|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
56-423 |
1.35e-170 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 483.13 E-value: 1.35e-170
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 56 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVT 135
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEDLTPFRDMSKKVY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 136 ELLEEFSP--VVERLGFDENFVDLTEMvekrlqqlqsdelsavtvsghvynnqsinlldvlhiRLLVGSQIAAEMREAMY 213
Cdd:cd01703 81 RLLRSYSWndRVERLGFDENFMDVTEM------------------------------------RLLVASHIAYEMRERIE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 214 NQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSP 291
Cdd:cd01703 125 NELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPPSCADLMdfMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 292 ---------------KILEKELGISVAQRIQKLSFGEDNSPVI-LSGPPQSFSEEDSFKKCSSEV--EAKNKIEELLASL 353
Cdd:cd01703 205 rnrqtvgaapsllelLLMVKEFGEGIGQRIWKLLFGRDTSPVKpASDFPQQISIEDSYKKCSLEEirEARNKIEELLASL 284
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1192727312 354 LNRVCQ--------DGRKPHTVRLIIRRYSSEKH-YGRESRQCPIPSHVIQKLGTGlqspdfcaSSLMQRRLEDKLVKL 423
Cdd:cd01703 285 LERMKQdlqevkagDGRRPHTLRLTLRRYTSTKKhYNRESKQAPIPSHVFQKLTGG--------NEIAARPIEKILMRL 355
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
56-393 |
2.35e-115 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 341.26 E-value: 2.35e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 56 VHVDLDCFYAQVEMISNPELKDKPLGVQQKY----LVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLTRYREMS 131
Cdd:cd00424 1 LHIDFDNFFASVEQLARPELKGRPVVVVPFNsdstCVIACSYEARKYGVKRGMPVREARKMCPNLILVP-ARLDLYRRLS 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 132 YKVTELLEEFSPVVERLGFDENFVDLTEMVEKrlqqlqsdelsavtvsghvynnqsinlldvlhirLLVGSQIAAEMREA 211
Cdd:cd00424 80 ERLLSELEEVAPLVEVASIDELFLDLTGSARL----------------------------------LGLGSEVALRIKRH 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 212 MYNQLG-LTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIhSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFS 290
Cdd:cd00424 126 IAEQLGgITASIGIASNKLLAKLAAKYAKPDGLTILDPEDLPGFL-SKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAAS 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 291 PKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVE-AKNKIEELLASLLNRVCQDGRKPHTVRL 369
Cdd:cd00424 205 PDALLALWGGVSGERLWYALRGIDDEPLSPPRPRKSFSHERVLPRDSRNAEdARPLLRLLLEKLARRLRRDGRGATRLRL 284
|
330 340
....*....|....*....|....*.
gi 1192727312 370 IIRRYSSE--KHYGRESRQCPIPSHV 393
Cdd:cd00424 285 WLRTVDGRwsGHADIPSRSAPRPIST 310
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
56-426 |
7.80e-83 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 257.45 E-value: 7.80e-83
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 56 VHVDLDCFYAQVEMISNPELKDKPLGV---QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLTRYREMSY 132
Cdd:cd03586 1 IHIDMDAFYASVEQRDNPELKGKPVAVggsSDRGVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPP-RFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 133 KVTELLEEFSPVVERLGFDENFVDltemvekrlqqlqsdelsavtVSGHVYNNQSInlldvlhirllvgSQIAAEMREAM 212
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLD---------------------VTDYVRLFGSA-------------TEIAKEIRARI 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 213 YNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPK 292
Cdd:cd03586 126 REETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPEDVEEFLAPL-PVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVE 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 293 ILEKELGiSVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSF-KKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLII 371
Cdd:cd03586 205 LLKKLFG-KSGRRLYELARGIDNRPVEPDRERKSIGVERTFsEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKL 283
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 1192727312 372 RRYSSEKHygreSRQCPIPSHViqklgTGLQSPDFCASSLMQRRLEDKLVKLEGV 426
Cdd:cd03586 284 KYADFSTR----TRSRTLPEPT-----DDAEDIYELALELLEELLDGRPIRLLGV 329
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
53-373 |
4.26e-81 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 253.14 E-value: 4.26e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 53 RVIVHVDLDCFYAQVEMISNPELKDKPLGV---QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLTRYRE 129
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVggdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPP-DFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 130 MSYKVTELLEEFSPVVERLGFDENFVDLTEMvekrlqqlqsdelsavtvsghvynnqsinlldvlhiRLLVGS--QIAAE 207
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGS------------------------------------ARLFGSaeAIARR 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 208 MREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKCLEALGINSVRDLQ 287
Cdd:COG0389 124 IRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAFLAPL-PVEKLWGVGPKTAEKLARLGIRTIGDLA 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 288 TFSPKILEKELGiSVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKcssEVEAKNKIEELLASLLNRVC----QDGRK 363
Cdd:COG0389 203 ALPRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVERTFGE---DLTDLEELEAALRRLAERLAerlrRQGLG 278
|
330
....*....|
gi 1192727312 364 PHTVRLIIRR 373
Cdd:COG0389 279 ARTVTVKLRT 288
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
61-426 |
2.52e-58 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 194.57 E-value: 2.52e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 61 DCFYAQVEMISNPELKDKPLGV----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLTRYREMSYKVTE 136
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVggspGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPG-RFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 137 LLEEFSPVVERLGFDENFVDLTEmvEKRLQQlqsdelSAVTvsghvynnqsinlldvlhirllvgsqIAAEMREAMYNQL 216
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTD--NKLCIG------SATL--------------------------IAQEIRQDIFEEL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 217 GLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEK 296
Cdd:PRK02406 127 GLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFLATLP-VEKIPGVGKVTAEKLHALGIYTCADLQKYDLAELIR 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 297 ELGiSVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKK-CSSEVEAKNKIEEL---LASLLNRVcQDGRKPHTVRLIIR 372
Cdd:PRK02406 206 HFG-KFGRRLYERARGIDERPVKPDRERKSVGVERTFAEdLYDLEACLAELPRLaekLERRLERA-KPDKRIKTVGVKLK 283
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 1192727312 373 RYSSEKhygresrqcpipsHVIQKLGTGLQSPDFC--ASSLMQRRLEDKLVKLEGV 426
Cdd:PRK02406 284 FADFQQ-------------TTKEHTADPLDKADLIelLAQALLRRLGGRGVRLLGV 326
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
56-397 |
5.26e-56 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 189.06 E-value: 5.26e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 56 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLV------NGED------ 123
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNSIIAVSYAARAFGVTRFMTIDEAKKKCPDLILAhvatykKGEDeadyhe 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 124 ----------LTRYREMSYKVTELLEEFSPVVERLGFDENFVDLtemvekrlqqlqsdelsavtvsghvynnqsinlldv 193
Cdd:cd01702 81 npsparhkvsLDPYRRASRKILNILKRFGDVVEKASIDEAYLDL------------------------------------ 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 194 lhirllvGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAK 273
Cdd:cd01702 125 -------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLP-ITSIRGLGGKLGE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 274 CL-EALGINSVRDLQTF--SPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSF--KKCSSEVEAKNKIEE 348
Cdd:cd01702 197 EIiDLLGLPTEGDVAGFrsSESDLQEHFGEKLGEWLYNLLRGIDHEPVKPRPLPKSMGSSKNFpgKTALSTEDVQHWLLV 276
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 1192727312 349 LLASLLNRVCQD----GRKPHTVRLiirRYSSEKHYGRESRQCPIPSHVIQKL 397
Cdd:cd01702 277 LASELNSRLEDDryenNRRPKTLVL---SLRQRGDGVRRSRSCALPRYDAQKI 326
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
52-426 |
2.59e-55 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 188.31 E-value: 2.59e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 52 SRVIVHVDLDCFYAQVEMISNPELKDKPLGV-----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLTR 126
Cdd:PRK01810 4 GRVIFHVDMNSFFASVEIAYDPSLQGKPLAVagnekERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRR-PNFDR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 127 YREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKrlqqlqsdelsavtvsGHVYnnqsinlldvlhirllvgsQIAA 206
Cdd:PRK01810 83 YREASRQMFQILSEFTPLVQPVSIDEGYLDITDCYAL----------------GSPL-------------------EIAK 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 207 EMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:PRK01810 128 MIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRKRDVPEMLWPL-PVGEMHGIGEKTAEKLKDIGIQTIGDL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 287 QTFSPKILEKELGISvAQRIQKLSFGEDNSPVilsgPPQSFSEEDS------FKKCSSEV-EAKNKIEELLASLLNRVCQ 359
Cdd:PRK01810 207 AKADEHILRAKLGIN-GVRLQRRANGIDDRPV----DPEAIYQFKSvgnsttLSHDMDEEkELLDVLRRLSKSVSKRLQK 281
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 360 DGRKPHTVRLIIrRYSSEKHYGREsrqcpipshviQKLGTGLQSPD---FCASSLMQRRLEDKLVKLEGV 426
Cdd:PRK01810 282 KTVVSYNVQIMI-RYHDRRTITRS-----------KTLKNPIWEKRdifQAASRLFKQHWNGDPVRLLGV 339
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
58-235 |
1.22e-47 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 160.05 E-value: 1.22e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 58 VDLDCFYAQVEMISNPELKDKPLGVQQKY---LVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVnGEDLTRYREMSYKV 134
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNgrgIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-PPDLELYRRASRKI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 135 TELLEEFSPV-VERLGFDENFVDLTEMVEkrlqqlqsdelsavtvsghvynnqsinlldvlhiRLLVGSQIAAEMREAMY 213
Cdd:pfam00817 80 FEILRRFSTPkVEQASIDEAFLDLTGLEK----------------------------------LFGAEEALAKRLRREIA 125
|
170 180
....*....|....*....|..
gi 1192727312 214 NQLGLTGCAGVASNKLLAKLVS 235
Cdd:pfam00817 126 EETGLTCSIGIAPNKLLAKLAS 147
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
23-373 |
1.17e-43 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 157.48 E-value: 1.17e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 23 AWAMELADVGAAASSQ-GVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGV-QQKYL---VVTCNYEARK 97
Cdd:cd01701 16 TWKARLKDFFRELSNGsKEADPSNSIHPDLQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVcHGKGPnseIASCNYEARS 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 98 LGVKKLMNVRDAKEKCPQLVLVnGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEkrlqqlqsdelsavt 177
Cdd:cd01701 96 YGIKNGMWVGQAKKLCPQLVTL-PYDFEAYEEVSLTFYEILASYTDNIEAVSCDEALIDITSLLE--------------- 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 178 vsghvynnQSINLLDVLhirllvGSQIAAEMREAmynqlglTGC---AGVASNKLLAKLVSGVFKPNQQTVLLPESCQHL 254
Cdd:cd01701 160 --------ETYELPEEL------AEAIRNEIRET-------TGCsasVGIGPNILLARLATRKAKPDGQYHLSAEKVEEF 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 255 IHSLNhIKEIPGIGYKTA-KCLEALG-INSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDS 332
Cdd:cd01701 219 LSQLK-VGDLPGVGSSLAeKLVKLFGdTCGGLELRSKTKEKLQKVLGPKTGEKLYDYCRGIDDRPVTGEKERKSVSAEIN 297
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 1192727312 333 ----FKKcssEVEAKNKIEELLASLLNRVCQDGRKPHTVRL-IIRR 373
Cdd:cd01701 298 ygirFTN---VDDVEQFLQRLSEELSKRLEESNVTGRQITLkLMKR 340
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
50-374 |
1.97e-42 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 155.09 E-value: 1.97e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 50 ASSRVIVHVDLDCFYAQVEMISNPELKDKPL---GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTR 126
Cdd:PRK03348 2 RAQRWVLHLDMDAFFASVEQLTRPTLRGRPVlvgGLGGRGVVAGASYEARVFGARSAMPMHQARRLVGNGAVVLPPRFVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 127 YREMSYKVTELLEEFSPVVERLGFDENFVDLTEmvekrlqqLQSDELSAVTvsghvynnqsinlldvlhirllvgsQIAA 206
Cdd:PRK03348 82 YRAASRRVFDTLRELSPVVEQLSFDEAFVEPAE--------LAGASAEEVE-------------------------AFAE 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 207 EMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:PRK03348 129 RLRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIRVVPPGEERELLAPLP-VRRLWGIGPVTEEKLHRLGIETIGDL 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 287 QTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKK-CSSEVEAKNKIEELLASLLNRVCQDGRKPH 365
Cdd:PRK03348 208 AALSEAEVANLLGATVGPALHRLARGIDDRPVAERAEAKQISAESTFAVdLTTRAQLREAIERIAEHAHRRLLKDGRGAR 287
|
....*....
gi 1192727312 366 TVRLIIRRY 374
Cdd:PRK03348 288 TVTVKLRKS 296
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
53-379 |
3.25e-40 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 146.79 E-value: 3.25e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 53 RVIVHVDLDCFYAQVEMISNPELKDKPL---GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLTRYRE 129
Cdd:PRK14133 3 RVIIHVDMDAFFASVEQMDNPKLKGKPVivgGISERGVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVR-HERYKE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 130 MSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKrlqqlqsdelsavtvsghvynnqsinlldvlhirllvGSQIAAEMR 209
Cdd:PRK14133 82 VSKNIFKILYEVTPIVEPVSIDEAYLDITNIKEE-------------------------------------PIKIAKYIK 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 210 EAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDLQTF 289
Cdd:PRK14133 125 KKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKIITEDMIPDILKPLP-ISKVHGIGKKSVEKLNNIGIYTIEDLLKL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 290 SPKILEK---ELGISVAQRIQklsfGEDNSPVILSGPPQSFSEEDSFKKcssEVEAKNKIEELLASLLNRVCQDGRKPH- 365
Cdd:PRK14133 204 SREFLIEyfgKFGVEIYERIR----GIDYREVEVSRERKSIGKETTLKK---DTKDKEELKKYLKDFSNIISEELKKRNl 276
|
330
....*....|....*..
gi 1192727312 366 ---TVRLIIRRYSSEKH 379
Cdd:PRK14133 277 ygkTVTVKIKTSDFQTH 293
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
55-333 |
6.63e-36 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 136.60 E-value: 6.63e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 55 IVHVDLDCFYAQVEMISNPELKDKPL--GVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGeDLTRYREMSY 132
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPViiGGGKRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKP-DMEKYVRVGR 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 133 KVTELLEEFSPVVERLGFDENFVDL--TEmvekRLQqlqsDELSAVTVSghvynnqsinlldvlhiRLlvgsqiAAEMRE 210
Cdd:PRK02794 117 EVRAMMQALTPLVEPLSIDEAFLDLsgTE----RLH----GAPPAVVLA-----------------RF------ARRVER 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 211 amynQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSlNHIKEIPGIGYKTAKCLEALGINSVRDLQTFS 290
Cdd:PRK02794 166 ----EIGITVSVGLSYNKFLAKIASDLDKPRGFSVIGRAEALAFLAP-KPVGIIWGVGPATAARLARDGIRTIGDLQRAD 240
|
250 260 270 280
....*....|....*....|....*....|....*....|...
gi 1192727312 291 PKILEKELGiSVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSF 333
Cdd:PRK02794 241 EADLMRRFG-SMGLRLWRLARGIDDRKVSPDREAKSVSAETTF 282
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
58-397 |
1.12e-35 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 134.21 E-value: 1.12e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 58 VDLDCFYAQVEMISNPELKDKPLGVqqkyL------VVTCNYEARKLGVKKLMNVRDAKEKCPQL-VLVNGEDLTRYREM 130
Cdd:cd01700 3 VDCNSFYASCERVFRPLLLGRPLVV----LsnndgcVIARSPEAKALGIKMGSPYFKVPDLLERHgVAVFSSNYALYGDM 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 131 SYKVTELLEEFSPVVERLGFDENFVDLTemvekrlqqlqsdelsavtvsgHVYNNQSINlldvlhirllvgsQIAAEMRE 210
Cdd:cd01700 79 SRRIMSILERFSPDVEVYSIDESFLDLT----------------------GSLRFGDLE-------------ELARKIRR 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 211 AMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQT----VLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:cd01700 124 RILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYggvvDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 287 QTFSPKILEKELGIsVAQRIQKLSFGEDNSPVILSGPP-QSFSEEDSFKKcssEVEAKNKIEELLASLLNRVC----QDG 361
Cdd:cd01700 204 AQADPDLLRKKFGV-VGERLVRELNGIDCLPLEEYPPPkKSIGSSRSFGR---DVTDLDELKQALAEYAERAAeklrRQK 279
|
330 340 350
....*....|....*....|....*....|....*...
gi 1192727312 362 RKPHTVRLIIR--RYSSEKHYGRESRQCPIPSHVIQKL 397
Cdd:cd01700 280 SVARTISVFIGtsGFSRQPKYYSATNTLPYPTNDTREI 317
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
53-427 |
4.49e-35 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 134.36 E-value: 4.49e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 53 RVIVHVDLDCFYAQVEMISNPELKDKPLGV-----QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLTRY 127
Cdd:PRK03103 3 RVILLVDMQSFYASVEKAANPELKGRPVIVsgdpeRRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPR-MQRY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 128 REMSYKVTELLEEFSPVVERLGFDENFVDLTemvekrlqqlqsdelsavtvsghvynnqsinlldvlHIRLLVGS--QIA 205
Cdd:PRK03103 82 IDVSLQITRILEDFTDLVEPFSIDEQFLDVT------------------------------------GSQKLFGSplEIA 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 206 AEMREAMYNQLGLTGCAGVASNKLLAKLVSGVF---KPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINS 282
Cdd:PRK03103 126 QKIQQRIMRETGVYARVGIGPNKLLAKMACDNFakkNPDGLFTLDKEDVPADLWPLP-VRKLFGVGSRMEKHLRRMGIRT 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 283 VRDLQTFSPKILEKELGIsVAQRIQKLSFGEDNSPVilsgPPQSFSEEDSFKKCSS---EVEAKNKIEELLASLLNRVCQ 359
Cdd:PRK03103 205 IGQLANTPLERLKKRWGI-NGEVLWRTANGIDYSPV----TPHSLDRQKAIGHQMTlprDYRGFEEIKVVLLELCEEVCR 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 360 DGRKPH----TVRLIIRrySSEKHYGRE-SRQCPIPSHV----------------------IQKLG---TGLQSPDFCAS 409
Cdd:PRK03103 280 RARAKGymgrTVSVSLR--GADFDWPTGfSRQMTLPEPTnlamevyeaacklfhrhwdgkpVRRVGvtlSNLVSDDVWQL 357
|
410
....*....|....*...
gi 1192727312 410 SLMQRRleDKLVKLEGVF 427
Cdd:PRK03103 358 SLFGDR--ERKRSLGYVM 373
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
55-318 |
5.08e-35 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 133.96 E-value: 5.08e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 55 IVHVDLDCFYAQVEMISNPELKDKPL----GVqqkylVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEdLTRYREM 130
Cdd:PRK03858 6 ILHADLDSFYASVEQRDDPALRGRPVivggGV-----VLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPR-MSAYSRA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 131 SYKVTELLEEFSPVVERLGFDENFVDltemvekrlqqlqsdelsavtVSGhvynnqsinlldvlhIRLLVG--SQIAAEM 208
Cdd:PRK03858 80 SKAVFEVFRDTTPLVEGLSIDEAFLD---------------------VGG---------------LRRISGtpVQIAARL 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 209 REAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDLQT 288
Cdd:PRK03858 124 RRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRELAFLHPLP-VRRLWGVGPVTAAKLRAHGITTVGDVAE 202
|
250 260 270
....*....|....*....|....*....|
gi 1192727312 289 FSPKILEKELGISVAQRIQKLSFGEDNSPV 318
Cdd:PRK03858 203 LPESALVSLLGPAAGRHLHALAHNRDPRRV 232
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
54-318 |
1.05e-34 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 131.84 E-value: 1.05e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 54 VIVHVDLDCFYAQVEMISNPELKDKPLGV-------QQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTr 126
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVcvysgrfEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKEV- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 127 YREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKrlqqlqsdelsavtvsghvyNNQSINLldvlhirllvgsqiAA 206
Cdd:PRK01216 81 YQQVSNRIMKLLREYSEKIEIASIDEAYLDISDKVKN--------------------YQDAYNL--------------GL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 207 EMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:PRK01216 127 EIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRFINELD-IADIPGIGDITAEKLKKLGVNKLVDT 205
|
250 260 270
....*....|....*....|....*....|..
gi 1192727312 287 QTFSPKILEKELGISVAQRIQKLSFGEDNSPV 318
Cdd:PRK01216 206 LRIEFDELKGIIGEAKAKYLFSLARNEYNEPV 237
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
53-372 |
4.77e-33 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 127.45 E-value: 4.77e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 53 RVIVHVDLDCFYAQVEMISNPELKDKPLGV------QQKYLVVTC-NYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLT 125
Cdd:PRK03352 5 RWVLHVDLDQFIAAVELLRRPELAGLPVIVggngdpTEPRKVVTCaSYEARAFGVRAGMPLRTAARRCPDAVFLP-SDPA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 126 RYREMSYKVTELLEEFSPVVERLGFDENFVdltemvekrlqqlqsdelsAVTVSGHVynnqsinlldvlhirllvgsQIA 205
Cdd:PRK03352 84 AYDAASEEVMATLRDLGVPVEVWGWDEAFL-------------------GVDTDDPE--------------------ALA 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 206 AEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRD 285
Cdd:PRK03352 125 EEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRLTDANWMAVMGDRP-TDALWGVGPKTAKRLAALGITTVAD 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 286 LQTFSPKILEKELGISVAQRIQKLSFGEDNSPVIlSGP--PQSFSEEDSFKK---CSSEVEAknKIEELLASLLNRVCQD 360
Cdd:PRK03352 204 LAAADPAELAATFGPTTGPWLLLLARGGGDTEVS-AEPwvPRSRSREVTFPQdltDRAEVES--AVRELARRVLDEVVAE 280
|
330
....*....|..
gi 1192727312 361 GRKPHTVRLIIR 372
Cdd:PRK03352 281 GRPVTRVAVKVR 292
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
56-379 |
8.92e-21 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 94.70 E-value: 8.92e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 56 VHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLTRYREMSYKVT 135
Cdd:PTZ00205 136 IHLDMDMFYAAVEIKKHPEYAAIPLAIGTMTMLQTANYVARGRGIRQGMPGFLALKICPNLLILP-PDFDAYNEESNTVR 214
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 136 ELLEEFSPVVERLGFDENFVDLTEMVEkRLQQLQSDElsavtvsghvynnqsinlldvlhirllvgsQIAAEMREAMYNQ 215
Cdd:PTZ00205 215 RIVAEYDPNYISFGLDELTLEVSAYIE-RFEGTKTAE------------------------------DVASELRVRVFGE 263
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 216 LGLTGCAGVASNKLLAKLVSGVFKPNQQTVL---LPESCQHLIHSLNhIKEIPGIGYKTAKCLEALGINSVRDLQT---- 288
Cdd:PTZ00205 264 TKLTASAGIGPTAALAKIASNINKPNGQHDLnlhTRGDVMTYVRDLG-LRSVPGVGKVTEALLKGLGITTLSDIYNrrve 342
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 289 ----FSPKILEKELGISVAqrIQKL-----SFGEDNSPVILSGPPQSFSEEDSFKKcsseVEAKNKIEELLASLLNRVCQ 359
Cdd:PTZ00205 343 lcyiLHNNLFRFLLGASIG--IMQWpdaatAANTENCEGATGGQRKAISSERSFTT----PRTKEGLQEMVDTVFNGAYE 416
|
330 340
....*....|....*....|....
gi 1192727312 360 DGRKPH----TVRLIIrRYSSEKH 379
Cdd:PTZ00205 417 EMRKSElmcrQISLTI-RWASYRY 439
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
57-313 |
6.44e-17 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 82.12 E-value: 6.44e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 57 HVDLDCFYAQVEMISNPELKDKPLGV--QQKYLVVTCNYEARKLGVK---KLMNVRDAKEKCPQLVLVNGEDLtrYREMS 131
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVlsNNDGCVIARSAEAKALGIKmgdPWFKQKDLFRRCGVVCFSSNYEL--YADMS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 132 YKVTELLEEFSPVVERLGFDENFVDLTemvekrlqqlqsdelsavtvsghvynnqsinllDVLHIRLLvgSQIAAEMREA 211
Cdd:PRK03609 82 NRVMSTLEELSPRVEIYSIDEAFCDLT---------------------------------GVRNCRDL--TDFGREIRAT 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 212 MYNQLGLTGCAGVASNKLLAKLVSGVFKP-NQQT---VLL--PESCQHLIhSLNHIKEIPGIGYKTAKCLEALGINSVRD 285
Cdd:PRK03609 127 VLQRTHLTVGVGIAQTKTLAKLANHAAKKwQRQTggvVDLsnLERQRKLL-SLQPVEEVWGVGRRISKKLNAMGIKTALD 205
|
250 260
....*....|....*....|....*...
gi 1192727312 286 LQTFSPKILEKELGISVAQRIQKLSfGE 313
Cdd:PRK03609 206 LADTNIRFIRKHFNVVLERTVRELR-GE 232
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
73-328 |
1.23e-07 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 53.15 E-value: 1.23e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 73 PELKDKPLGV--QQKYLVVT-CNYEARKLGVKKLMNVRDAKEKCPQLVLVNgEDLTRYREMSYKVTELLEEFSPVVERLG 149
Cdd:cd03468 18 PADDEAPLAVveRKKAGRILaCNAAARAAGVRPGMPLAEALALCPNLQVVE-YDPEADARALQELALWLLRFTPLVALDG 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 150 FDENFVDltemvekrlqqlqsdelsaVTVSGHVYNNQsINLLDVLHIRLLVgsqiaaemreamynqLGLTGCAGVASNKL 229
Cdd:cd03468 97 PDGLLLD-------------------VTGCLHLFGGE-DALAASLRAALAT---------------LGLSARAGIADTPG 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 230 LAKLVSgvFKPNQQTVLLPESCQHLIHSLNH-IKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQK 308
Cdd:cd03468 142 AAWLLA--RAGGGRGVLRREALAAALVLLAPlPVAALRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQ 219
|
250 260
....*....|....*....|
gi 1192727312 309 LsFGEDNSPVILSGPPQSFS 328
Cdd:cd03468 220 A-YGRDPEPLLFSPPPPAFD 238
|
|
| TIGR03491 |
TIGR03491 |
RecB family nuclease, putative, TM0106 family; Members of this uncharacterized protein family ... |
260-325 |
1.26e-04 |
|
RecB family nuclease, putative, TM0106 family; Members of this uncharacterized protein family are found broadly but sporadically among bacteria. The N-terminal region is homologous to the Cas4 protein of CRISPR systems, although this protein family shows no signs of association with CRISPR repeats.
Pssm-ID: 274603 [Multi-domain] Cd Length: 457 Bit Score: 44.24 E-value: 1.26e-04
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1192727312 260 HIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEK--ELGISVAQRIQK--LSFGEdNSPVILSGPPQ 325
Cdd:TIGR03491 208 HLSLVPGIGPSRYRLLQELGIHTLEDLAAADPNDLEDfgEQGLGVAEQLVQqaRAQLE-GRPILLAPPKA 276
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
248-280 |
1.87e-04 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 38.53 E-value: 1.87e-04
10 20 30
....*....|....*....|....*....|...
gi 1192727312 248 PESCQHLIHSLNhIKEIPGIGYKTAKCLEALGI 280
Cdd:pfam11798 1 PDDVPEFLWPLP-ISKIPGIGKKLAEKLKALGI 32
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
324-393 |
2.86e-04 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 39.85 E-value: 2.86e-04
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1192727312 324 PQSFSEEDSF-KKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYssekHYGRESRQCPIPSHV 393
Cdd:pfam11799 1 RKSIGAERTFgRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKIRYS----DFRTITRSVTLPSPT 67
|
|
| radA |
PRK04301 |
DNA repair and recombination protein RadA; Validated |
261-308 |
2.52e-03 |
|
DNA repair and recombination protein RadA; Validated
Pssm-ID: 235273 [Multi-domain] Cd Length: 317 Bit Score: 39.86 E-value: 2.52e-03
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|
gi 1192727312 261 IKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIS--VAQRIQK 308
Cdd:PRK04301 8 LEDLPGVGPATAEKLREAGYDTVEAIAVASPKELSEAAGIGesTAAKIIE 57
|
|
| HHH_5 |
pfam14520 |
Helix-hairpin-helix domain; |
263-306 |
2.67e-03 |
|
Helix-hairpin-helix domain;
Pssm-ID: 434010 [Multi-domain] Cd Length: 57 Bit Score: 35.93 E-value: 2.67e-03
10 20 30 40
....*....|....*....|....*....|....*....|....*.
gi 1192727312 263 EIPGIGYKTAKCLEALGINSVRDLQTFSPKILEK--ELGISVAQRI 306
Cdd:pfam14520 6 SISGIGPKTALALLSAGIGTVEDLAEADVDELAEipGIGEKTAQRI 51
|
|
| RecG |
COG1200 |
RecG-like helicase [Replication, recombination and repair]; |
261-286 |
5.43e-03 |
|
RecG-like helicase [Replication, recombination and repair];
Pssm-ID: 440813 [Multi-domain] Cd Length: 684 Bit Score: 39.26 E-value: 5.43e-03
10 20
....*....|....*....|....*.
gi 1192727312 261 IKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:COG1200 8 LTYLKGVGPKRAKLLAKLGIRTVGDL 33
|
|
| PRK10917 |
PRK10917 |
ATP-dependent DNA helicase RecG; Provisional |
261-286 |
6.07e-03 |
|
ATP-dependent DNA helicase RecG; Provisional
Pssm-ID: 236794 [Multi-domain] Cd Length: 681 Bit Score: 38.98 E-value: 6.07e-03
10 20
....*....|....*....|....*.
gi 1192727312 261 IKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:PRK10917 11 LTSLKGVGPKTAEKLAKLGIHTVQDL 36
|
|
| Cdd1 |
pfam11731 |
Pathogenicity locus; Cdd1 is expressed as part of the pathogenicity locus operon in several ... |
255-286 |
6.44e-03 |
|
Pathogenicity locus; Cdd1 is expressed as part of the pathogenicity locus operon in several different orders of bacteria. Many members of the family are annotated as being putative mitomycin resistance proteins but this could not be confirmed.
Pssm-ID: 463333 [Multi-domain] Cd Length: 82 Bit Score: 35.66 E-value: 6.44e-03
10 20 30
....*....|....*....|....*....|..
gi 1192727312 255 IHSLNHIKEIPGIGYKTAKCLEALGINSVRDL 286
Cdd:pfam11731 2 RDGLKELQDIPNIGPAIAEDLRLLGIRSPEEL 33
|
|
| |
