|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
10-428 |
6.46e-178 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 511.47 E-value: 6.46e-178
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVR-------- 81
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS-----IIAVSYAARAFGVTRFMTIDEAKKKCPDLILAHVAtykkgede 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 82 --------ESRGKANLTKYREASVEVMEIMSRF-AVIERASIDEAYVDLtsavqerlqklqgqpisadllpstyieglpq 152
Cdd:cd01702 76 adyhenpsPARHKVSLDPYRRASRKILNILKRFgDVVEKASIDEAYLDL------------------------------- 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 153 gpttaeetvqkegmrkqglfqwldslqidnltspdlqltvGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKP 232
Cdd:cd01702 125 ----------------------------------------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKP 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 233 NRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQF--TESQLQSHFGEKNGSWLYAMCRGIEHDPV 310
Cdd:cd01702 165 NAQTILRNDAVASFLSSLPITSIRGLGGKLGEEIIDLLGLPTEGDVAGFrsSESDLQEHFGEKLGEWLYNLLRGIDHEPV 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 311 KPRQLPKTIGCSKNFPGKTALATrEQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKrlSSLRRCCALTRY 390
Cdd:cd01702 245 KPRPLPKSMGSSKNFPGKTALST-EDVQHWLLVLASELNSRLEDDRYENNRRPKTLVLSLRQRGDG--VRRSRSCALPRY 321
|
410 420 430
....*....|....*....|....*....|....*...
gi 5729982 391 DAHKMSHDAFTVIKNCNTSGIQTEWSPPLTMLFLCATK 428
Cdd:cd01702 322 DAQKIVKDAFKLIKAINEEGLGLAWNYPLTLLSLSFTK 359
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
7-371 |
1.49e-77 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 251.99 E-value: 1.49e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 7 RVVALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGG-IIAVSYEARAFGVTRSM-WADdAKKLCPDLLLAqvresr 84
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVG---GDNNRGvVAAASYEARAFGVRSGMpLFQ-ARRLCPDLVVL------ 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 85 gKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAvqerlQKLQGQPisadllpstyieglpqgpttaeetvqk 163
Cdd:COG0389 71 -PPDFELYRDVSRRVMAILERYTpLVEPLSIDEAFLDVTGS-----ARLFGSA--------------------------- 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 164 egmrkqglfqwldslqidnltspdlqltvgAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSV 243
Cdd:COG0389 118 ------------------------------EAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEV 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 244 PQLFSQMPIRKIRSLGGKLgASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSK 323
Cdd:COG0389 168 AAFLAPLPVEKLWGVGPKT-AEKLARLGIRTIGDLAALPRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVER 245
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 5729982 324 NFPgkTALATREQVQWWLLQLAQELEERLTKDRndndRVATQLVVSIR 371
Cdd:COG0389 246 TFG--EDLTDLEELEAALRRLAERLAERLRRQG----LGARTVTVKLR 287
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
14-371 |
5.80e-62 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 210.75 E-value: 5.80e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 14 MDCFFVQVEQRQNPHLRNKPCAVvqykswkGG-----GIIAV-SYEARAFGVtRS-MWADDAKKLCPDLLLAQVResrgk 86
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAV-------GGspgrrGVISTcNYEARKFGV-RSaMPTAQALKLCPDLIFVPGR----- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 87 anLTKYREASVEVMEIMSRF-AVIERASIDEAYVDLTSAVQerlqklqgqpisadllpstyieglPQGPTTAeetvqkeg 165
Cdd:PRK02406 68 --FDVYKEVSRQIREIFRRYtDLIEPLSLDEAYLDVTDNKL------------------------CIGSATL-------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 166 mrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQ 245
Cdd:PRK02406 114 ------------------------------IAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDA 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 246 LFSQMPIRKIRslG-GKLGASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKN 324
Cdd:PRK02406 164 FLATLPVEKIP--GvGKVTAEKLHALGIYTCADLQKYDLAELIRHFG-KFGRRLYERARGIDERPVKPDRERKSVGVERT 240
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 5729982 325 FPgkTALATREQVQWWLLQLAQELEERLTkdRNDNDRVATQLVVSIR 371
Cdd:PRK02406 241 FA--EDLYDLEACLAELPRLAEKLERRLE--RAKPDKRIKTVGVKLK 283
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
12-227 |
7.69e-45 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 157.35 E-value: 7.69e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNG--RGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-------PPDLEL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 92 YREASVEVMEIMSRFA--VIERASIDEAYVDLTSavqerLQKLQGQPISadllpstyieglpqgpttaeetvqkegmrkq 169
Cdd:pfam00817 72 YRRASRKIFEILRRFStpKVEQASIDEAFLDLTG-----LEKLFGAEEA------------------------------- 115
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*...
gi 5729982 170 glfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLAC 227
Cdd:pfam00817 116 --------------------------LAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| zf_UBZ |
pfam18439 |
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA ... |
631-661 |
1.60e-11 |
|
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA polymerase eta (EC:2.7.7.7). It is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. The UBZ domain adopts a classical C2H2 zinc-finger structure characterized by a beta-beta-alpha fold.
Pssm-ID: 465769 Cd Length: 32 Bit Score: 59.06 E-value: 1.60e-11
10 20 30
....*....|....*....|....*....|.
gi 5729982 631 DQVPCEKCGSLVPVWDMPEHMDYHFALELQK 661
Cdd:pfam18439 1 DTYVCPRCGKEIPESELPEHEDWHFAKDLQR 31
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
10-428 |
6.46e-178 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 511.47 E-value: 6.46e-178
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVR-------- 81
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNS-----IIAVSYAARAFGVTRFMTIDEAKKKCPDLILAHVAtykkgede 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 82 --------ESRGKANLTKYREASVEVMEIMSRF-AVIERASIDEAYVDLtsavqerlqklqgqpisadllpstyieglpq 152
Cdd:cd01702 76 adyhenpsPARHKVSLDPYRRASRKILNILKRFgDVVEKASIDEAYLDL------------------------------- 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 153 gpttaeetvqkegmrkqglfqwldslqidnltspdlqltvGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKP 232
Cdd:cd01702 125 ----------------------------------------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKP 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 233 NRQTLVSHGSVPQLFSQMPIRKIRSLGGKLGASVIEILGIEYMGELTQF--TESQLQSHFGEKNGSWLYAMCRGIEHDPV 310
Cdd:cd01702 165 NAQTILRNDAVASFLSSLPITSIRGLGGKLGEEIIDLLGLPTEGDVAGFrsSESDLQEHFGEKLGEWLYNLLRGIDHEPV 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 311 KPRQLPKTIGCSKNFPGKTALATrEQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRVQGDKrlSSLRRCCALTRY 390
Cdd:cd01702 245 KPRPLPKSMGSSKNFPGKTALST-EDVQHWLLVLASELNSRLEDDRYENNRRPKTLVLSLRQRGDG--VRRSRSCALPRY 321
|
410 420 430
....*....|....*....|....*....|....*...
gi 5729982 391 DAHKMSHDAFTVIKNCNTSGIQTEWSPPLTMLFLCATK 428
Cdd:cd01702 322 DAQKIVKDAFKLIKAINEEGLGLAWNYPLTLLSLSFTK 359
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
7-371 |
1.49e-77 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 251.99 E-value: 1.49e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 7 RVVALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGG-IIAVSYEARAFGVTRSM-WADdAKKLCPDLLLAqvresr 84
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVG---GDNNRGvVAAASYEARAFGVRSGMpLFQ-ARRLCPDLVVL------ 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 85 gKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAvqerlQKLQGQPisadllpstyieglpqgpttaeetvqk 163
Cdd:COG0389 71 -PPDFELYRDVSRRVMAILERYTpLVEPLSIDEAFLDVTGS-----ARLFGSA--------------------------- 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 164 egmrkqglfqwldslqidnltspdlqltvgAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSV 243
Cdd:COG0389 118 ------------------------------EAIARRIRRRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEV 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 244 PQLFSQMPIRKIRSLGGKLgASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSK 323
Cdd:COG0389 168 AAFLAPLPVEKLWGVGPKT-AEKLARLGIRTIGDLAALPRAELRRRFG-KVGERLYRLARGIDPRPVEPRRPRKSIGVER 245
|
330 340 350 360
....*....|....*....|....*....|....*....|....*...
gi 5729982 324 NFPgkTALATREQVQWWLLQLAQELEERLTKDRndndRVATQLVVSIR 371
Cdd:COG0389 246 TFG--EDLTDLEELEAALRRLAERLAERLRRQG----LGARTVTVKLR 287
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
12-371 |
3.73e-73 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 240.12 E-value: 3.73e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIA-VSYEARAFGVtRS---MWadDAKKLCPDLLLAqvres 83
Cdd:cd03586 3 IDMDAFYASVEQRDNPELKGKPVAV-------GGssdrGVVStASYEARKFGV-RSampIF--QAKKLCPNLIFV----- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 84 rgKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTsavqerlqklqgqpisadllpstyieGLPQGPTTAEEtvq 162
Cdd:cd03586 68 --PPRFDKYREVSRQIMEILREYTpLVEPLSIDEAYLDVT--------------------------DYVRLFGSATE--- 116
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 163 kegmrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGS 242
Cdd:cd03586 117 ---------------------------------IAKEIRARIREETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPED 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 243 VPQLFSQMPIRKIRSLGGKLGASVIEiLGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTIGCS 322
Cdd:cd03586 164 VEEFLAPLPVRKIPGVGKVTAEKLKE-LGIKTIGDLAKLDVELLKKLFG-KSGRRLYELARGIDNRPVEPDRERKSIGVE 241
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 5729982 323 KNFPgkTALATREQVQWWLLQLAQELEERLTKDrndnDRVATQLVVSIR 371
Cdd:cd03586 242 RTFS--EDLTDPEELLEELLELAEELAERLRKR----GLKGRTVTVKLK 284
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
14-371 |
5.80e-62 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 210.75 E-value: 5.80e-62
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 14 MDCFFVQVEQRQNPHLRNKPCAVvqykswkGG-----GIIAV-SYEARAFGVtRS-MWADDAKKLCPDLLLAQVResrgk 86
Cdd:PRK02406 1 MDCFYAAVEMRDNPELRGKPVAV-------GGspgrrGVISTcNYEARKFGV-RSaMPTAQALKLCPDLIFVPGR----- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 87 anLTKYREASVEVMEIMSRF-AVIERASIDEAYVDLTSAVQerlqklqgqpisadllpstyieglPQGPTTAeetvqkeg 165
Cdd:PRK02406 68 --FDVYKEVSRQIREIFRRYtDLIEPLSLDEAYLDVTDNKL------------------------CIGSATL-------- 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 166 mrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQ 245
Cdd:PRK02406 114 ------------------------------IAQEIRQDIFEELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDA 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 246 LFSQMPIRKIRslG-GKLGASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKN 324
Cdd:PRK02406 164 FLATLPVEKIP--GvGKVTAEKLHALGIYTCADLQKYDLAELIRHFG-KFGRRLYERARGIDERPVKPDRERKSVGVERT 240
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 5729982 325 FPgkTALATREQVQWWLLQLAQELEERLTkdRNDNDRVATQLVVSIR 371
Cdd:PRK02406 241 FA--EDLYDLEACLAELPRLAEKLERRLE--RAKPDKRIKTVGVKLK 283
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
12-404 |
2.19e-53 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 187.57 E-value: 2.19e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:cd00424 3 IDFDNFFASVEQLARPELKGRPVVVVPFNS-DSTCVIACSYEARKYGVKRGMPVREARKMCPNLILV-------PARLDL 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 92 YREASVEVMEIMSRFA-VIERASIDEAYVDLTSAVQerlqklqgqpisadllpstyieglpqgpttaeetvqkegmrkqg 170
Cdd:cd00424 75 YRRLSERLLSELEEVApLVEVASIDELFLDLTGSAR-------------------------------------------- 110
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 171 lfqwldslqidnltspdlQLTVGAVIVEEMRAAI-ERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQ 249
Cdd:cd00424 111 ------------------LLGLGSEVALRIKRHIaEQLGGITASIGIASNKLLAKLAAKYAKPDGLTILDPEDLPGFLSK 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 250 MPIRKIRSLGGKLgASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGCSKNFPGKt 329
Cdd:cd00424 173 LPLTDLPGIGAVT-AKRLEAVGINPIGDLLAASPDALLALWGGVSGERLWYALRGIDDEPLSPPRPRKSFSHERVLPRD- 250
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 5729982 330 aLATREQVQWWLLQLAQELEERLTKDrndnDRVATQLVVSIR-VQGDKRLSSLRRCCALTRYdAHKMSHDAFTVIK 404
Cdd:cd00424 251 -SRNAEDARPLLRLLLEKLARRLRRD----GRGATRLRLWLRtVDGRWSGHADIPSRSAPRP-ISTEDGELLHALD 320
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
4-372 |
6.13e-45 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 165.95 E-value: 6.13e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 4 GQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQykswkGGG----IIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQ 79
Cdd:cd01701 44 DLQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVCH-----GKGpnseIASCNYEARSYGIKNGMWVGQAKKLCPQLVTLP 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 80 VresrgkaNLTKYREASVEVMEI-MSRFAVIERASIDEAYVDLTSAVQERlqklqgqpisadllpstyieglpqgPTTAE 158
Cdd:cd01701 119 Y-------DFEAYEEVSLTFYEIlASYTDNIEAVSCDEALIDITSLLEET-------------------------YELPE 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 159 EtvqkegmrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLV 238
Cdd:cd01701 167 E------------------------------------LAEAIRNEIRETTGCSASVGIGPNILLARLATRKAKPDGQYHL 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 239 SHGSVPQLFSQMPIRKIRSLGGKLGASVIEILG-IEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPK 317
Cdd:cd01701 211 SAEKVEEFLSQLKVGDLPGVGSSLAEKLVKLFGdTCGGLELRSKTKEKLQKVLGPKTGEKLYDYCRGIDDRPVTGEKERK 290
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 5729982 318 TIGCSKNFPgkTALATREQVQWWLLQLAQELEERLtkdrNDNDRVATQLVVSIRV 372
Cdd:cd01701 291 SVSAEINYG--IRFTNVDDVEQFLQRLSEELSKRL----EESNVTGRQITLKLMK 339
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
12-227 |
7.69e-45 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 157.35 E-value: 7.69e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNG--RGIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-------PPDLEL 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 92 YREASVEVMEIMSRFA--VIERASIDEAYVDLTSavqerLQKLQGQPISadllpstyieglpqgpttaeetvqkegmrkq 169
Cdd:pfam00817 72 YRRASRKIFEILRRFStpKVEQASIDEAFLDLTG-----LEKLFGAEEA------------------------------- 115
|
170 180 190 200 210
....*....|....*....|....*....|....*....|....*....|....*...
gi 5729982 170 glfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLAC 227
Cdd:pfam00817 116 --------------------------LAKRLRREIAEETGLTCSIGIAPNKLLAKLAS 147
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
5-355 |
2.31e-39 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 151.24 E-value: 2.31e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 5 QDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIA-VSYEARAFGVTRSMWADDAKKLCPdlLLAQ 79
Cdd:PRK03348 3 AQRWVLHLDMDAFFASVEQLTRPTLRGRPVLV-------GGlggrGVVAgASYEARVFGARSAMPMHQARRLVG--NGAV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 80 VRESRGKAnltkYREASVEVMEIMSRFA-VIERASIDEAYVDltsavqerlqklqgqpisadllpstyieglPQG--PTT 156
Cdd:PRK03348 74 VLPPRFVV----YRAASRRVFDTLRELSpVVEQLSFDEAFVE------------------------------PAElaGAS 119
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 157 AEETVQkegmrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQT 236
Cdd:PRK03348 120 AEEVEA---------------------------------FAERLRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIR 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 237 LVSHGSVPQLFSQMPIRKIRSLG----GKLgasviEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKP 312
Cdd:PRK03348 167 VVPPGEERELLAPLPVRRLWGIGpvteEKL-----HRLGIETIGDLAALSEAEVANLLGATVGPALHRLARGIDDRPVAE 241
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 5729982 313 RQLPKTIGCSKNFPgkTALATREQVQWWLLQLAQELEERLTKD 355
Cdd:PRK03348 242 RAEAKQISAESTFA--VDLTTRAQLREAIERIAEHAHRRLLKD 282
|
|
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
11-399 |
3.43e-39 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 149.16 E-value: 3.43e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 11 LVDMDCFFVQVEQRQNPHLRNKPCAVVQyKSwkgggIIA-VSYEARAFGVTRSMWADDAKKLCPDLLLAQvresrGKaNL 89
Cdd:cd01703 2 HLDLDCFYAQVEEIRDPSLKSKPLGIQQ-KY-----IVVtCNYEARRLGVKKLMSIKDAKEICPDLVLVN-----GE-DL 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 90 TKYREASVEVMEIMSRFA---VIERASIDEAYVDLTsavqerlqklqgqpisadllpstyieglpqgpttaeetvqkegm 166
Cdd:cd01703 70 TPFRDMSKKVYRLLRSYSwndRVERLGFDENFMDVT-------------------------------------------- 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 167 rkqglfqwldslqidnltspDLQLTVGAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQT-LVSHGS--V 243
Cdd:cd01703 106 --------------------EMRLLVASHIAYEMRERIENELGLTCCAGIASNKLLAKLVGSVNKPNQQTtLLPPSCadL 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 244 PQLFSQMPIRKIRSLGGKLgASVIEILGIEYMGELTQFTESQLQSH---------------FGEKNGSWLYAMCRGIEHD 308
Cdd:cd01703 166 MDFMDLHDLRKIPGIGYKT-AAKLEAHGISSVRDLQEFSNRNRQTVgaapsllelllmvkeFGEGIGQRIWKLLFGRDTS 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 309 PVKP-RQLPKTIGCSKNFPgKTALATREQVQWWLLQLAQELEERLTKDRNDNDrvatqlVVSIRVQGDKRLSSLRRccAL 387
Cdd:cd01703 245 PVKPaSDFPQQISIEDSYK-KCSLEEIREARNKIEELLASLLERMKQDLQEVK------AGDGRRPHTLRLTLRRY--TS 315
|
410
....*....|..
gi 5729982 388 TRYDAHKMSHDA 399
Cdd:cd01703 316 TKKHYNRESKQA 327
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
4-373 |
3.57e-39 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 149.79 E-value: 3.57e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 4 GQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqvres 83
Cdd:PRK01810 2 KKGRVIFHVDMNSFFASVEIAYDPSLQGKPLAVAGNEKERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVR----- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 84 rgKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTsavqerlqklqgqpisadllpstyieglpqgPTTAEETVq 162
Cdd:PRK01810 77 --RPNFDRYREASRQMFQILSEFTpLVQPVSIDEGYLDIT-------------------------------DCYALGSP- 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 163 kegmrkqglfqwldslqidnltspdlqltvgAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGS 242
Cdd:PRK01810 123 -------------------------------LEIAKMIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRKRD 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 243 VPQLFSQMPIRKIRSLGGKLgASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLP--KTIG 320
Cdd:PRK01810 172 VPEMLWPLPVGEMHGIGEKT-AEKLKDIGIQTIGDLAKADEHILRAKLG-INGVRLQRRANGIDDRPVDPEAIYqfKSVG 249
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 5729982 321 CSKNFPGKTalaTREQVQWWLL-QLAQELEERLTKDRndndrvatqlVVSIRVQ 373
Cdd:PRK01810 250 NSTTLSHDM---DEEKELLDVLrRLSKSVSKRLQKKT----------VVSYNVQ 290
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
6-320 |
6.46e-38 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 145.92 E-value: 6.46e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 6 DRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRg 85
Cdd:PRK03103 2 ERVILLVDMQSFYASVEKAANPELKGRPVIVSGDPERRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPRMQR- 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 86 kanltkYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAvqerlQKLQGQPISadllpstyieglpqgpttaeetvqke 164
Cdd:PRK03103 81 ------YIDVSLQITRILEDFTdLVEPFSIDEQFLDVTGS-----QKLFGSPLE-------------------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 165 gmrkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGL---NKPNRQTLVSHG 241
Cdd:PRK03103 124 -------------------------------IAQKIQQRIMRETGVYARVGIGPNKLLAKMACDNfakKNPDGLFTLDKE 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 242 SVPQLFSQMPIRKIRSLGGKLGASvIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLP--KTI 319
Cdd:PRK03103 173 DVPADLWPLPVRKLFGVGSRMEKH-LRRMGIRTIGQLANTPLERLKKRWG-INGEVLWRTANGIDYSPVTPHSLDrqKAI 250
|
.
gi 5729982 320 G 320
Cdd:PRK03103 251 G 251
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
6-356 |
1.02e-37 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 144.09 E-value: 1.02e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 6 DRVVALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG----GIIAV-SYEARAFGVTRSMWADDAKKLCPDLLLAQV 80
Cdd:PRK14133 2 DRVIIHVDMDAFFASVEQMDNPKLKGKPVIV-------GGiserGVVSTcSYEARKYGVHSAMPVFMAKKRCPHGIFLPV 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 81 RESRgkanltkYREASVEVMEIMSRFA-VIERASIDEAYVDLTSavqerlqklqgqpisadllpstyieglpqgpttaee 159
Cdd:PRK14133 75 RHER-------YKEVSKNIFKILYEVTpIVEPVSIDEAYLDITN------------------------------------ 111
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 160 tVQKEGMRkqglfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVS 239
Cdd:PRK14133 112 -IKEEPIK----------------------------IAKYIKKKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKIIT 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 240 HGSVPQLFSQMPIRKIRSLGGKLGASVIEIlGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPKTI 319
Cdd:PRK14133 163 EDMIPDILKPLPISKVHGIGKKSVEKLNNI-GIYTIEDLLKLSREFLIEYFG-KFGVEIYERIRGIDYREVEVSRERKSI 240
|
330 340 350
....*....|....*....|....*....|....*..
gi 5729982 320 GCSKNFPGKTalATREQVQWWLLQLAQELEERLTKDR 356
Cdd:PRK14133 241 GKETTLKKDT--KDKEELKKYLKDFSNIISEELKKRN 275
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
10-371 |
2.44e-31 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 125.35 E-value: 2.44e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqVRESrgkaNL 89
Cdd:cd01700 1 ALVDCNSFYASCERVFRPLLLGRPLVVL---SNNDGCVIARSPEAKALGIKMGSPYFKVPDLLERHGVA-VFSS----NY 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 90 TKYREASVEVMEIMSRFAV-IERASIDEAYVDLTsavqerlqklqgqpisadllpstyieglpqgpttaeetvqkegmrk 168
Cdd:cd01700 73 ALYGDMSRRIMSILERFSPdVEVYSIDESFLDLT---------------------------------------------- 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 169 qglfqwlDSLQIDNLTSpdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQT-----LVSHGSV 243
Cdd:cd01700 107 -------GSLRFGDLEE----------LARKIRRRILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYggvvdLTDEEVR 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 244 PQLFSQMPIRKI----RSLGGKLGAsvieiLGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGI---EHDPVKPRQlp 316
Cdd:cd01700 170 DKLLKILPVGDVwgigRRTAKKLNA-----MGIHTAGDLAQADPDLLRKKFG-VVGERLVRELNGIdclPLEEYPPPK-- 241
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 5729982 317 KTIGCSKNFPGK-TALATREQVqwwLLQLAQELEERLtkdRNDNdRVATQLVVSIR 371
Cdd:cd01700 242 KSIGSSRSFGRDvTDLDELKQA---LAEYAERAAEKL---RRQK-SVARTISVFIG 290
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
13-371 |
2.36e-28 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 117.78 E-value: 2.36e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 13 DMDCFFVQVEQRQNPHLRNKPCAVvqykswkGGGII-AVSYEARAFGVTRSMWADDAKKLCPDlllAQVRESRGKAnltk 91
Cdd:PRK03858 10 DLDSFYASVEQRDDPALRGRPVIV-------GGGVVlAASYEAKAYGVRTAMGGRQARRLCPQ---AVVVPPRMSA---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 92 YREASVEVMEIMSRFA-VIERASIDEAYVDLTSavqerLQKLQGQPISadllpstyieglpqgpttaeetvqkegmrkqg 170
Cdd:PRK03858 76 YSRASKAVFEVFRDTTpLVEGLSIDEAFLDVGG-----LRRISGTPVQ-------------------------------- 118
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 171 lfqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQM 250
Cdd:PRK03858 119 -------------------------IAARLRRRVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRELAFLHPL 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 251 PIRKIRSLGGKLGASVIEIlGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLPKTIGcsknfpGKTA 330
Cdd:PRK03858 174 PVRRLWGVGPVTAAKLRAH-GITTVGDVAELPESALVSLLGPAAGRHLHALAHNRDPRRVETGRRRRSVG------AQRA 246
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 5729982 331 L----ATREQVQWWLLQLAqeleERLTKDRNDNDRVATQLVVSIR 371
Cdd:PRK03858 247 LgrgpNSPAEVDAVVVALV----DRVARRMRAAGRTGRTVVLRLR 287
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
8-313 |
3.65e-27 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 113.35 E-value: 3.65e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 8 VVALVDMDCFFVQVEQRQNPHLRNKPCAVVQY--KSWKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESrg 85
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVCVYsgRFEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRKE-- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 86 kanltKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAVQErlqklqgqpisadllpstYIEGLPQGPTTAEETVQKE 164
Cdd:PRK01216 80 -----VYQQVSNRIMKLLREYSeKIEIASIDEAYLDISDKVKN------------------YQDAYNLGLEIKNKILEKE 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 165 GMRkqglfqwldslqidnltspdlqLTVgaviveemraaieretgfqcsaGISHNKVLAKLACGLNKPNRQTLVSHGSVP 244
Cdd:PRK01216 137 KIT----------------------VTV----------------------GISKNKVFAKIAADMAKPNGIKVIDDEEVK 172
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 5729982 245 QLFSQMPIRKIRSLgGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPR 313
Cdd:PRK01216 173 RFINELDIADIPGI-GDITAEKLKKLGVNKLVDTLRIEFDELKGIIGEAKAKYLFSLARNEYNEPVRAR 240
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
9-319 |
1.49e-26 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 113.10 E-value: 1.49e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 9 VALVDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGGG----IIAVSYEARAFGVTRSMWADDAKKLCPDlllAQVResr 84
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPVII-------GGGkrgvVSTACYIARIHGVRSAMPMFKALKLCPD---AVVI--- 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 85 gKANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTSAvqERLQklqgqpisadllpstyieglpqgpttaeetvqk 163
Cdd:PRK02794 105 -KPDMEKYVRVGREVRAMMQALTpLVEPLSIDEAFLDLSGT--ERLH--------------------------------- 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 164 eGMrkqglfqwldslqidnltSPdlqltvgAVIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSV 243
Cdd:PRK02794 149 -GA------------------PP-------AVVLARFARRVEREIGITVSVGLSYNKFLAKIASDLDKPRGFSVIGRAEA 202
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 5729982 244 PQLFSQMPIRKIRSLGGKLGASvIEILGIEYMGELTQFTESQLQSHFGEkNGSWLYAMCRGIEHDPVKPRQLPKTI 319
Cdd:PRK02794 203 LAFLAPKPVGIIWGVGPATAAR-LARDGIRTIGDLQRADEADLMRRFGS-MGLRLWRLARGIDDRKVSPDREAKSV 276
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
12-371 |
3.50e-25 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 107.42 E-value: 3.50e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVvqykswkGG--------GIIA-VSYEARAFGVTRSMWADDAKKLCPD-LLLAQVR 81
Cdd:PRK03352 10 VDLDQFIAAVELLRRPELAGLPVIV-------GGngdpteprKVVTcASYEARAFGVRAGMPLRTAARRCPDaVFLPSDP 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 82 ESrgkanltkYREASVEVMEIMSRF-AVIERASIDEAYVDLTSAVQERLqklqgqpisadllpstyieglpqgpttaeet 160
Cdd:PRK03352 83 AA--------YDAASEEVMATLRDLgVPVEVWGWDEAFLGVDTDDPEAL------------------------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 161 vqkegmrkqglfqwldslqidnltspdlqltvgaviVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSH 240
Cdd:PRK03352 124 ------------------------------------AEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRLTD 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 241 GSVPQLFSQMPIRKIRSLGGKLGASVIEiLGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIEHDPV--KPRQlPKT 318
Cdd:PRK03352 168 ANWMAVMGDRPTDALWGVGPKTAKRLAA-LGITTVADLAAADPAELAATFGPTTGPWLLLLARGGGDTEVsaEPWV-PRS 245
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 5729982 319 IGCSKNFPgkTALATREQVQWWLLQLAQeleeRLTKDRNDNDRVATQLVVSIR 371
Cdd:PRK03352 246 RSREVTFP--QDLTDRAEVESAVRELAR----RVLDEVVAEGRPVTRVAVKVR 292
|
|
| zf_UBZ |
pfam18439 |
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA ... |
631-661 |
1.60e-11 |
|
Ubiquitin-Binding Zinc Finger; This is ubiquitin-binding zinc finger (UBZ) domain found in DNA polymerase eta (EC:2.7.7.7). It is important in the recruitment of the polymerase to the stalled replication machinery in translesion synthesis. The UBZ domain adopts a classical C2H2 zinc-finger structure characterized by a beta-beta-alpha fold.
Pssm-ID: 465769 Cd Length: 32 Bit Score: 59.06 E-value: 1.60e-11
10 20 30
....*....|....*....|....*....|.
gi 5729982 631 DQVPCEKCGSLVPVWDMPEHMDYHFALELQK 661
Cdd:pfam18439 1 DTYVCPRCGKEIPESELPEHEDWHFAKDLQR 31
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
18-395 |
6.41e-09 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 58.16 E-value: 6.41e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 18 FVQVEQRQNPHLRNKPCAVVQYKswKGGGIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTkyreasv 97
Cdd:cd03468 9 PLDALLRNRPADDEAPLAVVERK--KAGRILACNAAARAAGVRPGMPLAEALALCPNLQVVEYDPEADARALQ------- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 98 EVMEIMSRF-AVIERASIDEAYVDLTSAVQerlqklqgqpisadllpstyieglpqgpttaeetvqkegmrkqgLFQWLD 176
Cdd:cd03468 80 ELALWLLRFtPLVALDGPDGLLLDVTGCLH--------------------------------------------LFGGED 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 177 SLQIDnltspdlqltvgaviveeMRAAIEReTGFQCSAGISHNKVLAKLACGLNKP---NRQTlvSHGSVPQLFSQMPIR 253
Cdd:cd03468 116 ALAAS------------------LRAALAT-LGLSARAGIADTPGAAWLLARAGGGrgvLRRE--ALAAALVLLAPLPVA 174
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 254 KIRSlgGKLGASVIEILGIEYMGELTQFTESQLQSHFGeKNGSWLYAMCRGIEHDPVKPRQLPktigcsKNFPGKTALAT 333
Cdd:cd03468 175 ALRL--PPETVELLARLGLRTLGDLAALPRAELARRFG-LALLLRLDQAYGRDPEPLLFSPPP------PAFDFRLELQL 245
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 5729982 334 REQVQWWLLQLAQELEERLTKDRNDNDRVATQLVVSIRvQGDKRLSSLRRCCALTRYDAHKM 395
Cdd:cd03468 246 EEPIARGLLFPLRRLLEQLCAFLALRGLGARRLSLTLF-REDGRVTRVLVGLARPSRDDLPL 306
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
12-371 |
2.13e-08 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 57.34 E-value: 2.13e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 12 VDMDCFFVQVEQRQNPHLRNKPCAVVQYKSwkgggIIAVSYEARAFGVTRSMWADDAKKLCPDLLLAqvresrgKANLTK 91
Cdd:PTZ00205 138 LDMDMFYAAVEIKKHPEYAAIPLAIGTMTM-----LQTANYVARGRGIRQGMPGFLALKICPNLLIL-------PPDFDA 205
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 92 YREASVEVMEIMSRFavierasiDEAYVDLTsavqerLQKLQgqpisadLLPSTYIEGLpQGPTTAEEtvqkegmrkqgl 171
Cdd:PTZ00205 206 YNEESNTVRRIVAEY--------DPNYISFG------LDELT-------LEVSAYIERF-EGTKTAED------------ 251
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 172 fqwldslqidnltspdlqltvgavIVEEMRAAIERETGFQCSAGISHNKVLAKLACGLNKPNRQ---TLVSHGSVPQLFS 248
Cdd:PTZ00205 252 ------------------------VASELRVRVFGETKLTASAGIGPTAALAKIASNINKPNGQhdlNLHTRGDVMTYVR 307
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 249 QMPIRKIRSLgGKLGASVIEILGIEYMGELTQfTESQLQSHFGEKNGSWLYAMCRGIEHDPVKPRQLP------------ 316
Cdd:PTZ00205 308 DLGLRSVPGV-GKVTEALLKGLGITTLSDIYN-RRVELCYILHNNLFRFLLGASIGIMQWPDAATAANtencegatggqr 385
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*
gi 5729982 317 KTIGCSKNFpgkTALATREQVQWWLLQLAQELEERLTKdrndNDRVATQLVVSIR 371
Cdd:PTZ00205 386 KAISSERSF---TTPRTKEGLQEMVDTVFNGAYEEMRK----SELMCRQISLTIR 433
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
316-390 |
4.82e-06 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 45.63 E-value: 4.82e-06
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 5729982 316 PKTIGCSKNFPgkTALATREQVQWWLLQLAQELEERLTKDRndndRVATQLVVSIRvqgDKRLSSLRRCCALTRY 390
Cdd:pfam11799 1 RKSIGAERTFG--RDLTDLEELREALLELAEELAERLRRQG----LVARTVTVKIR---YSDFRTITRSVTLPSP 66
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
10-123 |
3.82e-04 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 43.60 E-value: 3.82e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 5729982 10 ALVDMDCFFVQVEQRQNPHLRNKPCAVVqykSWKGGGIIAVSYEARAFGVtrsmwaddaKKLCPDLLLAQVRESRG---- 85
Cdd:PRK03609 3 ALCDVNSFYASCETVFRPDLRGKPVVVL---SNNDGCVIARSAEAKALGI---------KMGDPWFKQKDLFRRCGvvcf 70
|
90 100 110
....*....|....*....|....*....|....*....
gi 5729982 86 KANLTKYREASVEVMEIMSRFA-VIERASIDEAYVDLTS 123
Cdd:PRK03609 71 SSNYELYADMSNRVMSTLEELSpRVEIYSIDEAFCDLTG 109
|
|
| |
