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Conserved domains on  [gi|209969830|ref|NP_001129665|]
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FAST kinase domain-containing protein 2, mitochondrial [Homo sapiens]

Protein Classification

RAP domain-containing protein; endonuclease( domain architecture ID 10535838)

RAP domain-containing protein binds RNA, similar to Arabidopsis thaliana RAP domain-containing protein (AtRAP) that binds to the 16S rRNA precursor, is involved in its 5'-end processing, and is required for chloroplast 16S rRNA maturation, an important process which supports chloroplast gene expression and biogenesis; uncharacterized endonuclease similar to NucS, which cleaves both 3' and 5' ssDNA extremities of branched DNA structures

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RESC6-like super family cl46254
RNA-editing substrate-binding complex subunit 6 (RESC6) and related proteins; RESC6 (GRBC6, ...
 181-407 1.11e-35

RNA-editing substrate-binding complex subunit 6 (RESC6) and related proteins; RESC6 (GRBC6, MRB3010) is a component of the RNA-editing substrate-binding complex (RESC), that is composed of about 20 components and is involved in kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. RESC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and RNA-editing catalytic complex (RECC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing. RESC6 is predicted (by AlphaFold) to adopt an all-helical structure with a disordered region at the N-terminus. The helical structure that is predicted with high confidence resembles Armadillo (ARM)/beta-catenin-like repeats. ARM repeats have been implicated in mediating protein-protein interactions.


The actual alignment was detected with superfamily member cd23739:

Pssm-ID: 480594 [Multi-domain]  Cd Length: 249  Bit Score: 135.08  E-value: 1.11e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 181 SSNYFTAMWTIAKRLSDDQKRfEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQE 260
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRA-EKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRW 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 261 RINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPiALKRKLEMKALRELDRF 340
Cdd:cd23739   80 RLRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSP-LLMNRLEDKALELLEQF 158

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 209969830 341 SVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYV 407
Cdd:cd23739  159 TLEELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADL 225
FAST_1 pfam06743
FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic ...
 456-529 1.26e-21

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.


:

Pssm-ID: 461999 [Multi-domain]  Cd Length: 72  Bit Score: 89.08  E-value: 1.26e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 209969830  456 ILSILHTYSSLNhvYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELL 529
Cdd:pfam06743   1 LLSLLLPFARLN--YLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72
RAP smart00952
This domain is found in various eukaryotic species, particularly in apicomplexans; In ...
 636-692 7.23e-15

This domain is found in various eukaryotic species, particularly in apicomplexans; In Plasmodium falciparum, the domain is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain.


:

Pssm-ID: 214932  Cd Length: 58  Bit Score: 69.18  E-value: 7.23e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 209969830   636 VLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDA-VTFLKTK 692
Cdd:smart00952   1 IEVDGPSHFCRNSRHLLGRLALKKRHLEALGYKVISIPYYEWNKLKSEDEkVEYLKEK 58
FAST_2 super family cl07113
FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic ...
 578-619 5.13e-08

FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This subdomain is often found associated with the FAST kinase-like protein, subdomain 2.


The actual alignment was detected with superfamily member pfam08368:

Pssm-ID: 462448  Cd Length: 89  Bit Score: 51.03  E-value: 5.13e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 209969830  578 VAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNrNQVLP 619
Cdd:pfam08368  49 VLEALKELLGGENYFRSNVLTPYGYTIDFECKLDKD-GKPLP 89
 
Name Accession Description Interval E-value
TBRG4-like_N cd23739
transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted ...
 181-407 1.11e-35

transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted alpha-helical domain N-terminal to FAST kinase-like protein, subdomain 1 (FAST_1); TBRG4 also called cell cycle progression restoration protein 2 or Fas-activated serine-threonine (FAST) kinase domain-containing protein 4, is a regulator of TGFbeta and is implicated in numerous types of cancer. It serves a role in the tumorigenesis of lung cancer via deregulation of DNA damage-inducible transcript 3 (DDIT3), caveolin 1 (CAV1), and ribonucleotide reductase regulatory subunit M2 (RRM2). This domain lies N-terminal to the FAST_1 domain, and is predicted (by AlphaFold) to be alpha-helical, with similarity to structures of members of the 6-hairpin (6 helical hairpin) clan.


Pssm-ID: 467932 [Multi-domain]  Cd Length: 249  Bit Score: 135.08  E-value: 1.11e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 181 SSNYFTAMWTIAKRLSDDQKRfEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQE 260
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRA-EKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRW 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 261 RINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPiALKRKLEMKALRELDRF 340
Cdd:cd23739   80 RLRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSP-LLMNRLEDKALELLEQF 158

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 209969830 341 SVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYV 407
Cdd:cd23739  159 TLEELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADL 225
FAST_1 pfam06743
FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic ...
 456-529 1.26e-21

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.


Pssm-ID: 461999 [Multi-domain]  Cd Length: 72  Bit Score: 89.08  E-value: 1.26e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 209969830  456 ILSILHTYSSLNhvYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELL 529
Cdd:pfam06743   1 LLSLLLPFARLN--YLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72
RAP smart00952
This domain is found in various eukaryotic species, particularly in apicomplexans; In ...
 636-692 7.23e-15

This domain is found in various eukaryotic species, particularly in apicomplexans; In Plasmodium falciparum, the domain is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain.


Pssm-ID: 214932  Cd Length: 58  Bit Score: 69.18  E-value: 7.23e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 209969830   636 VLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDA-VTFLKTK 692
Cdd:smart00952   1 IEVDGPSHFCRNSRHLLGRLALKKRHLEALGYKVISIPYYEWNKLKSEDEkVEYLKEK 58
RAP pfam08373
RAP domain; This domain is found in various eukaryotic species, where it is found in proteins ...
 636-692 2.22e-12

RAP domain; This domain is found in various eukaryotic species, where it is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain. The domain is involved in plant defence in response to bacterial infection.


Pssm-ID: 429953  Cd Length: 58  Bit Score: 62.21  E-value: 2.22e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 209969830  636 VLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLE-MEDAVTFLKTK 692
Cdd:pfam08373   1 IEVDGPSHFYRNSKQLTGSTKLKHRLLSALGYKVISIPYYEWNQLKtKEEKVEYLKKK 58
FAST_2 pfam08368
FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic ...
 578-619 5.13e-08

FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This subdomain is often found associated with the FAST kinase-like protein, subdomain 2.


Pssm-ID: 462448  Cd Length: 89  Bit Score: 51.03  E-value: 5.13e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 209969830  578 VAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNrNQVLP 619
Cdd:pfam08368  49 VLEALKELLGGENYFRSNVLTPYGYTIDFECKLDKD-GKPLP 89
 
Name Accession Description Interval E-value
TBRG4-like_N cd23739
transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted ...
 181-407 1.11e-35

transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted alpha-helical domain N-terminal to FAST kinase-like protein, subdomain 1 (FAST_1); TBRG4 also called cell cycle progression restoration protein 2 or Fas-activated serine-threonine (FAST) kinase domain-containing protein 4, is a regulator of TGFbeta and is implicated in numerous types of cancer. It serves a role in the tumorigenesis of lung cancer via deregulation of DNA damage-inducible transcript 3 (DDIT3), caveolin 1 (CAV1), and ribonucleotide reductase regulatory subunit M2 (RRM2). This domain lies N-terminal to the FAST_1 domain, and is predicted (by AlphaFold) to be alpha-helical, with similarity to structures of members of the 6-hairpin (6 helical hairpin) clan.


Pssm-ID: 467932 [Multi-domain]  Cd Length: 249  Bit Score: 135.08  E-value: 1.11e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 181 SSNYFTAMWTIAKRLSDDQKRfEKRLMFSHPAFNQLCEHMMREAKIMQYKYLLFSLHAIVKLGIPQNTILVQTLLRVTQE 260
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRA-EKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRW 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 209969830 261 RINECDEICLSVLSTVLEAMEPCKNVHVLRTGFRILVDQQVWKIEDVFTLQVVMKCIGKDAPiALKRKLEMKALRELDRF 340
Cdd:cd23739   80 RLRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSP-LLMNRLEDKALELLEQF 158

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 209969830 341 SVLNSQHMFEVLAAMNHRSLILLDECSKVVLDNIHGCPLRIMINILQSCKDLQYHNLDLFKGLADYV 407
Cdd:cd23739  159 TLEELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADL 225
FAST_1 pfam06743
FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic ...
 456-529 1.26e-21

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.


Pssm-ID: 461999 [Multi-domain]  Cd Length: 72  Bit Score: 89.08  E-value: 1.26e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 209969830  456 ILSILHTYSSLNhvYKCQNKEQFVEVMASALTGYLHTISSENLLDAVYSFCLMNYFPLAPFNQLLQKDIISELL 529
Cdd:pfam06743   1 LLSLLLPFARLN--YLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72
RAP smart00952
This domain is found in various eukaryotic species, particularly in apicomplexans; In ...
 636-692 7.23e-15

This domain is found in various eukaryotic species, particularly in apicomplexans; In Plasmodium falciparum, the domain is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain.


Pssm-ID: 214932  Cd Length: 58  Bit Score: 69.18  E-value: 7.23e-15
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 209969830   636 VLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLEMEDA-VTFLKTK 692
Cdd:smart00952   1 IEVDGPSHFCRNSRHLLGRLALKKRHLEALGYKVISIPYYEWNKLKSEDEkVEYLKEK 58
RAP pfam08373
RAP domain; This domain is found in various eukaryotic species, where it is found in proteins ...
 636-692 2.22e-12

RAP domain; This domain is found in various eukaryotic species, where it is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain. The domain is involved in plant defence in response to bacterial infection.


Pssm-ID: 429953  Cd Length: 58  Bit Score: 62.21  E-value: 2.22e-12
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 209969830  636 VLCVSRSAYCLGSSHPRGFLAMKMRHLNAMGFHVILVNNWEMDKLE-MEDAVTFLKTK 692
Cdd:pfam08373   1 IEVDGPSHFYRNSKQLTGSTKLKHRLLSALGYKVISIPYYEWNQLKtKEEKVEYLKKK 58
FAST_2 pfam08368
FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic ...
 578-619 5.13e-08

FAST kinase-like protein, subdomain 2; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This subdomain is often found associated with the FAST kinase-like protein, subdomain 2.


Pssm-ID: 462448  Cd Length: 89  Bit Score: 51.03  E-value: 5.13e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 209969830  578 VAEVLSSLLGGEGHFSKDVHLPHNYHIDFEIRMDTNrNQVLP 619
Cdd:pfam08368  49 VLEALKELLGGENYFRSNVLTPYGYTIDFECKLDKD-GKPLP 89
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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