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Conserved domains on  [gi|30698726|ref|NP_177212|]
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NEP-interacting protein, putative (DUF239) [Arabidopsis thaliana]

Protein Classification

neprosin family protein( domain architecture ID 10627288)

neprosin family protein similar to the proline-cleaving enzyme neprosin

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Neprosin pfam03080
Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. ...
 246-458 1.22e-121

Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. The neprosin domain corresponds to the mature peptidase. It is not known if other proteins with this domain are peptidases.


:

Pssm-ID: 460797  Cd Length: 211  Bit Score: 353.47  E-value: 1.22e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   246 QYEFSLSQIWVIAGSFthDLNTIEAGWQISPELYGDTYPRFFTYWTSDAYRTTGCYNLLCSGFVQTNRRIAIGAAISPRS 325
Cdd:pfam03080   1 PDQFSLAQIWISSGPG--GLNSIEAGWQVNPSLYGDSRTRLFTYWTADGYQKTGCYNLLCPGFVQVSSDIPLGGAISPVS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   326 SYKGGQFDISLLIWKDPKHGHWWLQFGSGALVGYWPAFLFTHLKQHG-SMVQFGGEIVNnrPGGSHTTTQMGSGHFAGEG 404
Cdd:pfam03080  79 VYGGKQYEITLSIFKDPKTGNWWLYYGGDEVIGYWPASLFTHLLAGSaNLVEWGGEVYS--PTGSHTSPPMGSGHFPSEG 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 30698726   405 FGKASYFRNLQIVDWDNTLIPASNLKILADHPNCYDI-RGGTNRVWGNYFYYGGP 458
Cdd:pfam03080 157 FGKAAYFRNIKIVDDSNGLPPPQDLETLADSPKCYNViIFGYSGDWGSYFYYGGP 211
Neprosin_AP pfam14365
Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid ...
 117-223 1.18e-47

Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. This domain corresponds to the presumed activation peptide.


:

Pssm-ID: 464153  Cd Length: 119  Bit Score: 159.79  E-value: 1.18e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   117 TIQSSDGDKIDCVSTHQQPAFDHPLLQGQKP-LDPPEIPKGYSEDDGSYENS------QLWSLSGESCPEGTIPIRRTTE 189
Cdd:pfam14365   1 SIKSPDGDIIDCVDIHKQPAFDHPLLKNHTIqMKPPERPEGTLLESKNDGSSkektawQLWHSNGISCPEGTVPIRRTTE 80

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 30698726   190 QDMLRASSVQRFGRKIRR-----VKRDSTNNGHEHAVGY 223
Cdd:pfam14365  81 DDLLRASSLKRFGKKPRRsrpksDRPDTSGNGHEHAVAY 119
 
Name Accession Description Interval E-value
Neprosin pfam03080
Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. ...
 246-458 1.22e-121

Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. The neprosin domain corresponds to the mature peptidase. It is not known if other proteins with this domain are peptidases.


Pssm-ID: 460797  Cd Length: 211  Bit Score: 353.47  E-value: 1.22e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   246 QYEFSLSQIWVIAGSFthDLNTIEAGWQISPELYGDTYPRFFTYWTSDAYRTTGCYNLLCSGFVQTNRRIAIGAAISPRS 325
Cdd:pfam03080   1 PDQFSLAQIWISSGPG--GLNSIEAGWQVNPSLYGDSRTRLFTYWTADGYQKTGCYNLLCPGFVQVSSDIPLGGAISPVS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   326 SYKGGQFDISLLIWKDPKHGHWWLQFGSGALVGYWPAFLFTHLKQHG-SMVQFGGEIVNnrPGGSHTTTQMGSGHFAGEG 404
Cdd:pfam03080  79 VYGGKQYEITLSIFKDPKTGNWWLYYGGDEVIGYWPASLFTHLLAGSaNLVEWGGEVYS--PTGSHTSPPMGSGHFPSEG 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 30698726   405 FGKASYFRNLQIVDWDNTLIPASNLKILADHPNCYDI-RGGTNRVWGNYFYYGGP 458
Cdd:pfam03080 157 FGKAAYFRNIKIVDDSNGLPPPQDLETLADSPKCYNViIFGYSGDWGSYFYYGGP 211
Neprosin_AP pfam14365
Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid ...
 117-223 1.18e-47

Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. This domain corresponds to the presumed activation peptide.


Pssm-ID: 464153  Cd Length: 119  Bit Score: 159.79  E-value: 1.18e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   117 TIQSSDGDKIDCVSTHQQPAFDHPLLQGQKP-LDPPEIPKGYSEDDGSYENS------QLWSLSGESCPEGTIPIRRTTE 189
Cdd:pfam14365   1 SIKSPDGDIIDCVDIHKQPAFDHPLLKNHTIqMKPPERPEGTLLESKNDGSSkektawQLWHSNGISCPEGTVPIRRTTE 80

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 30698726   190 QDMLRASSVQRFGRKIRR-----VKRDSTNNGHEHAVGY 223
Cdd:pfam14365  81 DDLLRASSLKRFGKKPRRsrpksDRPDTSGNGHEHAVAY 119
 
Name Accession Description Interval E-value
Neprosin pfam03080
Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. ...
 246-458 1.22e-121

Neprosin; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. The neprosin domain corresponds to the mature peptidase. It is not known if other proteins with this domain are peptidases.


Pssm-ID: 460797  Cd Length: 211  Bit Score: 353.47  E-value: 1.22e-121
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   246 QYEFSLSQIWVIAGSFthDLNTIEAGWQISPELYGDTYPRFFTYWTSDAYRTTGCYNLLCSGFVQTNRRIAIGAAISPRS 325
Cdd:pfam03080   1 PDQFSLAQIWISSGPG--GLNSIEAGWQVNPSLYGDSRTRLFTYWTADGYQKTGCYNLLCPGFVQVSSDIPLGGAISPVS 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   326 SYKGGQFDISLLIWKDPKHGHWWLQFGSGALVGYWPAFLFTHLKQHG-SMVQFGGEIVNnrPGGSHTTTQMGSGHFAGEG 404
Cdd:pfam03080  79 VYGGKQYEITLSIFKDPKTGNWWLYYGGDEVIGYWPASLFTHLLAGSaNLVEWGGEVYS--PTGSHTSPPMGSGHFPSEG 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 30698726   405 FGKASYFRNLQIVDWDNTLIPASNLKILADHPNCYDI-RGGTNRVWGNYFYYGGP 458
Cdd:pfam03080 157 FGKAAYFRNIKIVDDSNGLPPPQDLETLADSPKCYNViIFGYSGDWGSYFYYGGP 211
Neprosin_AP pfam14365
Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid ...
 117-223 1.18e-47

Neprosin activation peptide; Pitcher plants are insectivorous and secrete a digestive fluid into the pitcher. This fluid contains a mixture of enzymes including peptidases. One of these is neprosin, characterized from the pitcher plant Nepenthes ventrata. This peptidase is of unknown catalytic type and is unaffected by standard peptidase inhibitors. Unusually, activity is directed towards prolyl bonds, but unlike most peptidase that cleave after proline, there is no restriction on sequence length or position of the proline residue. The peptidase is secreted and is presumed to possess an N-terminal activation peptide. This domain corresponds to the presumed activation peptide.


Pssm-ID: 464153  Cd Length: 119  Bit Score: 159.79  E-value: 1.18e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30698726   117 TIQSSDGDKIDCVSTHQQPAFDHPLLQGQKP-LDPPEIPKGYSEDDGSYENS------QLWSLSGESCPEGTIPIRRTTE 189
Cdd:pfam14365   1 SIKSPDGDIIDCVDIHKQPAFDHPLLKNHTIqMKPPERPEGTLLESKNDGSSkektawQLWHSNGISCPEGTVPIRRTTE 80

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 30698726   190 QDMLRASSVQRFGRKIRR-----VKRDSTNNGHEHAVGY 223
Cdd:pfam14365  81 DDLLRASSLKRFGKKPRRsrpksDRPDTSGNGHEHAVAY 119
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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