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Conserved domains on  [gi|2048774984|ref|NP_001382072|]
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rap guanine nucleotide exchange factor 5 isoform 2 [Rattus norvegicus]

Protein Classification

guanine nucleotide exchange factor( domain architecture ID 10858405)

Ras guanine nucleotide exchange factor activates Ras-like small GTPases by mediating the replacement of GDP with GTP

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
640-879 1.17e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


:

Pssm-ID: 214539  Cd Length: 242  Bit Score: 273.35  E-value: 1.17e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  640 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTV-NLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 718
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPlNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  719 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 797
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  798 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDMSPKEQQELKSYVNHL-YVIDSQQALFELSHR 875
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 2048774984  876 LEPR 879
Cdd:smart00147 238 IEPR 241
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 98-221 2.13e-60

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


:

Pssm-ID: 239884  Cd Length: 125  Bit Score: 200.65  E-value: 2.13e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  98 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 177
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2048774984 178 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 221
Cdd:cd04437    81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 368-499 1.52e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 105.11  E-value: 1.52e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  368 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 446
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 2048774984  447 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 499
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
640-879 1.17e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 273.35  E-value: 1.17e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  640 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTV-NLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 718
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPlNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  719 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 797
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  798 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDMSPKEQQELKSYVNHL-YVIDSQQALFELSHR 875
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 2048774984  876 LEPR 879
Cdd:smart00147 238 IEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 640-875 3.41e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 261.42  E-value: 3.41e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 640 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTV-NLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 718
Cdd:cd00155     1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKNIHLSpNLERFIERFNNLSNWVASEILLCTNPKKRARLLSK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 719 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMK--PPKIPFM 796
Cdd:cd00155    81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGpnPPCVPFL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 797 PLLLKDVTFIHEGNKTFLD-NLVNFEKLHMIADTVRTLRHCRTNQFgsdmSPKEQQELKSYVNHL-YVIDSQQALFELSH 874
Cdd:cd00155   161 GVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSNSY----ELNRDEDILAFLWKLlELILNEDELYELSL 236


                  .
gi 2048774984 875 R 875
Cdd:cd00155   237 E 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 647-824 1.14e-68

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 225.55  E-value: 1.14e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 647 DLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHC 726
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKIAEHC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 727 KAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFMPLLLKDVTFI 806
Cdd:pfam00617  81 RELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTDLTFI 160

                         170
                  ....*....|....*....
gi 2048774984 807 HEGNKTFL-DNLVNFEKLH 824
Cdd:pfam00617 161 EEGNPDFLeGGLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 98-221 2.13e-60

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 200.65  E-value: 2.13e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  98 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 177
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2048774984 178 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 221
Cdd:cd04437    81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 368-499 1.52e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 105.11  E-value: 1.52e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  368 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 446
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 2048774984  447 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 499
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 375-498 1.25e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 82.46  E-value: 1.25e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 375 TPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY-----SAKKYQGEEENSDVPCRKRkVLHL 449
Cdd:cd06224     1 TLEALIEHLTSTFDMPD------PSFVSTFLLTYRSFTTPTELLEKLIERYeiappENLEYNDWDKKKSKPIRLR-VLNV 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2048774984 450 VSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 498
Cdd:cd06224    74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 114-181 2.57e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.38  E-value: 2.57e-14
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2048774984 114 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVFYQF 181
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKDSYYFYRF 71
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 370-457 1.19e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 61.94  E-value: 1.19e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 370 VVVSGTPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY----SAKKYQGEEENSDV--PCRK 443
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLD------DSFLSTFLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKtlPIRI 74

                          90
                  ....*....|....
gi 2048774984 444 RkVLHLVSQWISLY 457
Cdd:pfam00618  75 R-VLSVLRHWVENY 87
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 114-183 1.11e-10

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.45  E-value: 1.11e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2048774984  114 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQH--LYFQDNYVFYQFSS 183
Cdd:smart00049   7 LRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVNGPnkHTFKDSKALYRFTT 77
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
640-879 1.17e-85

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 273.35  E-value: 1.17e-85
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  640 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTV-NLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 718
Cdd:smart00147   1 LLLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSKKSPSPlNLEAFIRRFNEVSNWVATEILKQTTPKDRAELLSK 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  719 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKM-KPPKIPFMP 797
Cdd:smart00147  81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKLPSKYKKLFEELEELLSPERNYKNYREALSSCnLPPCIPFLG 160

                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  798 LLLKDVTFIHEGNKTFL-DNLVNFEKLHMIADTVRTLRHCRTNqfgSDMSPKEQQELKSYVNHL-YVIDSQQALFELSHR 875
Cdd:smart00147 161 VLLKDLTFIDEGNPDFLeNGLVNFEKRRQIAEILREIRQLQSQ---PYNLRPNRSDIQSLLQQLlDHLDEEEELYQLSLK 237


                   ....
gi 2048774984  876 LEPR 879
Cdd:smart00147 238 IEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
 640-875 3.41e-81

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 261.42  E-value: 3.41e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 640 ILGMNTWDLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTV-NLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKK 718
Cdd:cd00155     1 FLSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDKNIHLSpNLERFIERFNNLSNWVASEILLCTNPKKRARLLSK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 719 FIKIAAHCKAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMK--PPKIPFM 796
Cdd:cd00155    81 FIQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVLSSKLKKLFEELEELVDPSRNFKNYRKLLKSVGpnPPCVPFL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 797 PLLLKDVTFIHEGNKTFLD-NLVNFEKLHMIADTVRTLRHCRTNQFgsdmSPKEQQELKSYVNHL-YVIDSQQALFELSH 874
Cdd:cd00155   161 GVYLKDLTFLHEGNPDFLEgNLVNFEKRRKIAEILREIRQLQSNSY----ELNRDEDILAFLWKLlELILNEDELYELSL 236


                  .
gi 2048774984 875 R 875
Cdd:cd00155   237 E 237
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
 647-824 1.14e-68

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 225.55  E-value: 1.14e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 647 DLALELMSFDWSLFNSIHEQELIYFTFSRQGSGEHTVNLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHC 726
Cdd:pfam00617   1 ELARQLTLIEFELFRKIKPRELLGSAWSKKDKKENSPNIEAMIARFNKLSNWVASEILSEEDLKKRAKVIKKFIKIAEHC 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 727 KAQQNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFMPLLLKDVTFI 806
Cdd:pfam00617  81 RELNNFNSLMAILSGLNSSPISRLKKTWELVSKKYKKTLEELEKLMSPSRNFKNYREALSSASPPCIPFLGLYLTDLTFI 160

                         170
                  ....*....|....*....
gi 2048774984 807 HEGNKTFL-DNLVNFEKLH 824
Cdd:pfam00617 161 EEGNPDFLeGGLINFEKRR 179
DEP_Epac cd04437
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange ...
 98-221 2.13e-60

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in Epac-like proteins. Epac (exchange proteins directly activated by cAMP) proteins are GEFs (guanine-nucleotide-exchange factors) for the small GTPases, Rap1 and Rap2. They are directly regulated by cyclic AMP, a second messenger that plays a role in the control of diverse cellular processes, such as cell adhesion and insulin secretion. Epac-like proteins share a common domain architecture, containing RasGEF, DEP and CAP-effector (cAMP binding) domains. The DEP domain is involved in membrane localization.


Pssm-ID: 239884  Cd Length: 125  Bit Score: 200.65  E-value: 2.13e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  98 AGRALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYV 177
Cdd:cd04437     1 AGRALRNAILSDAPHLIRDRKYHLRTYRQCCVGTELVDWLLQQSPCVQSRSQAVGMWQVLLEEGVLLHVDQELHFQDKYQ 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2048774984 178 FYQFSSDECSYLY-CEFEREEEWQKGVKLLLELVHLIPARAGICD 221
Cdd:cd04437    81 FYRFSDDECSPAPlEKREAEEELQEAVTLLSQLGPDALLRMILRK 125
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 368-499 1.52e-26

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 105.11  E-value: 1.52e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  368 RYVVVSGTPEKILEHLLndlhlaEVQHKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQ-GEEENSDVPCRKRKV 446
Cdd:smart00229   2 GGLIKGGTLEALIEHLT------EAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPEsWVEEKVNPRRVKNRV 75

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|...
gi 2048774984  447 LHLVSQWISLYKDWLHEDEHSKMFLKTIYRNVldDVYEYPILEKELKEFQKIL 499
Cdd:smart00229  76 LNILRTWVENYWEDFEDDPKLISFLLEFLELV--DDEKYPGLVTSLLNLLRRL 126
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 375-498 1.25e-18

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 82.46  E-value: 1.25e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 375 TPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY-----SAKKYQGEEENSDVPCRKRkVLHL 449
Cdd:cd06224     1 TLEALIEHLTSTFDMPD------PSFVSTFLLTYRSFTTPTELLEKLIERYeiappENLEYNDWDKKKSKPIRLR-VLNV 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2048774984 450 VSQWISLYKDWLHEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKI 498
Cdd:cd06224    74 LRTWVENYPYDFFDDEELLELLEEFLNRLVQEGALLQELKKLLRKLLKL 122
DEP cd04371
DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first ...
 100-180 8.87e-16

DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first discovered. The function of this domain is still not clear, but it is believed to be important for the membrane association of the signaling proteins in which it is present. New studies show that the DEP domain of Sst2, a yeast RGS protein is necessary and sufficient for receptor interaction.


Pssm-ID: 239836  Cd Length: 81  Bit Score: 72.76  E-value: 8.87e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 100 RALRNIIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVF 178
Cdd:cd04371     1 DLVRIMLDSDSGVPIKDRKYHLKTYPNCFTGSELVDWLLDNLE-AITREEAVELGQALLKHGLIHHVsDDKHTFRDSYAL 79


                  ..
gi 2048774984 179 YQ 180
Cdd:cd04371    80 YR 81
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 114-181 2.57e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.38  E-value: 2.57e-14
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2048774984 114 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNYVFYQF 181
Cdd:pfam00610   4 LKDRRKHLKTYPNCFTGSEAVDWLMDNLE-IITREEAVELGQLLLDQGLIHHVgDKHGLFKDSYYFYRF 71
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 370-457 1.19e-11

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 61.94  E-value: 1.19e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984 370 VVVSGTPEKILEHLLNDLHLAEvqhketETLLDDFLLTYTVFMTTDDLCQALLRHY----SAKKYQGEEENSDV--PCRK 443
Cdd:pfam00618   1 QVKAGTLEKLVEYLTSTRIMLD------DSFLSTFLLTYRSFTTPAELLELLIERYnippPLDLSSDSYWISKKtlPIRI 74

                          90
                  ....*....|....
gi 2048774984 444 RkVLHLVSQWISLY 457
Cdd:pfam00618  75 R-VLSVLRHWVENY 87
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 114-183 1.11e-10

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.45  E-value: 1.11e-10
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2048774984  114 VKDRVNLKGFYRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQH--LYFQDNYVFYQFSS 183
Cdd:smart00049   7 LRDRKYFLKTYPNCFTGSELVDWLMDNLE-IIDREEAVHLGQLLLDEGLIHHVNGPnkHTFKDSKALYRFTT 77
DEP_1_DEP6 cd04442
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins ...
 98-181 2.23e-08

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239889 [Multi-domain]  Cd Length: 82  Bit Score: 51.82  E-value: 2.23e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2048774984  98 AGRALRniIILQAADLVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQcRSMAIGVWQLLLDMGIMSSV-DQHLYFQDNY 176
Cdd:cd04442     1 TGEQLR--LRLHEAKVIKDRRHHLRTYPNCFVGKELIDWLIEHKEASD-RETAIKIMQKLLDHSIIHHVcDEHKEFKDAK 77


                  ....*
gi 2048774984 177 VFYQF 181
Cdd:cd04442    78 LFYRF 82
DEP_2_DEP6 cd04441
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins ...
 124-181 7.63e-06

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239888  Cd Length: 85  Bit Score: 44.73  E-value: 7.63e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2048774984 124 YRRSCVGSELVDWLLEHCPfVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 181
Cdd:cd04441    29 YERTFVGSEFIDWLLQEGE-AESRREAVQLCRRLLEHGIIQHVSNKHHFFDSNLLYQF 85
DEP_GPR155 cd04443
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like ...
 111-181 1.32e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in GPR155-like proteins. GRP155-like proteins, also known as PGR22, contain an N-terminal permease domain, a central transmembrane region and a C-terminal DEP domain. They are orphan receptors of the class B G protein-coupled receptors. Their function is unknown.


Pssm-ID: 239890 [Multi-domain]  Cd Length: 83  Bit Score: 44.24  E-value: 1.32e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2048774984 111 ADLVKDRVNLKGFYRRSCVGSELVDWLLEhCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 181
Cdd:cd04443    14 QDIVKDRRCGLRTYKGVFCGCDLVSWLIE-VGLAQDRGEAVLYGRRLLQGGVLQHITNEHHFRDENLLYRF 83
DEP_DEPDC5-like cd04449
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in ...
 113-181 4.99e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC5-like proteins. DEPDC5, in human also known as KIAA0645, is a DEP domain containing protein of unknown function.


Pssm-ID: 239896  Cd Length: 83  Bit Score: 42.65  E-value: 4.99e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2048774984 113 LVKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQF 181
Cdd:cd04449    15 GIFDRSWHKGLPSNCFIGSEAVSWLINNFEDVDTREEAVELGQELMNEGLIEHVSGRHPFLDGFYFYYI 83
DEP_PIKfyve cd04448
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange ...
 124-180 7.46e-05

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in fungal RhoGEF (GDP/GTP exchange factor) PIKfyve-like proteins. PIKfyve contains N-terminal Fyve finger and DEP domains, a central chaperonin-like domain and a C-terminal PIPK (phosphatidylinositol phosphate kinase) domain. PIKfyve-like proteins are important phosphatidylinositol (3)-monophosphate (PtdIns(3)P)-5-kinases, producing PtdIns(3,5)P2, which plays a major role in multivesicular body (MVB) sorting and control of retrograde traffic from the vacuole back to the endosome and/or Golgi. PIKfyve itself has been shown to be play a role in regulating early-endosome-to-trans-Golgi network (TGN) retrograde trafficking.


Pssm-ID: 239895  Cd Length: 81  Bit Score: 42.04  E-value: 7.46e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2048774984 124 YRRSCVGSELVDWLLEHCPFVQcRSMAIGVWQLLLDMGIMSSVDQHLYFQDNYVFYQ 180
Cdd:cd04448    25 YTNCILGKELVNWLIRQGKAAT-RVQAIAIGQALLDAGWIECVSDDDLFRDEYALYK 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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