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Conserved domains on  [gi|1519311934|ref|NP_055534|]
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TRPM8 channel-associated factor 1 isoform 1 [Homo sapiens]

Protein Classification

M60 family metallopeptidase( domain architecture ID 13879573)

M60 family metallopeptidase similar to enhancin, a peptidase from granulosis viruses that affects Lepidoptera, and increases the potency of viral infection

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-889 3.81e-102

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


:

Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 318.94  E-value: 3.81e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 633 GAVHAPYYKLGETTLEEWKRRIQENPGPWGELATDNIILTVPTANLRTLE--NPEPLLRLWDEVMQAVARLGAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDrlNPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 707 PLRLPQRIVADVQISVGWMHAGYPIMCH-LESVQELINEKLIRTKGLWGPVHELGRNQQ-RQEWEFPPHTTEATCNLWCV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 785 YVHETVLGI---------PRSRANIALWPPVREKRVRIYLSKGPNVknwnawtaLETYLQLQEAFGWEPFIRLFTEYRNQ 855
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQLLYYFGWDFYRKLFRAYRTQ 232

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 1519311934 856 TNLPTENVD--KMNLWVKMFSHQVQKNLAPFFEAWA 889
Cdd:pfam13402 233 PYLPRNNSDgdKMSEFMLTFSKVAKRDLRPFFKAWG 268
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-631 1.22e-34

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


:

Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 127.84  E-value: 1.22e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 522 LRPSESPITVEVNctnpGTRYCWMSTGLYIPGRQIIEVSLPEAAAsADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDK 601
Cdd:pfam17291   1 NNATREKARVRIN----SRYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|
gi 1519311934 602 PTKSITCLWGGLLYIIVPQNSKLGSVPVTV 631
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTI 105
 
Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-889 3.81e-102

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 318.94  E-value: 3.81e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 633 GAVHAPYYKLGETTLEEWKRRIQENPGPWGELATDNIILTVPTANLRTLE--NPEPLLRLWDEVMQAVARLGAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDrlNPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 707 PLRLPQRIVADVQISVGWMHAGYPIMCH-LESVQELINEKLIRTKGLWGPVHELGRNQQ-RQEWEFPPHTTEATCNLWCV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 785 YVHETVLGI---------PRSRANIALWPPVREKRVRIYLSKGPNVknwnawtaLETYLQLQEAFGWEPFIRLFTEYRNQ 855
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQLLYYFGWDFYRKLFRAYRTQ 232

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 1519311934 856 TNLPTENVD--KMNLWVKMFSHQVQKNLAPFFEAWA 889
Cdd:pfam13402 233 PYLPRNNSDgdKMSEFMLTFSKVAKRDLRPFFKAWG 268
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-631 1.22e-34

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 127.84  E-value: 1.22e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 522 LRPSESPITVEVNctnpGTRYCWMSTGLYIPGRQIIEVSLPEAAAsADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDK 601
Cdd:pfam17291   1 NNATREKARVRIN----SRYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|
gi 1519311934 602 PTKSITCLWGGLLYIIVPQNSKLGSVPVTV 631
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTI 105
ImpA_fam_HExGH NF038322
ImpA family metalloprotease; Members of this family are metalloproteases related to the ImpA ...
 522-667 4.85e-05

ImpA family metalloprotease; Members of this family are metalloproteases related to the ImpA from Pseudomonas aeruginosa (PA0572 in strain PAO1), a virulence factor that cleaves CD44 on the surface of human macrophages, inhibiting phagocytosis. Members of this family are distinguished by a strong motif, CGxGCSGNPxD, just a few residues upstream of the familiar metalloprotease motif HExxH (typically HELGH here) and completely lacking in more distant homologs of ImpA.


Pssm-ID: 468474 [Multi-domain]  Cd Length: 879  Bit Score: 47.23  E-value: 4.85e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 522 LRPSESPITVEVNCTNPGTrycWMSTGLY-IPGrQIIEVSLpEAAASADLKIQIGCHTDDLTRA--SKLFRGPLVIN--R 596
Cdd:NF038322  392 LPPSTTPETKTVTLPSKSG---FTAIGRYaLPG-QTVTVTR-TDNSGATLAVFINTLRSGSTREfdKNGYNRPRFLQspR 466

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1519311934 597 CCLdKPTKSITcL---WGGLLYIIVPQNSKLGSVPVTVKGAVHAPYYKLGETTLE--EWKRRIQENPGPWGELATD 667
Cdd:NF038322  467 IPL-APGETVT-FtspYGGPLQLNFSGATAGLTVTLRFKGVAKHPHWRDFTDNAQiaAFVAALAAGDFDWAELKTP 540
 
Name Accession Description Interval E-value
Peptidase_M60 pfam13402
Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc ...
 633-889 3.81e-102

Peptidase M60, enhancin and enhancin-like; This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonization of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.


Pssm-ID: 433178 [Multi-domain]  Cd Length: 268  Bit Score: 318.94  E-value: 3.81e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 633 GAVHAPYYKLGETTLEEWKRRIQENPGPWGELATDNIILTVPTANLRTLE--NPEPLLRLWDEVMQAVARLGAEP----F 706
Cdd:pfam13402   1 GAVRAPYFVYGKTTDEEWEEMLRNYPAPWAELDGGRVILTVPSEFLRQLDrlNPQALWRLWDRVMYSVNEVAGLPrrsdG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 707 PLRLPQRIVADVQISVGWMHAGYPIMCH-LESVQELINEKLIRTKGLWGPVHELGRNQQ-RQEWEFPPHTTEATCNLWCV 784
Cdd:pfam13402  81 RVTRPVRFVFDRQISAGFMHAGYPIMAFpPGSASELVNYDSARKSGCWGPLHELGHNFQqRWGWTWPGHTGEVTNNILSL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 785 YVHETVLGI---------PRSRANIALWPPVREKRVRIYLSKGPNVknwnawtaLETYLQLQEAFGWEPFIRLFTEYRNQ 855
Cdd:pfam13402 161 YVQELLTGIdstrqinfnGRARWGPALGGWARDKHAKEYLNKGKDL--------LVFYGQLLYYFGWDFYRKLFRAYRTQ 232

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 1519311934 856 TNLPTENVD--KMNLWVKMFSHQVQKNLAPFFEAWA 889
Cdd:pfam13402 233 PYLPRNNSDgdKMSEFMLTFSKVAKRDLRPFFKAWG 268
M60-like_N pfam17291
N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.
 522-631 1.22e-34

N-terminal domain of M60-like peptidases; This accessory domain has a jelly roll topology.


Pssm-ID: 435844 [Multi-domain]  Cd Length: 106  Bit Score: 127.84  E-value: 1.22e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 522 LRPSESPITVEVNctnpGTRYCWMSTGLYIPGRQIIEVSLPEAAAsADLKIQIGCHTDDLTRASKLFRGPLVINRCCLDK 601
Cdd:pfam17291   1 NNATREKARVRIN----SRYSDWQSTGLYAPPGELITIEVPDNAV-GKLVVQIGCHTDNLGHADELFRPPVVTCRFPLDE 75

                          90       100       110
                  ....*....|....*....|....*....|
gi 1519311934 602 PTKSITCLWGGLLYIIVPQNSKLGSVPVTV 631
Cdd:pfam17291  76 GVNKISWPYGGLIYIIVPIDTFEGAVEVTI 105
ImpA_fam_HExGH NF038322
ImpA family metalloprotease; Members of this family are metalloproteases related to the ImpA ...
 522-667 4.85e-05

ImpA family metalloprotease; Members of this family are metalloproteases related to the ImpA from Pseudomonas aeruginosa (PA0572 in strain PAO1), a virulence factor that cleaves CD44 on the surface of human macrophages, inhibiting phagocytosis. Members of this family are distinguished by a strong motif, CGxGCSGNPxD, just a few residues upstream of the familiar metalloprotease motif HExxH (typically HELGH here) and completely lacking in more distant homologs of ImpA.


Pssm-ID: 468474 [Multi-domain]  Cd Length: 879  Bit Score: 47.23  E-value: 4.85e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1519311934 522 LRPSESPITVEVNCTNPGTrycWMSTGLY-IPGrQIIEVSLpEAAASADLKIQIGCHTDDLTRA--SKLFRGPLVIN--R 596
Cdd:NF038322  392 LPPSTTPETKTVTLPSKSG---FTAIGRYaLPG-QTVTVTR-TDNSGATLAVFINTLRSGSTREfdKNGYNRPRFLQspR 466

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1519311934 597 CCLdKPTKSITcL---WGGLLYIIVPQNSKLGSVPVTVKGAVHAPYYKLGETTLE--EWKRRIQENPGPWGELATD 667
Cdd:NF038322  467 IPL-APGETVT-FtspYGGPLQLNFSGATAGLTVTLRFKGVAKHPHWRDFTDNAQiaAFVAALAAGDFDWAELKTP 540
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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