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Conserved domains on  [gi|287327510|ref|NP_001098598|]
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methionine-R-sulfoxide reductase B1b [Danio rerio]

Protein Classification

peptide-methionine (R)-S-oxide reductase( domain architecture ID 81886)

peptide-methionine (R)-S-oxide reductase catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins

CATH:  2.170.150.20
EC:  1.8.4.12
Gene Ontology:  GO:0046872|GO:0033743|GO:0030091|GO:0034599
PubMed:  11169920
SCOP:  4002166

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SelR super family cl15841
SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate ...
 12-106 1.49e-29

SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate biological processes and cope with oxidative stress. MsrA, a protein involved in the reduction of methionine sulfoxides in proteins, has been known for four decades and has been extensively characterized with respect to structure and function. However, recent studies revealed that MsrA is only specific for methionine-S-sulfoxides. Because oxidized methionines occur in a mixture of R and S isomers in vivo, it was unclear how stereo-specific MsrA could be responsible for the reduction of all protein methionine sulfoxides. It appears that a second methionine sulfoxide reductase, SelR, evolved that is specific for methionine-R-sulfoxides, the activity that is different but complementary to that of MsrA. Thus, these proteins, working together, could reduce both stereoisomers of methionine sulfoxide. This domain is found both in SelR proteins and fused with the peptide methionine sulfoxide reductase enzymatic domain pfam01625. The domain has two conserved cysteine and histidines. The domain binds both selenium and zinc. The final cysteine is found to be replaced by the rare amino acid selenocysteine in some members of the family. This family has methionine-R-sulfoxide reductase activity.


The actual alignment was detected with superfamily member COG0229:

Pssm-ID: 472838  Cd Length: 133  Bit Score: 102.85  E-value: 1.49e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510  12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTK-------MMETltayKVLCGKCGNGLGHEFlNDG 84
Cdd:COG0229   37 YWDNKEEGIYVCAGCGAPLFSSDTKFDSGTGWPSFTKPIDPGAVEEkedrshgMVRT----EVRCARCGAHLGHVF-DDG 111

                         90       100
                 ....*....|....*....|...
gi 287327510  85 PEE-GLsRFUIFSHSLKFVPKDK 106
Cdd:COG0229  112 PPPtGL-RYCINSAALRFIPKEE 133
 
Name Accession Description Interval E-value
MsrB COG0229
Peptide methionine sulfoxide reductase MsrB [Posttranslational modification, protein turnover, ...
 12-106 1.49e-29

Peptide methionine sulfoxide reductase MsrB [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 439999  Cd Length: 133  Bit Score: 102.85  E-value: 1.49e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510  12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTK-------MMETltayKVLCGKCGNGLGHEFlNDG 84
Cdd:COG0229   37 YWDNKEEGIYVCAGCGAPLFSSDTKFDSGTGWPSFTKPIDPGAVEEkedrshgMVRT----EVRCARCGAHLGHVF-DDG 111

                         90       100
                 ....*....|....*....|...
gi 287327510  85 PEE-GLsRFUIFSHSLKFVPKDK 106
Cdd:COG0229  112 PPPtGL-RYCINSAALRFIPKEE 133
TIGR00357 TIGR00357
methionine-R-sulfoxide reductase; This model describes a domain found in PilB, a protein ...
 12-109 5.69e-27

methionine-R-sulfoxide reductase; This model describes a domain found in PilB, a protein important for pilin expression, N-terminal to a domain coextensive to with the known peptide methionine sulfoxide reductase (MsrA), a protein repair enzyme, of E. coli. Among the early completed genomes, this module is found if and only if MsrA is also found, whether N-terminal to MsrA (as for Helicobacter pylori), C-terminal (as for Treponema pallidum), or in a separate polypeptide. Although the function of this region is not clear, an auxiliary function to MsrA is suggested. [Protein fate, Protein modification and repair, Cellular processes, Adaptations to atypical conditions]


Pssm-ID: 129454  Cd Length: 134  Bit Score: 96.38  E-value: 5.69e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMET---LTAYKVLCGKCGNGLGHEFlNDGPEEG 88
Cdd:TIGR00357  32 YWDNKEEGIYVDITCGEPLFSSEDKFDSGCGWPSFYKPISEEVVAYERDEshgMIRTEVRCRNCDAHLGHVF-DDGPEPT 110

                          90       100
                  ....*....|....*....|.
gi 287327510   89 LSRFUIFSHSLKFVPKDKVDK 109
Cdd:TIGR00357 111 GLRYCINSAALKFIPLEEMEE 131
SelR pfam01641
SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate ...
 12-103 9.58e-27

SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate biological processes and cope with oxidative stress. MsrA, a protein involved in the reduction of methionine sulfoxides in proteins, has been known for four decades and has been extensively characterized with respect to structure and function. However, recent studies revealed that MsrA is only specific for methionine-S-sulfoxides. Because oxidized methionines occur in a mixture of R and S isomers in vivo, it was unclear how stereo-specific MsrA could be responsible for the reduction of all protein methionine sulfoxides. It appears that a second methionine sulfoxide reductase, SelR, evolved that is specific for methionine-R-sulfoxides, the activity that is different but complementary to that of MsrA. Thus, these proteins, working together, could reduce both stereoisomers of methionine sulfoxide. This domain is found both in SelR proteins and fused with the peptide methionine sulfoxide reductase enzymatic domain pfam01625. The domain has two conserved cysteine and histidines. The domain binds both selenium and zinc. The final cysteine is found to be replaced by the rare amino acid selenocysteine in some members of the family. This family has methionine-R-sulfoxide reductase activity.


Pssm-ID: 460278  Cd Length: 120  Bit Score: 95.12  E-value: 9.58e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTK-------MMETltayKVLCGKCGNGLGHEFlNDG 84
Cdd:pfam01641  27 YWDNKEPGIYVCAGCGTPLFSSDTKFDSGCGWPSFYDPIPGDAVKEkedtshgMVRT----EVRCARCGGHLGHVF-DDG 101

                          90       100
                  ....*....|....*....|
gi 287327510   85 PEE-GLsRFUIFSHSLKFVP 103
Cdd:pfam01641 102 PPPtGL-RYCINSASLKFIP 120
PRK05550 PRK05550
bifunctional methionine sulfoxide reductase B/A protein; Provisional
 6-106 1.16e-20

bifunctional methionine sulfoxide reductase B/A protein; Provisional


Pssm-ID: 235499 [Multi-domain]  Cd Length: 283  Bit Score: 83.41  E-value: 1.16e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   6 FFGKefYKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETI-----RE-DSVTKMMEtltaykVLCGKCGNGLGHE 79
Cdd:PRK05550  24 FSGE--YYDHDEKGVYLCRRCGAPLFRSEDKFNSGCGWPSFDDEIpgavkRLpDADGRRTE------IVCANCGAHLGHV 95

                         90       100
                 ....*....|....*....|....*..
gi 287327510  80 FLNDGPEEGLSRFUIFSHSLKFVPKDK 106
Cdd:PRK05550  96 FEGEGLTPKNTRHCVNSASLDFVPAEE 122
 
Name Accession Description Interval E-value
MsrB COG0229
Peptide methionine sulfoxide reductase MsrB [Posttranslational modification, protein turnover, ...
 12-106 1.49e-29

Peptide methionine sulfoxide reductase MsrB [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 439999  Cd Length: 133  Bit Score: 102.85  E-value: 1.49e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510  12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTK-------MMETltayKVLCGKCGNGLGHEFlNDG 84
Cdd:COG0229   37 YWDNKEEGIYVCAGCGAPLFSSDTKFDSGTGWPSFTKPIDPGAVEEkedrshgMVRT----EVRCARCGAHLGHVF-DDG 111

                         90       100
                 ....*....|....*....|...
gi 287327510  85 PEE-GLsRFUIFSHSLKFVPKDK 106
Cdd:COG0229  112 PPPtGL-RYCINSAALRFIPKEE 133
TIGR00357 TIGR00357
methionine-R-sulfoxide reductase; This model describes a domain found in PilB, a protein ...
 12-109 5.69e-27

methionine-R-sulfoxide reductase; This model describes a domain found in PilB, a protein important for pilin expression, N-terminal to a domain coextensive to with the known peptide methionine sulfoxide reductase (MsrA), a protein repair enzyme, of E. coli. Among the early completed genomes, this module is found if and only if MsrA is also found, whether N-terminal to MsrA (as for Helicobacter pylori), C-terminal (as for Treponema pallidum), or in a separate polypeptide. Although the function of this region is not clear, an auxiliary function to MsrA is suggested. [Protein fate, Protein modification and repair, Cellular processes, Adaptations to atypical conditions]


Pssm-ID: 129454  Cd Length: 134  Bit Score: 96.38  E-value: 5.69e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMET---LTAYKVLCGKCGNGLGHEFlNDGPEEG 88
Cdd:TIGR00357  32 YWDNKEEGIYVDITCGEPLFSSEDKFDSGCGWPSFYKPISEEVVAYERDEshgMIRTEVRCRNCDAHLGHVF-DDGPEPT 110

                          90       100
                  ....*....|....*....|.
gi 287327510   89 LSRFUIFSHSLKFVPKDKVDK 109
Cdd:TIGR00357 111 GLRYCINSAALKFIPLEEMEE 131
SelR pfam01641
SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate ...
 12-103 9.58e-27

SelR domain; Methionine sulfoxide reduction is an important process, by which cells regulate biological processes and cope with oxidative stress. MsrA, a protein involved in the reduction of methionine sulfoxides in proteins, has been known for four decades and has been extensively characterized with respect to structure and function. However, recent studies revealed that MsrA is only specific for methionine-S-sulfoxides. Because oxidized methionines occur in a mixture of R and S isomers in vivo, it was unclear how stereo-specific MsrA could be responsible for the reduction of all protein methionine sulfoxides. It appears that a second methionine sulfoxide reductase, SelR, evolved that is specific for methionine-R-sulfoxides, the activity that is different but complementary to that of MsrA. Thus, these proteins, working together, could reduce both stereoisomers of methionine sulfoxide. This domain is found both in SelR proteins and fused with the peptide methionine sulfoxide reductase enzymatic domain pfam01625. The domain has two conserved cysteine and histidines. The domain binds both selenium and zinc. The final cysteine is found to be replaced by the rare amino acid selenocysteine in some members of the family. This family has methionine-R-sulfoxide reductase activity.


Pssm-ID: 460278  Cd Length: 120  Bit Score: 95.12  E-value: 9.58e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   12 YKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTK-------MMETltayKVLCGKCGNGLGHEFlNDG 84
Cdd:pfam01641  27 YWDNKEPGIYVCAGCGTPLFSSDTKFDSGCGWPSFYDPIPGDAVKEkedtshgMVRT----EVRCARCGGHLGHVF-DDG 101

                          90       100
                  ....*....|....*....|
gi 287327510   85 PEE-GLsRFUIFSHSLKFVP 103
Cdd:pfam01641 102 PPPtGL-RYCINSASLKFIP 120
PRK05550 PRK05550
bifunctional methionine sulfoxide reductase B/A protein; Provisional
 6-106 1.16e-20

bifunctional methionine sulfoxide reductase B/A protein; Provisional


Pssm-ID: 235499 [Multi-domain]  Cd Length: 283  Bit Score: 83.41  E-value: 1.16e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   6 FFGKefYKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETI-----RE-DSVTKMMEtltaykVLCGKCGNGLGHE 79
Cdd:PRK05550  24 FSGE--YYDHDEKGVYLCRRCGAPLFRSEDKFNSGCGWPSFDDEIpgavkRLpDADGRRTE------IVCANCGAHLGHV 95

                         90       100
                 ....*....|....*....|....*..
gi 287327510  80 FLNDGPEEGLSRFUIFSHSLKFVPKDK 106
Cdd:PRK05550  96 FEGEGLTPKNTRHCVNSASLDFVPAEE 122
PRK05508 PRK05508
methionine-R-sulfoxide reductase;
6-106 2.40e-19

methionine-R-sulfoxide reductase;


Pssm-ID: 180121  Cd Length: 119  Bit Score: 76.29  E-value: 2.40e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   6 FFGKefYKDHFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIrEDSVTKMMETlTAYK--VLCGKCGNGLGHEFLND 83
Cdd:PRK05508  21 FSGE--YNDFFEKGTYVCKQCGAPLYRSEDKFKSGCGWPSFDDEI-KGAVKRIPDA-DGRRteIVCANCGGHLGHVFEGE 96

                         90       100
                 ....*....|....*....|...
gi 287327510  84 GPEEGLSRFUIFSHSLKFVPKDK 106
Cdd:PRK05508  97 GFTPKNTRHCVNSISLKFVPDKG 119
PRK14018 PRK14018
bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide ...
 7-109 8.76e-17

bifunctional peptide-methionine (S)-S-oxide reductase MsrA/peptide-methionine (R)-S-oxide reductase MsrB;


Pssm-ID: 184456 [Multi-domain]  Cd Length: 521  Bit Score: 74.14  E-value: 8.76e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 287327510   7 FGKEFYKDhFKPGIYACSQCGHPLFSSRSKYEHSSPWPAFTETIREDSVTKMMET---LTAYKVLCGKCGNGLGHEFlND 83
Cdd:PRK14018 406 FSHEYDHL-FKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPIDAKVVTEHDDFsynMRRTEVRSRAADSHLGHVF-PD 483

                         90       100
                 ....*....|....*....|....*...
gi 287327510  84 GPEE--GLsRFUIFSHSLKFVPKDKVDK 109
Cdd:PRK14018 484 GPRDkgGL-RYCINGASLKFIPLEQMDA 510
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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