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Conserved domains on  [gi|1394533216|ref|NP_001350747|]
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metaxin-3 isoform 3 [Homo sapiens]

Protein Classification

metaxin( domain architecture ID 12107616)

metaxin, a glutathione S-transferase (GST) family protein, is a component of the preprotein import complex of the mitochondrial outer membrane and may function in the transport of proteins into the mitochondrion

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GST_C_Metaxin1_3 cd03212
C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione ...
 105-241 8.42e-78

C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione S-transferase (GST) C-terminal domain family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and similar proteins. Mammalian metaxin (or metaxin 1) is a component of the preprotein import complex of the mitochondrial outer membrane. Metaxin extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. Like the murine gene, the human metaxin gene is located downstream to the glucocerebrosidase (GBA) pseudogene and is convergently transcribed. Inherited deficiency of GBA results in Gaucher disease, which presents many diverse clinical phenotypes. Alterations in the metaxin gene, in addition to GBA mutations, may be associated with Gaucher disease. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken, and mammals.


:

Pssm-ID: 198321 [Multi-domain]  Cd Length: 137  Bit Score: 233.29  E-value: 8.42e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 105 PAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRMSKGALNRILLTRGQPPLYHLREVEAQIYRDAKECLNLLSNRL 184
Cdd:cd03212     1 PALLYTLWVDEKNYVEVTRPWYAKALPFPLNFYYPGRYQRRAKDRLQLLRGLSELDSEEEVEKELYKDAKECLNLLSERL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1394533216 185 GTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDILSSYF 241
Cdd:cd03212    81 GEKKFFFGDRPTSLDALVFGYLAPLLKAPLPNNKLQNHLKGCPNLVQFVDRILQNYF 137
Tom37 pfam10568
Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer ...
 23-142 2.77e-39

Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer membrane proteins that make up the TOM complex for guiding cytosolic mitochondrial beta-barrel proteins from the cytosol across the outer mitochondrial membrane into the intra-membrane space. In conjunction with TOM70 it guides peptides without an MTS into TOM40, the protein that forms the passage through the outer membrane. It has homology with Metaxin-1, also part of the outer mitochondrial membrane beta-barrel protein transport complex.


:

Pssm-ID: 463150  Cd Length: 126  Bit Score: 134.29  E-value: 2.77e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216  23 SLVVMAYAKFSGAPLKVNVI--DNTWRGSRGDVPILTTED-DMVSQPAKILNFLRKQ--KYNADYELSAKQGADTLAYIA 97
Cdd:pfam10568   1 CLAAIAYLKLLALPAPFEVVpsNNPDLSPTGELPALIDGDgTWVSGFDSIVEYLRSKggGYDLDADLSAKQKADSLAYIS 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1394533216  98 LLEEKLLPAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRM 142
Cdd:pfam10568  81 LLESKLYPLLLYSLWVDEENYEEVTRPAYSKLLPFPLNYILPLKY 125
 
Name Accession Description Interval E-value
GST_C_Metaxin1_3 cd03212
C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione ...
 105-241 8.42e-78

C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione S-transferase (GST) C-terminal domain family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and similar proteins. Mammalian metaxin (or metaxin 1) is a component of the preprotein import complex of the mitochondrial outer membrane. Metaxin extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. Like the murine gene, the human metaxin gene is located downstream to the glucocerebrosidase (GBA) pseudogene and is convergently transcribed. Inherited deficiency of GBA results in Gaucher disease, which presents many diverse clinical phenotypes. Alterations in the metaxin gene, in addition to GBA mutations, may be associated with Gaucher disease. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken, and mammals.


Pssm-ID: 198321 [Multi-domain]  Cd Length: 137  Bit Score: 233.29  E-value: 8.42e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 105 PAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRMSKGALNRILLTRGQPPLYHLREVEAQIYRDAKECLNLLSNRL 184
Cdd:cd03212     1 PALLYTLWVDEKNYVEVTRPWYAKALPFPLNFYYPGRYQRRAKDRLQLLRGLSELDSEEEVEKELYKDAKECLNLLSERL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1394533216 185 GTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDILSSYF 241
Cdd:cd03212    81 GEKKFFFGDRPTSLDALVFGYLAPLLKAPLPNNKLQNHLKGCPNLVQFVDRILQNYF 137
Tom37 pfam10568
Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer ...
 23-142 2.77e-39

Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer membrane proteins that make up the TOM complex for guiding cytosolic mitochondrial beta-barrel proteins from the cytosol across the outer mitochondrial membrane into the intra-membrane space. In conjunction with TOM70 it guides peptides without an MTS into TOM40, the protein that forms the passage through the outer membrane. It has homology with Metaxin-1, also part of the outer mitochondrial membrane beta-barrel protein transport complex.


Pssm-ID: 463150  Cd Length: 126  Bit Score: 134.29  E-value: 2.77e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216  23 SLVVMAYAKFSGAPLKVNVI--DNTWRGSRGDVPILTTED-DMVSQPAKILNFLRKQ--KYNADYELSAKQGADTLAYIA 97
Cdd:pfam10568   1 CLAAIAYLKLLALPAPFEVVpsNNPDLSPTGELPALIDGDgTWVSGFDSIVEYLRSKggGYDLDADLSAKQKADSLAYIS 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1394533216  98 LLEEKLLPAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRM 142
Cdd:pfam10568  81 LLESKLYPLLLYSLWVDEENYEEVTRPAYSKLLPFPLNYILPLKY 125
GST_N_Metaxin1_like cd03078
GST_N family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and ...
 5-77 1.35e-30

GST_N family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and similar proteins including Tom37 from fungi. Mammalian metaxin (or metaxin 1) and the fungal protein Tom37 are components of preprotein import complexes of the mitochondrial outer membrane. Metaxin extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. Like the murine gene, the human metaxin gene is located downstream to the glucocerebrosidase (GBA) pseudogene and is convergently transcribed. Inherited deficiency of GBA results in Gaucher disease, which presents many diverse clinical phenotypes. Alterations in the metaxin gene, in addition to GBA mutations, may be associated with Gaucher disease. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken and mammals.


Pssm-ID: 239376  Cd Length: 73  Bit Score: 110.05  E-value: 1.35e-30
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1394533216   5 LELSCWGGGWGLPSVHSESLVVMAYAKFSGAPLKVNVIDNTWRGSRGDVPILTTEDDMVSQPAKILNFLRKQK 77
Cdd:cd03078     1 MELHVWGGDWGLPSVDPECLAVLAYLKFAGAPLKVVPSNNPWRSPTGKLPALLTSGTKISGPEKIIEYLRKQG 73
GST_C_6 pfam17171
Glutathione S-transferase, C-terminal domain; This domain is closely related to PF00043.
 173-236 1.01e-24

Glutathione S-transferase, C-terminal domain; This domain is closely related to PF00043.


Pssm-ID: 465369  Cd Length: 64  Bit Score: 94.52  E-value: 1.01e-24
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394533216 173 AKECLNLLSNRLGTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDI 236
Cdd:pfam17171   1 AKKDLRALSERLGDKPFFFGDKPTSLDALVFGHLALILYTPLPSPALRIHLKEYPNLVAYCERI 64
 
Name Accession Description Interval E-value
GST_C_Metaxin1_3 cd03212
C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione ...
 105-241 8.42e-78

C-terminal, alpha helical domain of Metaxin 1, Metaxin 3, and similar proteins; Glutathione S-transferase (GST) C-terminal domain family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and similar proteins. Mammalian metaxin (or metaxin 1) is a component of the preprotein import complex of the mitochondrial outer membrane. Metaxin extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. Like the murine gene, the human metaxin gene is located downstream to the glucocerebrosidase (GBA) pseudogene and is convergently transcribed. Inherited deficiency of GBA results in Gaucher disease, which presents many diverse clinical phenotypes. Alterations in the metaxin gene, in addition to GBA mutations, may be associated with Gaucher disease. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken, and mammals.


Pssm-ID: 198321 [Multi-domain]  Cd Length: 137  Bit Score: 233.29  E-value: 8.42e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 105 PAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRMSKGALNRILLTRGQPPLYHLREVEAQIYRDAKECLNLLSNRL 184
Cdd:cd03212     1 PALLYTLWVDEKNYVEVTRPWYAKALPFPLNFYYPGRYQRRAKDRLQLLRGLSELDSEEEVEKELYKDAKECLNLLSERL 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1394533216 185 GTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDILSSYF 241
Cdd:cd03212    81 GEKKFFFGDRPTSLDALVFGYLAPLLKAPLPNNKLQNHLKGCPNLVQFVDRILQNYF 137
Tom37 pfam10568
Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer ...
 23-142 2.77e-39

Outer mitochondrial membrane transport complex protein; The TOM37 protein is one of the outer membrane proteins that make up the TOM complex for guiding cytosolic mitochondrial beta-barrel proteins from the cytosol across the outer mitochondrial membrane into the intra-membrane space. In conjunction with TOM70 it guides peptides without an MTS into TOM40, the protein that forms the passage through the outer membrane. It has homology with Metaxin-1, also part of the outer mitochondrial membrane beta-barrel protein transport complex.


Pssm-ID: 463150  Cd Length: 126  Bit Score: 134.29  E-value: 2.77e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216  23 SLVVMAYAKFSGAPLKVNVI--DNTWRGSRGDVPILTTED-DMVSQPAKILNFLRKQ--KYNADYELSAKQGADTLAYIA 97
Cdd:pfam10568   1 CLAAIAYLKLLALPAPFEVVpsNNPDLSPTGELPALIDGDgTWVSGFDSIVEYLRSKggGYDLDADLSAKQKADSLAYIS 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1394533216  98 LLEEKLLPAVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRM 142
Cdd:pfam10568  81 LLESKLYPLLLYSLWVDEENYEEVTRPAYSKLLPFPLNYILPLKY 125
GST_N_Metaxin1_like cd03078
GST_N family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and ...
 5-77 1.35e-30

GST_N family, Metaxin subfamily, Metaxin 1-like proteins; composed of metaxins 1 and 3, and similar proteins including Tom37 from fungi. Mammalian metaxin (or metaxin 1) and the fungal protein Tom37 are components of preprotein import complexes of the mitochondrial outer membrane. Metaxin extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. Like the murine gene, the human metaxin gene is located downstream to the glucocerebrosidase (GBA) pseudogene and is convergently transcribed. Inherited deficiency of GBA results in Gaucher disease, which presents many diverse clinical phenotypes. Alterations in the metaxin gene, in addition to GBA mutations, may be associated with Gaucher disease. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken and mammals.


Pssm-ID: 239376  Cd Length: 73  Bit Score: 110.05  E-value: 1.35e-30
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1394533216   5 LELSCWGGGWGLPSVHSESLVVMAYAKFSGAPLKVNVIDNTWRGSRGDVPILTTEDDMVSQPAKILNFLRKQK 77
Cdd:cd03078     1 MELHVWGGDWGLPSVDPECLAVLAYLKFAGAPLKVVPSNNPWRSPTGKLPALLTSGTKISGPEKIIEYLRKQG 73
GST_C_6 pfam17171
Glutathione S-transferase, C-terminal domain; This domain is closely related to PF00043.
 173-236 1.01e-24

Glutathione S-transferase, C-terminal domain; This domain is closely related to PF00043.


Pssm-ID: 465369  Cd Length: 64  Bit Score: 94.52  E-value: 1.01e-24
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1394533216 173 AKECLNLLSNRLGTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDI 236
Cdd:pfam17171   1 AKKDLRALSERLGDKPFFFGDKPTSLDALVFGHLALILYTPLPSPALRIHLKEYPNLVAYCERI 64
GST_N_Metaxin cd03054
GST_N family, Metaxin subfamily; composed of metaxins and related proteins. Metaxin 1 is a ...
 5-76 8.02e-22

GST_N family, Metaxin subfamily; composed of metaxins and related proteins. Metaxin 1 is a component of a preprotein import complex of the mitochondrial outer membrane. It extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. In humans, alterations in the metaxin gene may be associated with Gaucher disease. Metaxin 2 binds to metaxin 1 and may also play a role in protein translocation into the mitochondria. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken and mammals. Sequence analysis suggests that all three metaxins share a common ancestry and that they possess similarity to GSTs. Also included in the subfamily are uncharacterized proteins with similarity to metaxins, including a novel GST from Rhodococcus with toluene o-monooxygenase and glutamylcysteine synthetase activities.


Pssm-ID: 239352 [Multi-domain]  Cd Length: 72  Bit Score: 86.90  E-value: 8.02e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1394533216   5 LELSCWGGGWGLPSVHSESLVVMAYAKFSGAPLKVNVIDNTWRGSRGDVPILTTEDDMVSQPAKILNFLRKQ 76
Cdd:cd03054     1 LELYQWGRAFGLPSLSPECLKVETYLRMAGIPYEVVFSSNPWRSPTGKLPFLELNGEKIADSEKIIEYLKKK 72
GST_C_Metaxin2 cd03211
C-terminal, alpha helical domain of Metaxin 2; Glutathione S-transferase (GST) C-terminal ...
 106-236 2.92e-21

C-terminal, alpha helical domain of Metaxin 2; Glutathione S-transferase (GST) C-terminal domain family, Metaxin subfamily, Metaxin 2; a metaxin 1 binding protein identified through a yeast two-hybrid system using metaxin 1 as the bait. Metaxin 2 shares sequence similarity with metaxin 1 but does not contain a C-terminal mitochondrial outer membrane signal-anchor domain. It associates with mitochondrial membranes through its interaction with metaxin 1, which is a component of the mitochondrial preprotein import complex of the outer membrane. The biological function of metaxin 2 is unknown. It is likely that it also plays a role in protein translocation into the mitochondria. However, this has not been experimentally validated. In a recent proteomics study, it has been shown that metaxin 2 is overexpressed in response to lipopolysaccharide-induced liver injury.


Pssm-ID: 198320  Cd Length: 126  Bit Score: 87.32  E-value: 2.92e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 106 AVLHTFWVESDNYFTVTKPWFASQIPFPLSLILPGRMSKGALNRILLTRGQpplyhlREVEAQIYRDAKECLNLLSNRLG 185
Cdd:cd03211     1 AELYISWCDEETYNEVTKPRYGSVYPWPLNHILAYRKQREVLRKLKALGWS------DKSLDQVFDEVEKCCQALSEKLG 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1394533216 186 TSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDI 236
Cdd:cd03211    75 TNQYFFGDQPTELDALVFGHLFTILTTPLPNDELAAIVKKYPNLVEFCRRI 125
GST_N_4 pfam17172
Glutathione S-transferase N-terminal domain; This domain is homologous to pfam02798.
 26-117 8.26e-19

Glutathione S-transferase N-terminal domain; This domain is homologous to pfam02798.


Pssm-ID: 465370 [Multi-domain]  Cd Length: 97  Bit Score: 79.54  E-value: 8.26e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216  26 VMAYAKFSGAPLKVNVIDNTWRGSRGDVPILTTEDDMVSQPAKILNFLRKQKYNADYELSAKQGADTLAYIALLEEKLLP 105
Cdd:pfam17172   6 VETYLRMAGIPYEVEPSSNPSASPKGKLPFIELNGDLIADSEFIIEFLKEKGVDLDAGLSPEQKADARALKALVEEHLYW 85

                          90
                  ....*....|..
gi 1394533216 106 AVLHTFWVESDN 117
Cdd:pfam17172  86 ALLYERWLDPEN 97
GST_C_Metaxin cd03193
C-terminal, alpha helical domain of Metaxin and related proteins; Glutathione S-transferase ...
 168-237 7.69e-18

C-terminal, alpha helical domain of Metaxin and related proteins; Glutathione S-transferase (GST) C-terminal domain family, Metaxin subfamily; composed of metaxins and related proteins. Metaxin 1 is a component of a preprotein import complex of the mitochondrial outer membrane. It extends to the cytosol and is anchored to the mitochondrial membrane through its C-terminal domain. In mice, metaxin is required for embryonic development. In humans, alterations in the metaxin gene may be associated with Gaucher disease. Metaxin 2 binds to metaxin 1 and may also play a role in protein translocation into the mitochondria. Genome sequencing shows that a third metaxin gene also exists in zebrafish, Xenopus, chicken, and mammals. Sequence analysis suggests that all three metaxins share a common ancestry and that they possess similarity to GSTs. Also included in the subfamily are uncharacterized proteins with similarity to metaxins, including a novel GST from Rhodococcus with toluene o-monooxygenase and glutamylcysteine synthetase activities. Other members are the cadmium-inducible lysosomal protein CDR-1 and its homologs from C. elegans, and the failed axon connections (fax) protein from Drosophila. CDR-1 is an integral membrane protein that functions to protect against cadmium toxicity and may also have a role in osmoregulation to maintain salt balance in C. elegans. The fax gene of Drosophila was identified as a genetic modifier of Abelson (Abl) tyrosine kinase. The fax protein is localized in cellular membranes and is expressed in embryonic mesoderm and axons of the central nervous system.


Pssm-ID: 198302 [Multi-domain]  Cd Length: 88  Bit Score: 76.90  E-value: 7.69e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 168 QIYRDAKECLNLLSNRLGTSQFFFGDTPSTLDAYVFGFLAPLYKVRFPKVQLQEHLKQLSNLCRFCDDIL 237
Cdd:cd03193    19 EIYELALEDLEALSTLLGDKKFLFGDKPTSVDATVFAHLASILYPPEDSPLLRVLVASSPNLVEYCERIR 88
GST_C_family cd00299
C-terminal, alpha helical domain of the Glutathione S-transferase family; Glutathione ...
 146-218 9.07e-04

C-terminal, alpha helical domain of the Glutathione S-transferase family; Glutathione S-transferase (GST) family, C-terminal alpha helical domain; a large, diverse group of cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress. In addition, GSTs also show GSH peroxidase activity and are involved in the synthesis of prostaglandins and leukotrienes. This family, also referred to as soluble GSTs, is the largest family of GSH transferases and is only distantly related to the mitochondrial GSTs (GSTK). Soluble GSTs bear no structural similarity to microsomal GSTs (MAPEG family) and display additional activities unique to their group, such as catalyzing thiolysis, reduction and isomerization of certain compounds. The GST fold contains an N-terminal thioredoxin-fold domain and a C-terminal alpha helical domain, with an active site located in a cleft between the two domains. GSH binds to the N-terminal domain while the hydrophobic substrate occupies a pocket in the C-terminal domain. Based on sequence similarity, different classes of GSTs have been identified, which display varying tissue distribution, substrate specificities and additional specific activities. In humans, GSTs display polymorphisms which may influence individual susceptibility to diseases such as cancer, arthritis, allergy and sclerosis. Some GST family members with non-GST functions include glutaredoxin 2, the CLIC subfamily of anion channels, prion protein Ure2p, crystallins, metaxins, stringent starvation protein A, and aminoacyl-tRNA synthetases.


Pssm-ID: 198286 [Multi-domain]  Cd Length: 100  Bit Score: 37.86  E-value: 9.07e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1394533216 146 ALNRILLTRGQPPLYHLREVEaQIYRDAKECLNLLSNRLGTSQFFFGDTPSTLDAYVFGFLAPLYKV--------RFPKV 217
Cdd:cd00299    15 PLVRLLYLEKVPLPKDEAAVE-AAREELPALLAALEQLLAGRPYLAGDQFSLADVALAPVLARLEALgpyydlldEYPRL 93


                  .
gi 1394533216 218 Q 218
Cdd:cd00299    94 K 94
GST_C_2 pfam13410
Glutathione S-transferase, C-terminal domain; This domain is closely related to pfam00043.
 167-207 1.62e-03

Glutathione S-transferase, C-terminal domain; This domain is closely related to pfam00043.


Pssm-ID: 433185 [Multi-domain]  Cd Length: 67  Bit Score: 36.53  E-value: 1.62e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 1394533216 167 AQIYRDAKECLNLLSNRLGTSQFFFGDTPSTLDAYVFGFLA 207
Cdd:pfam13410   3 ERAREQLRAALDALEARLADGPGLLGDRPTLADIALAPVLA 43
GST_C_Beta cd03188
C-terminal, alpha helical domain of Class Beta Glutathione S-transferases; Glutathione ...
 164-203 4.46e-03

C-terminal, alpha helical domain of Class Beta Glutathione S-transferases; Glutathione S-transferase (GST) C-terminal domain family, Class Beta subfamily; GSTs are cytosolic dimeric proteins involved in cellular detoxification by catalyzing the conjugation of glutathione (GSH) with a wide range of endogenous and xenobiotic alkylating agents, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. The GST fold contains an N-terminal thioredoxin-fold domain and a C-terminal alpha helical domain, with an active site located in a cleft between the two domains. GSH binds to the N-terminal domain while the hydrophobic substrate occupies a pocket in the C-terminal domain. Unlike mammalian GSTs which detoxify a broad range of compounds, the bacterial class Beta GSTs exhibit GSH conjugating activity with a narrow range of substrates. In addition to GSH conjugation, they are involved in the protection against oxidative stress and are able to bind antibiotics and reduce the antimicrobial activity of beta-lactam drugs, contributing to antibiotic resistance. The structure of the Proteus mirabilis enzyme reveals that the cysteine in the active site forms a covalent bond with GSH. One member of this subfamily is a GST from Burkholderia xenovorans LB400 that is encoded by the bphK gene and is part of the biphenyl catabolic pathway.


Pssm-ID: 198297 [Multi-domain]  Cd Length: 113  Bit Score: 36.46  E-value: 4.46e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 1394533216 164 EVEAQIYRDAKECLNLLSNRLGTSQFFFGDTPSTLDAYVF 203
Cdd:cd03188    38 EVKAAARERLERRLAYLDAQLAGGPYLLGDQFSVADAYLF 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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