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Conserved domains on  [gi|224593260|ref|NP_689826|]
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postacrosomal sheath WW domain-binding protein [Homo sapiens]

Protein Classification

GRAM domain-containing protein; WBP2 family GRAM domain-containing protein( domain architecture ID 12987386)

GRAM domain-containing protein is a membrane-associated protein; similar to Macaca fascicularis postacrosomal sheath WW domain-binding protein that may play a role in meiotic resumption and pronuclear formation, mediated by a WW domain-signaling pathway during fertilization; WBP2 family GRAM domain-containing protein similar to Homo sapiens WW domain-binding protein 2 (WBP-2) that acts as a transcriptional coactivator of estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PH-GRAM_WBP2 cd13214
WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase ...
 39-134 3.35e-37

WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; WBP2 plays a number of roles including: acting as a tyrosine kinase substrate, activation of estrogen receptor alpha (ERalpha)/progesterone receptor (PR) transcription, and playing a role in breast cancer. WBP2 contain a N-terminal PH-GRAM domain and a C-terminal WWbp domain. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold. The WWbp domain is characterized by several short PY and PT-like motifs of the PPPPY form and binds to WW domains. WW domains contain two highly conserved tryptophans that are spaced 20-23 residues apart. They bind proline-rich peptide motifs [AP]-P-P-[AP]-Y, and/or phosphoserine- phosphothreonine-containing motifs.


:

Pssm-ID: 275401  Cd Length: 103  Bit Score: 128.06  E-value: 3.35e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260  39 GSNVFSGRKTGTLFLTSYRVIFITSCSiSDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFR 118
Cdd:cd13214    9 APPFFKGSKKGTIYLTNQRLIFVSSKP-TDNFQSFSMPLLNIRDVKFEQPVFGANYLKGKVKPVPGGGWPGEAEFKLTFK 87

                         90
                 ....*....|....*.
gi 224593260 119 NGDAIEFAQLMVKAAS 134
Cdd:cd13214   88 DGGAIEFGQAFLRLAE 103
PTZ00436 super family cl33183
60S ribosomal protein L19-like protein; Provisional
 200-305 9.94e-10

60S ribosomal protein L19-like protein; Provisional


The actual alignment was detected with superfamily member PTZ00436:

Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 58.81  E-value: 9.94e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 200 PVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAG 279
Cdd:PTZ00436 243 PAKAAAAPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAAAPAKAAAAPAKAAAPPAK 322

                         90       100
                 ....*....|....*....|....*.
gi 224593260 280 SGARPqeSTAAQAPENEASLPSASSS 305
Cdd:PTZ00436 323 AAAPP--AKAATPPAKAAAPPAKAAA 346
 
Name Accession Description Interval E-value
PH-GRAM_WBP2 cd13214
WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase ...
 39-134 3.35e-37

WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; WBP2 plays a number of roles including: acting as a tyrosine kinase substrate, activation of estrogen receptor alpha (ERalpha)/progesterone receptor (PR) transcription, and playing a role in breast cancer. WBP2 contain a N-terminal PH-GRAM domain and a C-terminal WWbp domain. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold. The WWbp domain is characterized by several short PY and PT-like motifs of the PPPPY form and binds to WW domains. WW domains contain two highly conserved tryptophans that are spaced 20-23 residues apart. They bind proline-rich peptide motifs [AP]-P-P-[AP]-Y, and/or phosphoserine- phosphothreonine-containing motifs.


Pssm-ID: 275401  Cd Length: 103  Bit Score: 128.06  E-value: 3.35e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260  39 GSNVFSGRKTGTLFLTSYRVIFITSCSiSDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFR 118
Cdd:cd13214    9 APPFFKGSKKGTIYLTNQRLIFVSSKP-TDNFQSFSMPLLNIRDVKFEQPVFGANYLKGKVKPVPGGGWPGEAEFKLTFK 87

                         90
                 ....*....|....*.
gi 224593260 119 NGDAIEFAQLMVKAAS 134
Cdd:cd13214   88 DGGAIEFGQAFLRLAE 103
PTZ00436 PTZ00436
60S ribosomal protein L19-like protein; Provisional
 200-305 9.94e-10

60S ribosomal protein L19-like protein; Provisional


Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 58.81  E-value: 9.94e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 200 PVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAG 279
Cdd:PTZ00436 243 PAKAAAAPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAAAPAKAAAAPAKAAAPPAK 322

                         90       100
                 ....*....|....*....|....*.
gi 224593260 280 SGARPqeSTAAQAPENEASLPSASSS 305
Cdd:PTZ00436 323 AAAPP--AKAATPPAKAAAPPAKAAA 346
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 45-135 7.24e-09

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 52.75  E-value: 7.24e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260   45 GRKTGTLFLTSYRVIFiTScSISDPMLSFMMPFDLMTNltVEQPVFAANFIKGTIQAAPYGGWEgqATFKLVFRNGDAIE 124
Cdd:pfam02893  28 GPVQGRLYLTNYRLCF-RS-LPKGWSTKVVIPLVDIEE--IEKLKGGANLFPNGIQVETGSNDK--FSFAGFVTRDEAIE 101

                          90
                  ....*....|.
gi 224593260  125 FAQLMVKAASA 135
Cdd:pfam02893 102 FILALLKNAHP 112
 
Name Accession Description Interval E-value
PH-GRAM_WBP2 cd13214
WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase ...
 39-134 3.35e-37

WW binding protein 2 (WB2) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; WBP2 plays a number of roles including: acting as a tyrosine kinase substrate, activation of estrogen receptor alpha (ERalpha)/progesterone receptor (PR) transcription, and playing a role in breast cancer. WBP2 contain a N-terminal PH-GRAM domain and a C-terminal WWbp domain. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold. The WWbp domain is characterized by several short PY and PT-like motifs of the PPPPY form and binds to WW domains. WW domains contain two highly conserved tryptophans that are spaced 20-23 residues apart. They bind proline-rich peptide motifs [AP]-P-P-[AP]-Y, and/or phosphoserine- phosphothreonine-containing motifs.


Pssm-ID: 275401  Cd Length: 103  Bit Score: 128.06  E-value: 3.35e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260  39 GSNVFSGRKTGTLFLTSYRVIFITSCSiSDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFR 118
Cdd:cd13214    9 APPFFKGSKKGTIYLTNQRLIFVSSKP-TDNFQSFSMPLLNIRDVKFEQPVFGANYLKGKVKPVPGGGWPGEAEFKLTFK 87

                         90
                 ....*....|....*.
gi 224593260 119 NGDAIEFAQLMVKAAS 134
Cdd:cd13214   88 DGGAIEFGQAFLRLAE 103
PTZ00436 PTZ00436
60S ribosomal protein L19-like protein; Provisional
 200-305 9.94e-10

60S ribosomal protein L19-like protein; Provisional


Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 58.81  E-value: 9.94e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 200 PVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAG 279
Cdd:PTZ00436 243 PAKAAAAPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAAAPAKAAAAPAKAAAPPAK 322

                         90       100
                 ....*....|....*....|....*.
gi 224593260 280 SGARPqeSTAAQAPENEASLPSASSS 305
Cdd:PTZ00436 323 AAAPP--AKAATPPAKAAAPPAKAAA 346
PTZ00436 PTZ00436
60S ribosomal protein L19-like protein; Provisional
 190-300 5.89e-09

60S ribosomal protein L19-like protein; Provisional


Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 56.50  E-value: 5.89e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 190 GAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGP 269
Cdd:PTZ00436 240 AAAPAKAAAAPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAAAPAKAAAAPAKAAAP 319

                         90       100       110
                 ....*....|....*....|....*....|.
gi 224593260 270 PAGYRASPAGSGARPqeSTAAQAPENEASLP 300
Cdd:PTZ00436 320 PAKAAAPPAKAATPP--AKAAAPPAKAAAAP 348
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 45-135 7.24e-09

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 52.75  E-value: 7.24e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260   45 GRKTGTLFLTSYRVIFiTScSISDPMLSFMMPFDLMTNltVEQPVFAANFIKGTIQAAPYGGWEgqATFKLVFRNGDAIE 124
Cdd:pfam02893  28 GPVQGRLYLTNYRLCF-RS-LPKGWSTKVVIPLVDIEE--IEKLKGGANLFPNGIQVETGSNDK--FSFAGFVTRDEAIE 101

                          90
                  ....*....|.
gi 224593260  125 FAQLMVKAASA 135
Cdd:pfam02893 102 FILALLKNAHP 112
PTZ00436 PTZ00436
60S ribosomal protein L19-like protein; Provisional
 189-305 1.57e-08

60S ribosomal protein L19-like protein; Provisional


Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 55.34  E-value: 1.57e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 189 YGAPPAGYGAqPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEG 268
Cdd:PTZ00436 226 AAAPAKAAAP-PAKAAAAPAKAAAAPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAA 304

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 224593260 269 PPAGYRASPAGSGARPqeSTAAQAPENEASLPSASSS 305
Cdd:PTZ00436 305 APAKAAAAPAKAAAPP--AKAAAPPAKAATPPAKAAA 339
PTZ00436 PTZ00436
60S ribosomal protein L19-like protein; Provisional
 182-282 6.92e-06

60S ribosomal protein L19-like protein; Provisional


Pssm-ID: 185616 [Multi-domain]  Cd Length: 357  Bit Score: 47.25  E-value: 6.92e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 182 YGAPPPGYGAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGA 261
Cdd:PTZ00436 253 AAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAPPAKAAAAPAKAAAAPAKAAAAPAKAAAPPAKAAAPPAKAAT 332

                         90       100
                 ....*....|....*....|.
gi 224593260 262 PPAGNEGPPAGYRASPAGSGA 282
Cdd:PTZ00436 333 PPAKAAAPPAKAAAAPVGKKA 353
PH-GRAM cd10570
Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins ...
 45-90 2.00e-03

Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; Myotubularin-related proteins are a subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3-phosphorylated inositol lipids. Mutations in this family cause the human neuromuscular disorders myotubular myopathy and type 4B Charcot-Marie-Tooth syndrome. 6 of the 13 MTMRs (MTMRs 5, 9-13) contain naturally occurring substitutions of residues required for catalysis by PTP family enzymes. Although these proteins are predicted to be enzymatically inactive, they are thought to function as antagonists of endogenous phosphatase activity or interaction modules. Most MTMRs contain a N-terminal PH-GRAM domain, a Rac-induced recruitment domain (RID) domain, a PTP domain (which may be active or inactive), a SET-interaction domain, and a C-terminal coiled-coil region. In addition some members contain DENN domain N-terminal to the PH-GRAM domain and FYVE, PDZ, and PH domains C-terminal to the coiled-coil region. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275393  Cd Length: 94  Bit Score: 36.98  E-value: 2.00e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 224593260  45 GRKTGTLFLTSYRVIFITscSISDPMLSFMMPFDLMTNLTVEQPVF 90
Cdd:cd10570   17 LPLEGTLYLSTYRLIFSS--KADGDETKLVIPLVDITDVEKIAGAS 60
PRK07764 PRK07764
DNA polymerase III subunits gamma and tau; Validated
 199-306 2.25e-03

DNA polymerase III subunits gamma and tau; Validated


Pssm-ID: 236090 [Multi-domain]  Cd Length: 824  Bit Score: 39.58  E-value: 2.25e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 224593260 199 QPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLG-YGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASP 277
Cdd:PRK07764 620 APAAPAAPAPAGAAAAPAEASAAPAPGVAAPEHHPKHVAVPDASDGgDGWPAKAGGAAPAAPPPAPAPAAPAAPAGAAPA 699

                         90       100
                 ....*....|....*....|....*....
gi 224593260 278 AGSGARPQESTAAQAPENEASLPSASSSQ 306
Cdd:PRK07764 700 QPAPAPAATPPAGQADDPAAQPPQAAQGA 728
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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