transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted alpha-helical domain N-terminal to FAST kinase-like protein, subdomain 1 (FAST_1); TBRG4 also called cell cycle progression restoration protein 2 or Fas-activated serine-threonine (FAST) kinase domain-containing protein 4, is a regulator of TGFbeta and is implicated in numerous types of cancer. It serves a role in the tumorigenesis of lung cancer via deregulation of DNA damage-inducible transcript 3 (DDIT3), caveolin 1 (CAV1), and ribonucleotide reductase regulatory subunit M2 (RRM2). This domain lies N-terminal to the FAST_1 domain, and is predicted (by AlphaFold) to be alpha-helical, with similarity to structures of members of the 6-hairpin (6 helical hairpin) clan.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 107 DSNQAAMVLIRLSHLLSEKPEDKGLLIQDAHFHQLLCLLNSQIASVWHGTLSKLLGSLYALGIPKASKELQSVEQEVRWR 186
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRAEKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRWR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 187 MRKLKYKHLAFLAESCATLSQEQHSQELLAELLTHLERRWTEIEDSHTLVTVMMKVGHLSEPLMNRLEDKCLELVEHFGP 266
Cdd:cd23739   81 LRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSPLLMNRLEDKALELLEQFTL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 267 NELRKVLVMLAAQSRRSVPLLRAISYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHC 346
Cdd:cd23739  161 EELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADLLSKVPEMKPVLVASL 240

                 ....*....
gi 387912543 347 AKSFALLKW 355
Cdd:cd23739  241 LKSFGQLRW 249

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 387912543  380 LCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFL 451
Cdd:pfam06743   1 LLSLLLPFARLNYLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72

transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted alpha-helical domain N-terminal to FAST kinase-like protein, subdomain 1 (FAST_1); TBRG4 also called cell cycle progression restoration protein 2 or Fas-activated serine-threonine (FAST) kinase domain-containing protein 4, is a regulator of TGFbeta and is implicated in numerous types of cancer. It serves a role in the tumorigenesis of lung cancer via deregulation of DNA damage-inducible transcript 3 (DDIT3), caveolin 1 (CAV1), and ribonucleotide reductase regulatory subunit M2 (RRM2). This domain lies N-terminal to the FAST_1 domain, and is predicted (by AlphaFold) to be alpha-helical, with similarity to structures of members of the 6-hairpin (6 helical hairpin) clan.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 107 DSNQAAMVLIRLSHLLSEKPEDKGLLIQDAHFHQLLCLLNSQIASVWHGTLSKLLGSLYALGIPKASKELQSVEQEVRWR 186
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRAEKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRWR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 187 MRKLKYKHLAFLAESCATLSQEQHSQELLAELLTHLERRWTEIEDSHTLVTVMMKVGHLSEPLMNRLEDKCLELVEHFGP 266
Cdd:cd23739   81 LRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSPLLMNRLEDKALELLEQFTL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 267 NELRKVLVMLAAQSRRSVPLLRAISYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHC 346
Cdd:cd23739  161 EELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADLLSKVPEMKPVLVASL 240

                 ....*....
gi 387912543 347 AKSFALLKW 355
Cdd:cd23739  241 LKSFGQLRW 249

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 387912543  380 LCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFL 451
Cdd:pfam06743   1 LLSLLLPFARLNYLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72

RAP domain; This domain is found in various eukaryotic species, where it is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain. The domain is involved in plant defence in response to bacterial infection.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 387912543  577 WEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDK 631
Cdd:pfam08373   4 DGPSHFYRNSKQLTGSTKLKHRLLSALGYKVISIPYYEWNQLKTKEEKVEYLKKK 58

transforming growth factor beta (TGFbeta) regulator 4 (TBRG4) and similar proteins; predicted alpha-helical domain N-terminal to FAST kinase-like protein, subdomain 1 (FAST_1); TBRG4 also called cell cycle progression restoration protein 2 or Fas-activated serine-threonine (FAST) kinase domain-containing protein 4, is a regulator of TGFbeta and is implicated in numerous types of cancer. It serves a role in the tumorigenesis of lung cancer via deregulation of DNA damage-inducible transcript 3 (DDIT3), caveolin 1 (CAV1), and ribonucleotide reductase regulatory subunit M2 (RRM2). This domain lies N-terminal to the FAST_1 domain, and is predicted (by AlphaFold) to be alpha-helical, with similarity to structures of members of the 6-hairpin (6 helical hairpin) clan.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 107 DSNQAAMVLIRLSHLLSEKPEDKGLLIQDAHFHQLLCLLNSQIASVWHGTLSKLLGSLYALGIPKASKELQSVEQEVRWR 186
Cdd:cd23739    1 SANCAALAVQRLSRLVVEGRAEKDLLLEDPRFEKLCRLAENQISSLSPGSLVDLLRSLLRLGIPPNSRLLQSLENEVRWR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 187 MRKLKYKHLAFLAESCATLSQEQHSQELLAELLTHLERRWTEIEDSHTLVTVMMKVGHLSEPLMNRLEDKCLELVEHFGP 266
Cdd:cd23739   81 LRRLSFRHLASLAESLATLQRAQEQKELLSELLKLLERRWTEIEDPRDLVTLMRIVGHLSPLLMNRLEDKALELLEQFTL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 267 NELRKVLVMLAAQSRRSVPLLRAISYHLVQKPFSLTKDVLLDVAYAYGKLSFHQTQVSQRLATDLLSLMPSLTSGEVAHC 346
Cdd:cd23739  161 EELRRVLLTLAAQKRRSVPLLRALSYHLLQRSDQLSPKQLSDVLYACGKLNFHDTQLLEKIAADLLSKVPEMKPVLVASL 240

                 ....*....
gi 387912543 347 AKSFALLKW 355
Cdd:cd23739  241 LKSFGQLRW 249

FAST kinase-like protein, subdomain 1; This family represents a conserved region of eukaryotic Fas-activated serine/threonine (FAST) kinases (EC:2.7.1.-) that contains several conserved leucine residues. FAST kinase is rapidly activated during Fas-mediated apoptosis, when it phosphorylates TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Note that many family members are hypothetical proteins. This region is often found immediately N-terminal to the FAST kinase-like protein, subdomain 2.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 387912543  380 LCSVLLAFARLNFHPDQEDQFFSLVHEKLGSELPGLEPALQVDLVWALCVLQQAREAELQAVLHPEFHIQFL 451
Cdd:pfam06743   1 LLSLLLPFARLNYLPPNAEEFFEKLESVLESYLSSFSPELLLDLVWSLCVLGRFPEALLNKVLSPDFLQKLL 72

RAP domain; This domain is found in various eukaryotic species, where it is found in proteins that are important in various parasite-host cell interactions. It is thought to be an RNA-binding domain. The domain is involved in plant defence in response to bacterial infection.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 387912543  577 WEFPNFNSRSKDLLGRFVLARRHIVAAGFLIVDVPFYEWLELKSEWQKGAYLKDK 631
Cdd:pfam08373   4 DGPSHFYRNSKQLTGSTKLKHRLLSALGYKVISIPYYEWNQLKTKEEKVEYLKKK 58

RNA-editing substrate-binding complex subunit 6 (RESC6) and related proteins; RESC6 (GRBC6, MRB3010) is a component of the RNA-editing substrate-binding complex (RESC), that is composed of about 20 components and is involved in kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. RESC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and RNA-editing catalytic complex (RECC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing. RESC6 is predicted (by AlphaFold) to adopt an all-helical structure with a disordered region at the N-terminus. The helical structure that is predicted with high confidence resembles Armadillo (ARM)/beta-catenin-like repeats. ARM repeats have been implicated in mediating protein-protein interactions.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 241 KVGHLSEPLMNRLEDKCLELVEHfgpnELRKVLVMLAAQSRRSVPLLRAisyhlvqkpFSLTkdVLLDvayAYGKLSFHQ 320
Cdd:cd23735   57 ADKALDLASLAAVVHACLTLRSE----DLREVIFTAARKLRENAKTLDA---------VSVA--VLLS---AYARTGVHH 118

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 387912543 321 TQVSQRLATDLLSLMPS--LTSGEVAHCAKSFALLKWLSLPLFEAFAQHVLNRAQDITLPHLCSVLLAFARLNFhpdQED 398
Cdd:cd23735  119 DALFEALSDRVVTLAKDgrFPLAHIVNVASALSRVRFRDEALFGVLRDRALRLREQAPPIELATILDAFARLGL---GDE 195

                        170       180
                 ....*....|....*....|...
gi 387912543 399 QFFSLVHEKLGSELPGLePALQV 421
Cdd:cd23735  196 RLFKELAQRALEVLDEM-NAPLV 217