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Conserved domains on  [gi|1169100874|ref|NP_001336750|]
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sodium-dependent lysophosphatidylcholine symporter 1 isoform 6 [Homo sapiens]

Protein Classification

sodium-dependent lysophosphatidylcholine symporter 1( domain architecture ID 13024589)

sodium-dependent lysophosphatidylcholine symporter 1 (NLS1) plays an essential role for blood-brain barrier formation and function

CATH:  1.20.1250.20
Gene Ontology:  GO:0051978|GO:0051977|GO:0016020
PubMed:  23177091|26098515|26758938
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 44-494 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 341009  Cd Length: 419  Bit Score: 744.33  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 123
Cdd:cd17451     1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 203
Cdd:cd17451    81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 204 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 283
Cdd:cd17451   161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 284 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 363
Cdd:cd17451   209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 364 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17451   289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 444 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17451   369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
 
Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 44-494 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341009  Cd Length: 419  Bit Score: 744.33  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 123
Cdd:cd17451     1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 203
Cdd:cd17451    81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 204 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 283
Cdd:cd17451   161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 284 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 363
Cdd:cd17451   209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 364 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17451   289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 444 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17451   369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 46-494 5.64e-96

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 298.07  E-value: 5.64e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  46 CYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTcLGRLMPWIIFSTPLAV 125
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTR-WGRRRPWLLLSAIILA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 126 IAYFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVG 204
Cdd:pfam13347  80 VSFILLFTPPELgRAPLFIWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAFPLVL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 205 qadtpcfqdlnsstvasqsanhthGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVRE---QREPYEAQQSEPIAYFR 281
Cdd:pfam13347 160 ------------------------ILGGTGLERKGYRIFALIGAVLMLLGVIITAAGTKErvsMRSKEDTGQKEAGSLLD 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 282 GLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVG 361
Cdd:pfam13347 216 MLKEVFRNRAFLILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAASAFPLVFTIGALLGIPLWPPLAKRIGKKNTYILG 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 362 ISSAVPFLILVALM-ESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEpiFFSFYVFFTKFASGVS 440
Cdd:pfam13347 296 ALITIAGFALALLLgPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGT--FFAMWSFISKLATGVG 373

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1169100874 441 LGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:pfam13347 374 LGVSGLLLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
39-510 1.89e-76

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 247.89  E-value: 1.89e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWII 118
Cdd:COG2211     7 LSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGA-LSDRTRTRWGRRRPWIL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 119 FSTPLAVIAYFLIWFVPDFPHGQTYWYLL-FYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTA 197
Cdd:COG2211    86 IGAIPLALSFVLLFTAPDLSPTGKLIYALvTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFAGLGGLLASV 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 198 IQGQIVGQADTpcfqdlnsstvasqsanhthgttshrETQKAYLLAAGVIVCIYIICAVILILGVREQREPyeaqQSEPI 277
Cdd:COG2211   166 LPPPLVAAFGG--------------------------DAALGYRLTALIFAVLGLLAFLLTFFGTKERPVP----EEEKV 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 278 AYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKT 356
Cdd:COG2211   216 SLKESLKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLsAALVGLLLALYFLAALLGAPLWPRLAKRFGKKK 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 357 AVYVGIS-SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKF 435
Cdd:COG2211   296 AFIIGLLlAALGLLLLFFLGPGNLWLLLVLAALAGIGLGAILVLPWAMLADVVDYDEWK--TGRRREGLYFGIFTFAIKL 373

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1169100874 436 ASGVSLGISTLSLDFAGYQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDE 510
Cdd:COG2211   374 GQALAGALAGLLLALFGYVA-GAAQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARRAA 447
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 44-499 8.96e-30

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 121.59  E-value: 8.96e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPWI-IFS 120
Cdd:TIGR00792   1 KLSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVdrTRTRW---GKFRPWLlIGA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 121 TPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMtvevlgtvLGTAIQ 199
Cdd:TIGR00792  78 IPFSIVL-VLLFTTPDFsGTGKLVYAYITYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRR--------FGATLG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 200 GQIVGQADTPcfqdlnsSTVASQSANHTHGttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAY 279
Cdd:TIGR00792 149 GLLVAVIVLP-------LVSYFGGGDDKFG----------WFMFALVLALIGVVSLIICFFGTKERYSEIPKNIEKKLSL 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 280 FRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVY 359
Cdd:TIGR00792 212 KQIFKALFKNDQLLILCLAYLFYNLAFNIKNGVQVYYFTYVLGDPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRKILFA 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 360 VGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPhfHGTEPIFFSFYVFFTKFASGV 439
Cdd:TIGR00792 292 GGILLMVLGYLIFFFAGSNLPLILVLIILAGFGQNIVTGLVWALVADTVDYGEWKTG--IRAEGLVYSVRTFVRKLGQAL 369

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 440 SLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLF-KMYPIDEERRRQ 499
Cdd:TIGR00792 370 AGFFVGLILGIIGY-VANAAQSPITLNGIKILMFAVPALFLLLAAIIIgRFYKLTEKKHAE 429
PRK09669 PRK09669
putative symporter YagG; Provisional
 39-496 2.24e-17

putative symporter YagG; Provisional


Pssm-ID: 236610  Cd Length: 444  Bit Score: 84.39  E-value: 2.24e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGapyqvTGCAL-----GFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGRL 113
Cdd:PRK09669    6 LTTKDKIGYGLGD-----TACNLvwqtvMLFLAYFYTDVFGLSAAIMGTMFLVVRVLDAVTDPLMGALVDRTR-TRHGQF 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 114 MPWII-FSTPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLG 191
Cdd:PRK09669   80 RPYLLwFAIPFGVVC-LLTFYTPDFgATGKIIYACVTYILLSLVYTAINVPYCAMPGAITNDPRERHSLQSWRFALSFIG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 192 TVLGTAIQGQIV---GQADtpcfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQREP 268
Cdd:PRK09669  159 GLIVSVIALPLVdilGKGD----------------------------EQKGYFYAMMVMGLLGVVLFFCCFFMTKERYTP 210

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 269 YEAQQSepiAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWF 348
Cdd:PRK09669  211 EVDNSS---SVWKDLKLLLGNSQWRIMFIFNVVLLTAVVTRGGATLYYVNYVLLRPDLATLFLVTGMIAGLFGALLSERL 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 349 LTRFGKKTAVYVGISSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfHLKQPHFHGTEPIFFS 427
Cdd:PRK09669  288 LGKFDRVRAFKWTIVAFVILSALIFFIPpSNVWLIFALNILFNFIQNLTTPLQWSMFSDVVD--YEEKRSGRRLDGLVFS 365

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 428 FYVFFTKFasGVSLGISTL--SLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEER 496
Cdd:PRK09669  366 TNLFAIKL--GLAIGGAVVgwILAWVDYVGGAAVQSASVLTTINLLFTVIPGVLFAGMAIILNFYKLNDAR 434
 
Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 44-494 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341009  Cd Length: 419  Bit Score: 744.33  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 123
Cdd:cd17451     1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 203
Cdd:cd17451    81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 204 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 283
Cdd:cd17451   161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 284 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 363
Cdd:cd17451   209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 364 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17451   289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 444 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17451   369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
MFS_MFSD2 cd17392
Major facilitator superfamily domain-containing protein 2 subfamily; The major facilitator ...
 44-494 0e+00

Major facilitator superfamily domain-containing protein 2 subfamily; The major facilitator superfamily domain-containing protein 2 (MFSD2) subfamily is composed of two vertebrate members, MFSD2A amd MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. The MFSD2 subfamily belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340950  Cd Length: 446  Bit Score: 739.67  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 123
Cdd:cd17392     1 KLCYAIGGAPYQMTSSATGFFLQIFLLDVAQMGAFSVSLILFVGRVWDAVTDPLVGYFISRSQRTGIGRLMPWIVFSTPL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 203
Cdd:cd17392    81 IILAYFFLWFLPPFTSLSGLWYLTFYCLFETFMTCFHVPYSALTMFLGGCQRERDSATAYRMTVEVFGTLMGATIQGQIV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 204 GQADTPCFQDLNSSTVASqsanhTHGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 283
Cdd:cd17392   161 GVAHRPRRQDCTATPGTS-----DHPVTVLPNTRRAYLIAALVVVVLYFVCCSILFLGVKEQPDPLSPASGPGLSYLAGL 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 284 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 363
Cdd:cd17392   236 KLVTGHPPYLKLVIGFLFSSLAFQMEQGNFVLFCTHAAGLGDHFQHLVLAILVSATLSIPLWQWVLQRRGKKTTSFIGIS 315

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 364 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17392   316 AMVPFLILLALVPSNLPVAYVVAVVSGVSLAALFLLPWSMLPDVVDDFQLKNPHCPGLEPIFYSCYVFFTKLGGGLSLGI 395

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 444 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17392   396 STLSLHFSGYKAGACKQPEEVVVTLKVLIAPVPICLILAGLVLFYVYPINE 446
MFS_MFSD2B cd17452
Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily ...
 44-494 0e+00

Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily domain-containing protein 2B (MFSD2B) is closely related to MFSD2A, and their conserved genomic structure suggests that they are derived from the duplication of an ancestral gene. Variations of chromosome 2 gene expressions among patients with lung cancer or non-cancer identified MFSD2B as a potential risk or protect factor in the prognosis of lung adenocarcinoma. MFSD2B belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341010  Cd Length: 416  Bit Score: 544.41  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 123
Cdd:cd17452     1 KLCYAIGGAPNQVAGSATAFFLQIYLLDIAQITPFQASLVLFVGKAWGAATDPVVGFFISKSKWTKIGRLMPWMLGCTPF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 203
Cdd:cd17452    81 IVVSYFFLWFVPPFTTGRFLWYLTFYCLFQALATCFHVPYSALTMFLSTDQRERDSATAYRMTVEVLGTLVGAAVQGQIV 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 204 GQAdtpcfqdlnsstvasqsanhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 283
Cdd:cd17452   161 ASA---------------------------------YMIAAGVIGGLYLLCISVLFLGVKERDDPYAPKSGKAIPFFKGL 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 284 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAvYVGIS 363
Cdd:cd17452   208 SLTMRHGPYLKLTASFLFISAAVQLEQSNFVLFCTHAVDLHDHFQNLVLTILVSAVVSIPFWQWFLQRFGKKAA-ACGIS 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 364 SAVPFLILVALMeSNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17452   287 WMIPFAIMLVTI-PNLIVAYVVAFVSGLSIAASLLLPWSMLPDVVDDFRLQNPHGKGLETIFYSSYVFFTKLSAGIALGI 365

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 444 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17452   366 STLSLEFAGYETGACKQPESVVLTLKLLIGAAPTCMILIGLCILMFYPITE 416
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 44-494 6.14e-102

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 313.00  E-value: 6.14e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGRLMPWIIFSTPL 123
Cdd:cd17332     1 KIGYGLGDFGNNLIFQIVSTYLLFFYTDVLGLSAAAAGTIFLVARIIDAINDPLMGYLIDRTR-SRWGRFRPWLLWGAIP 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 124 AVIAYFLIWFVPDFPH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQI 202
Cdd:cd17332    80 LALLFVLLFTTPDGSGtGKLIYALITYILLDLLYTLVNIPYTALIPELTDDPEERTSLTSWRMFFATIGGLLVTVLPPPL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 203 VGQADTPCfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPiAYFRG 282
Cdd:cd17332   160 VAYFGGGN-------------------------ASRGYFLTALIIGIIGIILLLICFFGTRERVVPPEEEKSKL-PLLKS 213

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 283 LRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGI 362
Cdd:cd17332   214 LKALLKNRPFLILLLAYLLYFLAFNIVNTVLVYYFKYVLGGRAELVLLLLLILSGALLALLPWPPLKKRFGKKKAFFIGL 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 363 SSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKFASGVSL 441
Cdd:cd17332   294 LLAILGLLLLFFLPpGNLVLILVLAVLAGIGYGGANLLPWAMLADVIDYGELK--TGKRREGIFYSVMTFFRKLGLALAG 371

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1169100874 442 GISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:cd17332   372 ALVGLILSLAGYVANAAAQSASALNGIRLLIAVLPAVLLLLALILMSFYPLDK 424
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 46-494 5.64e-96

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 298.07  E-value: 5.64e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  46 CYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTcLGRLMPWIIFSTPLAV 125
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTR-WGRRRPWLLLSAIILA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 126 IAYFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVG 204
Cdd:pfam13347  80 VSFILLFTPPELgRAPLFIWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAFPLVL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 205 qadtpcfqdlnsstvasqsanhthGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVRE---QREPYEAQQSEPIAYFR 281
Cdd:pfam13347 160 ------------------------ILGGTGLERKGYRIFALIGAVLMLLGVIITAAGTKErvsMRSKEDTGQKEAGSLLD 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 282 GLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVG 361
Cdd:pfam13347 216 MLKEVFRNRAFLILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAASAFPLVFTIGALLGIPLWPPLAKRIGKKNTYILG 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 362 ISSAVPFLILVALM-ESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEpiFFSFYVFFTKFASGVS 440
Cdd:pfam13347 296 ALITIAGFALALLLgPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGT--FFAMWSFISKLATGVG 373

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1169100874 441 LGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 494
Cdd:pfam13347 374 LGVSGLLLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
39-510 1.89e-76

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 247.89  E-value: 1.89e-76
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWII 118
Cdd:COG2211     7 LSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGA-LSDRTRTRWGRRRPWIL 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 119 FSTPLAVIAYFLIWFVPDFPHGQTYWYLL-FYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTA 197
Cdd:COG2211    86 IGAIPLALSFVLLFTAPDLSPTGKLIYALvTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFAGLGGLLASV 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 198 IQGQIVGQADTpcfqdlnsstvasqsanhthgttshrETQKAYLLAAGVIVCIYIICAVILILGVREQREPyeaqQSEPI 277
Cdd:COG2211   166 LPPPLVAAFGG--------------------------DAALGYRLTALIFAVLGLLAFLLTFFGTKERPVP----EEEKV 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 278 AYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKT 356
Cdd:COG2211   216 SLKESLKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLsAALVGLLLALYFLAALLGAPLWPRLAKRFGKKK 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 357 AVYVGIS-SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKF 435
Cdd:COG2211   296 AFIIGLLlAALGLLLLFFLGPGNLWLLLVLAALAGIGLGAILVLPWAMLADVVDYDEWK--TGRRREGLYFGIFTFAIKL 373

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1169100874 436 ASGVSLGISTLSLDFAGYQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDE 510
Cdd:COG2211   374 GQALAGALAGLLLALFGYVA-GAAQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARRAA 447
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 44-499 8.96e-30

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 121.59  E-value: 8.96e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  44 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPWI-IFS 120
Cdd:TIGR00792   1 KLSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVdrTRTRW---GKFRPWLlIGA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 121 TPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMtvevlgtvLGTAIQ 199
Cdd:TIGR00792  78 IPFSIVL-VLLFTTPDFsGTGKLVYAYITYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRR--------FGATLG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 200 GQIVGQADTPcfqdlnsSTVASQSANHTHGttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAY 279
Cdd:TIGR00792 149 GLLVAVIVLP-------LVSYFGGGDDKFG----------WFMFALVLALIGVVSLIICFFGTKERYSEIPKNIEKKLSL 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 280 FRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVY 359
Cdd:TIGR00792 212 KQIFKALFKNDQLLILCLAYLFYNLAFNIKNGVQVYYFTYVLGDPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRKILFA 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 360 VGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPhfHGTEPIFFSFYVFFTKFASGV 439
Cdd:TIGR00792 292 GGILLMVLGYLIFFFAGSNLPLILVLIILAGFGQNIVTGLVWALVADTVDYGEWKTG--IRAEGLVYSVRTFVRKLGQAL 369

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 440 SLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLF-KMYPIDEERRRQ 499
Cdd:TIGR00792 370 AGFFVGLILGIIGY-VANAAQSPITLNGIKILMFAVPALFLLLAAIIIgRFYKLTEKKHAE 429
PRK09669 PRK09669
putative symporter YagG; Provisional
 39-496 2.24e-17

putative symporter YagG; Provisional


Pssm-ID: 236610  Cd Length: 444  Bit Score: 84.39  E-value: 2.24e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGapyqvTGCAL-----GFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGRL 113
Cdd:PRK09669    6 LTTKDKIGYGLGD-----TACNLvwqtvMLFLAYFYTDVFGLSAAIMGTMFLVVRVLDAVTDPLMGALVDRTR-TRHGQF 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 114 MPWII-FSTPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLG 191
Cdd:PRK09669   80 RPYLLwFAIPFGVVC-LLTFYTPDFgATGKIIYACVTYILLSLVYTAINVPYCAMPGAITNDPRERHSLQSWRFALSFIG 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 192 TVLGTAIQGQIV---GQADtpcfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQREP 268
Cdd:PRK09669  159 GLIVSVIALPLVdilGKGD----------------------------EQKGYFYAMMVMGLLGVVLFFCCFFMTKERYTP 210

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 269 YEAQQSepiAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWF 348
Cdd:PRK09669  211 EVDNSS---SVWKDLKLLLGNSQWRIMFIFNVVLLTAVVTRGGATLYYVNYVLLRPDLATLFLVTGMIAGLFGALLSERL 287

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 349 LTRFGKKTAVYVGISSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfHLKQPHFHGTEPIFFS 427
Cdd:PRK09669  288 LGKFDRVRAFKWTIVAFVILSALIFFIPpSNVWLIFALNILFNFIQNLTTPLQWSMFSDVVD--YEEKRSGRRLDGLVFS 365

                         410       420       430       440       450       460       470
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1169100874 428 FYVFFTKFasGVSLGISTL--SLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEER 496
Cdd:PRK09669  366 TNLFAIKL--GLAIGGAVVgwILAWVDYVGGAAVQSASVLTTINLLFTVIPGVLFAGMAIILNFYKLNDAR 434
PRK10429 PRK10429
melibiose:sodium transporter MelB;
39-526 9.15e-13

melibiose:sodium transporter MelB;


Pssm-ID: 182453  Cd Length: 473  Bit Score: 70.51  E-value: 9.15e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPW 116
Cdd:PRK10429    3 ISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVnnTRSRW---GKFKPW 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 117 IIFSTPLAVIAYFLIWFVPDFPHGQTYWYL-LFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLG 195
Cdd:PRK10429   80 ILIGTLANSVVLFLLFSAHLFEGTAQYVFVcVTYILWGMTYTIMDIPFWSLVPTLTLDKREREQLVPYPRFFASLAGFVT 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 196 TAIQGQIVGQadtpcfqdlnsstvasqsanhthgtTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSE 275
Cdd:PRK10429  160 AGFTLPFVNY-------------------------VGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVYSSDNQVSAE 214

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 276 PIAY-FRG-LRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFG 353
Cdd:PRK10429  215 GSHLtLKDiVALIYKNDQLSCLLGMALAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLVTLILFPRLVKSLS 294

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 354 KKTaVYVGISSaVPFLILVALMesnLIITYAVAVAAGISVAAAFL-----LPWS----MLPDVID--DFHLKQPhfhgTE 422
Cdd:PRK10429  295 RRI-LWAGASI-FPVLSCGVLL---LMGLAAPHNALLIVIAGILLnigtaLFWVlqviMVADTVDygEYKLGIR----CE 365

                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 423 PIFFSFYVFFTKFASGVSLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLL-FKMYPIDEERRRQNK 501
Cdd:PRK10429  366 SIAYSVQTMVVKGGSAFAAFFIGVVLGLIGY-VPNVEQSAQTLLGMQFIMIGLPTLFFMITLVLyFRYYRLNGDFLRRIQ 444

                         490       500
                  ....*....|....*....|....*
gi 1169100874 502 KALQALRDEASSSGCSETDSTELAS 526
Cdd:PRK10429  445 IHLLDKYRKVPPEPVHAEIPVGAVS 469
PRK09848 PRK09848
glucuronide transporter; Provisional
 64-511 2.42e-11

glucuronide transporter; Provisional


Pssm-ID: 182109 [Multi-domain]  Cd Length: 448  Bit Score: 65.96  E-value: 2.42e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  64 FLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSpWTCLGRLMPWIIF-STPLAVIAYFLIWFVPDFPHGQT 142
Cdd:PRK09848   30 FLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSV-NTRWGKFRPFLLFgTAPLMIFSVLVFWVPTDWSHSSK 108

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 143 YWY-LLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRM-----TVEVLGTVLGTAIQgqivgqadtpcfqdlnS 216
Cdd:PRK09848  109 VVYaYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGiaaslTFVCLAFLIGPSIK----------------N 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 217 STVASQSANHTHGTtshretqkayLLAAGVIVCIYIICavililgVREQREPYEAQQSEPiAYFRGLRLVMSHGPYIKLI 296
Cdd:PRK09848  173 SSPEEMVSVYHFWT----------IVLAIAGMVLYFIC-------FKSTRENVVRIVAQP-SLKISLQTLKRNRPLFMLC 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 297 TGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATL-TIPIWQWFLTRFGKKTAVYVGIS-SAVPFLILVAL 374
Cdd:PRK09848  235 IGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVGTVaSAPLVPGMVARIGKKNTFLIGALlGTCGYLLFFWV 314

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 375 MESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQphfhG--TEPIFFSFYVFFTKFASGVSLGISTLSLDFAG 452
Cdd:PRK09848  315 SVWSLPVALVALAIASIGQGVTMTVMWALEADTVEYGEYLT----GvrIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSG 390

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1169100874 453 YQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDEA 511
Cdd:PRK09848  391 YIA-NQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ 448
PRK11462 PRK11462
putative transporter; Provisional
 39-203 2.98e-10

putative transporter; Provisional


Pssm-ID: 183145  Cd Length: 460  Bit Score: 62.26  E-value: 2.98e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  39 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPW 116
Cdd:PRK11462    6 LSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPAGFVGTMFLVARALDAISDPCMGLLAdrTRSRW---GKFRPW 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 117 IIF-STPLAVIAyFLIWFVPDFPH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVL 194
Cdd:PRK11462   83 VLFgALPFGIVC-VLAYSTPDLSMnGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGML 161


                  ....*....
gi 1169100874 195 GTAIQGQIV 203
Cdd:PRK11462  162 STVLMMPLV 170
MFS_MFSD12 cd17491
Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily ...
 64-440 2.28e-07

Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily domain-containing protein 12 (MFSD12) protein subfamily includes a group of uncharacterized proteins similar to human MFSD2. MFSD2 is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. The MFSD12 subfamily belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341044  Cd Length: 438  Bit Score: 53.03  E-value: 2.28e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  64 FLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPW-TCLGRLMPWIIFSTPLAVIAYFLIW-----FVPDF 137
Cdd:cd17491    21 YLLVYFHLVLGFSSALAGILLLIGQVADAISTPLVGYESDRTNGcGKYGRRKSWHLIGTICVLLSFPFIFnpclgCSDST 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 138 PH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYR--MTVEVLGTVLGTA--IQGQIVGQADTPcfq 212
Cdd:cd17491   101 SEwAKLVYYGPFIIIFQFGWAAVQISHLSLIPELTSDEHERVELTALRyaFTVIANITVYLIAwlLLQQSSGDSVNP--- 177

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 213 dlNSSTVASQSANhthgttshretqkayllAAGVIVCIYIICAVILILGVREQREP--YEAQQSEPIAYFRGLRLVMSHG 290
Cdd:cd17491   178 --TSAQDLPIFRN-----------------LSLIVVGIGFLFSLLFHLGTKEPRPPtdLSTTTNEGEEVTAPEEPRLTWK 238

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 291 PYIKL----ITGFLFTS--LAFMLVEGNFVLFCTYTLGFRNEFQNLL-LAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 363
Cdd:cd17491   239 EWLREpqfyQVALLYMCtrLIVNLSQVYIPLYLTETLQLPKESIAIVpLVMYVSGFLTSLVMKPINKKIGRKITYLLGLL 318

                         330       340       350       360       370       380       390
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1169100874 364 SAVPFLILVAL--MESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfhlkqpHFHGTEPIFFSFYVFFTKFASGVS 440
Cdd:cd17491   319 FVLGFCIWVWFqsGSSRTYEIYGAAVLLGVGSATLLVTSLSMTADLIG-------TNTESGAFVYGAMSFTDKLSNGIA 390
MFS_SLC45_SUC cd17313
Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily ...
 45-443 9.19e-06

Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily of transporters; This group includes the solute carrier 45 (SLC45) family as well as plant sucrose transporters (SUCs or SUTs) and similar proteins such as Schizosaccharomyces pombe general alpha-glucoside permease. the SLC45 family is composed of four (A1-A4) vertebrate proteins as well as related insect proteins such as Drosophila sucrose transporter SCRT or Slc45-1. Members of this group transport sucrose and other sugars like maltose into the cell, with the concomitant uptake of protons (symport system). Plant sucrose transporters are crucial to carbon partitioning, playing a key role in phloem loading/unloading. They play a key role in loading and unloading of sucrose into the phloem and as a result, they control sucrose distribution throughout the whole plant and drive the osmotic flow system in the phloem. They also play a role in the exchange of sucrose between beneficial symbionts (mycorrhiza and Rhizobium) as well as pathogens such as nematodes and parasitic fungi. There are nine sucrose transporter genes in Arabidopsis and five in rice. Vertebrate SLC45 family proteins have been implicated in the regulation of glucose homoeostasis in the brain (SLC45A1), with skin and hair pigmentation (SLC45A2), and with prostate cancer and myelination (SLC45A3). Mutations in SLC45A2, also called MATP (membrane-associated transporter protein) or melanoma antigen AIM1, cause oculocutaneous albinism type 4 (OCA4), an autosomal recessive disorder of melanin biosynthesis that results in congenital hypopigmentation of ocular and cutaneous tissues. The SLC45 family and related sugar transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340871 [Multi-domain]  Cd Length: 421  Bit Score: 48.01  E-value: 9.19e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  45 LCYALGGApyqvtgcalgfFLQIYLLDVAqVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWIIFSTPLA 124
Cdd:cd17313    13 FGWALENS-----------YVPPILQTLG-LSHALIGFVWTLGPILGLFVQPLVGS-LSDRCNSRIGRRRPFILVGAPLA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 125 VIAYFLIWFVPDFPHG---QTYW-----YLLFYCLFETMVTCFHVPYSALtMFISTEQTERDSATAYRMTVEVLGTVLGT 196
Cdd:cd17313    80 ALGLILIPNAADIGLAlgdGPRTwalvlFVLGIVLLDFAMNVCQGPVRAL-LPDLVPPEQRSKANGIINFMGGLGGVLGY 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 197 AIQGQivgqadtpcfqdLNSSTVAsqsanhthGTTSHRETQKAYLLAAGVIVciyiICAVILILGVREQR----EPYEAQ 272
Cdd:cd17313   159 LLGAI------------LWDHNWF--------GFALGGNLKVPFYIGAIILL----VCVLVTLFFVKEPPlspeAGEHSE 214

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 273 QSEPIAYFRGLRLVMSHGP--YIKLITGFLFTSLAFMLVEGNFVLFCT-----------YTLGFRNEFQNLLLAImLSAT 339
Cdd:cd17313   215 NPSLGEVLKSLLKLLKIMPrsLLRLLLVIFFWWIAFFPFELFFTDYMGeevyhgtsaasSHYGAGVRMGAWGLLI-FSLA 293

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 340 LTI---PIwQWFLTRFGKKtAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQp 416
Cdd:cd17313   294 FLIfslPI-GKLGKKIGRK-KVYLIGLVLFAVGMALMALVHNVTVALVLFALGGIGWATININPYPLVSDYHSESGRGQ- 370

                         410       420
                  ....*....|....*....|....*..
gi 1169100874 417 hfhGTEPIFFSFYVFFTKFASGVSLGI 443
Cdd:cd17313   371 ---GTDTGLLNLAISLAQILVSLLAGP 394
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 240-397 2.69e-05

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 46.42  E-value: 2.69e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 240 YLLAAGVIVCIYIICAVILILG-------VREQREPYEAQQSEPIAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGN 312
Cdd:cd17325   142 LLADALGYRAPFLVCAALALLAlvlalllLPEPRPPPNKRAVSAARLRSGLRLLLRDRRLLALFLAIFVLAFAFGALEPF 221

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 313 FVLFCTYTLGFrNEFQN--LLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMeSNLIITYAVAVAAG 390
Cdd:cd17325   222 LPLYAAELGGL-SPAQIglLFGAQGLASALSQPPAGKLSDRIGRKPLILIGLLLSAVALLLLPLA-TSFWLLLLLLALLG 299


                  ....*..
gi 1169100874 391 ISVAAAF 397
Cdd:cd17325   300 LGLGLVF 306
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
243-407 1.98e-04

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 43.72  E-value: 1.98e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 243 AAGVIVCIYIICAVILILGVREqrEPYEAQQSEPIAyFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLG 322
Cdd:COG2223   161 AFLILGILLLVVAVLAWLFLRD--PPAGAAAAAKAS-LRDQLEALRDPRFWLLSLLYFGTFGSFIGFSSWLPPYLVDQFG 237

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 323 FRNEFQNLLLAIM-LSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPW 401
Cdd:COG2223   238 LSAATAGLLAALFaLLGALGRPLGGWLSDRIGGRRVLLIVFALMALGLLLLALALGSLWLFLVLFLLLGLALGGGNGAVF 317


                  ....*.
gi 1169100874 402 SMLPDV 407
Cdd:COG2223   318 ALVPDI 323
MFS_FsR cd17478
Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters; ...
 63-397 5.90e-04

Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters; Fosmidomycin resistance protein (FsR) confers resistance against fosmidomycin. It shows sequence similarity with the bacterial drug-export proteins that mediate resistance to tetracycline and chloramphenicol. This FsR family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341031 [Multi-domain]  Cd Length: 365  Bit Score: 42.16  E-value: 5.90e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  63 FFLQIYLLDVAQVGpfsasIILFVGRAWDAITDPLVGLCISKSPWtclGRLMPWIIFSTPLAVIayfLIWFVPDFphgqt 142
Cdd:cd17478    21 LLIAKLGLSYAQAG-----LLVSVFSITSSLLQPLFGYLADRKPR---RYLLPLGPLLTGLGLS---LLGFAPNY----- 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 143 YWYLLFYCLFETMVTCFHVPYSALTMFISTEQteRDSATAYRMTVEVLGTVLGTAIQGQIVgqadtpcfqdlnsstvasq 222
Cdd:cd17478    85 WLLLLLVALAGIGSAAFHPEGARLASQAAGGR--RGLAMSIFSVGGNLGFALGPLIAAALV------------------- 143

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 223 sanhthgttshretQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGLRLVMshGPYIKLITGFLFT 302
Cdd:cd17478   144 --------------ALFGGLGLLILLIIPGLAVALLLLRLRPLKKPEAKKAAAKSSARSTLKGVW--RALLLLLIIVFLR 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 303 SLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALmeSNLIIT 382
Cdd:cd17478   208 SWVLSSLTTFLPFYLVQRGYSLVFAGLLLSLFLFAGAIGTLIGGPLSDRFGRRRVIIASLLLAAPFLLLFLL--TPGIWS 285

                         330
                  ....*....|....*
gi 1169100874 383 YAVAVAAGISVAAAF 397
Cdd:cd17478   286 YILLVLAGFLLFASF 300
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
117-407 9.58e-04

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 41.50  E-value: 9.58e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 117 IIFSTPLAVIAYFLIWFVPDFPhgqtyWYLLFYCLFETMVTCFHVPYSALTMFISTEQtERDSATAYRMTVEVLGTVLGT 196
Cdd:COG2814    78 LLLGLLLFALGSLLCALAPSLW-----LLLAARFLQGLGAGALFPAALALIADLVPPE-RRGRALGLLGAGLGLGPALGP 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 197 AIQGQIVGQADtpcfqdlnsstvasqsanhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSep 276
Cdd:COG2814   152 LLGGLLADLFG--------------------------------WRWVFLVNAVLALLALLLLLRLLPESRPAARARLR-- 197

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 277 iayfRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKK 355
Cdd:COG2814   198 ----GSLRELLRRPRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLsASAAGLLLALFGLGGVLGALLAGRLADRFGRR 273

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1169100874 356 TAVYVGISSAVPFLILVALMeSNLIITYAVAVAAGISVAAAFLLPWSMLPDV 407
Cdd:COG2814   274 RLLLIGLLLLALGLLLLALA-GSLWLLLLALFLLGFGFGLLFPLLQALVAEL 324
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 64-403 2.94e-03

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 40.10  E-value: 2.94e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874  64 FLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKspwtcLGRLmPWIIFSTPLAVIAYFLIWFVPDFphgqtY 143
Cdd:cd06174    17 PLLPALLQSFGLSASQLGLLFALFSLGYALLQPLAGLLADR-----FGRR-PVLLLGLLLFALGALLFAFAPSF-----W 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 144 WYLLFYCLFETMVTCFHVPYSALTMFiSTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVGQADTPcfqdlnsstvasqs 223
Cdd:cd06174    86 LLLLGRFLLGLGSGLIDPAVLALIAD-LFPERERGRALGLLQAFGSVGGILGPLLGGILASSLGFG-------------- 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 224 anhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIA--YFRGLRLVMSHGPYIKLITGFLF 301
Cdd:cd06174   151 ----------------WRAVFLIAAALALLAAILLLLVVPDPPESARAKNEEASSksVLKLLKRVLKNPGLWLLLLAIFL 214

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 302 TSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAI-MLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLI 380
Cdd:cd06174   215 VNLAYYSFSTLLPLFLLDLGGLSVAVAGLLLSLfGLAGALGSLLLGLLSDRLIGRKPLLLIGLLLMALGLALLLLAPSLL 294

                         330       340
                  ....*....|....*....|...
gi 1169100874 381 ITYAVAVAAGISVAAAFLLPWSM 403
Cdd:cd06174   295 LLLLLLLLLGFGLGGLLPLSFAL 317
BtlA COG2270
MFS-type transporter involved in bile tolerance, Atg22 family [General function prediction ...
 111-437 5.70e-03

MFS-type transporter involved in bile tolerance, Atg22 family [General function prediction only];


Pssm-ID: 441871 [Multi-domain]  Cd Length: 424  Bit Score: 39.37  E-value: 5.70e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 111 GRLMPWIIFSTPLAVIAYFLIWFVPDfphGQTYWYLLFYCL----FETMVtcfhVPYSALTMFISTEQtERD--SATAYR 184
Cdd:COG2270    80 GRKKPFLAFFTLLGVLATAALFFVAP---GGLWLGLLLFVLanigFEGSL----VFYNAFLPDIATPE-EIGrvSGLGWA 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 185 M----TVEVLGTVLGTAIQGQIVGQADTpcfqdlnsstvasqsanhthgttsHRETQKAYLLAAGVivciYIICAVILIL 260
Cdd:COG2270   152 LgylgGVLLLLINLALFLKPEAFGLPDA------------------------GEAVRISFLLTALW----WLLFALPLFL 203

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 261 GVREQREPYEAQQSEPIAY-FRGLRLVMSHGPYIKLITGFLftsLAFML----VEGNFVLFCTY---TLGFrnEFQNLLL 332
Cdd:COG2270   204 FLPERPRPGPAPPRGAVRAgFRELRRTLRELRRYRDLLRFL---LAYFFyrdgVQTVIAFAGIYaagVLGF--STTELIV 278

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1169100874 333 AIMLSATLTIP---IWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIItYAVAVAAGISVAAAFLLPWSMLPDVId 409
Cdd:COG2270   279 FGLLVQIVAALgalLFGRLDDRIGPKRVILVSLVIWIVVCLAAYFLTTALAF-WILGLLIGLVMGGIQALSRSLLARLI- 356

                         330       340
                  ....*....|....*....|....*...
gi 1169100874 410 dfhlkqPHFHGTEpiFFSFYVFFTKFAS 437
Cdd:COG2270   357 ------PPGREAE--FFGLYALSGKAAS 376
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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