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Conserved domains on  [gi|543583756|ref|NP_001269504|]
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ubiquitin-conjugating enzyme E2 variant 1 isoform h [Homo sapiens]

Protein Classification

ubiquitin-conjugating enzyme family protein( domain architecture ID 439)

ubiquitin-conjugating enzyme family protein similar to ubiquitin-conjugating enzyme E2 that catalyzes the covalent attachment of ubiquitin to other proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
UBCc_UEV super family cl49610
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; ...
 2-86 4.77e-58

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; The family includes ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain and ubiquitin (Ub) E2 variant (UEV) domain. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. E2 is part of the ubiquitin-mediated protein degradation pathway in which a thioester linkage forms between a conserved cysteine and the C-terminus of ubiquitin, and complexes with ubiquitin protein ligase enzymes, E3. This pathway regulates many fundamental cellular processes. There are also other E2s which form thioester linkages without the use of E3s. Several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins) contains the UEV domain, which lacks the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.


The actual alignment was detected with superfamily member cd23807:

Pssm-ID: 483950  Cd Length: 134  Bit Score: 174.21  E-value: 4.77e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLP 81
Cdd:cd23807   50 TVFENRIYSLKIECGENYPDEPPTVRFVTKINLPCVDQSNGVVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLP 129


                 ....*
gi 543583756  82 QPPEG 86
Cdd:cd23807  130 QPPEG 134
 
Name Accession Description Interval E-value
UEV_UBE2V cd23807
ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related ...
 2-86 4.77e-58

ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related proteins; The E2V subfamily includes mammalian ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1/CROC1/UBE2V/UEV1) and variant 2 (UBE2V2/MMS2/UEV2), yeast ubiquitin-conjugating enzyme variant MMS2, plant ubiquitin-conjugating enzyme E2 variant 1A (UEV1A/MMZ1) and variant 1B (UEV1B/MMZ2), and similar proteins. They have no ubiquitin ligase activity on their own. UBE2V1 (also called UEV-1, CROC-1, or TRAF6-regulated IKK activator 1 beta Uev1A) and UBE2V2 (also called DDVit 1, enterocyte differentiation-associated factor 1 (EDAF-1), enterocyte differentiation-promoting factor 1 (EDPF-1), MMS2 homolog, or vitamin D3-inducible protein) form heterodimers with UBE2N, respectively. Saccharomyces cerevisiae UEV MMS2 has a role in the DNA error-free postreplication repair (PRR) pathway. It forms a heterodimer with UBC13. Arabidopsis thaliana UEV1A and UEV1A form heterodimers with UBC35 or UBC36. The heterodimers possess ubiquitin-conjugating enzyme (EC 2.3.2.23) activity, catalyzing the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Members of this subfamily may mediate transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467427  Cd Length: 134  Bit Score: 174.21  E-value: 4.77e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLP 81
Cdd:cd23807   50 TVFENRIYSLKIECGENYPDEPPTVRFVTKINLPCVDQSNGVVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLP 129


                 ....*
gi 543583756  82 QPPEG 86
Cdd:cd23807  130 QPPEG 134
UBCc smart00212
Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for ...
 2-86 5.71e-21

Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. This pathway functions in regulating many fundamental processes required for cell viability.TSG101 is one of several UBC homologues that lacks this active site cysteine.


Pssm-ID: 214562 [Multi-domain]  Cd Length: 145  Bit Score: 80.42  E-value: 5.71e-21
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756     2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNsSNGVVDpraISVL--AKWQNSYSIKVVLQELRRlMMSKENMK 79
Cdd:smart00212  40 TPYEGGVFKLTIEFPEDYPFKPPKVKFITKIYHPNVD-SSGEIC---LDILkqEKWSPALTLETVLLSLQS-LLSEPNPD 114


                   ....*..
gi 543583756    80 LPQPPEG 86
Cdd:smart00212 115 SPLNADA 121
UQ_con pfam00179
Ubiquitin-conjugating enzyme; Proteins destined for proteasome-mediated degradation may be ...
 2-77 2.28e-06

Ubiquitin-conjugating enzyme; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologs. TSG101 is one of several UBC homologs that lacks this active site cysteine.


Pssm-ID: 459701 [Multi-domain]  Cd Length: 139  Bit Score: 42.57  E-value: 2.28e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756    2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI---NMngvnSSNGVVDpraISVLA--KWQNSYSIKVVLQELRRLMMSKE 76
Cdd:pfam00179  39 TPYEGGVFKLSVEFPEDYPFKPPKVKFTTKIyhpNV----DSSGEVC---LDILKdeRWSPALTLEQVLLSIQSLLSEPN 111


                  .
gi 543583756   77 N 77
Cdd:pfam00179 112 P 112
PTZ00390 PTZ00390
ubiquitin-conjugating enzyme; Provisional
 2-76 2.61e-03

ubiquitin-conjugating enzyme; Provisional


Pssm-ID: 240397  Cd Length: 152  Bit Score: 34.78  E-value: 2.61e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVvdprAISVLA-KWQNSYSIKVVLQELRRLMMSKE 76
Cdd:PTZ00390  44 TPYEGGYYKLELFLPEQYPMEPPKVRFLTKIYHPNIDKLGRI----CLDILKdKWSPALQIRTVLLSIQALLSAPE 115
 
Name Accession Description Interval E-value
UEV_UBE2V cd23807
ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related ...
 2-86 4.77e-58

ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related proteins; The E2V subfamily includes mammalian ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1/CROC1/UBE2V/UEV1) and variant 2 (UBE2V2/MMS2/UEV2), yeast ubiquitin-conjugating enzyme variant MMS2, plant ubiquitin-conjugating enzyme E2 variant 1A (UEV1A/MMZ1) and variant 1B (UEV1B/MMZ2), and similar proteins. They have no ubiquitin ligase activity on their own. UBE2V1 (also called UEV-1, CROC-1, or TRAF6-regulated IKK activator 1 beta Uev1A) and UBE2V2 (also called DDVit 1, enterocyte differentiation-associated factor 1 (EDAF-1), enterocyte differentiation-promoting factor 1 (EDPF-1), MMS2 homolog, or vitamin D3-inducible protein) form heterodimers with UBE2N, respectively. Saccharomyces cerevisiae UEV MMS2 has a role in the DNA error-free postreplication repair (PRR) pathway. It forms a heterodimer with UBC13. Arabidopsis thaliana UEV1A and UEV1A form heterodimers with UBC35 or UBC36. The heterodimers possess ubiquitin-conjugating enzyme (EC 2.3.2.23) activity, catalyzing the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Members of this subfamily may mediate transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467427  Cd Length: 134  Bit Score: 174.21  E-value: 4.77e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLP 81
Cdd:cd23807   50 TVFENRIYSLKIECGENYPDEPPTVRFVTKINLPCVDQSNGVVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLP 129


                 ....*
gi 543583756  82 QPPEG 86
Cdd:cd23807  130 QPPEG 134
UBCc smart00212
Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for ...
 2-86 5.71e-21

Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. This pathway functions in regulating many fundamental processes required for cell viability.TSG101 is one of several UBC homologues that lacks this active site cysteine.


Pssm-ID: 214562 [Multi-domain]  Cd Length: 145  Bit Score: 80.42  E-value: 5.71e-21
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756     2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNsSNGVVDpraISVL--AKWQNSYSIKVVLQELRRlMMSKENMK 79
Cdd:smart00212  40 TPYEGGVFKLTIEFPEDYPFKPPKVKFITKIYHPNVD-SSGEIC---LDILkqEKWSPALTLETVLLSLQS-LLSEPNPD 114


                   ....*..
gi 543583756    80 LPQPPEG 86
Cdd:smart00212 115 SPLNADA 121
UBCc_UEV cd00195
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; ...
 1-74 2.44e-13

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; The family includes ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain and ubiquitin (Ub) E2 variant (UEV) domain. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. E2 is part of the ubiquitin-mediated protein degradation pathway in which a thioester linkage forms between a conserved cysteine and the C-terminus of ubiquitin, and complexes with ubiquitin protein ligase enzymes, E3. This pathway regulates many fundamental cellular processes. There are also other E2s which form thioester linkages without the use of E3s. Several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins) contains the UEV domain, which lacks the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.


Pssm-ID: 467407 [Multi-domain]  Cd Length: 112  Bit Score: 60.00  E-value: 2.44e-13
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 543583756   1 MTIYENRIYSLKIECGPKYPEAPPFVRFVTKI-NMNgvNSSNGVVDpraISVL--AKWQNSYSIKVVLQELRRLMMS 74
Cdd:cd00195   39 GTPYEGGVFKLDIEFPDDYPFKPPKVRFLTPIyHPN--VDPDGEIC---LDILksEGWSPALTLRSVLLSIQSLLSD 110
UBCc_ScPEX4-like cd23812
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Saccharomyces cerevisiae Peroxin-4 ...
 2-32 3.13e-08

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Saccharomyces cerevisiae Peroxin-4 (PEX4) protein and related proteins; Saccharomyces cerevisiae PEX4 (EC 2.3.2.23), also called ubiquitin-conjugating enzyme E2-21 kDa, UBC10, or PAS2, acts as a ubiquitin-conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. It is essential for peroxisome biogenesis and is required for UBC4-independent ubiquitination of PEX5. This subfamily also includes Arabidopsis thaliana PEX4 (also known as UBC21, EC 2.3.2.23) that is required for peroxisome biogenesis. It is necessary for the developmental elimination of obsolete peroxisome matrix proteins. It may be involved in the ubiquitination of PEX5, targeting it for recycling.


Pssm-ID: 467432 [Multi-domain]  Cd Length: 145  Bit Score: 47.54  E-value: 3.13e-08
                         10        20        30
                 ....*....|....*....|....*....|.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI 32
Cdd:cd23812   42 TPYEGGRFELAIQVPSNYPISPPKVKFVTKI 72
UBCc_ApmR795-like cd23833
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Acanthamoeba polyphaga mimivirus ...
 2-76 5.76e-07

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Acanthamoeba polyphaga mimivirus bifunctional E2/E3 enzyme R795 and related proteins; R795 (EC 2.3.2.23/EC 2.3.2.27) is a bifunctional enzyme which acts as an E2 ubiquitin-conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. It also acts as a RING-type E3 ubiquitin-protein transferase.


Pssm-ID: 467438 [Multi-domain]  Cd Length: 117  Bit Score: 43.76  E-value: 5.76e-07
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDpraiSVLAK-WQNSYSIKVVLQELRRLMMSKE 76
Cdd:cd23833   40 TPYEGGVFLLYVEFPEEYPVKPPEVRFITPIYHCNINSDGRICH----SILDRnYTPDTTMREILDAVYGLLLTPE 111
UBCc_UBE2T cd23805
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 T ...
 2-72 1.74e-06

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 T and related enzymes; The E2T subfamily includes mammalian ubiquitin-conjugating enzymes E2 T (UBE2T/HSPC150/PIG50), plant ubiquitin-conjugating enzyme E2 37 (UBC37), and similar proteins. They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2T, also called cell proliferation-inducing gene 50 protein, catalyzes monoubiquitination. It is involved in mitomycin-C (MMC)-induced DNA repair. It acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. UBE2T also mediates monoubiquitination of FANCL and FANCI. It may contribute to ubiquitination and degradation of BRCA1. In vitro, UBE2T can promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.


Pssm-ID: 467425  Cd Length: 146  Bit Score: 42.90  E-value: 1.74e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI---NMNgvnsSNGVVdprAISVL-----AKWQNSYSIKVVLQELRRLM 72
Cdd:cd23805   40 TPYEGGVFKLEITIPERYPFEPPKVRFLTPIyhpNID----SAGRI---CLDILkmppkGSWKPSLNISTVLTSIRLLL 111
UBCc_UBE2K cd23800
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 K ...
 2-87 1.99e-06

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 K and related proteins; The E2J subfamily includes mammalian ubiquitin-conjugating enzymes E2 K (UBE2K/HIP2/LIG), yeast ubiquitin-conjugating enzyme E2 1 (UBC1), and plant ubiquitin-conjugating enzyme E2 27 (UBC27). They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2K is also called huntingtin-interacting protein 2 (HIP-2), ubiquitin-conjugating enzyme E2-25 kDa, or ubiquitin-conjugating enzyme E2(25K). In vitro, in the presence or absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, UBE2K catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. It does not transfer ubiquitin directly, but elongates monoubiquitinated substrate proteins. Saccharomyces cerevisiae UBC1, also called ubiquitin-conjugating enzyme E2-24 kDa, functions in the degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. It is a cognate E2 conjugating enzyme for the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M).


Pssm-ID: 467420  Cd Length: 145  Bit Score: 42.93  E-value: 1.99e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGvvdprAI--SVLA-KWQNSYSIKVVLQELRRLMMSkenm 78
Cdd:cd23800   43 TPYEGGTFVLDIKIPDTYPFEPPKMKFITKIWHPNISSQTG-----AIclDILKdQWSPALTLRTALLSIQALLSA---- 113


                 ....*....
gi 543583756  79 klPQPPEGQ 87
Cdd:cd23800  114 --PEPDDPQ 120
UQ_con pfam00179
Ubiquitin-conjugating enzyme; Proteins destined for proteasome-mediated degradation may be ...
 2-77 2.28e-06

Ubiquitin-conjugating enzyme; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologs. TSG101 is one of several UBC homologs that lacks this active site cysteine.


Pssm-ID: 459701 [Multi-domain]  Cd Length: 139  Bit Score: 42.57  E-value: 2.28e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 543583756    2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI---NMngvnSSNGVVDpraISVLA--KWQNSYSIKVVLQELRRLMMSKE 76
Cdd:pfam00179  39 TPYEGGVFKLSVEFPEDYPFKPPKVKFTTKIyhpNV----DSSGEVC---LDILKdeRWSPALTLEQVLLSIQSLLSEPN 111


                  .
gi 543583756   77 N 77
Cdd:pfam00179 112 P 112
UBCc_SpUBC14-like cd23815
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Schizosaccharomyces pombe UBC14 ...
 2-72 3.18e-06

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Schizosaccharomyces pombe UBC14 and related proteins; Schizosaccharomyces pombe UBC14 (EC 2.3.2.23), also called ubiquitin-conjugating enzyme E2 14, E2 ubiquitin-conjugating enzyme 14, ubiquitin carrier protein 14, or ubiquitin-protein ligase 14, acts as a ubiquitin-conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. It mediates the selective degradation of short-lived and abnormal proteins.


Pssm-ID: 467435  Cd Length: 143  Bit Score: 42.28  E-value: 3.18e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVvdprAISVLAK--WQNSYSIKVVLQELRRLM 72
Cdd:cd23815   40 SPYEGGIFKFKITFPEDYPFKPPTVKFTTKIYHPNVDDDGSI----CLGILKSdaWKPSIKLVSVLNALLDLL 108
UBCc_UBE2S cd23804
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 S ...
 2-72 2.88e-05

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 S and related domains; The E2S subfamily includes mammalian ubiquitin-conjugating enzymes E2 S (UBE2S/E2EPF), plant ubiquitin-conjugating enzyme E2 22 (UBC22), and similar proteins. They are ubiquitin-conjugating enzymes (EC2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2S catalyzes 'Lys-11'-linked polyubiquitination. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. UBE2S acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. It also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. UBE2S is also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro, it can promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.


Pssm-ID: 467424  Cd Length: 146  Bit Score: 39.77  E-value: 2.88e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI---NMngvnSSNGVVdprAISVLAK-WQNSYSIKVVLQELRRLM 72
Cdd:cd23804   44 TPYEGGVFRVKLVLGPDFPASPPKGYFLTKIfhpNV----SPTGEI---CVNTLKKdWKPELGLRHILLTIRCLL 111
UBCc_UBE2A_2B cd23790
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzymes E2A, ...
 2-32 7.44e-05

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzymes E2A, E2B and related proteins; The E2A/2B subfamily includes mammalian ubiquitin-conjugating enzymes UBE2A/RAD6A and UBE2B/RAD6B, yeast ubiquitin-conjugating enzyme E2 2 (UBC2/RAD6), plant ubiquitin-conjugating enzyme E2 1-3 (UBC1-3), and similar proteins. They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. Both UBE2A/RAD6A and UBE2B/RAD6B are required for post-replication repair of UV-damaged DNA. In vitro, they catalyze 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. UBE2B might also catalyze 'Lys-63'-linked polyubiquitination. Saccharomyces cerevisiae UBC2 is required for DNA repair, damage-induced mutagenesis, and sporulation.


Pssm-ID: 467410  Cd Length: 143  Bit Score: 38.64  E-value: 7.44e-05
                         10        20        30
                 ....*....|....*....|....*....|.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI 32
Cdd:cd23790   44 TPWEGGTFKLRLEFSEEYPNKPPKVRFVSKM 74
UBCc_UBE2C cd23791
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 C ...
 2-72 3.43e-04

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 C and related proteins; The E2C family includes mammalian ubiquitin-conjugating enzyme E2 C (UBE2C/UBCH10), yeast E2 ubiquitin-conjugating enzyme 11 (UBC11), plant ubiquitin-conjugating enzyme E2 19 (UBC19) and 20 (UBC20). They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2C, also known as (E3-independent) E2 ubiquitin-conjugating enzyme C (EC 2.3.2.24), E2 ubiquitin-conjugating enzyme C, ubiquitin carrier protein C, or ubiquitin-protein ligase C, catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination in vitro. It is a ubiquitin carrier protein required for the destruction of mitotic cyclins and proteins that maintain sister chromatid cohesion in animal cells and in Schizosaccharomyces pombe. In Saccharomyces cerevisiae, UBC11 is not essential for mitotic cyclin destruction. Arabidopsis thaliana UBC19 is part of the anaphase-promoting complex (APC). It may have a key function during cell cycle and be involved in cyclin B1 degradation.


Pssm-ID: 467411  Cd Length: 140  Bit Score: 36.78  E-value: 3.43e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI---NmngvnssngvVDPRAISVL----AKWQNSYSIKVVLQELRRLM 72
Cdd:cd23791   41 TVYEGLKYKLSLEFPSNYPYKAPTVKFETPCfhpN----------VDQHGNICLdilkEKWSALYDVRTILLSIQSLL 108
UBCc_UBE2E cd23793
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 ...
 2-72 6.35e-04

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 E1-E3 and related proteins; The E2E subfamily includes mammalian ubiquitin-conjugating enzyme E2 E1-3 (UBE2E1/UBCH6, UBE2E2/UBCH8, UBE2E3/UBCH9) and similar proteins. UBE2E, also known as (E3-independent) E2 ubiquitin-conjugating enzyme E, or E2 ubiquitin-conjugating enzyme E, accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2E1 (EC 2.3.2.23 and EC 2.3.2.24) catalyzes the covalent attachment of ISG15 to other proteins. It mediates the selective degradation of short-lived and abnormal proteins. In vitro, it also catalyzes 'Lys-48'-linked polyubiquitination. In vitro, both UBE2E2 (EC 2.3.2.23) and UBE2E3 (EC 2.3.2.23) catalyze 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. UBE2E2 catalyzes the ISGylation of influenza A virus NS1 protein. UBE2E3 participates in the regulation of trans-epithelial sodium transport in renal cells. It may be involved in cell growth arrest.


Pssm-ID: 467413  Cd Length: 141  Bit Score: 36.20  E-value: 6.35e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSnGVVdprAISVLA-KWQNSYSIKVVLQELRRLM 72
Cdd:cd23793   40 SVYEGGVFFLDIHFPPDYPFKPPKVTFRTRIYHCNINSQ-GVI---CLDILKdNWSPALTISKVLLSICSLL 107
UEV_AKTIP cd23814
ubiquitin E2 variant (UEV) domain of AKT-interacting protein and related proteins; AKTIP, ...
 3-70 6.90e-04

ubiquitin E2 variant (UEV) domain of AKT-interacting protein and related proteins; AKTIP, also called Ft1, or fused toes protein homolog, is a component of the FTS/Hook/FHIP complex (FHF complex), which may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). AKTIP regulates apoptosis by enhancing phosphorylation and activation of AKT1. It increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. AKTIP contains a UEV domain that is homologous to E2 ubiquitin ligases but lacks the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467434  Cd Length: 112  Bit Score: 35.60  E-value: 6.90e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 543583756   3 IYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRaiSVLAKWQ-NSYSIKVVLQELRR 70
Cdd:cd23814   40 LYKGGIFRFTISIPDNYPDGPPRVTFLSPVFHPLVDPQTGELDLS--RAFPKWRpGKHHIWHVLNYLKR 106
UEV_Morgue-like cd23826
ubiquitin E2 variant (UEV) domain of Drosophila melanogaster Morgue and related proteins; ...
 2-32 1.18e-03

ubiquitin E2 variant (UEV) domain of Drosophila melanogaster Morgue and related proteins; Morgue is an F-box/ubiquitin conjugase domain protein important for grim-reaper mediated apoptosis. It contains both an F-box and a UEV domain that is homologous to E2 ubiquitin ligases but lacks the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467436 [Multi-domain]  Cd Length: 147  Bit Score: 35.29  E-value: 1.18e-03
                         10        20        30
                 ....*....|....*....|....*....|.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKI 32
Cdd:cd23826   44 SPYEGGIFFLRIQIPESYPFRPPKVRFLTKI 74
UBCc_UBE2W cd23808
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 W ...
 2-29 2.60e-03

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 W and related enzymes; The E2W subfamily includes mammalian ubiquitin-conjugating enzymes E2 W (UBE2W/UBC16), plant ubiquitin-conjugating enzyme E2 15-18 (UBC15-18), and similar proteins. They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. UBE2W, also called FLJ11011, E2 ubiquitin-conjugating enzyme W, N-terminal E2 ubiquitin-conjugating enzyme (EC 2.3.2.25), N-terminus-conjugating E2, ubiquitin carrier protein W, ubiquitin-conjugating enzyme 16 (UBC-16), or ubiquitin-protein ligase W, specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini. In vitro, UBE2W catalyzes 'Lys-11'-linked polyubiquitination. UBE2W is an important protein for early postnatal survival and for the normal functioning of multiple organ systems.


Pssm-ID: 467428 [Multi-domain]  Cd Length: 119  Bit Score: 34.04  E-value: 2.60e-03
                         10        20
                 ....*....|....*....|....*...
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFV 29
Cdd:cd23808   41 TLYEGEKFRLRFKFPPDYPIESPEVVFV 68
PTZ00390 PTZ00390
ubiquitin-conjugating enzyme; Provisional
 2-76 2.61e-03

ubiquitin-conjugating enzyme; Provisional


Pssm-ID: 240397  Cd Length: 152  Bit Score: 34.78  E-value: 2.61e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 543583756   2 TIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVvdprAISVLA-KWQNSYSIKVVLQELRRLMMSKE 76
Cdd:PTZ00390  44 TPYEGGYYKLELFLPEQYPMEPPKVRFLTKIYHPNIDKLGRI----CLDILKdKWSPALQIRTVLLSIQALLSAPE 115
UBCc_UBE2N cd23813
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 N ...
 4-32 3.68e-03

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 N and related proteins; The E2N subfamily includes mammalian ubiquitin-conjugating enzymes E2 N (UBE2N/UBCH13/UBC13/BLU), yeast ubiquitin-conjugating enzyme E2 13 (UBC13), and plant ubiquitin-conjugating enzyme E2 35-36 (UBC35/UBC13A/UBG13A, UBC36/UBC13B/UBG13B), which function as ubiquitin-conjugating enzymes (EC 2.3.2.23). UBE2N, also called Bendless-like ubiquitin-conjugating enzyme, forms heterodimers with UBE2V1 and UBE2V2, respectively. The UBE2V1/UBE2N and UBE2V2/UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. UBE2N also plays a role in the control of progress through the cell cycle and differentiation, as well as in the error-free DNA repair pathway, and contributes to the survival of cells after DNA damage. Saccharomyces cerevisiae UBC13 has a role in the DNA error-free post-replication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. Arabidopsis thaliana UBC35 and UBC36 catalyze the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. They mediate transcriptional activation of target genes. They are required for post-replication repair of UV-damaged DNA and for adapting root developmental programs to suboptimal availability of iron.


Pssm-ID: 467433  Cd Length: 144  Bit Score: 34.09  E-value: 3.68e-03
                         10        20
                 ....*....|....*....|....*....
gi 543583756   4 YENRIYSLKIECGPKYPEAPPFVRFVTKI 32
Cdd:cd23813   44 YEGGVFKLELFLPEEYPMAPPKVRFLTKI 72
UBCc_UBE2D cd23792
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 ...
 4-44 8.63e-03

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin conjugating enzyme E2 D1-D3 and related proteins; The E2D family includes mammalian ubiquitin-conjugating enzyme E2 D1-4 (UBE2D1/SFT/UBC5A/UBCH5/UBCH5A, UBE2D2/PUBC1/UBC4/UBC5B/UBCH4/UBCH5B, UBE2D3/UBC5C/UBCH5C, UBE2D4/HBUCE1/UBCH5D), yeast E2 ubiquitin-conjugating enzyme 4 (UBC4) and 5 (UBC5), as well as plant counterpart ubiquitin-conjugating enzyme E2 8-12 (UBC8/UBCAT4A, UBC9/UBCAT4B, UBC10-12) and 28-30 (UBC28-30). They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. In vitro, UBE2D1-3 (EC 2.3.2.23 and EC 2.3.2.24) catalyze 'Lys-48'-linked polyubiquitination. UBE2D3 also catalyzes 'Lys-11'-linked polyubiquitination. In vitro, UBE2D4 can promote polyubiquitination using all 7 ubiquitin Lys residues but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Saccharomyces cerevisiae UBC4-5 and Arabidopsis thaliana UBC8-11 mediates the selective degradation of short-lived and abnormal proteins.


Pssm-ID: 467412  Cd Length: 143  Bit Score: 33.00  E-value: 8.63e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 543583756   4 YENRIYSLKIECGPKYPEAPPFVRFVTKI---NMNgvnsSNGVV 44
Cdd:cd23792   43 YQGGVFFLNIHFPTDYPFKPPKVAFTTKIyhpNIN----SNGSI 82
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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