NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|13569934|ref|NP_112222|]
View 

sperm acrosome membrane-associated protein 1 precursor [Homo sapiens]

Protein Classification

SPACA1 domain-containing protein( domain architecture ID 10194958)

SPACA1 domain-containing protein

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 31-275 6.60e-155

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


:

Pssm-ID: 260101  Cd Length: 248  Bit Score: 432.32  E-value: 6.60e-155
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934  31 NVTAAVQDAGLAHEGEGEEE--TENNDSETAENY-APPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESK 107
Cdd:cd13783   1 NVTALQDDVQLAEEASEEEGeeEEENESEVPENPtEAEAEEEVSNRTVVKEVEFGMCTVTCGIGIREVILTNGCPGGESK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 108 CVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLD 187
Cdd:cd13783  81 CVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVILPNDSAVLEVHRETHPVAFECDTLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 188 NNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSV 267
Cdd:cd13783 161 NNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIIINWAAVKSFWGSKASTTEIQSELSSM 240


                ....*...
gi 13569934 268 RYKDSTSL 275
Cdd:cd13783 241 RYKDSTSL 248
 
Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 31-275 6.60e-155

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


Pssm-ID: 260101  Cd Length: 248  Bit Score: 432.32  E-value: 6.60e-155
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934  31 NVTAAVQDAGLAHEGEGEEE--TENNDSETAENY-APPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESK 107
Cdd:cd13783   1 NVTALQDDVQLAEEASEEEGeeEEENESEVPENPtEAEAEEEVSNRTVVKEVEFGMCTVTCGIGIREVILTNGCPGGESK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 108 CVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLD 187
Cdd:cd13783  81 CVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVILPNDSAVLEVHRETHPVAFECDTLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 188 NNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSV 267
Cdd:cd13783 161 NNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIIINWAAVKSFWGSKASTTEIQSELSSM 240


                ....*...
gi 13569934 268 RYKDSTSL 275
Cdd:cd13783 241 RYKDSTSL 248
 
Name Accession Description Interval E-value
SPACA1 cd13783
Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is ...
 31-275 6.60e-155

Sperm acrosome membrane-associated protein 1; SPACA1 (aka SAMP32, due to its 32kDa M.W.) is localized to the acrosome of spermatozoa. The acrosome is an organelle transformed from the Golgi apparatus to form a cap over the anterior portion of the spermatozoa head, which contains the sperm nucleus. Mammalian acrosomes contain digestive enzymes that degrade the ovum outer membrane (zona pellucida) to allow fusion of the sperm and ovum nuclei via the acrosomal reaction. In mammals, the acrosome releases hyaluronidase and acrosin. Antibodies generated against recombinant SPACA1 have been shown to inhibit human sperm binding and membrane fusion in vitro vs. zona-free hamster ova. Male mice lacking SPACA1 are infertile, and exhibit globozoospermia-like misformed sperm heads. SPACA1 content has been reported to be diminished in a comparison of round-headed vs normal spermatozoa.


Pssm-ID: 260101  Cd Length: 248  Bit Score: 432.32  E-value: 6.60e-155
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934  31 NVTAAVQDAGLAHEGEGEEE--TENNDSETAENY-APPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESK 107
Cdd:cd13783   1 NVTALQDDVQLAEEASEEEGeeEEENESEVPENPtEAEAEEEVSNRTVVKEVEFGMCTVTCGIGIREVILTNGCPGGESK 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 108 CVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLD 187
Cdd:cd13783  81 CVVRVEECRGPVDCGWGKPISESLSSVRLACIHISPENRFKYVWKLLRPDQQAVILPNDSAVLEVHRETHPVAFECDTLD 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13569934 188 NNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVIICVFIIFLLIFIIINWAAVKAFWGAKASTPEVQSEQSSV 267
Cdd:cd13783 161 NNEIVASVKFTVYTTSELQMRRSSRPDTDAVLIFVLTIGVIICIFVIFVLIFIIINWAAVKSFWGSKASTTEIQSELSSM 240


                ....*...
gi 13569934 268 RYKDSTSL 275
Cdd:cd13783 241 RYKDSTSL 248
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH