forkhead associated (FHA) domain found in sarcolemmal membrane-associated protein (SLMAP) and similar proteins; SLMAP, also called sarcolemmal-associated protein (SLAP), is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. It is a cardiac membrane protein that plays an important role in E-C coupling and the adrenergic response of the heart. Overexpression of the SLMAP gene has been associated with diabetes and endothelial dysfunction of macro- and micro-blood vessels. SLMAP contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   2 SAVITLTPLNES--FQTKKLVISPstIYKIGRHTNKSTsPSPSNLFFNSKVLSRQHAELWLDKdtLSVYIRDVKSSNGTF 79
Cdd:cd22679   1 SALAILTPRPNShpFQERHIVLDE--PVKIGRSVARAR-PAANNAIFDCKVLSRNHALLWYDD--GKFYLQDTKSSNGTF 75

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|.
gi 19112554  80 VNETRLSPENKPSAPCKLNSGDIVDFGVDIYNEDE-IVHQKVSAQVRIVVR 129
Cdd:cd22679  76 VNNQRLSKGSEESEPRELHSGDIVQFGVDVVENSRkVTHGCIVATVTLFLP 126

forkhead associated (FHA) domain found in sarcolemmal membrane-associated protein (SLMAP) and similar proteins; SLMAP, also called sarcolemmal-associated protein (SLAP), is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. It is a cardiac membrane protein that plays an important role in E-C coupling and the adrenergic response of the heart. Overexpression of the SLMAP gene has been associated with diabetes and endothelial dysfunction of macro- and micro-blood vessels. SLMAP contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   2 SAVITLTPLNES--FQTKKLVISPstIYKIGRHTNKSTsPSPSNLFFNSKVLSRQHAELWLDKdtLSVYIRDVKSSNGTF 79
Cdd:cd22679   1 SALAILTPRPNShpFQERHIVLDE--PVKIGRSVARAR-PAANNAIFDCKVLSRNHALLWYDD--GKFYLQDTKSSNGTF 75

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|.
gi 19112554  80 VNETRLSPENKPSAPCKLNSGDIVDFGVDIYNEDE-IVHQKVSAQVRIVVR 129
Cdd:cd22679  76 VNNQRLSKGSEESEPRELHSGDIVQFGVDVVENSRkVTHGCIVATVTLFLP 126

Forkhead associated (FHA) domain, binds pSer, pThr, pTyr [Signal transduction mechanisms];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  27 YKIGRHTNkstspspSNLFFNSKVLSRQHAELWLDKDTlsVYIRDVKSSNGTFVNETRLspenkpSAPCKLNSGDIVDFG 106
Cdd:COG1716  23 LTIGRAPD-------NDIVLDDPTVSRRHARIRRDGGG--WVLEDLGSTNGTFVNGQRV------TEPAPLRDGDVIRLG 87

forkhead associated (FHA) domain found in sarcolemmal membrane-associated protein (SLMAP) and similar proteins; SLMAP, also called sarcolemmal-associated protein (SLAP), is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. It is a cardiac membrane protein that plays an important role in E-C coupling and the adrenergic response of the heart. Overexpression of the SLMAP gene has been associated with diabetes and endothelial dysfunction of macro- and micro-blood vessels. SLMAP contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   2 SAVITLTPLNES--FQTKKLVISPstIYKIGRHTNKSTsPSPSNLFFNSKVLSRQHAELWLDKdtLSVYIRDVKSSNGTF 79
Cdd:cd22679   1 SALAILTPRPNShpFQERHIVLDE--PVKIGRSVARAR-PAANNAIFDCKVLSRNHALLWYDD--GKFYLQDTKSSNGTF 75

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|.
gi 19112554  80 VNETRLSPENKPSAPCKLNSGDIVDFGVDIYNEDE-IVHQKVSAQVRIVVR 129
Cdd:cd22679  76 VNNQRLSKGSEESEPRELHSGDIVQFGVDVVENSRkVTHGCIVATVTLFLP 126

forkhead associated (FHA) domain found in Saccharomyces cerevisiae defective in mitotic arrest protein 1 (DMA1), 2 (DMA2) and similar proteins; DMA1 (also known as checkpoint forkhead associated with RING domains-containing protein 1, or CHF1) and DMA2 (also known as checkpoint forkhead associated with RING domains-containing protein 2, or CHF2) are E3 ubiquitin-protein ligases which function in cell cycle retarding in conjunction with the UBC4 and UBC13/MMS2 complex, two E2 ubiquitin conjugating enzymes. They are involved in nutritional control of the cell cycle and required for proper spindle positioning, likely regulating septin ring deposition at the bud neck. DMA1 targets the degradation of G1 cyclin PCL1. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  24 STIYkIGRHTNKSTSPSPSNLFFNSKVLSRQHAELWLDKDTLsvYIRDVKSSNGTFVNETRLSPENKPSAPCKLNSGDIV 103
Cdd:cd22692  37 SQIH-IGRYTERVRQAIYHPVVFKSKVVSRTHGCIKVDEGNW--YIKDVKSSSGTFLNHQRLSPASRTSKPYPLRDGDIL 113

                        90       100
                ....*....|....*....|...
gi 19112554 104 DFGVDIYNEDEIVHQKVSAQVRI 126
Cdd:cd22692 114 QLGMDYRGGTEEIYRCVRMRVEL 136

FHA domain; The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 19112554    27 YKIGRHtnkstspSPSNLFFNSKVLSRQHAELWLDKDtLSVYIRDVKSSNGTFVNETRLSPEnkpsaPCKLNSGDIVDF 105
Cdd:pfam00498   1 VTIGRS-------PDCDIVLDDPSVSRRHAEIRYDGG-GRFYLEDLGSTNGTFVNGQRLGPE-----PVRLKDGDVIRL 66

forkhead associated (FHA) domain found in transcription factor 19 (TCF-19) and similar proteins; TCF-19, also called transcription factor SC1, was identified as a putative trans-activating factor with expression beginning at the late G1-S boundary in dividing cells. It also functions as a novel islet factor necessary for proliferation and survival in the INS-1 beta cell line. It plays an important role in susceptibility to both Type 1 Diabetes Mellitus (T1DM) and Type 2 Diabetes Mellitus (T2DM); it has been suggested that it may positively impact beta cell mass under conditions of beta cell stress and increased insulin demand. TCF-19 contains an N-terminal fork head association domain (FHA), a proline rich region, and a C-terminal plant homeodomain (PHD) finger. The FHA domain may serve as a nuclear signaling domain or as a phosphoprotein binding domain. The proline rich region is a common characteristic of trans-activating factors. The PHD finger may allow TCF-19 to interact with chromatin via methylated histone H3.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   5 ITLTPLNESFQTKKLVISPSTIYKIGRHTNKSTSPSPSNLFfnSKVLSRQHAELWLDKDTLS---VYIRDvKSSNGTFVN 81
Cdd:cd22685   8 IGLSASRSEPRDLYTFRPDLCEYRIGRNPEVCDVFLCSSQH--PNLISREHAEIHAERDGNGnwkVLIED-RSTNGTYVN 84

                        90       100
                ....*....|....*....|....*
gi 19112554  82 ETRLSPENkpsaPCKLNSGDIVDFG 106
Cdd:cd22685  85 DVRLQDGQ----RRELSDGDTITFG 105

forkhead associated (FHA) domain found in angiogenic factor with G patch and FHA domains 1 (AGGF1) and similar proteins; AGGF1, also called angiogenic factor VG5Q, or G patch domain-containing protein 7 (GPATC7), or vasculogenesis gene on 5q protein, is an angiogenic factor involved in vascular development, angiogenesis, specification of hemangioblasts, and differentiation of veins. It promotes angiogenesis and the proliferation of endothelial cells. It inhibits inflammatory effect and preserve vascular integrity in non-nervous system diseases. Mutated AGGF1 causes susceptibility to Klippel-Trenaunay syndrome, a vascular disorder. Increased AGGF1 expression is associated with tumor angiogenesis. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 19112554  52 SRQHAELWLDKDTLSVYIRDVKSSNGTFVNETRLSPENKPSAPCKLNSGDIVDFG 106
Cdd:cd22686  48 SKFHAEIYYDDDEQSYTIVDLGSQNGTYLNGVRISQPKEKSDPYPLTHGDELKIG 102

forkhead associated (FHA) domain found in kanadaptin and similar proteins; Kanadaptin, also called human lung cancer oncogene 3 protein (HLC-3), kidney anion exchanger adapter protein, or solute carrier family 4 anion exchanger member 1 adapter protein (SLC4A1AP), is a nuclear protein widely expressed in mammalian tissues. It was originally isolated as a kidney Cl-/HCO3- anion exchanger 1 (kAE1)-binding protein. It is a highly mobile nucleocytoplasmic shuttling and multilocalizing protein. Its role in mammalian cells remains unclear. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  51 LSRQHAEL----WLDKDTLSVYIRDVKSSNGTFVNETRLspenKPSAPCKLNSGDIVDFG 106
Cdd:cd22677  41 ISRYHAVLqyrgDADDHDGGFYLYDLGSTHGTFLNKQRI----PPKQYYRLRVGHVLKFG 96

forkhead associated (FHA) domain found in RING finger protein 8 (RNF8) and similar proteins; RNF8 is a telomere-associated E3 ubiquitin-protein ligase that plays an important role in DNA double-strand break (DSB) repair via histone ubiquitination. It is localized in the nucleus and interacts with class III E2s (UBE2E2, UbcH6, and UBE2E3), but not with other E2s (UbcH5, UbcH7, UbcH10, hCdc34, and hBendless). It recruits UBC13 for lysine 63-based self polyubiquitylation. Its deficiency causes neuronal pathology and cognitive decline, and its loss results in neuron degeneration. RNF8, together with RNF168, catalyzes a series of ubiquitylation events on substrates such as H2A and H2AX, with the H2AK13/15 ubiquitylation being particularly important for recruitment of repair factors p53-binding protein 1 (53BP1) or the RAP80-BRCA1 complex to sites of DSBs. Specially, RNF8 mediates the ubiquitination of gammaH2AX, and recruits 53BP1 and BRCA1 to DNA damage sites which promotes DNA damage response (DDR) and inhibits chromosomal instability. Moreover, RNF8 interacts with retinoid X receptor alpha (RXR alpha) and enhances its transcription-stimulating activity. It also regulates the rate of exit from mitosis and cytokinesis. RNF8 contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  29 IGRHTNKSTSPSPSNlffnSKVLSRQHAELWLDkDTLSVYIRDVKSSNGTFVNETRLspenKPSAPCKLNSGDIVDFGVD 108
Cdd:cd22663  25 VGRGLGVTYQLVSTC----PLMISRNHCVLKKN-DEGQWTIKDNKSLNGVWVNGERI----EPLKPYPLNEGDLIQLGVP 95

                ....*....
gi 19112554 109 IYNEDEIVH 117
Cdd:cd22663  96 PENKEPAEY 104

forkhead associated (FHA) domain found in Saccharomyces cerevisiae meiosis-specific serine/threonine-protein kinase MEK1 and similar proteins; MEK1 (EC 2.7.11.1), also known as MRE4, is a meiosis-specific protein kinase required for chromosome synapsis and meiotic recombination. The recruitment and activation of MEK1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the MEK1 FHA domain. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   4 VITLTPLNESFQTKKLVISPSTIYKIGRHTNkstspspSNLFFNSKVLSRQHAELW---LDKDTLS-VYIRDVkSSNGTF 79
Cdd:cd22670   1 VGKLEVSSPGSTDIVLPIYKNQVITIGRSPS-------CDIVINDPFVSRTHCRIYsvqFDESSAPlVYVEDL-SSNGTY 72

                        90       100       110
                ....*....|....*....|....*....|.
gi 19112554  80 VNeTRLSPENKPSApckLNSGDIVDFGVDIY 110
Cdd:cd22670  73 LN-GKLIGRNNTVL---LSDGDVIEIAHSAT 99

forkhead associated (FHA) domain found in Mycobacterium tuberculosis FHA domain-containing protein FhaA and similar proteins; FhaA regulates cell growth and peptidoglycan synthesis by binding to MviN. It may inhibit the late stages of peptidoglycan synthesis. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 19112554  50 VLSRQHAELWLDKDTlsVYIRDVKSSNGTFVNETRLSPenkpsaPCKLNSGDIVDFG 106
Cdd:cd22668  36 GVSRRHAEIRWDGQV--AHLTDLGSTNGTTVNNAPVTP------EWRLADGDVITLG 84

forkhead associated (FHA) domain found in Bdellovibrio bacteriovorus GGDEF domain protein DgcB and similar proteins; DgcB is a GGDEF enzyme that produces cyclic-di-GMP in response to an unknown stimulus. It appends the C-terminal GGDEF enzymatic domain with an N-terminal forkhead-associated (FHA) domain that acts as a consensus phosphopeptide sensor. The GGDEF and sensory FHA domains form an asymmetrical dimer.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  17 KKLVISPSTIYkIGRHTNkstspspSNLFFNSKVLSRQHAELWLDKDtlSVYIRDVKSSNGTFVNETRLSPEnkpsAPCK 96
Cdd:cd22682  13 KQFPITESTIV-IGRSVE-------SQVQIDDDSVSRYHAKLAVNPS--AVSIIDLGSTNGTIVNGKKIPKL----ASCD 78

                        90
                ....*....|....
gi 19112554  97 LNSGDIVDFGVDIY 110
Cdd:cd22682  79 LQNGDQIKIGNTIF 92

forkhead associated (FHA) domain found in forkhead-associated domain-containing protein 1 (FHAD1) and similar proteins; FHAD1, also called FHA domain-containing protein 1, is an uncharacterized FHA domain-containing protein. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 19112554  29 IGRHTnkstspspSNLFFNSKVLSRQHAELWLDKDTLSVYIRDVKSSNGTFVNETRLSpenkpSAPCKLNSGDIVDFG 106
Cdd:cd22700  20 IGREG--------CDLVLQSPGVEEQHAVIEYSEQENCFVLQDLNTAQGTYVNDCRIQ-----NAAVRLAPGDVLRFG 84

forkhead associated (FHA) domain found in Saccharomyces cerevisiae DNA damage response protein kinase DUN1 and similar proteins; DUN1 is a protein kinase that controls the DNA damage response in yeast. It phosphorylates SML1 on serine residues and cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress. It contains an FHA domain, which is a small phosphopeptide recognition module.

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 19112554  51 LSRQHAELWLDKDTLSVYirDVKSSNGTFVNETRLSPENKPsapckLNSGDIVDFG 106
Cdd:cd22683  40 ISRFHAELRLEQNGINVI--DNNSANGTFINGKRIKGKTYI-----LKNGDIIVFG 88

Predicted component of the type VI protein secretion system, contains a FHA domain [Signal transduction mechanisms, Intracellular trafficking, secretion, and vesicular transport];

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 19112554  47 NSKVLSRQHAELWLDKDTLsvYIRDVkSSNGTFVNE--TRLSPENkpsaPCKLNSGDIVDFG-----VDIYNEDE 114
Cdd:COG3456  43 PDRSVSRRHAEIRFRDGAF--CLTDL-STNGTFLNGsdHPLGPGR----PVRLRDGDRLRIGdyeirVEISGEDE 110

second forkhead associated (FHA) domain found in Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 and similar proteins; RAD53, also called CHEK2 homolog, or serine-protein kinase 1 (Spk1), is a nuclear protein kinase that phosphorylates proteins on serine, threonine, and tyrosine. It controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints and prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. It may be involved in the phosphorylation of RPH1. RAD53 contains two FHA domains. This model corresponds to the second one. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   6 TLTPLNESFQTkkLVISPSTIyKIGRHTNkstspspSNLFFNSKVLSRQHAELWLD---KDTLSVYIRDVkSSNGTFVNE 82
Cdd:cd22690   3 RLKSLNPSYPD--IELTQNTT-FIGRSKD-------CDEEITDPRISKHHCIITRKrsgKGLDDVYVTDT-STNGTFINN 71

                        90
                ....*....|....*....
gi 19112554  83 TRLSPENKpsapCKLNSGD 101
Cdd:cd22690  72 NRLGKGSQ----SLLQDGD 86

forkhead associated (FHA) domain found in mediator of DNA damage checkpoint protein 1 (MDC1) and similar proteins; MDC1, also called nuclear factor with BRCT domains 1 (NFBD1), is a nuclear chromatin-associated protein that is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S and G2/M phases of the cell cycle. It directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. MDC1 contains a forkhead-associated (FHA) domain and two BRCT domains, as well as an internal 41-amino acid repeat sequence. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 19112554  29 IGRHTNkstspspSNLFFNSKVLSRQHAELWLDKDTLSVYirDVKSSNGTFVNETRLSpenKPSAPCKLNSGDIVDFG 106
Cdd:cd22665  25 IGRDPS-------CSVVLPDKSVSKQHACIEVDGGTHLIE--DLGSTNGTRIGNKVRL---KPNVRYELIDGDLLLFG 90

forkhead associated (FHA) domain found in Arabidopsis thaliana Protein PARALLEL SPINDLE 1 (PS1) and similar proteins; PS1 is an FHA domain-containing protein required for normal spindle orientation at male meiosis II and normal formation of tetrad of microspores. It is not involved in female meiosis. Mutations in PS1 lead to the production of diploid pollen grains. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 19112554  29 IGRHTNkstspspSNLFFNSKVLSRQHAELWLDKDTLSVYIRDVKSSNGTFVNETRLspenKPSAPCKLNSGDIVDFG 106
Cdd:cd22691  33 VGRHPD-------CDIVLDHPSISRFHLEIRIIPSRRKITLTDLSSVHGTWVNGQRI----EPGVPVELEEGDTVRLG 99

forkhead associated (FHA) domain found in Smad nuclear-interacting protein 1 (SNIP1), FHA domain-containing protein DDL, and similar proteins; SNIP1 is an FHA domain-containing protein required for pre-mRNA splicing as a component of the spliceosome. It inhibits NF-kappaB signaling by competing for its binding to the C/H1 domain of CBP/p300 transcriptional co-activators. It is involved in microRNA (miRNA) biogenesis. SNIP1 is a regulator of the cell cycle and cyclin D1 expression and may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. This family also includes Arabidopsis thaliana FHA domain-containing protein DDL and similar proteins. DDL, also called protein DAWDLE, is involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. It may facilitate DCL1 to access or recognize primary miRNAs. DDL binds RNA non-specifically. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  19 LVISPSTIYKIGRHTNKSTSPS--PSNlffnskvlSRQHAEL----------WLDKDTLSVYIRDVKSSNGTFVNETRLs 86
Cdd:cd22676  15 IDIHRQSAYLIGRDRRVADIPLdhPSC--------SKQHAVIqfrevekrneGDVIENIRPYIIDLGSTNGTFLNGEKI- 85

                        90       100
                ....*....|....*....|
gi 19112554  87 penKPSAPCKLNSGDIVDFG 106
Cdd:cd22676  86 ---EPRRYYELREKDVLKFG 102

first forkhead associated (FHA) domain found in Mycobacterium tuberculosis ABC transporter ATP-binding/permease protein Rv1747 and similar proteins; Rv1747 is a putative ATP-binding cassette (ABC) transporter involved in the translocation of an unknown substrate across the membrane. It is required for normal virulent infection by M. tuberculosis. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). Recruitment and phosphorylation of Rv1747 depend on the interaction between its two non-redundant FHA domains and the autophosphorylated form of serine/threonine protein kinase PknF. This model corresponds to the first FHA domain. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554  10 LNESFQTKKLVISPSTIYKIGRhtnkstspSPSN-LFFNSKVLSRQHAELWLDKDTLsvYIRDVKSSNGTFVNETRLSpe 88
Cdd:cd22694   1 LTIRIPGGELRFDPGSSVRIGR--------DPDAdVRLDDPRVSRRHALLEFDGDGW--VYTDLGSRNGTYLNGRRVQ-- 68

                        90
                ....*....|....*...
gi 19112554  89 nkpsaPCKLNSGDIVDFG 106
Cdd:cd22694  69 -----QVKLSDGTRVRLG 81

forkhead associated (FHA) domain found in aprataxin, bifunctional polynucleotide phosphatase/kinase (PNKP), aprataxin and PNK-like factor (APLF), and similar proteins; The family includes aprataxin, PNKP, and APLF. Aprataxin (EC 3.6.1.71/EC 3.6.1.72), also called forkhead-associated domain histidine triad-like protein (FHA-HIT), is a DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair, and base excision repair. It catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. It can also hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, it catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA but has higher specific activity with 5'-linked adenosine (AppDNA). PNKP (EC 3.1.3.32/EC 2.7.1.78), also called DNA 5'-kinase/3'-phosphatase, or polynucleotide kinase-3'-phosphatase, plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNKP ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone. APLF, also called apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, or XRCC1-interacting protein 1 (XIP1), is a novel apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease with conserved zinc-finger-like motifs involved in single-strand and double-strand DNA break repair. It is recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. It can introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, APLF promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Members of this family contain an FHA domain at their N-terminus. The FHA domain is a small phosphopeptide recognition module.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112554   6 TLTPLNESfQTKKLVISPSTIYKIGRhtnkstspspSNLFF-NSKVLSRQHAELWLDKDTLSVYIRdVKSSNGTFVNETR 84
Cdd:cd22671   1 FLRPVDGG-GGPPIELKEGGPTVLGR----------GPLLGiRDKRVSRKQAEITVDDDTGSVTVT-QLGTNPSFVNRAD 68

                        90       100
                ....*....|....*....|....*..
gi 19112554  85 LSPEN-KPSAPCKLNSGDIVDFGVDIY 110
Cdd:cd22671  69 GEGKVlKKGESVELKDGDVISLLPGKY 95