TFIIS (transcription elongation factor S-II) N-terminal domain-containing protein contains structural motifs that are responsible for interacting with RNA polymerase II (RNAPII) and mediating its catalytic activities
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the ...
407-586
1.71e-99
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the Mediator complex in eukaryotes. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator. The C-terminal domain is critical and sufficient for its assembly into Mediator and its interaction with Pol II. The most highly conserved C-terminal amino acids are critical for these interactions because deletion of the last eight amino acids from the Med26 C terminus disrupted binding to Mediator and Pol II.
:
Pssm-ID: 464806 Cd Length: 182 Bit Score: 300.04 E-value: 1.71e-99
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of ...
178-405
1.82e-83
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of Mediator. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator.
:
Pssm-ID: 464807 [Multi-domain] Cd Length: 255 Bit Score: 261.35 E-value: 1.82e-83
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the ...
407-586
1.71e-99
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the Mediator complex in eukaryotes. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator. The C-terminal domain is critical and sufficient for its assembly into Mediator and its interaction with Pol II. The most highly conserved C-terminal amino acids are critical for these interactions because deletion of the last eight amino acids from the Med26 C terminus disrupted binding to Mediator and Pol II.
Pssm-ID: 464806 Cd Length: 182 Bit Score: 300.04 E-value: 1.71e-99
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of ...
178-405
1.82e-83
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of Mediator. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator.
Pssm-ID: 464807 [Multi-domain] Cd Length: 255 Bit Score: 261.35 E-value: 1.82e-83
N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a ...
14-85
2.00e-17
N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme
Pssm-ID: 238107 Cd Length: 76 Bit Score: 76.96 E-value: 2.00e-17
TFIIS helical bundle-like domain; Mediator is a large complex of up to 33 proteins that is ...
33-84
7.26e-14
TFIIS helical bundle-like domain; Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species {1-2]. It is arranged into four different sections, a core, a head, a tail and a kinase-activity part, and the number of subunits within each of these is what varies with species. Overall, Mediator regulates the transcriptional activity of RNA polymerase II but it would appear that each of the four different sections has a slightly different function. Mediator exists in two major forms in human cells: a smaller form that interacts strongly with pol II and activates transcription, and a large form that does not interact strongly with pol II and does not directly activate transcription. Notably, the 'small' and 'large' Mediator complexes differ in their subunit composition: the Med26 subunit preferentially associates with the small, active complex, whereas cdk8, cyclin C, Med12 and Med13 associate with the large Mediator complex. This family includesthe C terminal region of a number of eukaryotic hypothetical proteins which are homologous to the Saccharomyces cerevisiae protein IWS1. IWS1 is known to be an Pol II transcription elongation factor and interacts with Spt6 and Spt5.
Pssm-ID: 462573 [Multi-domain] Cd Length: 52 Bit Score: 66.00 E-value: 7.26e-14
transcription elongation factor S-II; This model represents eukaryotic transcription ...
14-89
2.62e-09
transcription elongation factor S-II; This model represents eukaryotic transcription elongation factor S-II. This protein allows stalled RNA transcription complexes to perform a cleavage of the nascent RNA and restart at the newly generated 3-prime end.
Pssm-ID: 273592 [Multi-domain] Cd Length: 299 Bit Score: 58.70 E-value: 2.62e-09
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the ...
407-586
1.71e-99
Mediator complex subunit 26 C-terminal; Med26_C is the C-terminal domain of subunit 26 of the Mediator complex in eukaryotes. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator. The C-terminal domain is critical and sufficient for its assembly into Mediator and its interaction with Pol II. The most highly conserved C-terminal amino acids are critical for these interactions because deletion of the last eight amino acids from the Med26 C terminus disrupted binding to Mediator and Pol II.
Pssm-ID: 464806 Cd Length: 182 Bit Score: 300.04 E-value: 1.71e-99
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of ...
178-405
1.82e-83
Mediator complex subunit 26 middle domain; Med26_M is the middle domain of subunit 26 of Mediator. Med19 and Med26 act synergistically to mediate the interaction between REST (a Kruppel-type zinc finger transcription factor that binds to a 21-bp RE1 silencing element present in over 900 human genes) and Mediator.
Pssm-ID: 464807 [Multi-domain] Cd Length: 255 Bit Score: 261.35 E-value: 1.82e-83
N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a ...
14-85
2.00e-17
N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme
Pssm-ID: 238107 Cd Length: 76 Bit Score: 76.96 E-value: 2.00e-17
TFIIS helical bundle-like domain; Mediator is a large complex of up to 33 proteins that is ...
33-84
7.26e-14
TFIIS helical bundle-like domain; Mediator is a large complex of up to 33 proteins that is conserved from plants to fungi to humans - the number and representation of individual subunits varying with species {1-2]. It is arranged into four different sections, a core, a head, a tail and a kinase-activity part, and the number of subunits within each of these is what varies with species. Overall, Mediator regulates the transcriptional activity of RNA polymerase II but it would appear that each of the four different sections has a slightly different function. Mediator exists in two major forms in human cells: a smaller form that interacts strongly with pol II and activates transcription, and a large form that does not interact strongly with pol II and does not directly activate transcription. Notably, the 'small' and 'large' Mediator complexes differ in their subunit composition: the Med26 subunit preferentially associates with the small, active complex, whereas cdk8, cyclin C, Med12 and Med13 associate with the large Mediator complex. This family includesthe C terminal region of a number of eukaryotic hypothetical proteins which are homologous to the Saccharomyces cerevisiae protein IWS1. IWS1 is known to be an Pol II transcription elongation factor and interacts with Spt6 and Spt5.
Pssm-ID: 462573 [Multi-domain] Cd Length: 52 Bit Score: 66.00 E-value: 7.26e-14
transcription elongation factor S-II; This model represents eukaryotic transcription ...
14-89
2.62e-09
transcription elongation factor S-II; This model represents eukaryotic transcription elongation factor S-II. This protein allows stalled RNA transcription complexes to perform a cleavage of the nascent RNA and restart at the newly generated 3-prime end.
Pssm-ID: 273592 [Multi-domain] Cd Length: 299 Bit Score: 58.70 E-value: 2.62e-09
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
of the residues that compose this conserved feature have been mapped to the query sequence.
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