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Conserved domains on  [gi|23111030|ref|NP_037478|]
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sorting nexin-12 isoform 1 [Homo sapiens]

Protein Classification

PX domain-containing protein( domain architecture ID 10163635)

PX (Phox Homology) domain-containing protein may bind phosphoinositides and may function in targeting proteins to membranes

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PX_SNX12 cd07294
The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a ...
27-158 2.38e-92

The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. The specific function of SNX12 has yet to be elucidated.


:

Pssm-ID: 132827  Cd Length: 132  Bit Score: 264.21  E-value: 2.38e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  27 SNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 106
Cdd:cd07294   1 SNFLEIDIFNPQTVGVGRNRFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 80
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 23111030 107 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPG 158
Cdd:cd07294  81 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQDETIDRNYVPG 132
 
Name Accession Description Interval E-value
PX_SNX12 cd07294
The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a ...
27-158 2.38e-92

The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. The specific function of SNX12 has yet to be elucidated.


Pssm-ID: 132827  Cd Length: 132  Bit Score: 264.21  E-value: 2.38e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  27 SNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 106
Cdd:cd07294   1 SNFLEIDIFNPQTVGVGRNRFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 80
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 23111030 107 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPG 158
Cdd:cd07294  81 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQDETIDRNYVPG 132
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
58-149 5.21e-25

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 91.53  E-value: 5.21e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030    58 LPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGKALKRQlpfrgdegiFEESFIEERRQGLEQFINKIAGHPLA 136
Cdd:pfam00787   1 LPTFSLEEWSVRRRYSDFVELHKKLLRKFPsVIIPPLPPKRWLGR---------YNEEFIEKRRKGLEQYLQRLLQHPEL 71
                          90
                  ....*....|...
gi 23111030   137 QNERCLHMFLQEE 149
Cdd:pfam00787  72 RNSEVLLEFLESD 84
COG5391 COG5391
Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function ...
10-138 5.23e-24

Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function prediction only];


Pssm-ID: 227680 [Multi-domain]  Cd Length: 524  Bit Score: 96.79  E-value: 5.23e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  10 RRLNSKPQdLTDAYGPPSNFLEIDIFNPQTVGVG---RARFTTYEVRMRTNLPIFKLKESC---VRRRYSDFEWLKNELE 83
Cdd:COG5391 112 LPTSLQPP-LSTSHTILDYFISSTVSNPQSLTLLvdsRDKHTSYEIITVTNLPSFQLRESRplvVRRRYSDFESLHSILI 190
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 23111030  84 RDS-KIVVPPLPGKALKRQLPFRGdegiFEESFIEERRQGLEQFINKIAGHPLAQN 138
Cdd:COG5391 191 KLLpLCAIPPLPSKKSNSEYYGDR----FSDEFIEERRQSLQNFLRRVSTHPLLSN 242
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
34-147 2.62e-22

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 85.47  E-value: 2.62e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030     34 IFNPQTVGVGRARFTTYEVRMRTNLpifklKESCVRRRYSDFEWLKNELERD-SKIVVPPLPGKALKRQLPfrgdegIFE 112
Cdd:smart00312   1 VVEPEKIGDGKHYYYVIEIETKTGL-----EEWTVSRRYSDFLELHSKLKKHfPRSILPPLPGKKLFGRLN------NFS 69
                           90       100       110
                   ....*....|....*....|....*....|....*.
gi 23111030    113 ESFIEERRQGLEQFINKIAGHP-LAQNERCLHMFLQ 147
Cdd:smart00312  70 EEFIEKRRRGLEKYLQSLLNHPeLINHSEVVLEFLE 105
 
Name Accession Description Interval E-value
PX_SNX12 cd07294
The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a ...
27-158 2.38e-92

The phosphoinositide binding Phox Homology domain of Sorting Nexin 12; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. The specific function of SNX12 has yet to be elucidated.


Pssm-ID: 132827  Cd Length: 132  Bit Score: 264.21  E-value: 2.38e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  27 SNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 106
Cdd:cd07294   1 SNFLEIDIFNPQTVGVGRNRFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRG 80
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 23111030 107 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPG 158
Cdd:cd07294  81 DEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQDETIDRNYVPG 132
PX_SNX3_like cd06894
The phosphoinositide binding Phox Homology domain of Sorting Nexin 3 and related proteins; The ...
29-151 5.52e-85

The phosphoinositide binding Phox Homology domain of Sorting Nexin 3 and related proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily is composed of SNX3, SNX12, and fungal Grd19. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. SNX3/Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.


Pssm-ID: 132804  Cd Length: 123  Bit Score: 245.06  E-value: 5.52e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  29 FLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDE 108
Cdd:cd06894   1 FLEIDVVNPQTHGVGKKRFTDYEVRMRTNLPVFKKKESSVRRRYSDFEWLRSELERDSKIVVPPLPGKALKRQLPFRGDD 80
                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 23111030 109 GIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAI 151
Cdd:cd06894  81 GIFEEEFIEERRKGLETFINKVAGHPLAQNEKCLHMFLQEETI 123
PX_SNX3 cd07293
The phosphoinositide binding Phox Homology domain of Sorting Nexin 3; The PX domain is a ...
29-151 2.68e-81

The phosphoinositide binding Phox Homology domain of Sorting Nexin 3; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX3 associates with early endosomes through a PX domain-mediated interaction with phosphatidylinositol-3-phosphate (PI3P). It associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer. SNX3 is required for the formation of multivesicular bodies, which function as transport intermediates to late endosomes. It also promotes cell surface expression of the amiloride-sensitive epithelial Na+ channel (ENaC), which is critical in sodium homeostasis and maintenance of extracellular fluid volume.


Pssm-ID: 132826  Cd Length: 123  Bit Score: 235.66  E-value: 2.68e-81
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  29 FLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDE 108
Cdd:cd07293   1 FLEIDVTNPQTVGVGRGRFTTYEIRLKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKALFRQLPFRGDD 80
                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 23111030 109 GIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAI 151
Cdd:cd07293  81 GIFDDSFIEERKQGLEQFLNKVAGHPLAQNERCLHMFLQDEII 123
PX_Grd19 cd07295
The phosphoinositide binding Phox Homology domain of fungal Grd19; The PX domain is a ...
29-149 3.76e-36

The phosphoinositide binding Phox Homology domain of fungal Grd19; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Grd19 is involved in the localization of late Golgi membrane proteins in yeast. Grp19 associates with the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, and functions as a cargo-specific adaptor for the retromer.


Pssm-ID: 132828  Cd Length: 116  Bit Score: 121.07  E-value: 3.76e-36
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  29 FLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDS-KIVVPPLPGKALKRQlpfrgd 107
Cdd:cd07295   1 FLEIEVRNPKTHGIGRGMFTDYEIVCRTNIPAFKLRVSSVRRRYSDFEYFRDILERESpRVMIPPLPGKIFTNR------ 74
                        90       100       110       120
                ....*....|....*....|....*....|....*....|...
gi 23111030 108 egiFEESFIEERRQGLEQFINKIAGHPLAQN-ERCLHMFLQEE 149
Cdd:cd07295  75 ---FSDEVIEERRQGLETFLQSVAGHPLLQTgSKVLAAFLQDP 114
PX_SNX1_2_like cd06859
The phosphoinositide binding Phox Homology domain of Sorting Nexins 1 and 2; The PX domain is ...
30-149 4.16e-32

The phosphoinositide binding Phox Homology domain of Sorting Nexins 1 and 2; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX1, SNX2, and similar proteins. They harbor a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. Both domains have been shown to determine the specific membrane-targeting of SNX1. SNX1 and SNX2 are components of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures effcient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex.


Pssm-ID: 132769 [Multi-domain]  Cd Length: 114  Bit Score: 110.75  E-value: 4.16e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGK-ALKRqlpFRgd 107
Cdd:cd06859   1 FEISVTDPVKVGDGMSAYVVYRVTTKTNLPDFKKSEFSVLRRYSDFLWLYERLVEKYPgRIVPPPPEKqAVGR---FK-- 75
                        90       100       110       120
                ....*....|....*....|....*....|....*....|..
gi 23111030 108 egiFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd06859  76 ---VKFEFIEKRRAALERFLRRIAAHPVLRKDPDFRLFLESD 114
PX_SNX10 cd06898
The phosphoinositide binding Phox Homology domain of Sorting Nexin 10; The PX domain is a ...
36-147 2.48e-29

The phosphoinositide binding Phox Homology domain of Sorting Nexin 10; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX10 may be involved in the regulation of endosome homeostasis. Its expression induces the formation of giant vacuoles in mammalian cells.


Pssm-ID: 132808  Cd Length: 113  Bit Score: 103.56  E-value: 2.48e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  36 NPQTVGVGR-ARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIV-VPPLPGKalkrQLPFRGDegifEE 113
Cdd:cd06898   6 DPRTHKEDDwGSYTDYEIFLHTNSMCFTLKTSCVRRRYSEFVWLRNRLQKNALLIqLPSLPPK----NLFGRFN----NE 77
                        90       100       110
                ....*....|....*....|....*....|....
gi 23111030 114 SFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd06898  78 GFIEERQQGLQDFLEKVLQTPLLLSDSRLHLFLQ 111
PX_Vps5p cd06861
The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps5p; The PX domain ...
30-149 2.01e-26

The phosphoinositide binding Phox Homology domain of yeast sorting nexin Vps5p; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Vsp5p is the yeast counterpart of human SNX1 and is part of the retromer complex, which functions in the endosome-to-Golgi retrieval of vacuolar protein sorting receptor Vps10p, the Golgi-resident membrane protein A-ALP, and endopeptidase Kex2. The PX domain of Vps5p binds phosphatidylinositol-3-phosphate (PI3P). Similar to SNX1, Vps5p contains a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. Both domains have been shown to determine the specific membrane-targeting of SNX1.


Pssm-ID: 132771  Cd Length: 112  Bit Score: 96.27  E-value: 2.01e-26
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGKALKrqlpfrgde 108
Cdd:cd06861   1 FEITVGDPHKVGDLTSAHTVYTVRTRTTSPNFEVSSFSVLRRYRDFRWLYRQLQNNHPgVIVPPPPEKQSV--------- 71
                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 23111030 109 GIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd06861  72 GRFDDNFVEQRRAALEKMLRKIANHPVLQKDPDFRLFLESE 112
PX pfam00787
PX domain; PX domains bind to phosphoinositides.
58-149 5.21e-25

PX domain; PX domains bind to phosphoinositides.


Pssm-ID: 459940  Cd Length: 84  Bit Score: 91.53  E-value: 5.21e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030    58 LPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGKALKRQlpfrgdegiFEESFIEERRQGLEQFINKIAGHPLA 136
Cdd:pfam00787   1 LPTFSLEEWSVRRRYSDFVELHKKLLRKFPsVIIPPLPPKRWLGR---------YNEEFIEKRRKGLEQYLQRLLQHPEL 71
                          90
                  ....*....|...
gi 23111030   137 QNERCLHMFLQEE 149
Cdd:pfam00787  72 RNSEVLLEFLESD 84
PX_Atg24p cd06863
The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation ...
47-146 2.35e-24

The phosphoinositide binding Phox Homology domain of yeast Atg24p, an autophagic degradation protein; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. The yeast Atg24p is a sorting nexin (SNX) which is involved in membrane fusion events at the vacuolar surface during pexophagy. This is facilitated via binding of Atg24p to phosphatidylinositol 3-phosphate (PI3P) through its PX domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132773  Cd Length: 118  Bit Score: 91.20  E-value: 2.35e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERD-SKIVVPPLPGKalKRQLPFRGDEgiFEESFIEERRQGLEQ 125
Cdd:cd06863  19 YISYLITTKTNLPSFSRKEFKVRRRYSDFVFLHECLSNDfPACVVPPLPDK--HRLEYITGDR--FSPEFITRRAQSLQR 94
                        90       100
                ....*....|....*....|.
gi 23111030 126 FINKIAGHPLAQNERCLHMFL 146
Cdd:cd06863  95 FLRRISLHPVLSQSKILHQFL 115
COG5391 COG5391
Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function ...
10-138 5.23e-24

Phox homology (PX) domain protein [Intracellular trafficking and secretion / General function prediction only];


Pssm-ID: 227680 [Multi-domain]  Cd Length: 524  Bit Score: 96.79  E-value: 5.23e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  10 RRLNSKPQdLTDAYGPPSNFLEIDIFNPQTVGVG---RARFTTYEVRMRTNLPIFKLKESC---VRRRYSDFEWLKNELE 83
Cdd:COG5391 112 LPTSLQPP-LSTSHTILDYFISSTVSNPQSLTLLvdsRDKHTSYEIITVTNLPSFQLRESRplvVRRRYSDFESLHSILI 190
                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 23111030  84 RDS-KIVVPPLPGKALKRQLPFRGdegiFEESFIEERRQGLEQFINKIAGHPLAQN 138
Cdd:COG5391 191 KLLpLCAIPPLPSKKSNSEYYGDR----FSDEFIEERRQSLQNFLRRVSTHPLLSN 242
PX smart00312
PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function ...
34-147 2.62e-22

PhoX homologous domain, present in p47phox and p40phox; Eukaryotic domain of unknown function present in phox proteins, PLD isoforms, a PI3K isoform.


Pssm-ID: 214610  Cd Length: 105  Bit Score: 85.47  E-value: 2.62e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030     34 IFNPQTVGVGRARFTTYEVRMRTNLpifklKESCVRRRYSDFEWLKNELERD-SKIVVPPLPGKALKRQLPfrgdegIFE 112
Cdd:smart00312   1 VVEPEKIGDGKHYYYVIEIETKTGL-----EEWTVSRRYSDFLELHSKLKKHfPRSILPPLPGKKLFGRLN------NFS 69
                           90       100       110
                   ....*....|....*....|....*....|....*.
gi 23111030    113 ESFIEERRQGLEQFINKIAGHP-LAQNERCLHMFLQ 147
Cdd:smart00312  70 EEFIEKRRRGLEKYLQSLLNHPeLINHSEVVLEFLE 105
PX_SNX7_30_like cd06860
The phosphoinositide binding Phox Homology domain of Sorting Nexins 7 and 30; The PX domain is ...
30-146 5.04e-21

The phosphoinositide binding Phox Homology domain of Sorting Nexins 7 and 30; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. This subfamily consists of SNX7, SNX30, and similar proteins. They harbor a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-6, SNX8, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of the sorting nexins in this subfamily has yet to be elucidated.


Pssm-ID: 132770  Cd Length: 116  Bit Score: 82.38  E-value: 5.04e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGK-ALKRQLPFRGD 107
Cdd:cd06860   1 LFITVDNPEKHVTTLETYITYRVTTKTTRSEFDSSEYSVRRRYQDFLWLRQKLEESHPtHIIPPLPEKhSVKGLLDRFSP 80
                        90       100       110
                ....*....|....*....|....*....|....*....
gi 23111030 108 EgifeesFIEERRQGLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd06860  81 E------FVATRMRALHKFLNRIVEHPVLSFNEHLKVFL 113
PX_SNX1 cd07281
The phosphoinositide binding Phox Homology domain of Sorting Nexin 1; The PX domain is a ...
30-149 2.20e-19

The phosphoinositide binding Phox Homology domain of Sorting Nexin 1; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX1 is both membrane associated and a cytosolic protein that exists as a tetramer in protein complexes. It can associate reversibly with membranes of the endosomal compartment, thereby coating these vesicles. SNX1 is a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures efficient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex. SNX1 contains a Bin/Amphiphysin/Rvs (BAR) domain C-terminal to the PX domain. The PX domain of SNX1 specifically binds phosphatidylinositol-3-phosphate (PI3P) and PI(3,5)P2, while the BAR domain detects membrane curvature. Both domains help determine the specific membrane-targeting of SNX1, which is localized to a microdomain in early endosomes where it regulates cation-independent mannose-6-phosphate receptor retrieval to the trans Golgi network.


Pssm-ID: 132814  Cd Length: 124  Bit Score: 78.56  E-value: 2.20e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNEL-ERDSK--IVVPPLPGKALKRQLPFR- 105
Cdd:cd07281   1 LKVSITDPEKIGDGMNAYVVYKVTTQTSLLMFRSKHFTVKRRFSDFLGLYEKLsEKHSQngFIVPPPPEKSLIGMTKVKv 80
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....
gi 23111030 106 GDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd07281  81 GKEDSSSAEFLERRRAALERYLQRIVSHPSLLQDPDVREFLEKE 124
PX_domain cd06093
The Phox Homology domain, a phosphoinositide binding module; The PX domain is a ...
31-147 2.61e-18

The Phox Homology domain, a phosphoinositide binding module; The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to membranes. Proteins containing PX domains interact with PIs and have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. Many members of this superfamily bind phosphatidylinositol-3-phosphate (PI3P) but in some cases, other PIs such as PI4P or PI(3,4)P2, among others, are the preferred substrates. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction, as in the cases of p40phox, p47phox, and some sorting nexins (SNXs). The PX domain is conserved from yeast to humans and is found in more than 100 proteins. The majority of PX domain-containing proteins are SNXs, which play important roles in endosomal sorting.


Pssm-ID: 132768 [Multi-domain]  Cd Length: 106  Bit Score: 75.09  E-value: 2.61e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  31 EIDIFNPQTVGVGRARFTTYEVRMRTNlpifKLKESCVRRRYSDFEWLKNELERD-SKIVVPPLPGKALKrqlpfrgdeG 109
Cdd:cd06093   1 SVSIPDYEKVKDGGKKYVVYIIEVTTQ----GGEEWTVYRRYSDFEELHEKLKKKfPGVILPPLPPKKLF---------G 67
                        90       100       110
                ....*....|....*....|....*....|....*...
gi 23111030 110 IFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd06093  68 NLDPEFIEERRKQLEQYLQSLLNHPELRNSEELKEFLE 105
PX_SNX2 cd07282
The phosphoinositide binding Phox Homology domain of Sorting Nexin 2; The PX domain is a ...
30-147 2.99e-18

The phosphoinositide binding Phox Homology domain of Sorting Nexin 2; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX2 is a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi. The retromer consists of a cargo-recognition subcomplex and a subcomplex formed by a dimer of sorting nexins (SNX1 and/or SNX2), which ensures efficient cargo sorting by facilitating proper membrane localization of the cargo-recognition subcomplex. Similar to SNX1, SNX2 contains a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX domain of SNX2 preferentially binds phosphatidylinositol-3-phosphate (PI3P), but not PI(3,4,5)P3. Studies on mice deficient with SNX1 and/or SNX2 suggest that they provide an essential function in embryogenesis and are functionally redundant.


Pssm-ID: 132815  Cd Length: 124  Bit Score: 75.86  E-value: 2.99e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELErdSK-----IVVPPLPGKALKRQLPF 104
Cdd:cd07282   1 IEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSRSEFSVRRRFSDFLGLHSKLA--SKylhvgYIVPPAPEKSIVGMTKV 78
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....
gi 23111030 105 R-GDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd07282  79 KvGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLE 122
PX_SNX7 cd07284
The phosphoinositide binding Phox Homology domain of Sorting Nexin 7; The PX domain is a ...
29-146 1.36e-17

The phosphoinositide binding Phox Homology domain of Sorting Nexin 7; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX7 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-6, SNX8, SNX30, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of SNX7 has yet to be elucidated.


Pssm-ID: 132817  Cd Length: 116  Bit Score: 73.86  E-value: 1.36e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  29 FLEIDifNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELER-DSKIVVPPLPGKALKRQLPFRgd 107
Cdd:cd07284   2 FITVD--EPESHVTAIETFITYRVMTKTSRSEFDSSEFEVRRRYQDFLWLKGRLEEaHPTLIIPPLPEKFVMKGMVER-- 77
                        90       100       110
                ....*....|....*....|....*....|....*....
gi 23111030 108 egiFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd07284  78 ---FNEDFIETRRKALHKFLNRIADHPTLTFNEDFKIFL 113
PX_SNX4 cd06864
The phosphoinositide binding Phox Homology domain of Sorting Nexin 4; The PX domain is a ...
47-149 3.71e-17

The phosphoinositide binding Phox Homology domain of Sorting Nexin 4; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX4 is involved in recycling traffic from the sorting endosome (post-Golgi endosome) back to the late Golgi. It shows a similar domain architecture as SNX1-2, among others, containing a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. SNX4 is implicated in the regulation of plasma membrane receptor trafficking and interacts with receptors for EGF, insulin, platelet-derived growth factor and the long form of the leptin receptor.


Pssm-ID: 132774  Cd Length: 129  Bit Score: 72.79  E-value: 3.71e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMR---TNLPIFKLKESC-VRRRYSDFEWLKNELErDS--KIVVPPLPGKalkrQLPFRGDEGI---FEESFIE 117
Cdd:cd06864  23 YTVYLIETKiveHESEEGLSKKLSsLWRRYSEFELLRNYLV-VTypYVIVPPLPEK----RAMFMWQKLSsdtFDPDFVE 97
                        90       100       110
                ....*....|....*....|....*....|..
gi 23111030 118 ERRQGLEQFINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd06864  98 RRRAGLENFLLRVAGHPELCQDKIFLEFLTHE 129
PX_SNX_like cd06865
The phosphoinositide binding Phox Homology domain of SNX-like proteins; The PX domain is a ...
37-149 2.41e-16

The phosphoinositide binding Phox Homology domain of SNX-like proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. This subfamily is composed of uncharacterized proteins, predominantly from plants, with similarity to sorting nexins. A few members show a similar domain architecture as a subfamily of sorting nexins, containing a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain. The PX-BAR structural unit is known to determine specific membrane localization.


Pssm-ID: 132775  Cd Length: 120  Bit Score: 70.53  E-value: 2.41e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  37 PQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSK-IVVPPLPGKALKRQLPFRGDEgifeesF 115
Cdd:cd06865  13 PSRVPLGGPPYISYKVTTRTNIPSYTHGEFTVRRRFRDVVALADRLAEAYRgAFVPPRPDKSVVESQVMQSAE------F 86
                        90       100       110
                ....*....|....*....|....*....|....
gi 23111030 116 IEERRQGLEQFINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd06865  87 IEQRRVALEKYLNRLAAHPVIGLSDELRVFLTLQ 120
PX_SNX30 cd07283
The phosphoinositide binding Phox Homology domain of Sorting Nexin 30; The PX domain is a ...
47-146 1.39e-15

The phosphoinositide binding Phox Homology domain of Sorting Nexin 30; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX30 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-8, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of SNX30 has yet to be elucidated.


Pssm-ID: 132816  Cd Length: 116  Bit Score: 68.57  E-value: 1.39e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKI-VVPPLPGKALKRQLPFRgdegiFEESFIEERRQGLEQ 125
Cdd:cd07283  18 YITYRVTTKTTRTEFDLPEYSVRRRYQDFDWLRNKLEESQPThLIPPLPEKFVVKGVVDR-----FSEEFVETRRKALDK 92
                        90       100
                ....*....|....*....|.
gi 23111030 126 FINKIAGHPLAQNERCLHMFL 146
Cdd:cd07283  93 FLKRIADHPVLSFNEHFNVFL 113
PX_SNX9_18_like cd06862
The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18; The PX domain is ...
68-147 2.88e-15

The phosphoinositide binding Phox Homology domain of Sorting Nexins 9 and 18; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX9, SNX18, and similar proteins. They contain an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain. SNX9 is localized to plasma membrane endocytic sites and acts primarily in clathrin-mediated endocytosis, while SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.


Pssm-ID: 132772  Cd Length: 125  Bit Score: 67.73  E-value: 2.88e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  68 VRRRYSDFEWLKNEL-ERDSKIVVPPLPGKALkrqlpfrgdEGIFEESFIEERRQGLEQFINKIAGHP-LAQNERCLHmF 145
Cdd:cd06862  34 VSRRYKHFDWLYERLvEKYSCIAIPPLPEKQV---------TGRFEEDFIEKRRERLELWMNRLARHPvLSQSEVFRH-F 103

                ..
gi 23111030 146 LQ 147
Cdd:cd06862 104 LT 105
PX_SNX8_Mvp1p_like cd06866
The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p; The PX ...
62-146 2.13e-13

The phosphoinositide binding Phox Homology domain of Sorting Nexin 8 and yeast Mvp1p; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX8 and the yeast counterpart Mvp1p are involved in sorting and delivery of late-Golgi proteins, such as carboxypeptidase Y, to vacuoles.


Pssm-ID: 132776  Cd Length: 105  Bit Score: 62.63  E-value: 2.13e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  62 KLKESCVRRRYSDFEWLKNEL-ERDSKIVVPPLPgkaLKRQLPFRGDEgifeesFIEERRQGLEQFINKIAGHPLAQNER 140
Cdd:cd06866  26 KRFKSTVYRRYSDFVWLHEYLlKRYPYRMVPALP---PKRIGGSADRE------FLEARRRGLSRFLNLVARHPVLSEDE 96

                ....*.
gi 23111030 141 CLHMFL 146
Cdd:cd06866  97 LVRTFL 102
PX_YPT35 cd07280
The phosphoinositide binding Phox Homology domain of the fungal protein YPT35; The PX domain ...
45-146 1.56e-12

The phosphoinositide binding Phox Homology domain of the fungal protein YPT35; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of YPT35 proteins from the fungal subkingdom Dikarya. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of YPT35 binds to phosphatidylinositol 3-phosphate (PI3P). It also serves as a protein interaction domain, binding to members of the Yip1p protein family, which localize to the ER and Golgi. YPT35 is mainly associated with endosomes and together with Yip1p proteins, may be involved in a specific function in the endocytic pathway.


Pssm-ID: 132813  Cd Length: 120  Bit Score: 60.80  E-value: 1.56e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  45 ARFTTYEVRMRTN----LPIFklkescVRRRYSDF----EWLKNELERDSKIVVPPLPGKAlkrqlPFRGDEGIFEESFI 116
Cdd:cd07280  20 GAYVVWKITIETKdligSSIV------AYKRYSEFvqlrEALLDEFPRHKRNEIPQLPPKV-----PWYDSRVNLNKAWL 88
                        90       100       110
                ....*....|....*....|....*....|
gi 23111030 117 EERRQGLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd07280  89 EKRRRGLQYFLNCVLLNPVFGGSPVVKEFL 118
PX_SNARE cd06897
The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi; The PX domain ...
39-147 1.82e-12

The phosphoinositide binding Phox Homology domain of SNARE proteins from fungi; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. This subfamily is composed of fungal proteins similar to Saccharomyces cerevisiae Vam7p. They contain an N-terminal PX domain and a C-terminal SNARE domain. The SNARE (Soluble NSF attachment protein receptor) family of proteins are integral membrane proteins that serve as key factors for vesicular trafficking. Vam7p is anchored at the vacuolar membrane through the specific interaction of its PX domain with phosphatidylinositol-3-phosphate (PI3P) present in bilayers. It plays an essential role in vacuole fusion. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction.


Pssm-ID: 132807  Cd Length: 108  Bit Score: 59.98  E-value: 1.82e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  39 TVGVGRARFTTYEVRMRtnlpiFKLKESCVRRRYSDFEWLKNELERDSKIVVP-PLPGKalkrqlpFRGDEGIFEESFIE 117
Cdd:cd06897   7 TTSVSPKPYTVYNIQVR-----LPLRSYTVSRRYSEFVALHKQLESEVGIEPPyPLPPK-------SWFLSTSSNPKLVE 74
                        90       100       110
                ....*....|....*....|....*....|..
gi 23111030 118 ERRQGLEQFINKIAGHPLA--QNERCLHMFLQ 147
Cdd:cd06897  75 ERRVGLEAFLRALLNDEDSrwRNSPAVKEFLN 106
PX_SNX16 cd07276
The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a ...
44-146 1.24e-10

The phosphoinositide binding Phox Homology domain of Sorting Nexin 16; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX16 contains a central PX domain followed by a coiled-coil region. SNX16 is localized in early and recycling endosomes through the binding of its PX domain to phosphatidylinositol-3-phosphate (PI3P). It plays a role in epidermal growth factor (EGF) signaling by regulating EGF receptor membrane trafficking.


Pssm-ID: 132809  Cd Length: 110  Bit Score: 55.49  E-value: 1.24e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  44 RARFTTYEVRMRtnlpifKLKESC--VRRRYSDFEWLKNELERDSKIVVPPLPGKAlkrqlpFRGDEgiFEESFIEERRQ 121
Cdd:cd07276  17 RARFTVYKIRVE------NKVGDSwfVFRRYTDFVRLNDKLKQMFPGFRLSLPPKR------WFKDN--FDPDFLEERQL 82
                        90       100
                ....*....|....*....|....*
gi 23111030 122 GLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd07276  83 GLQAFVNNIMAHKDIAKCKLVREFF 107
PX_SNX18 cd07286
The phosphoinositide binding Phox Homology domain of Sorting Nexin 18; The PX domain is a ...
68-159 4.21e-09

The phosphoinositide binding Phox Homology domain of Sorting Nexin 18; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX18, like SNX9, contains an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature. The PX-BAR structural unit helps determine specific membrane localization. SNX18 is localized to peripheral endosomal structures, and acts in a trafficking pathway that is clathrin-independent but relies on AP-1 and PACS1.


Pssm-ID: 132819  Cd Length: 127  Bit Score: 51.98  E-value: 4.21e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  68 VRRRYSDFEWLKNEL-ERDSKIVVPPLPGKALKrqlpfrgdeGIFEESFIEERRQGLEQFINKIAGHP-LAQNERCLHMF 145
Cdd:cd07286  34 VHRRYKHFDWLYARLaEKFPVISVPHIPEKQAT---------GRFEEDFISKRRKGLIWWMDHMCSHPvLARCDAFQHFL 104
                        90
                ....*....|....
gi 23111030 146 LQEEAIDRNYVPGK 159
Cdd:cd07286 105 TCPSTDEKAWKQGK 118
PX_SNX41_42 cd06867
The phosphoinositide binding Phox Homology domain of fungal Sorting Nexins 41 and 42; The PX ...
62-149 5.53e-09

The phosphoinositide binding Phox Homology domain of fungal Sorting Nexins 41 and 42; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX41 and SNX42 (also called Atg20p) form dimers with SNX4, and are required in protein recycling from the sorting endosome (post-Golgi endosome) back to the late Golgi in yeast.


Pssm-ID: 132777  Cd Length: 112  Bit Score: 51.10  E-value: 5.53e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  62 KLKESCVRRRYSDFEWLKNELER-DSKIVVPPLPGKA-----LKRQLPFRGDEGIfeesfIEERRQGLEQFINKIAGHPL 135
Cdd:cd06867  24 RLGGSEVKRRYSEFESLRKNLTRlYPTLIIPPIPEKHslkdyAKKPSKAKNDAKI-----IERRKRMLQRFLNRCLQHPI 98
                        90
                ....*....|....
gi 23111030 136 AQNERCLHMFLQEE 149
Cdd:cd06867  99 LRNDIVFQKFLDPN 112
PX_SNX5 cd07291
The phosphoinositide binding Phox Homology domain of Sorting Nexin 5; The PX domain is a ...
30-147 6.15e-09

The phosphoinositide binding Phox Homology domain of Sorting Nexin 5; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX5, abundantly expressed in macrophages, regulates macropinocytosis, a process that enables cells to internalize large amounts of external solutes. It may also be a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, acting as a mammalian equivalent of yeast Vsp17p. It also binds the Fanconi anaemia complementation group A protein (FANCA). SNX5 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to other sorting nexins including SNX1-2. The PX-BAR structural unit helps determine the specific membrane-targeting of some SNXs. The PX domain of SNX5 binds phosphatidylinositol-3-phosphate (PI3P) and PI(3,4)P2. SNX5 is localized to a subdomain of early endosome and is recruited to the plasma membrane following EGF stimulation and elevation of PI(3,4)P2 levels.


Pssm-ID: 132824  Cd Length: 141  Bit Score: 51.60  E-value: 6.15e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIfnPQTVGV-GRARFTtyeVRMRTNLPIFKLKESCVRRRYSDFEWLKN---ELERDSKIVVPPLP------GKALK 99
Cdd:cd07291   3 LQIDI--PDALSErDKVKFT---VHTKTTLPSFQSPDFSVTRQHEDFIWLHDaliETEDYAGLIIPPAPpkpdfdGPREK 77
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 23111030 100 RQLPFRGDEGIFEESFiEERRQGLEQ---------------FINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd07291  78 MQKLGEGEGSMTKEEF-AKMKQELEAeylavfkktvqvhevFLQRLSSHPSLSKDRNFHIFLE 139
PX_HS1BP3 cd06868
The phosphoinositide binding Phox Homology domain of HS1BP3; The PX domain is a ...
32-146 1.93e-07

The phosphoinositide binding Phox Homology domain of HS1BP3; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Hematopoietic lineage cell-specific protein-1 (HS1) binding protein 3 (HS1BP3) associates with HS1 proteins through their SH3 domains, suggesting a role in mediating signaling. It has been reported that HS1BP3 might affect the IL-2 signaling pathway in hematopoietic lineage cells. Mutations in HS1BP3 may also be associated with familial Parkinson disease and essential tremor. HS1BP3 contains a PX domain, a leucine zipper, motifs similar to immunoreceptor tyrosine-based inhibitory motif and proline-rich regions. The PX domain interacts with PIs and plays a role in targeting proteins to PI-enriched membranes.


Pssm-ID: 132778  Cd Length: 120  Bit Score: 47.02  E-value: 1.93e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  32 IDIFNPQTVGVGRARFTT---YEVRMRTNLPIFK----LKESCVR----RRYSDFEWLKNEL-ERDSKIVVPPLPGKALk 99
Cdd:cd06868   2 LDLTVPEYQEIRGKTSSGhvlYQIVVVTRLAAFKsakhKEEDVVQfmvsKKYSEFEELYKKLsEKYPGTILPPLPRKAL- 80
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*..
gi 23111030 100 rqlpFRGdegifeESFIEERRQGLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd06868  81 ----FVS------ESDIRERRAAFNDFMRFISKDEKLANCPELLEFL 117
PX_MONaKA cd06871
The phosphoinositide binding Phox Homology domain of Modulator of Na,K-ATPase; The PX domain ...
68-135 2.66e-07

The phosphoinositide binding Phox Homology domain of Modulator of Na,K-ATPase; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. MONaKA (Modulator of Na,K-ATPase) binds the plasma membrane ion transporter, Na,K-ATPase, and modulates its enzymatic and ion pump activities. It modulates brain Na,K-ATPase and may be involved in regulating electrical excitability and synaptic transmission. MONaKA contains an N-terminal PX domain and a C-terminal catalytic kinase domain. The PX domain interacts with PIs and plays a role in targeting proteins to PI-enriched membranes.


Pssm-ID: 132781  Cd Length: 120  Bit Score: 46.97  E-value: 2.66e-07
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 23111030  68 VRRRYSDFEWLKNELERdSKIVVPpLPGKALKrqlpfrgdeGIFEESFIEERRQGLEQFINKIAGHPL 135
Cdd:cd06871  40 VIRRYNDFDLLNASLQI-SGISLP-LPPKKLI---------GNMDREFIAERQQGLQNYLNVILMNPI 96
PX_SNX14 cd06877
The phosphoinositide binding Phox Homology domain of Sorting Nexin 14; The PX domain is a ...
67-146 5.27e-07

The phosphoinositide binding Phox Homology domain of Sorting Nexin 14; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX14 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. It is expressed in the embryonic nervous system of mice, and is co-expressed in the motoneurons and the anterior pituary with Islet-1. SNX14 shows a similar domain architecture as SNX13, containing an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs.


Pssm-ID: 132787  Cd Length: 119  Bit Score: 45.83  E-value: 5.27e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  67 CVRRRYSDFEWLKNELE------RDSkivvpplpgkalkrQLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNER 140
Cdd:cd06877  45 SVLRRYNEFYVLESKLTefhgefPDA--------------PLPSRRIFGPKSYEFLESKREIFEEFLQKLLQKPELRGSE 110

                ....*.
gi 23111030 141 CLHMFL 146
Cdd:cd06877 111 LLYDFL 116
PX_SNX19 cd06893
The phosphoinositide binding Phox Homology domain of Sorting Nexin 19; The PX domain is a ...
47-146 1.04e-06

The phosphoinositide binding Phox Homology domain of Sorting Nexin 19; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX19 contains an N-terminal PXA domain, a central PX domain, and a C-terminal domain that is conserved in some SNXs. These domains are also found in SNX13 and SNX14, which also contain a regulator of G protein signaling (RGS) domain in between the PXA and PX domains. SNX19 interacts with IA-2, a major autoantigen found in type-1 diabetes. It inhibits the conversion of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] to PI(3,4,5)P3, which leads in the decrease of protein phosphorylation in the Akt signaling pathway, resulting in apoptosis. SNX19 may also be implicated in coronary heart disease and thyroid oncocytic tumors.


Pssm-ID: 132803 [Multi-domain]  Cd Length: 132  Bit Score: 45.61  E-value: 1.04e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMRTNL---PIFKLKESCVRRRYSDFEWLKNELERDSKI--VVPPLPGKALKRQLPFrgdeGIFEESFIEERRQ 121
Cdd:cd06893  29 ETILDVQSEQNPnaaSEQPLATHTVNRRFREFLTLQTRLEENPKFrkIMNVKGPPKRLFDLPF----GNMDKDKIEARRG 104
                        90       100
                ....*....|....*....|....*
gi 23111030 122 GLEQFINKIAGHPLAQNERCLHMFL 146
Cdd:cd06893 105 LLETFLRQLCSIPEISNSEEVQEFL 129
PX_SNX15_like cd06881
The phosphoinositide binding Phox Homology domain of Sorting Nexin 15-like proteins; The PX ...
33-148 5.61e-06

The phosphoinositide binding Phox Homology domain of Sorting Nexin 15-like proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily have similarity to sorting nexin 15 (SNX15), which contains an N-terminal PX domain and a C-terminal Microtubule Interacting and Trafficking (MIT) domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNX15 plays a role in protein trafficking processes in the endocytic pathway and the trans-Golgi network. The PX domain of SNX15 interacts with the PDGF receptor and is responsible for the membrane association of the protein. Other members of this subfamily contain an additional C-terminal kinase domain, similar to human RPK118, which binds sphingosine kinase and the antioxidant peroxiredoxin-3 (PRDX3). RPK118 may be involved in the transport of proteins such as PRDX3 from the cytoplasm to its site of function in the mitochondria.


Pssm-ID: 132791  Cd Length: 117  Bit Score: 43.08  E-value: 5.61e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  33 DIFNPQTVGVGrarFTTYEV--RMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIV-----VPPLP-GKALKRqlpf 104
Cdd:cd06881   6 TVTDTRRHKKG---YTEYKItsKVFSRSVPEDVSEVVVWKRYSDFKKLHRELSRLHKQLylsgsFPPFPkGKYFGR---- 78
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....
gi 23111030 105 rgdegiFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQE 148
Cdd:cd06881  79 ------FDAAVIEERRQAILELLDFVGNHPALYQSSAFQQFFEE 116
PX_SNX9 cd07285
The phosphoinositide binding Phox Homology domain of Sorting Nexin 9; The PX domain is a ...
68-146 1.43e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexin 9; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX9, also known as SH3PX1, is a cytosolic protein that interacts with proteins associated with clathrin-coated pits such as Cdc-42-associated tyrosine kinase 2 (ACK2). It contains an N-terminal Src Homology 3 (SH3) domain, a PX domain, and a C-terminal Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature. The PX-BAR structural unit helps determine specific membrane localization. Through its SH3 domain, SNX9 binds class I polyproline sequences found in dynamin 1/2 and the WASP/N-WASP actin regulators. SNX9 is localized to plasma membrane endocytic sites and acts primarily in clathrin-mediated endocytosis. Its array of interacting partners suggests that SNX9 functions at the interface between endocytosis and actin cytoskeletal organization.


Pssm-ID: 132818  Cd Length: 126  Bit Score: 42.32  E-value: 1.43e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  68 VRRRYSDFEWLKNEL--ERDSKIVVPPLPGKALKrqlpfrgdeGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMF 145
Cdd:cd07285  34 VNHRYKHFDWLYERLlvKFGLAIPIPSLPDKQVT---------GRFEEEFIKMRMERLQAWMTRMCRHPVISESEVFQQF 104

                .
gi 23111030 146 L 146
Cdd:cd07285 105 L 105
PX_SNX5_like cd06892
The phosphoinositide binding Phox Homology domain of Sorting Nexins 5 and 6; The PX domain is ...
50-149 1.95e-05

The phosphoinositide binding Phox Homology domain of Sorting Nexins 5 and 6; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Members of this subfamily include SNX5, SNX6, and similar proteins. They contain a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to other sorting nexins including SNX1-2. The PX-BAR structural unit helps determine the specific membrane-targeting of some SNXs. SNX5 and SNX6 may be components of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, acting as a mammalian equivalent of yeast Vsp17p.


Pssm-ID: 132802  Cd Length: 141  Bit Score: 42.04  E-value: 1.95e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  50 YEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSK---IVVPPLPGKA------LKRQLPFRGDEGIFEESFiEERR 120
Cdd:cd06892  19 FTVHTKTTLPTFQKPEFSVTRQHEEFVWLHDTLVENEDyagLIIPPAPPKPdfdasrEKLQKLGEGEGSMTKEEF-EKMK 97
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....
gi 23111030 121 QGLEQ---------------FINKIAGHPLAQNERCLHMFLQEE 149
Cdd:cd06892  98 QELEAeylaifkktvamhevFLRRLASHPVLRNDANFRVFLEYE 141
PX_CISK cd06870
The phosphoinositide binding Phox Homology Domain of Cytokine-Independent Survival Kinase; The ...
44-147 2.35e-05

The phosphoinositide binding Phox Homology Domain of Cytokine-Independent Survival Kinase; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Cytokine-independent survival kinase (CISK), also called Serum- and Glucocorticoid-induced Kinase 3 (SGK3), plays a role in cell growth and survival. It is expressed in most tissues and is most abundant in the embryo and adult heart and spleen. It was originally discovered in a screen for antiapoptotic genes. It phosphorylates and inhibits the proapoptotic proteins, Bad and FKHRL1. CISK/SGK3 also regulates many transporters, ion channels, and receptors. It plays a critical role in hair follicle morphogenesis and hair cycling. N-terminal to a catalytic kinase domain, CISK contains a PX domain which binds highly phosphorylated PIs, directs membrane localization, and regulates the enzyme's activity.


Pssm-ID: 132780  Cd Length: 109  Bit Score: 41.24  E-value: 2.35e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  44 RARFTTYEVRMRTNLpifklKESCVRRRYSDFEWLKNELERDskivvppLPGKALKrqLP---FRGDEgiFEESFIEERR 120
Cdd:cd06870  17 KKRFTVYKVVVSVGR-----SSWFVFRRYAEFDKLYESLKKQ-------FPASNLK--IPgkrLFGNN--FDPDFIKQRR 80
                        90       100
                ....*....|....*....|....*..
gi 23111030 121 QGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd06870  81 AGLDEFIQRLVSDPKLLNHPDVRAFLQ 107
PX_IRAS cd06875
The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor ...
68-130 5.56e-05

The phosphoinositide binding Phox Homology domain of the Imidazoline Receptor Antisera-Selected; The PX domain is a phosphoinositide binding (PI) module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Imidazoline Receptor Antisera-Selected (IRAS), also called nischarin, contains an N-terminal PX domain, leucine rich repeats, and a predicted coiled coil domain. The PX domain of IRAS binds to phosphatidylinositol-3-phosphate in membranes. Together with the coiled coil domain, it is essential for the localization of IRAS to endosomes. IRAS has been shown to interact with integrin and inhibit cell migration. Its interaction with alpha5 integrin causes a redistribution of the receptor from the cell surface to endosomal structures, suggesting that IRAS may function as a sorting nexin (SNX) which regulates the endosomal trafficking of integrin. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway.


Pssm-ID: 132785  Cd Length: 116  Bit Score: 40.34  E-value: 5.56e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 23111030  68 VRRRYSDFEWLKNELERDSKIVVPPLPGKALKrqlpfrgdeGIFEESFIEERRQGLEQFINKI 130
Cdd:cd06875  33 VKHRYSDFAELHDKLVAEHKVDKDLLPPKKLI---------GNKSPSFVEKRRKELEIYLQTL 86
PX_SNX6 cd07292
The phosphoinositide binding Phox Homology domain of Sorting Nexin 6; The PX domain is a ...
50-147 1.41e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexin 6; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX6 forms a stable complex with SNX1 and may be a component of the retromer complex, a membrane coat multimeric complex required for endosomal retrieval of lysosomal hydrolase receptors to the Golgi, acting as a mammalian equivalent of yeast Vsp17p. It interacts with the receptor serine/threonine kinases from the transforming growth factor-beta family. It also plays roles in enhancing the degradation of EGFR and in regulating the activity of Na,K-ATPase through its interaction with Translationally Controlled Tumor Protein (TCTP). SNX6 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to other sorting nexins including SNX1-2. The PX-BAR structural unit helps determine the specific membrane-targeting of some SNXs.


Pssm-ID: 132825  Cd Length: 141  Bit Score: 39.70  E-value: 1.41e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  50 YEVRMRTNLPIFKLKESCVRRRYSDFEWLKN---ELERDSKIVVPPLPGK----ALKRQLPFRGD-EGIFEESFIEERRQ 121
Cdd:cd07292  19 FTVHTKSSLPNFKQNEFSVVRQHEEFIWLHDsfvENEDYAGYIIPPAPPRpdfdASREKLQKLGEgEGSMTKEEFTKMKQ 98
                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 23111030 122 GLEQ---------------FINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd07292  99 ELEAeylaifkktvamhevFLCRVAAHPILRKDLNFHVFLE 139
PX_SNX20_21_like cd07279
The phosphoinositide binding Phox Homology domain of Sorting Nexins 20 and 21; The PX domain ...
30-147 4.13e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexins 20 and 21; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. This subfamily consists of SNX20, SNX21, and similar proteins. SNX20 interacts with P-Selectin glycoprotein ligand-1 (PSGL-1), a surface-expressed mucin that acts as a ligand for the selectin family of adhesion proteins. It may function in the sorting and cycling of PSGL-1 into endosomes. SNX21, also called SNX-L, is distinctly and highly-expressed in fetal liver and may be involved in protein sorting and degradation during embryonic liver development.


Pssm-ID: 132812  Cd Length: 112  Bit Score: 38.08  E-value: 4.13e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMRTNlPIFKLKESCVRRRYSDFEWLKNELERDskivvppLPGKALKRQLPFRGDEG 109
Cdd:cd07279   1 LKFEIVSARTVKEGEKKYVVYQLAVVQT-GDPDTQPAFIERRYSDFLKLYKALRKQ-------HPQLMAKVSFPRKVLMG 72
                        90       100       110
                ....*....|....*....|....*....|....*...
gi 23111030 110 IFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd07279  73 NFSSELIAERSRAFEQFLGHILSIPNLRDSKAFLDFLQ 110
PX_SNX15 cd07288
The phosphoinositide binding Phox Homology domain of Sorting Nexin 15; The PX domain is a ...
63-148 4.81e-04

The phosphoinositide binding Phox Homology domain of Sorting Nexin 15; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX15 contains an N-terminal PX domain and a C-terminal Microtubule Interacting and Trafficking (MIT) domain. It plays a role in protein trafficking processes in the endocytic pathway and the trans-Golgi network. The PX domain of SNX15 interacts with the PDGF receptor and is responsible for the membrane association of the protein.


Pssm-ID: 132821  Cd Length: 118  Bit Score: 38.03  E-value: 4.81e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  63 LKESCVRRRYSDFEWLKNELERDSKIVV------PPLPgkalkRQLPFrgdeGIFEESFIEERRQGLEQFINKIAGHPLA 136
Cdd:cd07288  35 VKEVVVWKRYSDLKKLHGELAYTHRNLFrrqeefPPFP-----RAQVF----GRFEAAVIEERRNAAEAMLLFTVNIPAL 105
                        90
                ....*....|..
gi 23111030 137 QNERCLHMFLQE 148
Cdd:cd07288 106 YNSPQLKEFFRD 117
PX_SNX17_31 cd06885
The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain ...
71-147 2.15e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexins 17 and 31; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Members of this subfamily include sorting nexin 17 (SNX17), SNX31, and similar proteins. They contain an N-terminal PX domain followed by a truncated FERM (4.1, ezrin, radixin, and moesin) domain and a unique C-terminal region. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX17 is known to regulate the trafficking and processing of a number of proteins. It binds some members of the low-density lipoprotein receptor (LDLR) family such as LDLR, VLDLR, ApoER2, and others, regulating their endocytosis. It also binds P-selectin and may regulate its lysosomal degradation. SNX17 is highly expressed in neurons. It binds amyloid precursor protein (APP) and may be involved in its intracellular trafficking and processing to amyloid beta peptide, which plays a central role in the pathogenesis of Alzheimer's disease. The biological function of SNX31 is unknown.


Pssm-ID: 132795  Cd Length: 104  Bit Score: 35.77  E-value: 2.15e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 23111030  71 RYSDFEWLKNELERD-SKIVVPPLPGKALKRQLPfrgdegifeeSFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd06885  34 RYSQLHGLNEQLKKEfGNRKLPPFPPKKLLPLTP----------AQLEERRLQLEKYLQAVVQDPRIANSDIFNSFLL 101
PX_SNX27 cd06886
The phosphoinositide binding Phox Homology domain of Sorting Nexin 27; The PX domain is a ...
30-130 2.73e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 27; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX27 contains an N-terminal PDZ domain followed by a PX domain and a Ras-Associated (RA) domain. It binds G protein-gated potassium (Kir3) channels, which play a role in neuronal excitability control, through its PDZ domain. SNX27 downregulates Kir3 channels by promoting their movement in the endosome, reducing surface expression and increasing degradation. SNX27 also associates with 5-hydroxytryptamine type 4 receptor (5-HT4R), cytohesin associated scaffolding protein (CASP), and diacylglycerol kinase zeta, and may play a role in their intracellular trafficking and endocytic recycling. The SNX27 PX domain preferentially binds to phosphatidylinositol-3-phosphate (PI3P) and is important for targeting to the early endosome.


Pssm-ID: 132796  Cd Length: 106  Bit Score: 35.46  E-value: 2.73e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  30 LEIDIFNPQTVGVGRARFTTYEVRMrtnlpifKLKESCvRRRYSDFEWLKNELERD-SKIVVPPLPGKalkrqLPFRgde 108
Cdd:cd06886   4 VPISIPDYKHVEQNGEKFVVYNIYM-------AGRQLC-SRRYREFANLHQNLKKEfPDFQFPKLPGK-----WPFS--- 67
                        90       100
                ....*....|....*....|..
gi 23111030 109 giFEESFIEERRQGLEQFINKI 130
Cdd:cd06886  68 --LSEQQLDARRRGLEQYLEKV 87
PX_SNX22 cd06880
The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a ...
67-134 4.58e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 22; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX22 may be involved in recruiting other proteins to the membrane via protein-protein and protein-ligand interaction. The biological function of SNX22 is not yet known.


Pssm-ID: 132790  Cd Length: 110  Bit Score: 34.94  E-value: 4.58e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 23111030  67 CVRRRYSDFEWLKNELERDskIVVPPLPGKALkRQLPFRGdegifeesfIEERRQGLEQFINKIAGHP 134
Cdd:cd06880  34 TVEKRYSEFHALHKKLKKS--IKTPDFPPKRV-RNWNPKV---------LEQRRQGLEAYLQGLLKIN 89
PX_PLD1 cd07296
The phosphoinositide binding Phox Homology domain of Phospholipase D1; The PX domain is a ...
68-138 5.40e-03

The phosphoinositide binding Phox Homology domain of Phospholipase D1; The PX domain is a phosphoinositide binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of phosphatidylcholine to generate membrane-bound phosphatidic acid and choline. PLDs are implicated in many cellular functions like signaling, cytoskeletal reorganization, vesicular transport, stress responses, and the control of differentiation, proliferation, and survival. PLD1 contains PX and Pleckstrin Homology (PH) domains in addition to the catalytic domain. It acts as an effector of Rheb in the signaling of the mammalian target of rapamycin (mTOR), a serine/threonine protein kinase that transduces nutrients and other stimuli to regulate many cellular processes. PLD1 also regulates the secretion of the procoagulant von Willebrand factor (VWF) in endothelial cells. The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. The PX domain of PLD1 specifically binds to phosphatidylinositol-3,4,5-trisphosphate [PI(3,4,5)P3], which enables PLD1 to mediate signals via the ERK1/2 pathway.


Pssm-ID: 132829  Cd Length: 135  Bit Score: 35.29  E-value: 5.40e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  68 VRRRYSDFEWLKNELERDSKIVVPPLPGKA--LKRQ------------LPFRGDEGIFEESFIEERRQgLEQFINKIAGH 133
Cdd:cd07296  41 VKRKFKHFQELHRELLRYKAFIRIPIPTRShtVRRQtikrgeprhmpsLPRGAEEEAREEQFSSRRKQ-LEDYLSKLLKM 119

                ....*
gi 23111030 134 PLAQN 138
Cdd:cd07296 120 PMYRN 124
PX_SNX13 cd06873
The phosphoinositide binding Phox Homology domain of Sorting Nexin 13; The PX domain is a ...
47-146 5.45e-03

The phosphoinositide binding Phox Homology domain of Sorting Nexin 13; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX13, also called RGS-PX1, contains an N-terminal PXA domain, a regulator of G protein signaling (RGS) domain, a PX domain, and a C-terminal domain that is conserved in some SNXs. It specifically binds to the stimulatory subunit of the heterotrimeric G protein G(alpha)s, serving as its GTPase activating protein, through the RGS domain. It preferentially binds phosphatidylinositol-3-phosphate (PI3P) through the PX domain and is localized in early endosomes. SNX13 is involved in endosomal sorting of EGFR into multivesicular bodies (MVB) for delivery to the lysosome.


Pssm-ID: 132783  Cd Length: 120  Bit Score: 34.94  E-value: 5.45e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMRTNLPIFKLKESCVRRRYSDF----EWLKNELERDSKIvvpPLPGKALKRQLpfrgdegifEESFIEERRQG 122
Cdd:cd06873  22 YAVYAISVTRIYPNGQEESWHVYRRYSDFhdlhMRLKEKFPNLSKL---SFPGKKTFNNL---------DRAFLEKRRKM 89
                        90       100       110
                ....*....|....*....|....*....|
gi 23111030 123 LEQFINKI------AGHPLAQNerCLHMFL 146
Cdd:cd06873  90 LNQYLQSLlnpevlDANPGLQE--IVLDFL 117
PX_RPK118_like cd07287
The phosphoinositide binding Phox Homology domain of RPK118-like proteins; The PX domain is a ...
47-147 6.47e-03

The phosphoinositide binding Phox Homology domain of RPK118-like proteins; The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. Members of this subfamily bear similarity to human RPK118, which contains an N-terminal PX domain, a Microtubule Interacting and Trafficking (MIT) domain, and a kinase domain. RPK118 binds sphingosine kinase, a key enzyme in the synthesis of sphingosine 1-phosphate (SPP), a lipid messenger involved in many cellular events. RPK118 may be involved in transmitting SPP-mediated signaling. It also binds the antioxidant peroxiredoxin-3 (PRDX3) and may be involved in the transport of PRDX3 from the cytoplasm to its site of function in the mitochondria. Members of this subfamily also show similarity to sorting nexin 15 (SNX15), which contains PX and MIT domains but does not contain a kinase domain. SNXs make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNX15 plays a role in protein trafficking processes in the endocytic pathway and the trans-Golgi network. The PX domain of SNX15 interacts with the PDGF receptor and is responsible for the membrane association of the protein.


Pssm-ID: 132820  Cd Length: 118  Bit Score: 34.94  E-value: 6.47e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 23111030  47 FTTYEVRMRT---NLPIfKLKESCVRRRYSDFEWLKNELE-------RDSKIVVPPLPGKALKRqlpfrgdegiFEESFI 116
Cdd:cd07287  17 YTVYKVTARIvsrKNPE-DVQEIVVWKRYSDFKKLHKDLWqihknlcRQSELFPPFAKAKVFGR----------FDESVI 85
                        90       100       110
                ....*....|....*....|....*....|.
gi 23111030 117 EERRQGLEQFINKIAGHPLAQNERCLHMFLQ 147
Cdd:cd07287  86 EERRQCAEDLLQFSANIPALYNSSQLEDFFK 116
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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