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Conserved domains on  [gi|187169264|ref|NP_001119601|]
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DNA replication complex GINS protein PSF3 isoform a [Homo sapiens]

Protein Classification

DNA replication complex GINS protein PSF3( domain architecture ID 16902678)

DNA replication complex GINS protein PSF3 is a component of the heterotetrameric GINS complex; the GINS complex is essential for both the initiation and elongation stages of eukaryotic DNA replication

CATH:  1.20.58.2050
Gene Ontology:  GO:0006260
PubMed:  22918584|20070258

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 122-231 2.27e-46

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


:

Pssm-ID: 212551  Cd Length: 109  Bit Score: 150.11  E-value: 2.27e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264 122 ILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDsPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTS 201
Cdd:cd11713    1 FVSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLF-PEDEELAEVLLETFKERFREIMDHAQNASNEDNS 79

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264 202 ALVARLDEMERGLFQTGQKGLNDFQCWEKG 231
Cdd:cd11713   80 EFLRKLDELERKLFRAGQESLKDFKKWLKG 109
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 19-128 1.46e-14

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


:

Pssm-ID: 412029  Cd Length: 64  Bit Score: 66.41  E-value: 1.46e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  19 FLSLDDILMSHEKLPVRTETAMPRLGafFLErsAGAETDNavpqgfallprlecsgviwltaaltsqapeilppqppmwl 98
Cdd:cd21693    1 YYDIDDILAEEEKVPCRFEVDLPGLG--FLD--PSSDDDD---------------------------------------- 36

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264  99 VLQGSKLELPLWLAKGLFDnkRRILSVELP 128
Cdd:cd21693   37 LPPGTKVELPLWLAKALAR--RRIVSVELP 64
 
Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 122-231 2.27e-46

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212551  Cd Length: 109  Bit Score: 150.11  E-value: 2.27e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264 122 ILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDsPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTS 201
Cdd:cd11713    1 FVSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLF-PEDEELAEVLLETFKERFREIMDHAQNASNEDNS 79

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264 202 ALVARLDEMERGLFQTGQKGLNDFQCWEKG 231
Cdd:cd11713   80 EFLRKLDELERKLFRAGQESLKDFKKWLKG 109
COG5138 COG5138
Uncharacterized conserved protein [Function unknown];
94-228 1.24e-14

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227467 [Multi-domain]  Cd Length: 168  Bit Score: 69.55  E-value: 1.24e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  94 PPMWLVLQGSKLELPLWLAKGLFDNKrrILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSpeNADI 173
Cdd:COG5138   32 PRGRILPENRKVELPYWLASFLAINS--FVCELGPGFFSESVLNAIKAKPSTVDLRSICPHFFYLAEKYAMLLS--EGSL 107

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 187169264 174 SQSLLQTFIGRFRRIMDSSQNA--YNEDTSALVARLDEMERGLFQTGQKGLNDFQCW 228
Cdd:COG5138  108 VAGLPEFLYERAGDYSSASLNLkgTFSENSQFVWRLDMVEKSIVVGSHRRFQRFCAW 164
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 19-128 1.46e-14

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


Pssm-ID: 412029  Cd Length: 64  Bit Score: 66.41  E-value: 1.46e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  19 FLSLDDILMSHEKLPVRTETAMPRLGafFLErsAGAETDNavpqgfallprlecsgviwltaaltsqapeilppqppmwl 98
Cdd:cd21693    1 YYDIDDILAEEEKVPCRFEVDLPGLG--FLD--PSSDDDD---------------------------------------- 36

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264  99 VLQGSKLELPLWLAKGLFDnkRRILSVELP 128
Cdd:cd21693   37 LPPGTKVELPLWLAKALAR--RRIVSVELP 64
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 105-212 2.38e-12

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 61.74  E-value: 2.38e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  105 LELPLWLAKGLFdnKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSpeNADISQSLLQTFIGR 184
Cdd:pfam05916   1 PELPLWLAELLK--RRGLVEIEPPEWLSIEELEAILADETKNDLSLLPPYFYELAVLLLELER--DDELKRLLRDYLRIR 76

                          90       100
                  ....*....|....*....|....*....
gi 187169264  185 FRRIMDSSQNAYN-EDTSALVARLDEMER 212
Cdd:pfam05916  77 LAKIRKLAWHLNGsLSPSDLLDNLSEEEL 105
 
Name Accession Description Interval E-value
GINS_A_psf3 cd11713
Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of ...
 122-231 2.27e-46

Alpha-helical domain of GINS complex protein Psf3 (partner of Sld5 3); Psf3 is a component of GINS, a tetrameric protein complex. Psf3 expression is up regulated in malignant colon cancer and it might be involved in cancer cell proliferation. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212551  Cd Length: 109  Bit Score: 150.11  E-value: 2.27e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264 122 ILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDsPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTS 201
Cdd:cd11713    1 FVSVELPKIYSEKVRNALKADPTSVDLHKLSPYFYELGLKLLSLF-PEDEELAEVLLETFKERFREIMDHAQNASNEDNS 79

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264 202 ALVARLDEMERGLFQTGQKGLNDFQCWEKG 231
Cdd:cd11713   80 EFLRKLDELERKLFRAGQESLKDFKKWLKG 109
COG5138 COG5138
Uncharacterized conserved protein [Function unknown];
94-228 1.24e-14

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227467 [Multi-domain]  Cd Length: 168  Bit Score: 69.55  E-value: 1.24e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  94 PPMWLVLQGSKLELPLWLAKGLFDNKrrILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSpeNADI 173
Cdd:COG5138   32 PRGRILPENRKVELPYWLASFLAINS--FVCELGPGFFSESVLNAIKAKPSTVDLRSICPHFFYLAEKYAMLLS--EGSL 107

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 187169264 174 SQSLLQTFIGRFRRIMDSSQNA--YNEDTSALVARLDEMERGLFQTGQKGLNDFQCW 228
Cdd:COG5138  108 VAGLPEFLYERAGDYSSASLNLkgTFSENSQFVWRLDMVEKSIVVGSHRRFQRFCAW 164
GINS_B_Psf3 cd21693
beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the ...
 19-128 1.46e-14

beta-strand (B) domain of GINS complex protein Psf3; Psf3 (partner of Sld5 3) is one of the proteins known to comprise the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) complex, which is a macromolecular protein complex associated with DNA replication. Psf3 is dysregulated in cancer cells, and its overexpression may be related to tumor progression in some cancers including colon, breast, and lung cancers; its expression can be used as a prognostic indicator in some cancers. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2, and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf3.


Pssm-ID: 412029  Cd Length: 64  Bit Score: 66.41  E-value: 1.46e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  19 FLSLDDILMSHEKLPVRTETAMPRLGafFLErsAGAETDNavpqgfallprlecsgviwltaaltsqapeilppqppmwl 98
Cdd:cd21693    1 YYDIDDILAEEEKVPCRFEVDLPGLG--FLD--PSSDDDD---------------------------------------- 36

                         90       100       110
                 ....*....|....*....|....*....|
gi 187169264  99 VLQGSKLELPLWLAKGLFDnkRRILSVELP 128
Cdd:cd21693   37 LPPGTKVELPLWLAKALAR--RRIVSVELP 64
GINS_A cd11581
Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is ...
 133-224 1.69e-12

Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. In eukaryotes, GINS is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3. The GINS complex has been found in eukaryotes and archaea, but not in bacteria. The four subunits of the complex are homologous and consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212547  Cd Length: 103  Bit Score: 62.13  E-value: 1.69e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264 133 EGWRTVFSAD----PNVVDLHkmgPHFYGFGSQLLHFDS-----------------PENADISQSLLQTFIGRFRRIMDS 191
Cdd:cd11581    1 VEKLAEAIHNerksENLLPPP---PDFMEEVKELLHRLSkrqrsetqeilehrlkiLELSYIRDLLKTYLNSRLDKIRDL 77

                         90       100       110
                 ....*....|....*....|....*....|...
gi 187169264 192 SQNaynedtsALVARLDEMERGLFQTGQKGLND 224
Cdd:cd11581   78 SPE-------FLPSNLTEEEIGYFQTYWKEVAE 103
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 105-212 2.38e-12

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 61.74  E-value: 2.38e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 187169264  105 LELPLWLAKGLFdnKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSpeNADISQSLLQTFIGR 184
Cdd:pfam05916   1 PELPLWLAELLK--RRGLVEIEPPEWLSIEELEAILADETKNDLSLLPPYFYELAVLLLELER--DDELKRLLRDYLRIR 76

                          90       100
                  ....*....|....*....|....*....
gi 187169264  185 FRRIMDSSQNAYN-EDTSALVARLDEMER 212
Cdd:pfam05916  77 LAKIRKLAWHLNGsLSPSDLLDNLSEEEL 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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