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Conserved domains on  [gi|1007380412|ref|NP_001308090|]
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tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform 6 [Homo sapiens]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 10614797)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor

CATH:  2.20.25.110
EC:  2.1.-.-|2.1.1.-
Gene Ontology:  GO:0032259|GO:0008757|GO:0008168|GO:1904047
PubMed:  12504684|12826405

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 155-241 5.20e-08

Methyltransferase domain; This family appears to be a methyltransferase domain.


:

Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 49.48  E-value: 5.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 155 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 234
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72


                  ....*..
gi 1007380412 235 LSSIHPD 241
Cdd:pfam13649  73 LHHLPDP 79
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 155-241 5.20e-08

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 49.48  E-value: 5.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 155 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 234
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72


                  ....*..
gi 1007380412 235 LSSIHPD 241
Cdd:pfam13649  73 LHHLPDP 79
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 154-244 7.21e-07

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 47.30  E-value: 7.21e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 154 RILEVGCGAGNSVFPILNTlenspESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDGILDVILLVF 233
Cdd:COG2226    25 RVLDLGCGTGRLALALAER-----GARVTGVDISPEMLELARE----RAAEAGLNVEFVVGDAEDLPFPDGSFDLVISSF 95

                          90
                  ....*....|..
gi 1007380412 234 VLSSI-HPDRTL 244
Cdd:COG2226    96 VLHHLpDPERAL 107
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 154-246 3.56e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 41.65  E-value: 3.56e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 154 RILEVGCGAGNsvfpILNTLENSPESFLYCCDFASGAVELVKSHSSYRATQCFAFVHdvCDDGLPYPFPDGILDVILLVF 233
Cdd:cd02440     1 RVLDLGCGTGA----LALALASGPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDP 74

                          90
                  ....*....|...
gi 1007380412 234 VLSSIHPDRTLFI 246
Cdd:cd02440    75 PLHHLVEDLARFL 87
PRK08317 PRK08317
hypothetical protein; Provisional
 145-244 5.26e-05

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 43.00  E-value: 5.26e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 145 LFPGSnatfRILEVGCGAGNSVFPILNtlENSPESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDG 224
Cdd:PRK08317   17 VQPGD----RVLDVGCGPGNDARELAR--RVGPEGRVVGIDRSEAMLALAKE----RAAGLGPNVEFVRGDADGLPFPDG 86

                          90       100
                  ....*....|....*....|.
gi 1007380412 225 ILDVILLVFVLSSIH-PDRTL 244
Cdd:PRK08317   87 SFDAVRSDRVLQHLEdPARAL 107
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 155-241 5.20e-08

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 49.48  E-value: 5.20e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 155 ILEVGCGAGNSVFPILNTLENSpesfLYCCDFASGAVELVKSHSSYRATQCFAFVHDVCDdglpYPFPDGILDVILLVFV 234
Cdd:pfam13649   1 VLDLGCGTGRLTLALARRGGAR----VTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGV 72


                  ....*..
gi 1007380412 235 LSSIHPD 241
Cdd:pfam13649  73 LHHLPDP 79
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 154-244 7.21e-07

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 47.30  E-value: 7.21e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 154 RILEVGCGAGNSVFPILNTlenspESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDGILDVILLVF 233
Cdd:COG2226    25 RVLDLGCGTGRLALALAER-----GARVTGVDISPEMLELARE----RAAEAGLNVEFVVGDAEDLPFPDGSFDLVISSF 95

                          90
                  ....*....|..
gi 1007380412 234 VLSSI-HPDRTL 244
Cdd:COG2226    96 VLHHLpDPERAL 107
Methyltransf_12 pfam08242
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 156-244 8.94e-07

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 400515 [Multi-domain]  Cd Length: 98  Bit Score: 46.21  E-value: 8.94e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 156 LEVGCGAGNSVFPILNTLensPESFLYCCDFASGAVELVKSH-SSYRATQCFAFVHDVCDDGLPYPFPdgiLDVILLVFV 234
Cdd:pfam08242   1 LEIGCGTGTLLRALLEAL---PGLEYTGLDISPAALEAARERlAALGLLNAVRVELFQLDLGELDPGS---FDVVVASNV 74

                          90
                  ....*....|.
gi 1007380412 235 LSSI-HPDRTL 244
Cdd:pfam08242  75 LHHLaDPRAVL 85
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 154-246 3.56e-05

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 41.65  E-value: 3.56e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 154 RILEVGCGAGNsvfpILNTLENSPESFLYCCDFASGAVELVKSHSSYRATQCFAFVHdvCDDGLPYPFPDGILDVILLVF 233
Cdd:cd02440     1 RVLDLGCGTGA----LALALASGPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDP 74

                          90
                  ....*....|...
gi 1007380412 234 VLSSIHPDRTLFI 246
Cdd:cd02440    75 PLHHLVEDLARFL 87
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 156-244 3.74e-05

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 41.50  E-value: 3.74e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 156 LEVGCGAGNSVFPILNTLENspesfLYCCDFASGAVELVKSHSSYRAtqcfafVHDVCDDGLPYPFPDGILDVILLVFVL 235
Cdd:pfam08241   1 LDVGCGTGLLTELLARLGAR-----VTGVDISPEMLELAREKAPREG------LTFVVGDAEDLPFPDNSFDLVLSSEVL 69

                          90
                  ....*....|
gi 1007380412 236 SSIH-PDRTL 244
Cdd:pfam08241  70 HHVEdPERAL 79
PRK08317 PRK08317
hypothetical protein; Provisional
 145-244 5.26e-05

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 43.00  E-value: 5.26e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 145 LFPGSnatfRILEVGCGAGNSVFPILNtlENSPESFLYCCDFASGAVELVKShssyRATQCFAFVHDVCDDGLPYPFPDG 224
Cdd:PRK08317   17 VQPGD----RVLDVGCGPGNDARELAR--RVGPEGRVVGIDRSEAMLALAKE----RAAGLGPNVEFVRGDADGLPFPDG 86

                          90       100
                  ....*....|....*....|.
gi 1007380412 225 ILDVILLVFVLSSIH-PDRTL 244
Cdd:PRK08317   87 SFDAVRSDRVLQHLEdPARAL 107
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 145-242 1.96e-04

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 41.06  E-value: 1.96e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1007380412 145 LFPGSNATFRILEVGCGAGNSVFPILNTLenspESFLYCCDFASGAVEL-VKSHSSYRATQCFAFVHDVCDdglPYPFPD 223
Cdd:COG0500    20 LLERLPKGGRVLDLGCGTGRNLLALAARF----GGRVIGIDLSPEAIALaRARAAKAGLGNVEFLVADLAE---LDPLPA 92

                          90
                  ....*....|....*....
gi 1007380412 224 GILDVILLVFVLSSIHPDR 242
Cdd:COG0500    93 ESFDLVVAFGVLHHLPPEE 111
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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