NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|42713696|ref|NP_116182|]
View 

sodium-dependent lysophosphatidylcholine symporter 1 isoform 2 [Homo sapiens]

Protein Classification

sodium-dependent lysophosphatidylcholine symporter 1( domain architecture ID 13024589)

sodium-dependent lysophosphatidylcholine symporter 1 (NLS1) plays an essential role for blood-brain barrier formation and function

CATH:  1.20.1250.20
Gene Ontology:  GO:0051978|GO:0051977|GO:0016020
PubMed:  23177091|26098515|26758938
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 46-496 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 341009  Cd Length: 419  Bit Score: 745.10  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 125
Cdd:cd17451   1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 205
Cdd:cd17451  81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 206 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 285
Cdd:cd17451 161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 286 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 365
Cdd:cd17451 209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 366 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17451 289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|.
gi 42713696 446 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17451 369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
 
Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 46-496 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341009  Cd Length: 419  Bit Score: 745.10  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 125
Cdd:cd17451   1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 205
Cdd:cd17451  81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 206 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 285
Cdd:cd17451 161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 286 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 365
Cdd:cd17451 209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 366 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17451 289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|.
gi 42713696 446 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17451 369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 48-496 1.25e-96

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 299.61  E-value: 1.25e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696    48 CYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTcLGRLMPWIIFSTPLAV 127
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTR-WGRRRPWLLLSAIILA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   128 IAYFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVG 206
Cdd:pfam13347  80 VSFILLFTPPELgRAPLFIWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAFPLVL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   207 qadtpcfqdlnsstvasqsanhthGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVRE---QREPYEAQQSEPIAYFR 283
Cdd:pfam13347 160 ------------------------ILGGTGLERKGYRIFALIGAVLMLLGVIITAAGTKErvsMRSKEDTGQKEAGSLLD 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   284 GLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVG 363
Cdd:pfam13347 216 MLKEVFRNRAFLILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAASAFPLVFTIGALLGIPLWPPLAKRIGKKNTYILG 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   364 ISSAVPFLILVALM-ESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEpiFFSFYVFFTKFASGVS 442
Cdd:pfam13347 296 ALITIAGFALALLLgPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGT--FFAMWSFISKLATGVG 373

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....
gi 42713696   443 LGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:pfam13347 374 LGVSGLLLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
41-512 5.22e-77

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 249.43  E-value: 5.22e-77
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  41 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWII 120
Cdd:COG2211   7 LSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGA-LSDRTRTRWGRRRPWIL 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 121 FSTPLAVIAYFLIWFVPDFPHGQTYWYLL-FYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTA 199
Cdd:COG2211  86 IGAIPLALSFVLLFTAPDLSPTGKLIYALvTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFAGLGGLLASV 165

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 200 IQGQIVGQADTpcfqdlnsstvasqsanhthgttshrETQKAYLLAAGVIVCIYIICAVILILGVREQREPyeaqQSEPI 279
Cdd:COG2211 166 LPPPLVAAFGG--------------------------DAALGYRLTALIFAVLGLLAFLLTFFGTKERPVP----EEEKV 215

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 280 AYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKT 358
Cdd:COG2211 216 SLKESLKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLsAALVGLLLALYFLAALLGAPLWPRLAKRFGKKK 295

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 359 AVYVGIS-SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKF 437
Cdd:COG2211 296 AFIIGLLlAALGLLLLFFLGPGNLWLLLVLAALAGIGLGAILVLPWAMLADVVDYDEWK--TGRRREGLYFGIFTFAIKL 373

                       410       420       430       440       450       460       470
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 42713696 438 ASGVSLGISTLSLDFAGYQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDE 512
Cdd:COG2211 374 GQALAGALAGLLLALFGYVA-GAAQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARRAA 447
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 46-501 5.65e-30

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 121.97  E-value: 5.65e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696    46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPWI-IFS 122
Cdd:TIGR00792   1 KLSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVdrTRTRW---GKFRPWLlIGA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   123 TPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMtvevlgtvLGTAIQ 201
Cdd:TIGR00792  78 IPFSIVL-VLLFTTPDFsGTGKLVYAYITYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRR--------FGATLG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   202 GQIVGQADTPcfqdlnsSTVASQSANHTHGttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAY 281
Cdd:TIGR00792 149 GLLVAVIVLP-------LVSYFGGGDDKFG----------WFMFALVLALIGVVSLIICFFGTKERYSEIPKNIEKKLSL 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   282 FRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVY 361
Cdd:TIGR00792 212 KQIFKALFKNDQLLILCLAYLFYNLAFNIKNGVQVYYFTYVLGDPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRKILFA 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   362 VGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPhfHGTEPIFFSFYVFFTKFASGV 441
Cdd:TIGR00792 292 GGILLMVLGYLIFFFAGSNLPLILVLIILAGFGQNIVTGLVWALVADTVDYGEWKTG--IRAEGLVYSVRTFVRKLGQAL 369

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 42713696   442 SLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLF-KMYPIDEERRRQ 501
Cdd:TIGR00792 370 AGFFVGLILGIIGY-VANAAQSPITLNGIKILMFAVPALFLLLAAIIIgRFYKLTEKKHAE 429
PRK09669 PRK09669
putative symporter YagG; Provisional
 40-498 9.26e-18

putative symporter YagG; Provisional


Pssm-ID: 236610  Cd Length: 444  Bit Score: 85.55  E-value: 9.26e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   40 QLSVCNKLCYALGGapyqvTGCAL-----GFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGR 114
Cdd:PRK09669   5 QLTTKDKIGYGLGD-----TACNLvwqtvMLFLAYFYTDVFGLSAAIMGTMFLVVRVLDAVTDPLMGALVDRTR-TRHGQ 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  115 LMPWII-FSTPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVL 192
Cdd:PRK09669  79 FRPYLLwFAIPFGVVC-LLTFYTPDFgATGKIIYACVTYILLSLVYTAINVPYCAMPGAITNDPRERHSLQSWRFALSFI 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  193 GTVLGTAIQGQIV---GQADtpcfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQRE 269
Cdd:PRK09669 158 GGLIVSVIALPLVdilGKGD----------------------------EQKGYFYAMMVMGLLGVVLFFCCFFMTKERYT 209

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  270 PYEAQQSepiAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQW 349
Cdd:PRK09669 210 PEVDNSS---SVWKDLKLLLGNSQWRIMFIFNVVLLTAVVTRGGATLYYVNYVLLRPDLATLFLVTGMIAGLFGALLSER 286

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  350 FLTRFGKKTAVYVGISSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfHLKQPHFHGTEPIFF 428
Cdd:PRK09669 287 LLGKFDRVRAFKWTIVAFVILSALIFFIPpSNVWLIFALNILFNFIQNLTTPLQWSMFSDVVD--YEEKRSGRRLDGLVF 364

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 42713696  429 SFYVFFTKFasGVSLGISTL--SLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEER 498
Cdd:PRK09669 365 STNLFAIKL--GLAIGGAVVgwILAWVDYVGGAAVQSASVLTTINLLFTVIPGVLFAGMAIILNFYKLNDAR 434
 
Name Accession Description Interval E-value
MFS_NLS1_MFSD2A cd17451
Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of ...
 46-496 0e+00

Sodium-dependent lysophosphatidylcholine symporter 1 of the Major Facilitator Superfamily of transporters; Sodium-dependent lysophosphatidylcholine (LPC) symporter 1 (NLS1) is also called major facilitator superfamily domain-containing protein 2A (MFSD2A). NLS1/MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. It also transports the essential omega-3 fatty acid docosahexaenoic acid (DHA), which is essential for normal brain growth and cognitive function, in the form of LPC into the brain across the blood-brain barrier. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. NLS1/MFSD2A belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341009  Cd Length: 419  Bit Score: 745.10  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 125
Cdd:cd17451   1 KLCYAIGGAPYQITGCALGFFLQIYLLDVAQLDPFYASIILFVGRAWDAITDPTVGFFVSKSPWTRFGRLMPWIIFSTPF 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 205
Cdd:cd17451  81 AVLSYFLIWFVPDFSQGKVMWYLLFYCLFQTLQTCFHVPYSALTMFISTEQKERDSATAYRMTVEVLGTVLGTAIQGQIV 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 206 GQAdtpcfqdlnsstvasqsanhthgttshretqkAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 285
Cdd:cd17451 161 GMA--------------------------------AYMIAAGVICAIYVLCAIILFLGVREQREPCELKSQKPVSFFKGL 208

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 286 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 365
Cdd:cd17451 209 KLVMSHGPYIKLITGFLFTSLAFMLLEGNFALFCTYTLGFRNDFQNILLVIMLSATLTIPFWQWFLTRFGKKTAVYIGIS 288

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 366 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17451 289 SAVPFLILVVLVESNLIVTYVVSVAAGVSVAAAFLLPWSMLPDVVDDFKLKNPDSQGHEAIFYSFYVFFTKFASGVSLGI 368

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|.
gi 42713696 446 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17451 369 STLSLDFAGYQTRGCSQPEEVNLTLKMLVSAAPVVLILLGLLLFKLYPIDE 419
MFS_MFSD2 cd17392
Major facilitator superfamily domain-containing protein 2 subfamily; The major facilitator ...
 46-496 0e+00

Major facilitator superfamily domain-containing protein 2 subfamily; The major facilitator superfamily domain-containing protein 2 (MFSD2) subfamily is composed of two vertebrate members, MFSD2A amd MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. The MFSD2 subfamily belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340950  Cd Length: 446  Bit Score: 740.82  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 125
Cdd:cd17392   1 KLCYAIGGAPYQMTSSATGFFLQIFLLDVAQMGAFSVSLILFVGRVWDAVTDPLVGYFISRSQRTGIGRLMPWIVFSTPL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 205
Cdd:cd17392  81 IILAYFFLWFLPPFTSLSGLWYLTFYCLFETFMTCFHVPYSALTMFLGGCQRERDSATAYRMTVEVFGTLMGATIQGQIV 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 206 GQADTPCFQDLNSSTVASqsanhTHGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 285
Cdd:cd17392 161 GVAHRPRRQDCTATPGTS-----DHPVTVLPNTRRAYLIAALVVVVLYFVCCSILFLGVKEQPDPLSPASGPGLSYLAGL 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 286 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 365
Cdd:cd17392 236 KLVTGHPPYLKLVIGFLFSSLAFQMEQGNFVLFCTHAAGLGDHFQHLVLAILVSATLSIPLWQWVLQRRGKKTTSFIGIS 315

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 366 SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17392 316 AMVPFLILLALVPSNLPVAYVVAVVSGVSLAALFLLPWSMLPDVVDDFQLKNPHCPGLEPIFYSCYVFFTKLGGGLSLGI 395

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|.
gi 42713696 446 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17392 396 STLSLHFSGYKAGACKQPEEVVVTLKVLIAPVPICLILAGLVLFYVYPINE 446
MFS_MFSD2B cd17452
Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily ...
 46-496 0e+00

Major facilitator superfamily domain-containing protein 2B; Major facilitator superfamily domain-containing protein 2B (MFSD2B) is closely related to MFSD2A, and their conserved genomic structure suggests that they are derived from the duplication of an ancestral gene. Variations of chromosome 2 gene expressions among patients with lung cancer or non-cancer identified MFSD2B as a potential risk or protect factor in the prognosis of lung adenocarcinoma. MFSD2B belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341010  Cd Length: 416  Bit Score: 545.18  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPL 125
Cdd:cd17452   1 KLCYAIGGAPNQVAGSATAFFLQIYLLDIAQITPFQASLVLFVGKAWGAATDPVVGFFISKSKWTKIGRLMPWMLGCTPF 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIV 205
Cdd:cd17452  81 IVVSYFFLWFVPPFTTGRFLWYLTFYCLFQALATCFHVPYSALTMFLSTDQRERDSATAYRMTVEVLGTLVGAAVQGQIV 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 206 GQAdtpcfqdlnsstvasqsanhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGL 285
Cdd:cd17452 161 ASA---------------------------------YMIAAGVIGGLYLLCISVLFLGVKERDDPYAPKSGKAIPFFKGL 207

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 286 RLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAvYVGIS 365
Cdd:cd17452 208 SLTMRHGPYLKLTASFLFISAAVQLEQSNFVLFCTHAVDLHDHFQNLVLTILVSAVVSIPFWQWFLQRFGKKAA-ACGIS 286

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 366 SAVPFLILVALMeSNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17452 287 WMIPFAIMLVTI-PNLIVAYVVAFVSGLSIAASLLLPWSMLPDVVDDFRLQNPHGKGLETIFYSSYVFFTKLSAGIALGI 365

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|.
gi 42713696 446 STLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17452 366 STLSLEFAGYETGACKQPESVVLTLKLLIGAAPTCMILIGLCILMFYPITE 416
MFS_MelB_like cd17332
Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major ...
 46-496 1.31e-102

Salmonella enterica Na+/melibiose symporter MelB and similar transporters of the Major Facilitator Superfamily; This family is composed of Salmonella enterica Na+/melibiose symporter MelB, Major Facilitator Superfamily domain-containing proteins, MFSD2 and MFSD12, and other sugar transporters. MelB catalyzes the electrogenic symport of galactosides with Na+, Li+ or H+. The MFSD2 subfamily is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is more commonly called sodium-dependent lysophosphatidylcholine symporter 1 (NLS1). It is an LPC symporter that plays an essential role for blood-brain barrier formation and function. Inactivating mutations in MFSD2A cause a lethal microcephaly syndrome. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. MelB-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340890 [Multi-domain]  Cd Length: 424  Bit Score: 314.93  E-value: 1.31e-102
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGRLMPWIIFSTPL 125
Cdd:cd17332   1 KIGYGLGDFGNNLIFQIVSTYLLFFYTDVLGLSAAAAGTIFLVARIIDAINDPLMGYLIDRTR-SRWGRFRPWLLWGAIP 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 126 AVIAYFLIWFVPDFPH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQI 204
Cdd:cd17332  80 LALLFVLLFTTPDGSGtGKLIYALITYILLDLLYTLVNIPYTALIPELTDDPEERTSLTSWRMFFATIGGLLVTVLPPPL 159

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 205 VGQADTPCfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPiAYFRG 284
Cdd:cd17332 160 VAYFGGGN-------------------------ASRGYFLTALIIGIIGIILLLICFFGTRERVVPPEEEKSKL-PLLKS 213

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 285 LRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGI 364
Cdd:cd17332 214 LKALLKNRPFLILLLAYLLYFLAFNIVNTVLVYYFKYVLGGRAELVLLLLLILSGALLALLPWPPLKKRFGKKKAFFIGL 293

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 365 SSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKFASGVSL 443
Cdd:cd17332 294 LLAILGLLLLFFLPpGNLVLILVLAVLAGIGYGGANLLPWAMLADVIDYGELK--TGKRREGIFYSVMTFFRKLGLALAG 371

                       410       420       430       440       450
                ....*....|....*....|....*....|....*....|....*....|...
gi 42713696 444 GISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:cd17332 372 ALVGLILSLAGYVANAAAQSASALNGIRLLIAVLPAVLLLLALILMSFYPLDK 424
MFS_2 pfam13347
MFS/sugar transport protein; This family is part of the major facilitator superfamily of ...
 48-496 1.25e-96

MFS/sugar transport protein; This family is part of the major facilitator superfamily of membrane transport proteins.


Pssm-ID: 433134 [Multi-domain]  Cd Length: 427  Bit Score: 299.61  E-value: 1.25e-96
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696    48 CYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTcLGRLMPWIIFSTPLAV 127
Cdd:pfam13347   1 GYGSGALAAGIKYAGLATYLLYFYTDVLGLSAAAVGLVLLVARLVDAFTDPIVGHIIDRTRTR-WGRRRPWLLLSAIILA 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   128 IAYFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVG 206
Cdd:pfam13347  80 VSFILLFTPPELgRAPLFIWLLATYILLRIAYTFFEIPYWSLGPELTRDYDERTSLTSYRSFFSVGGGLLAAALAFPLVL 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   207 qadtpcfqdlnsstvasqsanhthGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVRE---QREPYEAQQSEPIAYFR 283
Cdd:pfam13347 160 ------------------------ILGGTGLERKGYRIFALIGAVLMLLGVIITAAGTKErvsMRSKEDTGQKEAGSLLD 215

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   284 GLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVG 363
Cdd:pfam13347 216 MLKEVFRNRAFLILLASFLLAALAMGVLNGLLLYYFRYVLGNGFAASAFPLVFTIGALLGIPLWPPLAKRIGKKNTYILG 295

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   364 ISSAVPFLILVALM-ESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEpiFFSFYVFFTKFASGVS 442
Cdd:pfam13347 296 ALITIAGFALALLLgPNNTLLFLVLYIIIGFGYGSSFFLPWSMLADVVDYGELRTGKRREGT--FFAMWSFISKLATGVG 373

                         410       420       430       440       450
                  ....*....|....*....|....*....|....*....|....*....|....
gi 42713696   443 LGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDE 496
Cdd:pfam13347 374 LGVSGLLLSAFGYNAGDSVQSPQAVTAIRLLYAVLPAVLFLVALLLLYFYPLDR 427
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
41-512 5.22e-77

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 249.43  E-value: 5.22e-77
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  41 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWII 120
Cdd:COG2211   7 LSLKEKLAYGLGDLGLNLAFGLLSAYLLYFYTDVLGLSAALVGLILLVARLWDAITDPLIGA-LSDRTRTRWGRRRPWIL 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 121 FSTPLAVIAYFLIWFVPDFPHGQTYWYLL-FYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTA 199
Cdd:COG2211  86 IGAIPLALSFVLLFTAPDLSPTGKLIYALvTYLLLGLAYTLVNIPYSALGAELTPDYEERTRLSSWRFAFAGLGGLLASV 165

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 200 IQGQIVGQADTpcfqdlnsstvasqsanhthgttshrETQKAYLLAAGVIVCIYIICAVILILGVREQREPyeaqQSEPI 279
Cdd:COG2211 166 LPPPLVAAFGG--------------------------DAALGYRLTALIFAVLGLLAFLLTFFGTKERPVP----EEEKV 215

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 280 AYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKT 358
Cdd:COG2211 216 SLKESLKALLKNRPFLLLLLAYLLFFLALALVAALLLYYFKYVLGLsAALVGLLLALYFLAALLGAPLWPRLAKRFGKKK 295

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 359 AVYVGIS-SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKqpHFHGTEPIFFSFYVFFTKF 437
Cdd:COG2211 296 AFIIGLLlAALGLLLLFFLGPGNLWLLLVLAALAGIGLGAILVLPWAMLADVVDYDEWK--TGRRREGLYFGIFTFAIKL 373

                       410       420       430       440       450       460       470
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 42713696 438 ASGVSLGISTLSLDFAGYQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDE 512
Cdd:COG2211 374 GQALAGALAGLLLALFGYVA-GAAQSPSALTGIRLLFFLLPAVLLLLAALLLLFYPLTRERHAEIRAELAARRAA 447
gph TIGR00792
sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH): ...
 46-501 5.65e-30

sugar (Glycoside-Pentoside-Hexuronide) transporter; The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family (TC 2.A.2) GPH:cation symporters catalyze uptake of sugars in symport with a monovalent cation (H+ or Na+). Members of this family includes transporters for melibiose, lactose, raffinose, glucuronides, pentosides and isoprimeverose. Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria, and the lactose permease of Streptococcus thermophilus) have been isolated in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. All of these proteins possess twelve putative transmembrane a-helical spanners. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]


Pssm-ID: 273270  Cd Length: 437  Bit Score: 121.97  E-value: 5.65e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696    46 KLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPWI-IFS 122
Cdd:TIGR00792   1 KLSYGFGDFGNDFIFAIVSTYLLFFYTDVLGLSAAFVGTLFLVARILDAITDPIMGNIVdrTRTRW---GKFRPWLlIGA 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   123 TPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMtvevlgtvLGTAIQ 201
Cdd:TIGR00792  78 IPFSIVL-VLLFTTPDFsGTGKLVYAYITYILLGLFYSFVNIPYWSLVPAITLDPRERESLSTFRR--------FGATLG 148

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   202 GQIVGQADTPcfqdlnsSTVASQSANHTHGttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAY 281
Cdd:TIGR00792 149 GLLVAVIVLP-------LVSYFGGGDDKFG----------WFMFALVLALIGVVSLIICFFGTKERYSEIPKNIEKKLSL 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   282 FRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVY 361
Cdd:TIGR00792 212 KQIFKALFKNDQLLILCLAYLFYNLAFNIKNGVQVYYFTYVLGDPELFSYMGSIAIVAGLIGVLLFPRLVKKFGRKILFA 291

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   362 VGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPhfHGTEPIFFSFYVFFTKFASGV 441
Cdd:TIGR00792 292 GGILLMVLGYLIFFFAGSNLPLILVLIILAGFGQNIVTGLVWALVADTVDYGEWKTG--IRAEGLVYSVRTFVRKLGQAL 369

                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 42713696   442 SLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLF-KMYPIDEERRRQ 501
Cdd:TIGR00792 370 AGFFVGLILGIIGY-VANAAQSPITLNGIKILMFAVPALFLLLAAIIIgRFYKLTEKKHAE 429
PRK09669 PRK09669
putative symporter YagG; Provisional
 40-498 9.26e-18

putative symporter YagG; Provisional


Pssm-ID: 236610  Cd Length: 444  Bit Score: 85.55  E-value: 9.26e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   40 QLSVCNKLCYALGGapyqvTGCAL-----GFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGR 114
Cdd:PRK09669   5 QLTTKDKIGYGLGD-----TACNLvwqtvMLFLAYFYTDVFGLSAAIMGTMFLVVRVLDAVTDPLMGALVDRTR-TRHGQ 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  115 LMPWII-FSTPLAVIAyFLIWFVPDF-PHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVL 192
Cdd:PRK09669  79 FRPYLLwFAIPFGVVC-LLTFYTPDFgATGKIIYACVTYILLSLVYTAINVPYCAMPGAITNDPRERHSLQSWRFALSFI 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  193 GTVLGTAIQGQIV---GQADtpcfqdlnsstvasqsanhthgttshreTQKAYLLAAGVIVCIYIICAVILILGVREQRE 269
Cdd:PRK09669 158 GGLIVSVIALPLVdilGKGD----------------------------EQKGYFYAMMVMGLLGVVLFFCCFFMTKERYT 209

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  270 PYEAQQSepiAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQW 349
Cdd:PRK09669 210 PEVDNSS---SVWKDLKLLLGNSQWRIMFIFNVVLLTAVVTRGGATLYYVNYVLLRPDLATLFLVTGMIAGLFGALLSER 286

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  350 FLTRFGKKTAVYVGISSAVPFLILVALME-SNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfHLKQPHFHGTEPIFF 428
Cdd:PRK09669 287 LLGKFDRVRAFKWTIVAFVILSALIFFIPpSNVWLIFALNILFNFIQNLTTPLQWSMFSDVVD--YEEKRSGRRLDGLVF 364

                        410       420       430       440       450       460       470
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 42713696  429 SFYVFFTKFasGVSLGISTL--SLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEER 498
Cdd:PRK09669 365 STNLFAIKL--GLAIGGAVVgwILAWVDYVGGAAVQSASVLTTINLLFTVIPGVLFAGMAIILNFYKLNDAR 434
PRK10429 PRK10429
melibiose:sodium transporter MelB;
41-528 7.14e-13

melibiose:sodium transporter MelB;


Pssm-ID: 182453  Cd Length: 473  Bit Score: 70.51  E-value: 7.14e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   41 LSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLMPW 118
Cdd:PRK10429   3 ISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGWIVnnTRSRW---GKFKPW 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  119 IIFSTPLAVIAYFLIWFVPDFPHGQTYWYL-LFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLG 197
Cdd:PRK10429  80 ILIGTLANSVVLFLLFSAHLFEGTAQYVFVcVTYILWGMTYTIMDIPFWSLVPTLTLDKREREQLVPYPRFFASLAGFVT 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  198 TAIQGQIVGQadtpcfqdlnsstvasqsanhthgtTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSE 277
Cdd:PRK10429 160 AGFTLPFVNY-------------------------VGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVYSSDNQVSAE 214

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  278 PIAY-FRG-LRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFG 355
Cdd:PRK10429 215 GSHLtLKDiVALIYKNDQLSCLLGMALAYNIASNIINGFAIYYFTYVIGDADLFPYYLSYAGAANLVTLILFPRLVKSLS 294

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  356 KKTaVYVGISSaVPFLILVALMesnLIITYAVAVAAGISVAAAFL-----LPWS----MLPDVID--DFHLKQPhfhgTE 424
Cdd:PRK10429 295 RRI-LWAGASI-FPVLSCGVLL---LMGLAAPHNALLIVIAGILLnigtaLFWVlqviMVADTVDygEYKLGIR----CE 365

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  425 PIFFSFYVFFTKFASGVSLGISTLSLDFAGYqTRGCSQPERVKFTLNMLVTMAPIVLILLGLLL-FKMYPIDEERRRQNK 503
Cdd:PRK10429 366 SIAYSVQTMVVKGGSAFAAFFIGVVLGLIGY-VPNVEQSAQTLLGMQFIMIGLPTLFFMITLVLyFRYYRLNGDFLRRIQ 444

                        490       500
                 ....*....|....*....|....*
gi 42713696  504 KALQALRDEASSSGCSETDSTELAS 528
Cdd:PRK10429 445 IHLLDKYRKVPPEPVHAEIPVGAVS 469
PRK09848 PRK09848
glucuronide transporter; Provisional
 39-513 4.10e-12

glucuronide transporter; Provisional


Pssm-ID: 182109 [Multi-domain]  Cd Length: 448  Bit Score: 68.27  E-value: 4.10e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   39 QQLSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPwTCLGRLMPW 118
Cdd:PRK09848   3 QQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAAAAGTMLLLVRVFDAFADVFAGRVVDSVN-TRWGKFRPF 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  119 IIF-STPLAVIAYFLIWFVPDFPHGQTYWY-LLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRM-----TVEV 191
Cdd:PRK09848  82 LLFgTAPLMIFSVLVFWVPTDWSHSSKVVYaYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGiaaslTFVC 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  192 LGTVLGTAIQgqivgqadtpcfqdlnSSTVASQSANHTHGTtshretqkayLLAAGVIVCIYIICavililgVREQREPY 271
Cdd:PRK09848 162 LAFLIGPSIK----------------NSSPEEMVSVYHFWT----------IVLAIAGMVLYFIC-------FKSTRENV 208

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  272 EAQQSEPiAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATL-TIPIWQWF 350
Cdd:PRK09848 209 VRIVAQP-SLKISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVGTVaSAPLVPGM 287

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  351 LTRFGKKTAVYVGIS-SAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQphfhG--TEPIF 427
Cdd:PRK09848 288 VARIGKKNTFLIGALlGTCGYLLFFWVSVWSLPVALVALAIASIGQGVTMTVMWALEADTVEYGEYLT----GvrIEGLT 363

                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  428 FSFYVFFTKFASGVSLGISTLSLDFAGYQTrGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQ 507
Cdd:PRK09848 364 YSLFSFTRKCGQAIGGSIPAFILGLSGYIA-NQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEID 442


                 ....*.
gi 42713696  508 ALRDEA 513
Cdd:PRK09848 443 NRKKVQ 448
PRK11462 PRK11462
putative transporter; Provisional
 39-205 2.56e-10

putative transporter; Provisional


Pssm-ID: 183145  Cd Length: 460  Bit Score: 62.65  E-value: 2.56e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696   39 QQLSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCI--SKSPWtclGRLM 116
Cdd:PRK11462   4 EVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPAGFVGTMFLVARALDAISDPCMGLLAdrTRSRW---GKFR 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  117 PWIIF-STPLAVIAyFLIWFVPDFPH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGT 194
Cdd:PRK11462  81 PWVLFgALPFGIVC-VLAYSTPDLSMnGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGG 159

                        170
                 ....*....|.
gi 42713696  195 VLGTAIQGQIV 205
Cdd:PRK11462 160 MLSTVLMMPLV 170
MFS_MFSD12 cd17491
Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily ...
 66-442 2.08e-07

Major facilitator superfamily domain-containing protein 12; Major facilitator superfamily domain-containing protein 12 (MFSD12) protein subfamily includes a group of uncharacterized proteins similar to human MFSD2. MFSD2 is composed of two vertebrate members, MFSD2A and MFSD2B. MFSD2A is an LPC symporter that plays an essential role for blood-brain barrier formation and function. MFSD2B is a potential risk or protect factor in the prognosis of lung adenocarcinoma. The MFSD12 subfamily belongs to the Salmonella enterica Na+/melibiose symporter like (MelB-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341044  Cd Length: 438  Bit Score: 53.42  E-value: 2.08e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  66 FLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPW-TCLGRLMPWIIFSTPLAVIAYFLIW-----FVPDF 139
Cdd:cd17491  21 YLLVYFHLVLGFSSALAGILLLIGQVADAISTPLVGYESDRTNGcGKYGRRKSWHLIGTICVLLSFPFIFnpclgCSDST 100

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 140 PH-GQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYR--MTVEVLGTVLGTA--IQGQIVGQADTPcfq 214
Cdd:cd17491 101 SEwAKLVYYGPFIIIFQFGWAAVQISHLSLIPELTSDEHERVELTALRyaFTVIANITVYLIAwlLLQQSSGDSVNP--- 177

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 215 dlNSSTVASQSANhthgttshretqkayllAAGVIVCIYIICAVILILGVREQREP--YEAQQSEPIAYFRGLRLVMSHG 292
Cdd:cd17491 178 --TSAQDLPIFRN-----------------LSLIVVGIGFLFSLLFHLGTKEPRPPtdLSTTTNEGEEVTAPEEPRLTWK 238

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 293 PYIKL----ITGFLFTS--LAFMLVEGNFVLFCTYTLGFRNEFQNLL-LAIMLSATLTIPIWQWFLTRFGKKTAVYVGIS 365
Cdd:cd17491 239 EWLREpqfyQVALLYMCtrLIVNLSQVYIPLYLTETLQLPKESIAIVpLVMYVSGFLTSLVMKPINKKIGRKITYLLGLL 318

                       330       340       350       360       370       380       390
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 42713696 366 SAVPFLILVAL--MESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDdfhlkqpHFHGTEPIFFSFYVFFTKFASGVS 442
Cdd:cd17491 319 FVLGFCIWVWFqsGSSRTYEIYGAAVLLGVGSATLLVTSLSMTADLIG-------TNTESGAFVYGAMSFTDKLSNGIA 390
MFS_SLC45_SUC cd17313
Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily ...
 47-445 5.82e-06

Solute carrier family 45 and similar sugar transporters of the Major Facilitator Superfamily of transporters; This group includes the solute carrier 45 (SLC45) family as well as plant sucrose transporters (SUCs or SUTs) and similar proteins such as Schizosaccharomyces pombe general alpha-glucoside permease. the SLC45 family is composed of four (A1-A4) vertebrate proteins as well as related insect proteins such as Drosophila sucrose transporter SCRT or Slc45-1. Members of this group transport sucrose and other sugars like maltose into the cell, with the concomitant uptake of protons (symport system). Plant sucrose transporters are crucial to carbon partitioning, playing a key role in phloem loading/unloading. They play a key role in loading and unloading of sucrose into the phloem and as a result, they control sucrose distribution throughout the whole plant and drive the osmotic flow system in the phloem. They also play a role in the exchange of sucrose between beneficial symbionts (mycorrhiza and Rhizobium) as well as pathogens such as nematodes and parasitic fungi. There are nine sucrose transporter genes in Arabidopsis and five in rice. Vertebrate SLC45 family proteins have been implicated in the regulation of glucose homoeostasis in the brain (SLC45A1), with skin and hair pigmentation (SLC45A2), and with prostate cancer and myelination (SLC45A3). Mutations in SLC45A2, also called MATP (membrane-associated transporter protein) or melanoma antigen AIM1, cause oculocutaneous albinism type 4 (OCA4), an autosomal recessive disorder of melanin biosynthesis that results in congenital hypopigmentation of ocular and cutaneous tissues. The SLC45 family and related sugar transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340871 [Multi-domain]  Cd Length: 421  Bit Score: 48.78  E-value: 5.82e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  47 LCYALGGApyqvtgcalgfFLQIYLLDVAqVGPFSASIILFVGRAWDAITDPLVGLcISKSPWTCLGRLMPWIIFSTPLA 126
Cdd:cd17313  13 FGWALENS-----------YVPPILQTLG-LSHALIGFVWTLGPILGLFVQPLVGS-LSDRCNSRIGRRRPFILVGAPLA 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 127 VIAYFLIWFVPDFPHG---QTYW-----YLLFYCLFETMVTCFHVPYSALtMFISTEQTERDSATAYRMTVEVLGTVLGT 198
Cdd:cd17313  80 ALGLILIPNAADIGLAlgdGPRTwalvlFVLGIVLLDFAMNVCQGPVRAL-LPDLVPPEQRSKANGIINFMGGLGGVLGY 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 199 AIQGQivgqadtpcfqdLNSSTVAsqsanhthGTTSHRETQKAYLLAAGVIVciyiICAVILILGVREQR----EPYEAQ 274
Cdd:cd17313 159 LLGAI------------LWDHNWF--------GFALGGNLKVPFYIGAIILL----VCVLVTLFFVKEPPlspeAGEHSE 214

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 275 QSEPIAYFRGLRLVMSHGP--YIKLITGFLFTSLAFMLVEGNFVLFCT-----------YTLGFRNEFQNLLLAImLSAT 341
Cdd:cd17313 215 NPSLGEVLKSLLKLLKIMPrsLLRLLLVIFFWWIAFFPFELFFTDYMGeevyhgtsaasSHYGAGVRMGAWGLLI-FSLA 293

                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 342 LTI---PIwQWFLTRFGKKtAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQp 418
Cdd:cd17313 294 FLIfslPI-GKLGKKIGRK-KVYLIGLVLFAVGMALMALVHNVTVALVLFALGGIGWATININPYPLVSDYHSESGRGQ- 370

                       410       420
                ....*....|....*....|....*..
gi 42713696 419 hfhGTEPIFFSFYVFFTKFASGVSLGI 445
Cdd:cd17313 371 ---GTDTGLLNLAISLAQILVSLLAGP 394
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 242-399 2.08e-05

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 46.80  E-value: 2.08e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 242 YLLAAGVIVCIYIICAVILILG-------VREQREPYEAQQSEPIAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGN 314
Cdd:cd17325 142 LLADALGYRAPFLVCAALALLAlvlalllLPEPRPPPNKRAVSAARLRSGLRLLLRDRRLLALFLAIFVLAFAFGALEPF 221

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 315 FVLFCTYTLGFrNEFQN--LLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMeSNLIITYAVAVAAG 392
Cdd:cd17325 222 LPLYAAELGGL-SPAQIglLFGAQGLASALSQPPAGKLSDRIGRKPLILIGLLLSAVALLLLPLA-TSFWLLLLLLALLG 299


                ....*..
gi 42713696 393 ISVAAAF 399
Cdd:cd17325 300 LGLGLVF 306
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
245-409 1.48e-04

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 44.10  E-value: 1.48e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 245 AAGVIVCIYIICAVILILGVREqrEPYEAQQSEPIAyFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLG 324
Cdd:COG2223 161 AFLILGILLLVVAVLAWLFLRD--PPAGAAAAAKAS-LRDQLEALRDPRFWLLSLLYFGTFGSFIGFSSWLPPYLVDQFG 237

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 325 FRNEFQNLLLAIM-LSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPW 403
Cdd:COG2223 238 LSAATAGLLAALFaLLGALGRPLGGWLSDRIGGRRVLLIVFALMALGLLLLALALGSLWLFLVLFLLLGLALGGGNGAVF 317


                ....*.
gi 42713696 404 SMLPDV 409
Cdd:COG2223 318 ALVPDI 323
MFS_FsR cd17478
Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters; ...
 65-399 4.10e-04

Fosmidomycin resistance protein of the Major Facilitator Superfamily of transporters; Fosmidomycin resistance protein (FsR) confers resistance against fosmidomycin. It shows sequence similarity with the bacterial drug-export proteins that mediate resistance to tetracycline and chloramphenicol. This FsR family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341031 [Multi-domain]  Cd Length: 365  Bit Score: 42.54  E-value: 4.10e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  65 FFLQIYLLDVAQVGpfsasIILFVGRAWDAITDPLVGLCISKSPWtclGRLMPWIIFSTPLAVIayfLIWFVPDFphgqt 144
Cdd:cd17478  21 LLIAKLGLSYAQAG-----LLVSVFSITSSLLQPLFGYLADRKPR---RYLLPLGPLLTGLGLS---LLGFAPNY----- 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 145 YWYLLFYCLFETMVTCFHVPYSALTMFISTEQteRDSATAYRMTVEVLGTVLGTAIQGQIVgqadtpcfqdlnsstvasq 224
Cdd:cd17478  85 WLLLLLVALAGIGSAAFHPEGARLASQAAGGR--RGLAMSIFSVGGNLGFALGPLIAAALV------------------- 143

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 225 sanhthgttshretQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGLRLVMshGPYIKLITGFLFT 304
Cdd:cd17478 144 --------------ALFGGLGLLILLIIPGLAVALLLLRLRPLKKPEAKKAAAKSSARSTLKGVW--RALLLLLIIVFLR 207

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 305 SLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALmeSNLIIT 384
Cdd:cd17478 208 SWVLSSLTTFLPFYLVQRGYSLVFAGLLLSLFLFAGAIGTLIGGPLSDRFGRRRVIIASLLLAAPFLLLFLL--TPGIWS 285

                       330
                ....*....|....*
gi 42713696 385 YAVAVAAGISVAAAF 399
Cdd:cd17478 286 YILLVLAGFLLFASF 300
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
119-409 6.48e-04

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 41.88  E-value: 6.48e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 119 IIFSTPLAVIAYFLIWFVPDFPhgqtyWYLLFYCLFETMVTCFHVPYSALTMFISTEQtERDSATAYRMTVEVLGTVLGT 198
Cdd:COG2814  78 LLLGLLLFALGSLLCALAPSLW-----LLLAARFLQGLGAGALFPAALALIADLVPPE-RRGRALGLLGAGLGLGPALGP 151

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 199 AIQGQIVGQADtpcfqdlnsstvasqsanhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSep 278
Cdd:COG2814 152 LLGGLLADLFG--------------------------------WRWVFLVNAVLALLALLLLLRLLPESRPAARARLR-- 197

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 279 iayfRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGF-RNEFQNLLLAIMLSATLTIPIWQWFLTRFGKK 357
Cdd:COG2814 198 ----GSLRELLRRPRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLsASAAGLLLALFGLGGVLGALLAGRLADRFGRR 273

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|..
gi 42713696 358 TAVYVGISSAVPFLILVALMeSNLIITYAVAVAAGISVAAAFLLPWSMLPDV 409
Cdd:COG2814 274 RLLLIGLLLLALGLLLLALA-GSLWLLLLALFLLGFGFGLLFPLLQALVAEL 324
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 66-405 1.81e-03

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 40.87  E-value: 1.81e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696  66 FLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKspwtcLGRLmPWIIFSTPLAVIAYFLIWFVPDFphgqtY 145
Cdd:cd06174  17 PLLPALLQSFGLSASQLGLLFALFSLGYALLQPLAGLLADR-----FGRR-PVLLLGLLLFALGALLFAFAPSF-----W 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 146 WYLLFYCLFETMVTCFHVPYSALTMFiSTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVGQADTPcfqdlnsstvasqs 225
Cdd:cd06174  86 LLLLGRFLLGLGSGLIDPAVLALIAD-LFPERERGRALGLLQAFGSVGGILGPLLGGILASSLGFG-------------- 150

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 226 anhthgttshretqkaYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIA--YFRGLRLVMSHGPYIKLITGFLF 303
Cdd:cd06174 151 ----------------WRAVFLIAAALALLAAILLLLVVPDPPESARAKNEEASSksVLKLLKRVLKNPGLWLLLLAIFL 214

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 304 TSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAI-MLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLI 382
Cdd:cd06174 215 VNLAYYSFSTLLPLFLLDLGGLSVAVAGLLLSLfGLAGALGSLLLGLLSDRLIGRKPLLLIGLLLMALGLALLLLAPSLL 294

                       330       340
                ....*....|....*....|...
gi 42713696 383 ITYAVAVAAGISVAAAFLLPWSM 405
Cdd:cd06174 295 LLLLLLLLLGFGLGGLLPLSFAL 317
BtlA COG2270
MFS-type transporter involved in bile tolerance, Atg22 family [General function prediction ...
 113-439 3.98e-03

MFS-type transporter involved in bile tolerance, Atg22 family [General function prediction only];


Pssm-ID: 441871 [Multi-domain]  Cd Length: 424  Bit Score: 39.75  E-value: 3.98e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 113 GRLMPWIIFSTPLAVIAYFLIWFVPDfphGQTYWYLLFYCL----FETMVtcfhVPYSALTMFISTEQtERD--SATAYR 186
Cdd:COG2270  80 GRKKPFLAFFTLLGVLATAALFFVAP---GGLWLGLLLFVLanigFEGSL----VFYNAFLPDIATPE-EIGrvSGLGWA 151

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 187 M----TVEVLGTVLGTAIQGQIVGQADTpcfqdlnsstvasqsanhthgttsHRETQKAYLLAAGVivciYIICAVILIL 262
Cdd:COG2270 152 LgylgGVLLLLINLALFLKPEAFGLPDA------------------------GEAVRISFLLTALW----WLLFALPLFL 203

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 263 GVREQREPYEAQQSEPIAY-FRGLRLVMSHGPYIKLITGFLftsLAFML----VEGNFVLFCTY---TLGFrnEFQNLLL 334
Cdd:COG2270 204 FLPERPRPGPAPPRGAVRAgFRELRRTLRELRRYRDLLRFL---LAYFFyrdgVQTVIAFAGIYaagVLGF--STTELIV 278

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42713696 335 AIMLSATLTIP---IWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIItYAVAVAAGISVAAAFLLPWSMLPDVId 411
Cdd:COG2270 279 FGLLVQIVAALgalLFGRLDDRIGPKRVILVSLVIWIVVCLAAYFLTTALAF-WILGLLIGLVMGGIQALSRSLLARLI- 356

                       330       340
                ....*....|....*....|....*...
gi 42713696 412 dfhlkqPHFHGTEpiFFSFYVFFTKFAS 439
Cdd:COG2270 357 ------PPGREAE--FFGLYALSGKAAS 376
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH