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Conserved domains on  [gi|398364669|ref|NP_012521|]
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Mad3p [Saccharomyces cerevisiae S288C]

Protein Classification

BUB1 family serine/threonine-protein kinase( domain architecture ID 10655001)

BUB1 family serine/threonine-protein kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 57-188 4.55e-51

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


:

Pssm-ID: 214817  Cd Length: 124  Bit Score: 170.10  E-value: 4.55e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398364669    57 NQVKSSFEQRlIDELPALSDPITLYLEYIKWLNNAYPQGGnsKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIEL 136
Cdd:smart00777   1 EQQRQAFEAE-LQDLYEGDDPLDLWLRYIKWTEENYPQGG--KESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEY 77

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 398364669   137 ftrnsFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLG 188
Cdd:smart00777  78 -----CDEPRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
Mad3_BUB1_II pfam08171
Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in ...
 330-397 5.89e-19

Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds BUB3.


:

Pssm-ID: 400476  Cd Length: 65  Bit Score: 80.78  E-value: 5.89e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 398364669  330 KLPIFRDSI---GRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEEskggrlEFSLEEVLAISRNVYKRVRT 397
Cdd:pfam08171   1 KLPIFKDESnnlGRNNPVYKLIEPPGRKPEKIDCNFNLLYPDDDE------EYCIEEILALSRGLYKKQRN 65
 
Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 57-188 4.55e-51

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 214817  Cd Length: 124  Bit Score: 170.10  E-value: 4.55e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398364669    57 NQVKSSFEQRlIDELPALSDPITLYLEYIKWLNNAYPQGGnsKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIEL 136
Cdd:smart00777   1 EQQRQAFEAE-LQDLYEGDDPLDLWLRYIKWTEENYPQGG--KESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEY 77

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 398364669   137 ftrnsFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLG 188
Cdd:smart00777  78 -----CDEPRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
Mad3_BUB1_I pfam08311
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ...
 58-189 4.23e-48

Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 462420  Cd Length: 123  Bit Score: 162.31  E-value: 4.23e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398364669   58 QVKSSFEQRLIDELpaLSDPITLYLEYIKWLNNAYPQGGnsKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELF 137
Cdd:pfam08311   1 QERQQFEEEIREYD--GDDPLEPWLRYIKWTEESYPQGG--KESGLLELLERCTRYFKDDERYKNDPRYLKLWLKYADFC 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 398364669  138 TrnsfmESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLGI 189
Cdd:pfam08311  77 D-----DPRDIFQFLYSNGIGTKLALFYEEWAELLERQGRFKEADEIYQLGI 123
Mad3_BUB1_II pfam08171
Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in ...
 330-397 5.89e-19

Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds BUB3.


Pssm-ID: 400476  Cd Length: 65  Bit Score: 80.78  E-value: 5.89e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 398364669  330 KLPIFRDSI---GRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEEskggrlEFSLEEVLAISRNVYKRVRT 397
Cdd:pfam08171   1 KLPIFKDESnnlGRNNPVYKLIEPPGRKPEKIDCNFNLLYPDDDE------EYCIEEILALSRGLYKKQRN 65
 
Name Accession Description Interval E-value
Mad3_BUB1_I smart00777
Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint ...
 57-188 4.55e-51

Mad3/BUB1 hoMad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 214817  Cd Length: 124  Bit Score: 170.10  E-value: 4.55e-51
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398364669    57 NQVKSSFEQRlIDELPALSDPITLYLEYIKWLNNAYPQGGnsKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIEL 136
Cdd:smart00777   1 EQQRQAFEAE-LQDLYEGDDPLDLWLRYIKWTEENYPQGG--KESGLLTLLERCIRYFEDDERYKNDPRYLKIWLKYAEY 77

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 398364669   137 ftrnsFMESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLG 188
Cdd:smart00777  78 -----CDEPRELFQFLYSKGIGTKLALFYEEWAQLLEAAGRYKKADEVYQLG 124
Mad3_BUB1_I pfam08311
Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved ...
 58-189 4.23e-48

Mad3/BUB1 homology region 1; Proteins containing this domain are checkpoint proteins involved in cell division. This region has been shown to be essential for the binding of the binding of BUB1 and MAD3 to CDC20p.


Pssm-ID: 462420  Cd Length: 123  Bit Score: 162.31  E-value: 4.23e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 398364669   58 QVKSSFEQRLIDELpaLSDPITLYLEYIKWLNNAYPQGGnsKQSGMLTLLERCLSHLKDLERYRNDVRFLKIWFWYIELF 137
Cdd:pfam08311   1 QERQQFEEEIREYD--GDDPLEPWLRYIKWTEESYPQGG--KESGLLELLERCTRYFKDDERYKNDPRYLKLWLKYADFC 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 398364669  138 TrnsfmESRDIFMYMLRNGIGSELASFYEEFTNLLIQKEKFQYAVKILQLGI 189
Cdd:pfam08311  77 D-----DPRDIFQFLYSNGIGTKLALFYEEWAELLERQGRFKEADEIYQLGI 123
Mad3_BUB1_II pfam08171
Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in ...
 330-397 5.89e-19

Mad3/BUB1 homology region 2; This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds BUB3.


Pssm-ID: 400476  Cd Length: 65  Bit Score: 80.78  E-value: 5.89e-19
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 398364669  330 KLPIFRDSI---GRSDPVYQMINTKDQKPEKIDCNFKLIYCEDEEskggrlEFSLEEVLAISRNVYKRVRT 397
Cdd:pfam08171   1 KLPIFKDESnnlGRNNPVYKLIEPPGRKPEKIDCNFNLLYPDDDE------EYCIEEILALSRGLYKKQRN 65
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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