAAPARRSPAPEALAAARQASARGPGGAPAPAPAPAAAPAAA-----ARPAA 466

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 278 LELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWpspvvaenglSEEKPHLLVFPPDLVAEQFTLMDAELF 347
Cdd:PRK12323 467 AGPRPVAAAAAAAPARAAPAAAPAPADDDPPPW----------EELPPEFASPAPAQPDAAPAGWVAESI 526
PRK11633 PRK11633
cell division protein DedD; Provisional
 247-308 2.60e-04

cell division protein DedD; Provisional


Pssm-ID: 236940 [Multi-domain]  Cd Length: 226  Bit Score: 43.45  E-value: 2.60e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 108796058 247 GSELEVAPAPAPELQQ----APEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQP 308
Cdd:PRK11633  72 AEAVRAGDAAAPSLDPatvaPPNTPVEPEPAPVEPPKPKPVEKPKPKPKPQQKVEAPPAPKPEPKP 137
PRK12323 PRK12323
DNA polymerase III subunit gamma/tau;
196-344 4.22e-04

DNA polymerase III subunit gamma/tau;


Pssm-ID: 237057 [Multi-domain]  Cd Length: 700  Bit Score: 44.10  E-value: 4.22e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 196 SEPIEAEPEALSPVPALKPTPELELAltparapSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPA 275
Cdd:PRK12323 379 AAPVAQPAPAAAAPAAAAPAPAAPPA-------APAAAPAAAAAARAVAAAPARRSPAPEALAAARQASARGPGGAPAPA 451

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 108796058 276 PALELEPAPEQDPAPSQtleLEPAPAPVPSLQPSwPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDA 344
Cdd:PRK12323 452 PAPAAAPAAAARPAAAG---PRPVAAAAAAAPAR-AAPAAAPAPADDDPPPWEELPPEFASPAPAQPDA 516
PHA03291 PHA03291
envelope glycoprotein I; Provisional
 253-325 1.61e-03

envelope glycoprotein I; Provisional


Pssm-ID: 223033 [Multi-domain]  Cd Length: 401  Bit Score: 41.86  E-value: 1.61e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 108796058 253 APAPAPELQQApepAVGLESAPAPALELEPAPEQDPAPSQTleLEPAPAPVPSLQPSWPSPVVAENGLSEEKP 325
Cdd:PHA03291 190 LPLSAPRLGPA---DVFVPATPRPTPRTTASPETTPTPSTT--TSPPSTTIPAPSTTIAAPQAGTTPEAEGTP 257
COG5373 COG5373
Uncharacterized membrane protein [Function unknown];
248-321 2.45e-03

Uncharacterized membrane protein [Function unknown];


Pssm-ID: 444140 [Multi-domain]  Cd Length: 854  Bit Score: 41.52  E-value: 2.45e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 108796058 248 SELE---VAPAPAPELQQAPEP-AVGLESAPAPALELEPAPEQDPAPSQTLElepAPAPVPSLQPSWPSPVVAENGLS 321
Cdd:COG5373   31 EELEaelAEAAEAASAPAEPEPeAAAAATAAAPEAAPAPVPEAPAAPPAAAE---APAPAAAAPPAEAEPAAAPAAAS 105
Rib_recp_KP_reg pfam05104
Ribosome receptor lysine/proline rich region; This highly conserved region is found towards ...
 256-308 2.57e-03

Ribosome receptor lysine/proline rich region; This highly conserved region is found towards the C-terminus of the transmembrane domain. The function is unclear.


Pssm-ID: 461548 [Multi-domain]  Cd Length: 140  Bit Score: 38.95  E-value: 2.57e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 108796058  256 PAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQP 308
Cdd:pfam05104  55 QADESEEEPREFKTPDEAPSAALEPEPVPTPVPAPVEPEPAPPSESPAPSPKE 107
RA cd17043
Ras-associating (RA) domain, structurally similar to a beta-grasp ubiquitin-like fold; RA ...
 746-827 2.58e-03

Ras-associating (RA) domain, structurally similar to a beta-grasp ubiquitin-like fold; RA domain-containing proteins function by interacting with Ras proteins directly or indirectly and are involved in various functions ranging from tumor suppression to being oncoproteins. Ras proteins are small GTPases that are involved in cellular signal transduction. The RA domain has the beta-grasp ubiquitin-like (Ubl) fold with low sequence similarity to ubiquitin (Ub); Ub is a protein modifier in eukaryotes that is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. RA-containing proteins include RalGDS, AF6, RIN, RASSF1, SNX27, CYR1, STE50, and phospholipase C epsilon.


Pssm-ID: 340563  Cd Length: 87  Bit Score: 37.68  E-value: 2.58e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 746 IIRVSLDVDNGN-MYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYD-- 822
Cdd:cd17043    1 VLKVYDDDLAPGsAYKSILVSSTTTAREVVQLLLEKYGLEEDPEDYSLYEVSEKQETERVLHDDECPLLIQLEWGPQGte 80


                 ....*..
gi 108796058 823 --FVLKK 827
Cdd:cd17043   81 frFVLKR 87
PHA03378 PHA03378
EBNA-3B; Provisional
 198-309 3.93e-03

EBNA-3B; Provisional


Pssm-ID: 223065 [Multi-domain]  Cd Length: 991  Bit Score: 40.82  E-value: 3.93e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 198 PIEAEPEALSPVPALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGlesAPAPa 277
Cdd:PHA03378 701 PTPMRPPAAPPGRAQRPAAATGRARPPAAAPGRARPPAAAPGRARPPAAAPGRARPPAAAPGRARPPAAAPG---APTP- 776

                         90       100       110
                 ....*....|....*....|....*....|..
gi 108796058 278 lelEPAPEQDPAPSQTLELEPAPAPVPSLQPS 309
Cdd:PHA03378 777 ---QPPPQAPPAPQQRPRGAPTPQPPPQAGPT 805
PRK11633 PRK11633
cell division protein DedD; Provisional
 249-325 4.24e-03

cell division protein DedD; Provisional


Pssm-ID: 236940 [Multi-domain]  Cd Length: 226  Bit Score: 39.60  E-value: 4.24e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 249 ELEVAPAPAPEL-QQAPEPAV-GLESAPAPALELEPA--------PEQDPAPSQTLELEPAPAPVPSLQPSwPSPVVAEN 318
Cdd:PRK11633  52 EPDMMPAATQALpTQPPEGAAeAVRAGDAAAPSLDPAtvappntpVEPEPAPVEPPKPKPVEKPKPKPKPQ-QKVEAPPA 130


                 ....*..
gi 108796058 319 GLSEEKP 325
Cdd:PRK11633 131 PKPEPKP 137
PRK14951 PRK14951
DNA polymerase III subunits gamma and tau; Provisional
 253-337 5.31e-03

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 237865 [Multi-domain]  Cd Length: 618  Bit Score: 40.47  E-value: 5.31e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 253 APAPAPELQQAPEPAVGLESAPAPalELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPP 332
Cdd:PRK14951 370 AEAAAPAEKKTPARPEAAAPAAAP--VAQAAAAPAPAAAPAAAASAPAAPPAAAPPAPVAAPAAAAPAAAPAAAPAAVAL 447


                 ....*
gi 108796058 333 DLVAE 337
Cdd:PRK14951 448 APAPP 452
PRK14954 PRK14954
DNA polymerase III subunits gamma and tau; Provisional
 251-314 6.44e-03

DNA polymerase III subunits gamma and tau; Provisional


Pssm-ID: 184918 [Multi-domain]  Cd Length: 620  Bit Score: 39.93  E-value: 6.44e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 108796058 251 EVAPAPA---PELQQAPEPavglesaPAPALELEPAPEQDPAPSQTLELEPAPA--PVPSLQPSWPSPV 314
Cdd:PRK14954 379 GVAPSPAgspDVKKKAPEP-------DLPQPDRHPGPAKPEAPGARPAELPSPAsaPTPEQQPPVARSA 440
Trypan_PARP pfam05887
Procyclic acidic repetitive protein (PARP); This family consists of several Trypanosoma brucei ...
 192-306 8.28e-03

Procyclic acidic repetitive protein (PARP); This family consists of several Trypanosoma brucei procyclic acidic repetitive protein (PARP) like sequences. The procyclic acidic repetitive protein (parp) genes of Trypanosoma brucei encode a small family of abundant surface proteins whose expression is restricted to the procyclic form of the parasite. They are found at two unlinked loci, parpA and parpB; transcription of both loci is developmentally regulated.


Pssm-ID: 368653  Cd Length: 134  Bit Score: 37.46  E-value: 8.28e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058  192 QLEHSEPIEAEPEalsPVPALKPTPElelaltparapspvpapapepepaptpapgselevaPAPAPELQQAPEPAVGLE 271
Cdd:pfam05887  50 SDDDTNGTDPEPE---PEPEPEPEPE------------------------------------PEPEPEPEPEPEPEPEPE 90

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 108796058  272 SAPAPALELEPAPEQDPAPsqtlelEPAPAPVPSL 306
Cdd:pfam05887  91 PEPEPEPEPEPEPEPEPEP------EPGAATLKSV 119
DamX COG3266
Cell division protein DamX, binds to the septal ring, contains C-terminal SPOR domain [Cell ...
 166-341 8.31e-03

Cell division protein DamX, binds to the septal ring, contains C-terminal SPOR domain [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 442497 [Multi-domain]  Cd Length: 455  Bit Score: 39.45  E-value: 8.31e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 166 LKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPE-----ALSPVPALKPTPELELALTPARAPSPVPAPAPEPEP 240
Cdd:COG3266  187 RKAEEALALRAGSAAADALALLLLLLASALGEAVAAAAElaalaLLAAGAAEVLTARLVLLLLIIGSALKAPSQASSASA 266

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 108796058 241 APTPAPGSELEVAPAPAPELQ------------QAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQP 308
Cdd:COG3266  267 PATTSLGEQQEVSLPPAVAAQpaaaaaaqpsavALPAAPAAAAAAAAPAEAAAPQPTAAKPVVTETAAPAAPAPEAAAAA 346

                        170       180       190
                 ....*....|....*....|....*....|...
gi 108796058 309 SWPSPVVAENGLSEEKPHLLVFPPDlvaeQFTL 341
Cdd:COG3266  347 AAPAAPAVAKKLAADEQWLASQPAS----HYTL 375
PRK07003 PRK07003
DNA polymerase III subunit gamma/tau;
247-318 8.42e-03

DNA polymerase III subunit gamma/tau;


Pssm-ID: 235906 [Multi-domain]  Cd Length: 830  Bit Score: 39.83  E-value: 8.42e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 108796058 247 GSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAEN 318
Cdd:PRK07003 419 AATRAEAPPAAPAPPATADRGDDAADGDAPVPAKANARASADSRCDERDAQPPADSGSASAPASDAPPDAAF 490
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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