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Conserved domains on  [gi|93141204|ref|NP_060866|]
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tRNA N(3)-methylcytidine methyltransferase METTL2B [Homo sapiens]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 10614797)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor

CATH:  2.20.25.110
EC:  2.1.-.-|2.1.1.-
Gene Ontology:  GO:0032259|GO:0008757|GO:0008168|GO:1904047
PubMed:  12504684|12826405

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 184-286 8.18e-18

Methyltransferase domain; This family appears to be a methyltransferase domain.


:

Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 77.60  E-value: 8.18e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   184 ILEVGCGVGNTVFPILQTnndPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFVL 263
Cdd:pfam13649   1 VLDLGCGTGRLTLALARR---GGARVTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGVL 73

                          90       100
                  ....*....|....*....|...
gi 93141204   264 SAVVPDKMQKAINRLSRLLKPGG 286
Cdd:pfam13649  74 HHLPDPDLEAALREIARVLKPGG 96
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 184-286 8.18e-18

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 77.60  E-value: 8.18e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   184 ILEVGCGVGNTVFPILQTnndPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFVL 263
Cdd:pfam13649   1 VLDLGCGTGRLTLALARR---GGARVTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGVL 73

                          90       100
                  ....*....|....*....|...
gi 93141204   264 SAVVPDKMQKAINRLSRLLKPGG 286
Cdd:pfam13649  74 HHLPDPDLEAALREIARVLKPGG 96
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 183-302 1.87e-15

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 72.72  E-value: 1.87e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTnndpGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFV 262
Cdd:COG2226  25 RVLDLGCGTGRLALALAER----GARVTGVDISPEMLELARERAAEAGLNVEFVVGDAED----LPFPDGSFDLVISSFV 96

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 93141204 263 LSAVvPDKmQKAINRLSRLLKPGGMVLLRDYGRYDMAQLR 302
Cdd:COG2226  97 LHHL-PDP-ERALAEIARVLKPGGRLVVVDFSPPDLAELE 134
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 183-290 2.63e-13

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 65.53  E-value: 2.63e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQtnnDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHdlCDEEKSYPVPKGSLDIIILIFV 262
Cdd:cd02440   1 RVLDLGCGTGALALALAS---GPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDPP 75

                        90       100
                ....*....|....*....|....*...
gi 93141204 263 LSAVVPDkMQKAINRLSRLLKPGGMVLL 290
Cdd:cd02440  76 LHHLVED-LARFLEEARRLLKPGGVLVL 102
PRK08317 PRK08317
hypothetical protein; Provisional
 183-292 8.12e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 46.47  E-value: 8.12e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204  183 RILEVGCGVGNTVFPILQTNNDPGLfVYCCDFSSTAIELVQTNSEYDPSRCFaFVHDlcDEEKSyPVPKGSLDIIILIFV 262
Cdd:PRK08317  22 RVLDVGCGPGNDARELARRVGPEGR-VVGIDRSEAMLALAKERAAGLGPNVE-FVRG--DADGL-PFPDGSFDAVRSDRV 96

                         90       100       110
                 ....*....|....*....|....*....|
gi 93141204  263 LSAvVPDKmQKAINRLSRLLKPGGMVLLRD 292
Cdd:PRK08317  97 LQH-LEDP-ARALAEIARVLRPGGRVVVLD 124
 
Name Accession Description Interval E-value
Methyltransf_25 pfam13649
Methyltransferase domain; This family appears to be a methyltransferase domain.
 184-286 8.18e-18

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 463945 [Multi-domain]  Cd Length: 96  Bit Score: 77.60  E-value: 8.18e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   184 ILEVGCGVGNTVFPILQTnndPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFVL 263
Cdd:pfam13649   1 VLDLGCGTGRLTLALARR---GGARVTGVDLSPEMLERARERAAEAGLNVEFVQGDAED----LPFPDGSFDLVVSSGVL 73

                          90       100
                  ....*....|....*....|...
gi 93141204   264 SAVVPDKMQKAINRLSRLLKPGG 286
Cdd:pfam13649  74 HHLPDPDLEAALREIARVLKPGG 96
Methyltransf_12 pfam08242
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 185-288 2.87e-16

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 400515 [Multi-domain]  Cd Length: 98  Bit Score: 73.56  E-value: 2.87e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   185 LEVGCGVGNTVFPILQTNndPGLFVYCCDFSSTAIELV-QTNSEYDPSRCFAFVHDLCDEEKSYPvpkGSLDIIILIFVL 263
Cdd:pfam08242   1 LEIGCGTGTLLRALLEAL--PGLEYTGLDISPAALEAArERLAALGLLNAVRVELFQLDLGELDP---GSFDVVVASNVL 75

                          90       100
                  ....*....|....*....|....*
gi 93141204   264 SAVvpDKMQKAINRLSRLLKPGGMV 288
Cdd:pfam08242  76 HHL--ADPRAVLRNIRRLLKPGGVL 98
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 183-302 1.87e-15

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 72.72  E-value: 1.87e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTnndpGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFV 262
Cdd:COG2226  25 RVLDLGCGTGRLALALAER----GARVTGVDISPEMLELARERAAEAGLNVEFVVGDAED----LPFPDGSFDLVISSFV 96

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 93141204 263 LSAVvPDKmQKAINRLSRLLKPGGMVLLRDYGRYDMAQLR 302
Cdd:COG2226  97 LHHL-PDP-ERALAEIARVLKPGGRLVVVDFSPPDLAELE 134
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 183-290 4.85e-15

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 73.03  E-value: 4.85e-15
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTNNDPglfVYCCDFSSTAIELVQTN-SEYDPSRCFAFVHDLCDEEksyPVPKGSLDIIILIF 261
Cdd:COG0500  29 RVLDLGCGTGRNLLALAARFGGR---VIGIDLSPEAIALARARaAKAGLGNVEFLVADLAELD---PLPAESFDLVVAFG 102

                        90       100
                ....*....|....*....|....*....
gi 93141204 262 VLSAVVPDKMQKAINRLSRLLKPGGMVLL 290
Cdd:COG0500 103 VLHHLPPEEREALLRELARALKPGGVLLL 131
AdoMet_MTases cd02440
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 183-290 2.63e-13

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


Pssm-ID: 100107 [Multi-domain]  Cd Length: 107  Bit Score: 65.53  E-value: 2.63e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQtnnDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHdlCDEEKSYPVPKGSLDIIILIFV 262
Cdd:cd02440   1 RVLDLGCGTGALALALAS---GPGARVTGVDISPVALELARKAAAALLADNVEVLK--GDAEELPPEADESFDVIISDPP 75

                        90       100
                ....*....|....*....|....*...
gi 93141204 263 LSAVVPDkMQKAINRLSRLLKPGGMVLL 290
Cdd:cd02440  76 LHHLVED-LARFLEEARRLLKPGGVLVL 102
UbiG COG2227
2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and ...
 183-293 1.57e-11

2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and metabolism]; 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 441829 [Multi-domain]  Cd Length: 126  Bit Score: 61.19  E-value: 1.57e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTnndpGLFVYCCDFSSTAIELVQTNseYDPSRCFAFVHDLCDeeksYPVPKGSLDIIILIFV 262
Cdd:COG2227  27 RVLDVGCGTGRLALALARR----GADVTGVDISPEALEIARER--AAELNVDFVQGDLED----LPLEDGSFDLVICSEV 96

                        90       100       110
                ....*....|....*....|....*....|.
gi 93141204 263 LsAVVPDkMQKAINRLSRLLKPGGMVLLRDY 293
Cdd:COG2227  97 L-EHLPD-PAALLRELARLLKPGGLLLLSTP 125
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 185-290 2.95e-11

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 59.22  E-value: 2.95e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   185 LEVGCGVGNTVFPILQTnndpGLFVYCCDFSSTAIELVQTNSEYDPsrcFAFVHdlCDEEKSyPVPKGSLDIIILIFVLS 264
Cdd:pfam08241   1 LDVGCGTGLLTELLARL----GARVTGVDISPEMLELAREKAPREG---LTFVV--GDAEDL-PFPDNSFDLVLSSEVLH 70

                          90       100
                  ....*....|....*....|....*.
gi 93141204   265 AVvpDKMQKAINRLSRLLKPGGMVLL 290
Cdd:pfam08241  71 HV--EDPERALREIARVLKPGGILII 94
Methyltransf_23 pfam13489
Methyltransferase domain; This family appears to be a methyltransferase domain.
 183-343 4.45e-11

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 404385 [Multi-domain]  Cd Length: 162  Bit Score: 60.90  E-value: 4.45e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   183 RILEVGCGVGNtVFPILQTNNdpglfvyccdFSSTAIELvqtnSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFV 262
Cdd:pfam13489  25 RVLDFGCGTGI-FLRLLRAQG----------FSVTGVDP----SPIAIERALLNVRFDQFDEQEAAVPAGKFDVIVAREV 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   263 LSAVvPDkMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKgqclsgNFYVRGDGTRVYFFTQEELDTLFTTAGLEKV 342
Cdd:pfam13489  90 LEHV-PD-PPALLRQIAALLKPGGLLLLSTPLASDEADRLLLE------WPYLRPRNGHISLFSARSLKRLLEEAGFEVV 161


                  .
gi 93141204   343 Q 343
Cdd:pfam13489 162 S 162
Tam COG4106
Trans-aconitate methyltransferase [Energy production and conversion];
183-290 4.78e-08

Trans-aconitate methyltransferase [Energy production and conversion];


Pssm-ID: 443282 [Multi-domain]  Cd Length: 100  Bit Score: 50.59  E-value: 4.78e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTNndPGLFVYCCDFSSTAIELVQTNSeydpSRCFAFVHDLCDEEksypvPKGSLDIIILIFV 262
Cdd:COG4106   4 RVLDLGCGTGRLTALLAERF--PGARVTGVDLSPEMLARARARL----PNVRFVVADLRDLD-----PPEPFDLVVSNAA 72

                        90       100
                ....*....|....*....|....*...
gi 93141204 263 LSAVvPDkMQKAINRLSRLLKPGGMVLL 290
Cdd:COG4106  73 LHWL-PD-HAALLARLAAALAPGGVLAV 98
COG4976 COG4976
Predicted methyltransferase, contains TPR repeat [General function prediction only];
183-340 4.94e-08

Predicted methyltransferase, contains TPR repeat [General function prediction only];


Pssm-ID: 444001 [Multi-domain]  Cd Length: 181  Bit Score: 52.31  E-value: 4.94e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTnndpGLFVYCCDFSSTAIELVQTNSEYDPsrcfAFVHDLCDeeksYPVPKGSLDIIILIFV 262
Cdd:COG4976  49 RVLDLGCGTGLLGEALRPR----GYRLTGVDLSEEMLAKAREKGVYDR----LLVADLAD----LAEPDGRFDLIVAADV 116

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 93141204 263 LSAVvpDKMQKAINRLSRLLKPGGMVLlrdygrydmaqlrfkkgqclsgnFYV-RGDGTRVYFFTQEELDTLFTTAGLE 340
Cdd:COG4976 117 LTYL--GDLAAVFAGVARALKPGGLFI-----------------------FSVeDADGSGRYAHSLDYVRDLLAAAGFE 170
Methyltransf_31 pfam13847
Methyltransferase domain; This family appears to have methyltransferase activity.
 182-293 7.27e-07

Methyltransferase domain; This family appears to have methyltransferase activity.


Pssm-ID: 463998 [Multi-domain]  Cd Length: 150  Bit Score: 48.18  E-value: 7.27e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204   182 YRILEVGCGVGNTVFPILQTNNDPGlFVYCCDFSSTAIELVQTNSEydpSRCFAFVH-DLCD-EEKSYPVPKGSLDIIIL 259
Cdd:pfam13847   5 MRVLDLGCGTGHLSFELAEELGPNA-EVVGIDISEEAIEKARENAQ---KLGFDNVEfEQGDiEELPELLEDDKFDVVIS 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 93141204   260 IFVLSAVvPDKmQKAINRLSRLLKPGGMVLLRDY 293
Cdd:pfam13847  81 NCVLNHI-PDP-DKVLQEILRVLKPGGRLIISDP 112
Cfa COG2230
Cyclopropane fatty-acyl-phospholipid synthase and related methyltransferases [Lipid transport ...
 183-292 1.05e-06

Cyclopropane fatty-acyl-phospholipid synthase and related methyltransferases [Lipid transport and metabolism];


Pssm-ID: 441831 [Multi-domain]  Cd Length: 158  Bit Score: 48.00  E-value: 1.05e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204 183 RILEVGCGVGNTVFPILQTNndpGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVH--DLCDEEksypvPKGSLDIIILI 260
Cdd:COG2230  54 RVLDIGCGWGGLALYLARRY---GVRVTGVTLSPEQLEYARERAAEAGLADRVEVRlaDYRDLP-----ADGQFDAIVSI 125

                        90       100       110
                ....*....|....*....|....*....|..
gi 93141204 261 FVLSAVVPDKMQKAINRLSRLLKPGGMVLLRD 292
Cdd:COG2230 126 GMFEHVGPENYPAYFAKVARLLKPGGRLLLHT 157
PRK08317 PRK08317
hypothetical protein; Provisional
 183-292 8.12e-06

hypothetical protein; Provisional


Pssm-ID: 181382 [Multi-domain]  Cd Length: 241  Bit Score: 46.47  E-value: 8.12e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 93141204  183 RILEVGCGVGNTVFPILQTNNDPGLfVYCCDFSSTAIELVQTNSEYDPSRCFaFVHDlcDEEKSyPVPKGSLDIIILIFV 262
Cdd:PRK08317  22 RVLDVGCGPGNDARELARRVGPEGR-VVGIDRSEAMLALAKERAAGLGPNVE-FVRG--DADGL-PFPDGSFDAVRSDRV 96

                         90       100       110
                 ....*....|....*....|....*....|
gi 93141204  263 LSAvVPDKmQKAINRLSRLLKPGGMVLLRD 292
Cdd:PRK08317  97 LQH-LEDP-ARALAEIARVLRPGGRVVVLD 124
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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