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Conserved domains on  [gi|6324909|ref|NP_014979|]
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Mrs2p [Saccharomyces cerevisiae S288C]

Protein Classification

CorA family magnesium transporter( domain architecture ID 10191269)

CorA family magnesium transporter, belonging to the CorA metal ion transporter (MIT) family, mediates the influx of magnesium ions, similar to mitochondrial inner membrane magnesium transporter MRS2

CATH:  1.20.58.340
Gene Ontology:  GO:0015095|GO:1903830|GO:0016020
PubMed:  17619822|18927203|16720382
SCOP:  4000404|4000464
TCDB:  1.A.35

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 66-374 3.97e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213357  Cd Length: 323  Bit Score: 320.73  E-value: 3.97e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909   66 CTVFNSKGNIISMSEKfpKWSFLTEHSLFPRDLRKIDNSSIDIIPTIMCKPNCIVINLLHIKALIERDKVYVFDTTNPSA 145
Cdd:cd12823   1 WTVIDSTGNATVVELD--KSELLRELGLQPRDLRLLDPTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  146 AaKLSVLMYDLESKLS--------STKNNSQFYEHRALESIFINVMSALETDFKLHSQICIQILNDLENEVNRLKLRHLL 217
Cdd:cd12823  79 A-LVSAFLEELQRRLAssngseseSGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLL 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  218 IKSKDLTLFYQKTLLIRDLLDELLENDDDLANMYLTVKKSPK---------DNFSDLEMLIETYYTQCDEYVQQSESLIQ 288
Cdd:cd12823 158 PLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPerlessrkeDDHEEVEMLLEAYLQQVDELLNKLEELRE 237

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  289 DIKSTEEIVNIILDANRNSLMLLELKVTIYTLGFTVASVLPAFYGMNLKNFIEESEWGFTSVAVFSIVSALYITKKNFNS 368
Cdd:cd12823 238 YIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVTGGSVVGSILIFIVVLRY 317


                ....*.
gi 6324909  369 LRSVTK 374
Cdd:cd12823 318 LRRKRL 323
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 66-374 3.97e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 320.73  E-value: 3.97e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909   66 CTVFNSKGNIISMSEKfpKWSFLTEHSLFPRDLRKIDNSSIDIIPTIMCKPNCIVINLLHIKALIERDKVYVFDTTNPSA 145
Cdd:cd12823   1 WTVIDSTGNATVVELD--KSELLRELGLQPRDLRLLDPTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  146 AaKLSVLMYDLESKLS--------STKNNSQFYEHRALESIFINVMSALETDFKLHSQICIQILNDLENEVNRLKLRHLL 217
Cdd:cd12823  79 A-LVSAFLEELQRRLAssngseseSGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLL 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  218 IKSKDLTLFYQKTLLIRDLLDELLENDDDLANMYLTVKKSPK---------DNFSDLEMLIETYYTQCDEYVQQSESLIQ 288
Cdd:cd12823 158 PLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPerlessrkeDDHEEVEMLLEAYLQQVDELLNKLEELRE 237

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  289 DIKSTEEIVNIILDANRNSLMLLELKVTIYTLGFTVASVLPAFYGMNLKNFIEESEWGFTSVAVFSIVSALYITKKNFNS 368
Cdd:cd12823 238 YIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVTGGSVVGSILIFIVVLRY 317


                ....*.
gi 6324909  369 LRSVTK 374
Cdd:cd12823 318 LRRKRL 323
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
201-359 2.01e-04

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 43.28  E-value: 2.01e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  201 LNDLENEV----NRLKLRHLLIKSKDLTLFyQKTLL-IRDLLDELLENDDDLanmyltVKKSPKDNFSDLEmlietyytq 275
Cdd:COG0598 146 LDELEDELfespDNELLRRLYRLRRELVRL-RRALApLREVLSRLLRGESPL------ISEEDREYLRDVY--------- 209

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  276 cDEYVQqsesLIQDIKSTEEIVNIILDANrNSLMLLELKVTIYTLgfTVASV--LPA-----FYGMNLKNFIE-ESEWGF 347
Cdd:COG0598 210 -DHLER----LLEMVDSLRERLSGLLDAY-LSLISNRQNEIMKVL--TIIAAifLPPtfiagIYGMNFEGMPElDWPYGY 281

                       170
                ....*....|..
gi 6324909  348 TSVAVFSIVSAL 359
Cdd:COG0598 282 PIVLGLMLLIAV 293
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 172-362 3.62e-04

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 42.30  E-value: 3.62e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909    172 HRALESIFINVMSALEtdfKLHSQIcIQILNDLENEVNRLKLRHLLIKSKDLTLFYQKTLLIRDLLDELLENDDDLAnmy 251
Cdd:pfam01544 113 YRLLDEIVDRFLELLE---KLEDEL-DELEDELEDETNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLRREDDPL--- 185

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909    252 ltVKKSPKDNFSDLemLIETyytqcdeyvqqsESLIQDIKSTEEIVNIILDANrNSLMLLELKVTIYTLgfTVASV--LP 329
Cdd:pfam01544 186 --LDDEQKEYLRDL--LDRL------------ERLLEDLDALRERLRSLQDEY-SSLLSERTNRVMKVL--TVVTAifLP 246

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 6324909    330 A-----FYGMNLKNfIEESEWGFTSVAVFSIVSALYIT 362
Cdd:pfam01544 247 LtlitgIYGMNFGG-LPELEWSYGYPFVLILGLMILLA 283
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 66-374 3.97e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 320.73  E-value: 3.97e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909   66 CTVFNSKGNIISMSEKfpKWSFLTEHSLFPRDLRKIDNSSIDIIPTIMCKPNCIVINLLHIKALIERDKVYVFDTTNPSA 145
Cdd:cd12823   1 WTVIDSTGNATVVELD--KSELLRELGLQPRDLRLLDPTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGSSS 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  146 AaKLSVLMYDLESKLS--------STKNNSQFYEHRALESIFINVMSALETDFKLHSQICIQILNDLENEVNRLKLRHLL 217
Cdd:cd12823  79 A-LVSAFLEELQRRLAssngseseSGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDKISTSNLERLL 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  218 IKSKDLTLFYQKTLLIRDLLDELLENDDDLANMYLTVKKSPK---------DNFSDLEMLIETYYTQCDEYVQQSESLIQ 288
Cdd:cd12823 158 PLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPerlessrkeDDHEEVEMLLEAYLQQVDELLNKLEELRE 237

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  289 DIKSTEEIVNIILDANRNSLMLLELKVTIYTLGFTVASVLPAFYGMNLKNFIEESEWGFTSVAVFSIVSALYITKKNFNS 368
Cdd:cd12823 238 YIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMNLPSGLEESPYAFWIVTGGSVVGSILIFIVVLRY 317


                ....*.
gi 6324909  369 LRSVTK 374
Cdd:cd12823 318 LRRKRL 323
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 81-374 4.03e-51

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 174.81  E-value: 4.03e-51
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909   81 KFPKWSFLTEH--SLFPRDLRKIDNSsiDIIPTIMCKP-NCIVINLL---------HIKALIERDKVYVFDTTNPSAaak 148
Cdd:cd12821   8 DDEIRRLLEELesSHLPNDLRDILLG--VSFPRFEEVSgSGIYIQLRkpqkdadtaSFHFLITRDRLITVRLDELPA--- 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  149 LSVLMYDLESKLsstkNNSQFYEHRALESIFINVMSALETDFKLHSQICIQILNDLENEVNRLKLRHLLIKSKDLTLFYQ 228
Cdd:cd12821  83 LDELRQDLRSGQ----MKSAKSAAELLFVLLGAIIKALLTGIDQFEEKLEELEWDLLEGNNAIKLDRILELRRELLRLTN 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  229 KTLLIRDLLDELLENDDDLanmyltvkkspkdNFSDLEMLIETYYTQCDEYVQQSESLIQDIKSTEEIVNIILDANRNSL 308
Cdd:cd12821 159 LIEPQQEVLMALQEAFAEL-------------LFSEDEEELRRTLDRIERLLQLIEEYEQELDTLQDIEEVVSSHRGNEI 225

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 6324909  309 MLLELKVTIYTLGFTVASvlpAFYGMNLKN-FIEESEWGFTSVAVFSIVsalyITKKNFNSLRSVTK 374
Cdd:cd12821 226 MKTLTVVTTVFLPLTLIG---GLWGMNFKNmPELEWKYGYLFVLGLIIL----STAGLYLYLRRKGW 285
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
201-359 2.01e-04

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 43.28  E-value: 2.01e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  201 LNDLENEV----NRLKLRHLLIKSKDLTLFyQKTLL-IRDLLDELLENDDDLanmyltVKKSPKDNFSDLEmlietyytq 275
Cdd:COG0598 146 LDELEDELfespDNELLRRLYRLRRELVRL-RRALApLREVLSRLLRGESPL------ISEEDREYLRDVY--------- 209

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  276 cDEYVQqsesLIQDIKSTEEIVNIILDANrNSLMLLELKVTIYTLgfTVASV--LPA-----FYGMNLKNFIE-ESEWGF 347
Cdd:COG0598 210 -DHLER----LLEMVDSLRERLSGLLDAY-LSLISNRQNEIMKVL--TIIAAifLPPtfiagIYGMNFEGMPElDWPYGY 281

                       170
                ....*....|..
gi 6324909  348 TSVAVFSIVSAL 359
Cdd:COG0598 282 PIVLGLMLLIAV 293
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 259-366 3.06e-04

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 42.49  E-value: 3.06e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  259 KDNFSDLEML----IETyyTQCDEYVQQSESLIQdikSTEEIVNIILDANRNSLMllelKV-TIYTLGFTVASVLPAFYG 333
Cdd:cd12827 181 KLDEEDKELLedviIEN--KQAIEMAEIYSDILS---GLMDAFASVISNNLNNVM----KFlTSITIVLTIPTIITGFYG 251

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 6324909  334 MNLKNFIEESEWGFTSV----AVFSIVSALYITKKNF 366
Cdd:cd12827 252 MNVPLPFADSPYAFWIIllisLLLSVIVALILRRKKY 288
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 172-362 3.62e-04

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 42.30  E-value: 3.62e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909    172 HRALESIFINVMSALEtdfKLHSQIcIQILNDLENEVNRLKLRHLLIKSKDLTLFYQKTLLIRDLLDELLENDDDLAnmy 251
Cdd:pfam01544 113 YRLLDEIVDRFLELLE---KLEDEL-DELEDELEDETNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLRREDDPL--- 185

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909    252 ltVKKSPKDNFSDLemLIETyytqcdeyvqqsESLIQDIKSTEEIVNIILDANrNSLMLLELKVTIYTLgfTVASV--LP 329
Cdd:pfam01544 186 --LDDEQKEYLRDL--LDRL------------ERLLEDLDALRERLRSLQDEY-SSLLSERTNRVMKVL--TVVTAifLP 246

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 6324909    330 A-----FYGMNLKNfIEESEWGFTSVAVFSIVSALYIT 362
Cdd:pfam01544 247 LtlitgIYGMNFGG-LPELEWSYGYPFVLILGLMILLA 283
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 200-365 2.52e-03

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 39.86  E-value: 2.52e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  200 ILNDLENEVNRLKLRhLLIKSKDLTL----FYQKTLL--------IRDLLDELLENDDDLanmyltVKKSPKDNFSDLEM 267
Cdd:cd12822 122 ILDELEDRLDELEDE-ILEEPRREVLrelfALRRELLrlrrilspQREVLNRLLRRENEL------ISEELRPYFRDVYD 194

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6324909  268 LIETYYTQCDEYVQQSESLIQdiksteeiVNIILDANR-NSLMllelKV-TIYTLGFTVASVLPAFYGMNLKNFIE-ESE 344
Cdd:cd12822 195 HLERLAELLDSYRDLLSSLLD--------AYLSLQSNRtNEVM----KVlTIITTIFLPLTFITGIYGMNFEYMPElHWP 262

                       170       180
                ....*....|....*....|....
gi 6324909  345 WGFTSVAVFSIVSA---LYITKKN 365
Cdd:cd12822 263 YGYFIVLGIMLLIAgglLWWFRRK 286
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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