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Conserved domains on  [gi|1036551133|ref|NP_001315333|]
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E3 SUMO-protein ligase EGR2a isoform 1 [Danio rerio]

Protein Classification

DUF3446 and COG5048 domain-containing protein( domain architecture ID 12112558)

protein containing domains DUF3446, zf-C2H2, and COG5048

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DUF3446 pfam11928
Early growth response N-terminal domain; Proteins containing this domain include early growth ...
 91-182 4.90e-12

Early growth response N-terminal domain; Proteins containing this domain include early growth response protein 1/2/3 (EGR1/2/3). EGR1 is a transcriptional regulator that binds specifically to 9-bp target sequences containing two CpG sites that can potentially be methylated at four cytosine bases. EGR2, also known as Krox20, is an E3 ligase that sumoylates its Nab coregulators. This domain has a single completely conserved residue P that may be functionally important. Furthermore, it is found associated with pfam00096.


:

Pssm-ID: 463400  Cd Length: 104  Bit Score: 62.16  E-value: 4.90e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133  91 GKISIDAQ----GSWSQEGIINFISAGVQDRSTSPGSFS-----TGSPAGPPSDCSKIVQNLPN-MEHMYTGPPPYTCSA 160
Cdd:pfam11928   1 GKFSIDPQypggGSLWPEGLISLVSGLVGMANPPSSSSSsspssSSSPNSSGPLCTMSCSVQSSeMEHIYSAPPPYPSSG 80

                          90       100
                  ....*....|....*....|....
gi 1036551133 161 EG--YQDPSVYLSTTTCPITYAPP 182
Cdd:pfam11928  81 CGdlYQDPSAFLSTPSGPPLAYPP 104
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
321-382 1.59e-06

FOG: Zn-finger [General function prediction only];


:

Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.08  E-value: 1.59e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1036551133 321 KPFQCRICMRSFSRSDHLTTHIRTHTGEKPFSCDFCGRKFARSD--ERRRHTKIHQRHKDRKLS 382
Cdd:COG5048    32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRplELSRHLRTHHNNPSDLNS 95
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 293-317 3.40e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


:

Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.58  E-value: 3.40e-03
                          10        20
                  ....*....|....*....|....*
gi 1036551133 293 YPCPaeGCDRRFSRSDELTRHVRIH 317
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
 
Name Accession Description Interval E-value
DUF3446 pfam11928
Early growth response N-terminal domain; Proteins containing this domain include early growth ...
 91-182 4.90e-12

Early growth response N-terminal domain; Proteins containing this domain include early growth response protein 1/2/3 (EGR1/2/3). EGR1 is a transcriptional regulator that binds specifically to 9-bp target sequences containing two CpG sites that can potentially be methylated at four cytosine bases. EGR2, also known as Krox20, is an E3 ligase that sumoylates its Nab coregulators. This domain has a single completely conserved residue P that may be functionally important. Furthermore, it is found associated with pfam00096.


Pssm-ID: 463400  Cd Length: 104  Bit Score: 62.16  E-value: 4.90e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133  91 GKISIDAQ----GSWSQEGIINFISAGVQDRSTSPGSFS-----TGSPAGPPSDCSKIVQNLPN-MEHMYTGPPPYTCSA 160
Cdd:pfam11928   1 GKFSIDPQypggGSLWPEGLISLVSGLVGMANPPSSSSSsspssSSSPNSSGPLCTMSCSVQSSeMEHIYSAPPPYPSSG 80

                          90       100
                  ....*....|....*....|....
gi 1036551133 161 EG--YQDPSVYLSTTTCPITYAPP 182
Cdd:pfam11928  81 CGdlYQDPSAFLSTPSGPPLAYPP 104
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
321-382 1.59e-06

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.08  E-value: 1.59e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1036551133 321 KPFQCRICMRSFSRSDHLTTHIRTHTGEKPFSCDFCGRKFARSD--ERRRHTKIHQRHKDRKLS 382
Cdd:COG5048    32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRplELSRHLRTHHNNPSDLNS 95
zf-H2C2_2 pfam13465
Zinc-finger double domain;
337-362 4.16e-06

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 43.13  E-value: 4.16e-06
                          10        20
                  ....*....|....*....|....*.
gi 1036551133 337 HLTTHIRTHTGEKPFSCDFCGRKFAR 362
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 293-317 3.40e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.58  E-value: 3.40e-03
                          10        20
                  ....*....|....*....|....*
gi 1036551133 293 YPCPaeGCDRRFSRSDELTRHVRIH 317
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
PHA00733 PHA00733
hypothetical protein
 322-369 9.32e-03

hypothetical protein


Pssm-ID: 177301  Cd Length: 128  Bit Score: 36.01  E-value: 9.32e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1036551133 322 PFQCRICMRSFSRSDHLTTHIRTHTGEKpfSCDFCGRKFARSDERRRH 369
Cdd:PHA00733   73 PYVCPLCLMPFSSSVSLKQHIRYTEHSK--VCPVCGKEFRNTDSTLDH 118
 
Name Accession Description Interval E-value
DUF3446 pfam11928
Early growth response N-terminal domain; Proteins containing this domain include early growth ...
 91-182 4.90e-12

Early growth response N-terminal domain; Proteins containing this domain include early growth response protein 1/2/3 (EGR1/2/3). EGR1 is a transcriptional regulator that binds specifically to 9-bp target sequences containing two CpG sites that can potentially be methylated at four cytosine bases. EGR2, also known as Krox20, is an E3 ligase that sumoylates its Nab coregulators. This domain has a single completely conserved residue P that may be functionally important. Furthermore, it is found associated with pfam00096.


Pssm-ID: 463400  Cd Length: 104  Bit Score: 62.16  E-value: 4.90e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133  91 GKISIDAQ----GSWSQEGIINFISAGVQDRSTSPGSFS-----TGSPAGPPSDCSKIVQNLPN-MEHMYTGPPPYTCSA 160
Cdd:pfam11928   1 GKFSIDPQypggGSLWPEGLISLVSGLVGMANPPSSSSSsspssSSSPNSSGPLCTMSCSVQSSeMEHIYSAPPPYPSSG 80

                          90       100
                  ....*....|....*....|....
gi 1036551133 161 EG--YQDPSVYLSTTTCPITYAPP 182
Cdd:pfam11928  81 CGdlYQDPSAFLSTPSGPPLAYPP 104
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
321-382 1.59e-06

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 50.08  E-value: 1.59e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1036551133 321 KPFQCRICMRSFSRSDHLTTHIRTHTGEKPFSCDFCGRKFARSD--ERRRHTKIHQRHKDRKLS 382
Cdd:COG5048    32 RPDSCPNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRplELSRHLRTHHNNPSDLNS 95
zf-H2C2_2 pfam13465
Zinc-finger double domain;
337-362 4.16e-06

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 43.13  E-value: 4.16e-06
                          10        20
                  ....*....|....*....|....*.
gi 1036551133 337 HLTTHIRTHTGEKPFSCDFCGRKFAR 362
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-H2C2_2 pfam13465
Zinc-finger double domain;
309-334 2.18e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.82  E-value: 2.18e-05
                          10        20
                  ....*....|....*....|....*.
gi 1036551133 309 ELTRHVRIHTGHKPFQCRICMRSFSR 334
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
SFP1 COG5189
Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division ...
 278-376 3.17e-05

Putative transcriptional repressor regulating G2/M transition [Transcription / Cell division and chromosome partitioning];


Pssm-ID: 227516 [Multi-domain]  Cd Length: 423  Bit Score: 45.86  E-value: 3.17e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 278 KYPNRPCKTpVHERPYPCPAEGCDRRFSRSDELTRHvRIHtGHK-----PFQCRICMRSFSRSDhltthirthtgeKPFS 352
Cdd:COG5189   336 DTPSRMLKV-KDGKPYKCPVEGCNKKYKNQNGLKYH-MLH-GHQnqklhENPSPEKMNIFSAKD------------KPYR 400

                          90       100
                  ....*....|....*....|....
gi 1036551133 353 CDFCGRKFARSDERRRHTKiHQRH 376
Cdd:COG5189   401 CEVCDKRYKNLNGLKYHRK-HSHD 423
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
225-393 8.04e-04

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 41.22  E-value: 8.04e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 225 FSCSLESIRVPPPLTPLNTIRNFTLACPVDGSRPPLDGTNPQNVPLRPILRPRkYPNRPCKTPVHERPYPCPAEGCDRRF 304
Cdd:COG5048   192 VSTSIPSSSENSPLSSSYSIPSSSSDQNLENSSSSLPLTTNSQLSPKSLLSQS-PSSLSSSDSSSSASESPRSSLPTASS 270

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 305 SRSDELTRHVRIHTG-HKPFQCRICMRSFSRSDHLTTHIRT--HTGE--KPFSCDF--CGRKFARSDERRRHTKIHQRHK 377
Cdd:COG5048   271 QSSSPNESDSSSEKGfSLPIKSKQCNISFSRSSPLTRHLRSvnHSGEslKPFSCPYslCGKLFSRNDALKRHILLHTSIS 350

                         170
                  ....*....|....*.
gi 1036551133 378 DRKLSVGPHSSSAGGN 393
Cdd:COG5048   351 PAKEKLLNSSSKFSPL 366
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 323-345 8.68e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 36.51  E-value: 8.68e-04
                          10        20
                  ....*....|....*....|...
gi 1036551133 323 FQCRICMRSFSRSDHLTTHIRTH 345
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
289-354 1.11e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 40.83  E-value: 1.11e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1036551133 289 HERPYPCPaeGCDRRFSRSDELTRHVRIHTGHKPFQCRICMR--SFSRSDHLTTHIRTHTGEKPFSCD 354
Cdd:COG5048    30 APRPDSCP--NCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCdkSFSRPLELSRHLRTHHNNPSDLNS 95
COG5236 COG5236
Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];
295-375 2.58e-03

Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only];


Pssm-ID: 227561 [Multi-domain]  Cd Length: 493  Bit Score: 40.00  E-value: 2.58e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 295 CPAEGCDRRFSRSDELTRHVRIHtgHKPFQCRICMR---------SFSRSDHLTTHIRTHTGEKPFS----CDFCGRKFA 361
Cdd:COG5236   154 CPKSKCHRRCGSLKELKKHYKAQ--HGFVLCSECIGnkkdfwneiRLFRSSTLRDHKNGGLEEEGFKghplCIFCKIYFY 231

                          90
                  ....*....|....*
gi 1036551133 362 RSDERRRHTKI-HQR 375
Cdd:COG5236   232 DDDELRRHCRLrHEA 246
COG5048 COG5048
FOG: Zn-finger [General function prediction only];
218-389 3.17e-03

FOG: Zn-finger [General function prediction only];


Pssm-ID: 227381 [Multi-domain]  Cd Length: 467  Bit Score: 39.68  E-value: 3.17e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 218 VFQDIKPFSCSLESIRVPPPLTPLNTIRNFTLacPVDGSRPPLDGTNPQNVPLRPILRPRKYPNRPCKTPVHERP----- 292
Cdd:COG5048   206 SSSYSIPSSSSDQNLENSSSSLPLTTNSQLSP--KSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESdssse 283

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1036551133 293 ----YPCPAEGCDRRFSRSDELTRHVR--IHTG--HKPFQCRI--CMRSFSRSDHLTTHIRTHTGEKPFSCDF--CGRKF 360
Cdd:COG5048   284 kgfsLPIKSKQCNISFSRSSPLTRHLRsvNHSGesLKPFSCPYslCGKLFSRNDALKRHILLHTSISPAKEKLlnSSSKF 363

                         170       180       190
                  ....*....|....*....|....*....|.
gi 1036551133 361 ARSDERRRHTKIHQRH--KDRKLSVGPHSSS 389
Cdd:COG5048   364 SPLLNNEPPQSLQQYKdlKNDKKSETLSNSC 394
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 293-317 3.40e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.58  E-value: 3.40e-03
                          10        20
                  ....*....|....*....|....*
gi 1036551133 293 YPCPaeGCDRRFSRSDELTRHVRIH 317
Cdd:pfam00096   1 YKCP--DCGKSFSRKSNLKRHLRTH 23
zf-C2H2_4 pfam13894
C2H2-type zinc finger; This family contains a number of divergent C2H2 type zinc fingers.
 323-345 5.59e-03

C2H2-type zinc finger; This family contains a number of divergent C2H2 type zinc fingers.


Pssm-ID: 464025  Cd Length: 24  Bit Score: 34.16  E-value: 5.59e-03
                          10        20
                  ....*....|....*....|...
gi 1036551133 323 FQCRICMRSFSRSDHLTTHIRTH 345
Cdd:pfam13894   1 FKCPICGKSFSSKKSLKRHLKTH 23
PHA00733 PHA00733
hypothetical protein
 322-369 9.32e-03

hypothetical protein


Pssm-ID: 177301  Cd Length: 128  Bit Score: 36.01  E-value: 9.32e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1036551133 322 PFQCRICMRSFSRSDHLTTHIRTHTGEKpfSCDFCGRKFARSDERRRH 369
Cdd:PHA00733   73 PYVCPLCLMPFSSSVSLKQHIRYTEHSK--VCPVCGKEFRNTDSTLDH 118
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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