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Conserved domains on  [gi|4758150|ref|NP_004393|]
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DNA fragmentation factor subunit beta isoform 2 [Homo sapiens]

Protein Classification

CIDE_N_CAD and DFF40 domain-containing protein( domain architecture ID 10158420)

CIDE_N_CAD and DFF40 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DFF40 pfam09230
DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins ...
 100-323 5.14e-150

DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins induce DNA fragmentation and chromatin condensation during apoptosis.


:

Pssm-ID: 462717  Cd Length: 226  Bit Score: 420.93  E-value: 5.14e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    100 IQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTV- 178
Cdd:pfam09230   1 IEAARQLLSDENAPERQKLLADLLHNLDENIEAETREEDPDWFEGLESRFKTKSEYMRYSCQSRIRGYFYEVKEYLTKCa 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    179 GAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNL 258
Cdd:pfam09230  81 NAKAREEYLKVLESFRELLKGFDYNGSYFDRSEKESVRLCTPEGWFSCQGPFDEESCSSLHSINPYGSRESRILFSTWNL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758150    259 DHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTH-KLNCDPSRIYK 323
Cdd:pfam09230 161 DHRIEKKRTILPALVEAVENQKGRELNWEYFYRLLFTLENLKLVHIVCHKKTAHdGLSCDPCKIYK 226
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 4-80 4.89e-43

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


:

Pssm-ID: 119368  Cd Length: 77  Bit Score: 143.33  E-value: 4.89e-43
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758150    4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535   1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
 
Name Accession Description Interval E-value
DFF40 pfam09230
DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins ...
 100-323 5.14e-150

DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins induce DNA fragmentation and chromatin condensation during apoptosis.


Pssm-ID: 462717  Cd Length: 226  Bit Score: 420.93  E-value: 5.14e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    100 IQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTV- 178
Cdd:pfam09230   1 IEAARQLLSDENAPERQKLLADLLHNLDENIEAETREEDPDWFEGLESRFKTKSEYMRYSCQSRIRGYFYEVKEYLTKCa 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    179 GAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNL 258
Cdd:pfam09230  81 NAKAREEYLKVLESFRELLKGFDYNGSYFDRSEKESVRLCTPEGWFSCQGPFDEESCSSLHSINPYGSRESRILFSTWNL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758150    259 DHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTH-KLNCDPSRIYK 323
Cdd:pfam09230 161 DHRIEKKRTILPALVEAVENQKGRELNWEYFYRLLFTLENLKLVHIVCHKKTAHdGLSCDPCKIYK 226
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 4-80 4.89e-43

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


Pssm-ID: 119368  Cd Length: 77  Bit Score: 143.33  E-value: 4.89e-43
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758150    4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535   1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 6-79 2.55e-24

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 94.11  E-value: 2.55e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 4758150      6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:pfam02017   1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLLPSEPVTLVLEEDGTEVdDEEYFQTLPDNTELMLLEKGEKWT 75
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
6-78 2.99e-21

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 85.84  E-value: 2.99e-21
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 4758150       6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:smart00266   1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDSPVTLVLEEDGTIVdDEEYFQTLPDNTELMALEKGEKW 74
 
Name Accession Description Interval E-value
DFF40 pfam09230
DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins ...
 100-323 5.14e-150

DNA fragmentation factor 40 kDa; Members of this family of eukaryotic apoptotic proteins induce DNA fragmentation and chromatin condensation during apoptosis.


Pssm-ID: 462717  Cd Length: 226  Bit Score: 420.93  E-value: 5.14e-150
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    100 IQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTV- 178
Cdd:pfam09230   1 IEAARQLLSDENAPERQKLLADLLHNLDENIEAETREEDPDWFEGLESRFKTKSEYMRYSCQSRIRGYFYEVKEYLTKCa 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 4758150    179 GAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNL 258
Cdd:pfam09230  81 NAKAREEYLKVLESFRELLKGFDYNGSYFDRSEKESVRLCTPEGWFSCQGPFDEESCSSLHSINPYGSRESRILFSTWNL 160

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758150    259 DHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTH-KLNCDPSRIYK 323
Cdd:pfam09230 161 DHRIEKKRTILPALVEAVENQKGRELNWEYFYRLLFTLENLKLVHIVCHKKTAHdGLSCDPCKIYK 226
CIDE_N_CAD cd06535
CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 4-80 4.89e-43

CIDE_N domain of CAD nuclease. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and the release of active DFF40/CAD nuclease.


Pssm-ID: 119368  Cd Length: 77  Bit Score: 143.33  E-value: 4.89e-43
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758150    4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06535   1 KPKCVKIRSLNSAQKYGVAAKNLKELLRKGCRLLQLPCAGSRLCLYEDGTEVTEEYFPTLPDNTELVLLTPGQSWQG 77
CIDE_N cd01615
CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) ...
 4-80 3.18e-27

CIDE_N domain, found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins, as well as CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD(DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of ICAD/DFF45, and the CAD/DFF40 and CIDE nucleases during apoptosis. The CIDE-N domain is also found in the FSP27/CIDE-C protein.


Pssm-ID: 119367  Cd Length: 78  Bit Score: 101.85  E-value: 3.18e-27
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4758150    4 KPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd01615   1 PLRPFKVCDSDRSRKKGVAASSLEELLSKACEKLKLPSAPVTLVLEEDGTEVdDEEYFQTLPDNTVLMLLEPGQKWTP 78
CIDE-N pfam02017
CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. ...
 6-79 2.55e-24

CIDE-N domain; This domain is found in CAD nuclease, and ICAD, the inhibitor of CAD nuclease. The two proteins interact through this domain.


Pssm-ID: 460413  Cd Length: 75  Bit Score: 94.11  E-value: 2.55e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 4758150      6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:pfam02017   1 KPFKVCNVDRSRKKGVAASSLEELLQKGCEKLLLPSEPVTLVLEEDGTEVdDEEYFQTLPDNTELMLLEKGEKWT 75
CAD smart00266
Domains present in proteins implicated in post-mortem DNA fragmentation;
6-78 2.99e-21

Domains present in proteins implicated in post-mortem DNA fragmentation;


Pssm-ID: 128562  Cd Length: 74  Bit Score: 85.84  E-value: 2.99e-21
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 4758150       6 KSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:smart00266   1 RPFKVRDHDRNVRKGVAASSLEELLSKVCDKLALPDSPVTLVLEEDGTIVdDEEYFQTLPDNTELMALEKGEKW 74
CIDE_N_A cd06539
CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, ...
 14-78 9.17e-07

CIDE_N domain of CIDE-A proteins. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CIDE (cell death-inducing DFF45-like effector) proteins. These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40, ICAD/DFF45, and CIDE nucleases during apoptosis. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, the CIDE proteins were initially characterized as mitochondrial activators of apoptosis. However, strong metabolic phenotypes of mice lacking CIDE-A and CIDE-B indicated that this family may play critical roles in energy balance.


Pssm-ID: 119372  Cd Length: 78  Bit Score: 46.09  E-value: 9.17e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 4758150   14 RSPRKfGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAW 78
Cdd:cd06539  12 RSSRR-GVMASSLQELISKTLDALVITSGLVTLVLEEDGTVVdTEEFFQTLGDNTHFMVLEKGQKW 76
CIDE_N_ICAD cd06536
CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) ...
 6-80 1.53e-06

CIDE_N domain of ICAD. The CIDE_N (cell death-inducing DFF45-like effector, N-terminal) domain is found at the N-terminus of the CAD nuclease (caspase-activated DNase/DNA fragmentation factor, DFF40) and its inhibitor, ICAD (DFF45). These proteins are associated with the chromatin condensation and DNA fragmentation events of apoptosis; the CIDE_N domain is thought to regulate the activity of the CAD/DFF40 and ICAD/DFF45 during apoptosis. In normal cells, DFF exists in the nucleus as a heterodimer composed of CAD/DFF40 as a latent nuclease and its chaperone and inhibitor subunit ICAD/DFF45. Apoptotic activation of caspase-3 results in the cleavage of DFF45/ICAD and release of active DFF40/CAD nuclease.


Pssm-ID: 119369  Cd Length: 80  Bit Score: 45.56  E-value: 1.53e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 4758150    6 KSVKLRALRSPRKFGVAGRSCQEVLRKGC--LRFQLPERGSRLCLYEDGTELT-EDYFPSVPDNAELVLLTLGQAWQG 80
Cdd:cd06536   3 KPCVVCNVSRQKQHGVAASSLEELRIKACesLGFDSSSAPITLVLAEDGTIVEdEDYFLCLPPNTKFVLLAENEKWAP 80
CIDE_N_FSP27 cd06538
CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, ...
 14-79 3.56e-05

CIDE_N domain of FSP27 proteins. The CIDE-N (cell death-inducing DFF45-like effector, N-terminal) domain is found in the FSP27/CIDE-C protein, which has been identified as a n adipocyte lipid droplet protein that negatively regulates lipolysis and promotes triglyceride accumulation. The CIDE protein family includes 3 members: CIDE-A, CIDE-B, and FSP27(CIDE-C). Based on sequence similarity with DFF40 and DFF45, CIDE proteins were initially characterized as mitochondrial activators of apoptosis. The CIDE-N domain of FSP27 is sufficient to increase apoptosis in vitro when overexpressed.


Pssm-ID: 119371  Cd Length: 79  Bit Score: 41.72  E-value: 3.56e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 4758150   14 RSPRKfGVAGRSCQEVLRKGCLRFQLPErGSRLCLYEDGTEL-TEDYFPSVPDNAELVLLTLGQAWQ 79
Cdd:cd06538  12 RSLRK-GIMADSLEDLLNKVLDALLLDC-ISSLVLDEDGTGVdTEEFFQALADNTVFMVLGKGQKWK 76
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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