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Conserved domains on  [gi|27436891|ref|NP_758436|]
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GDP-fucose protein O-fucosyltransferase 1 isoform 2 precursor [Homo sapiens]

Protein Classification

O-fucosyltransferase family protein( domain architecture ID 94843)

O-fucosyltransferase family protein may be involved in glycan metabolism by O-fucosylation of protein substrates

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
O-FucT_like super family cl16914
GDP-fucose protein O-fucosyltransferase and related proteins; O-fucosyltransferase-like ...
 30-181 1.65e-92

GDP-fucose protein O-fucosyltransferase and related proteins; O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes.


The actual alignment was detected with superfamily member cd11302:

Pssm-ID: 450121  Cd Length: 347  Bit Score: 273.72  E-value: 1.65e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27436891  30 DPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPftNLHVSYQKYFKLEPLQAYHRVISLEDFM 109
Cdd:cd11302   1 DPNGYILYCPCMGRFGNQADHFLGSLAFAKALNRTLVLPPWIEYRHGPPP--SVQIPFDDYFKVEPLQEYHRVITMEDFM 78

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 27436891 110 EKLAPTHWPPEKRVAYCFEVAAqrSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELF---TGISFSASYREQWSQR 181
Cdd:cd11302  79 EELAPTIWPPGKRKGYCYSPRA--SPDSKDCPMKEGNPFGPFWDHFGVDFDGSELYgplSYDTFYPDVREAWNER 151
 
Name Accession Description Interval E-value
O-FucT-1 cd11302
GDP-fucose protein O-fucosyltransferase 1; The protein O-fucosyltransferase 1 (Ofut1 or ...
 30-181 1.65e-92

GDP-fucose protein O-fucosyltransferase 1; The protein O-fucosyltransferase 1 (Ofut1 or O-FucT-1) adds O-fucose to EGF (epidermal growth factor-like) repeats. The O-fucsosylation of the Notch receptor signaling protein is dependent on this enzyme, which requires GDP-fucose as a substrate. O-fucose residues added to the target of O-FucT-1 may be further elongated by other glycosyltransferases. On top of O-fucosylation, O-FucT-1 may have other functions such as the regulation of the Notch receptor exit from the ER. Six highly conserved cysteines are present in O-FucT-1, which is a soluble ER protein, as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The membrane-bound pre-protein is released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. O-FucT-1 is similar to family 1 glycosyltransferases (GT1).


Pssm-ID: 211388  Cd Length: 347  Bit Score: 273.72  E-value: 1.65e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27436891  30 DPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPftNLHVSYQKYFKLEPLQAYHRVISLEDFM 109
Cdd:cd11302   1 DPNGYILYCPCMGRFGNQADHFLGSLAFAKALNRTLVLPPWIEYRHGPPP--SVQIPFDDYFKVEPLQEYHRVITMEDFM 78

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 27436891 110 EKLAPTHWPPEKRVAYCFEVAAqrSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELF---TGISFSASYREQWSQR 181
Cdd:cd11302  79 EELAPTIWPPGKRKGYCYSPRA--SPDSKDCPMKEGNPFGPFWDHFGVDFDGSELYgplSYDTFYPDVREAWNER 151
O-FucT pfam10250
GDP-fucose protein O-fucosyltransferase; This is a family of conserved proteins representing ...
 34-161 3.65e-27

GDP-fucose protein O-fucosyltransferase; This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteriztic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese.


Pssm-ID: 463023 [Multi-domain]  Cd Length: 247  Bit Score: 103.15  E-value: 3.65e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27436891    34 YLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNlHVSYQKYFKLeplqayhrvisledFMEKLa 113
Cdd:pfam10250   1 YLLYCPCNGGFNQQRDHICDAVAFARLLNATLVLPPWDQLYHWRDPSTD-QIPFSDIFDE--------------FIESL- 64

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 27436891   114 pthwppekrvaycfevaaqrspdkktCPMKEGNpFGPFWDQFH-VSFNK 161
Cdd:pfam10250  65 --------------------------CRSKQGN-FGPFWVNFHaLRFSP 86
 
Name Accession Description Interval E-value
O-FucT-1 cd11302
GDP-fucose protein O-fucosyltransferase 1; The protein O-fucosyltransferase 1 (Ofut1 or ...
 30-181 1.65e-92

GDP-fucose protein O-fucosyltransferase 1; The protein O-fucosyltransferase 1 (Ofut1 or O-FucT-1) adds O-fucose to EGF (epidermal growth factor-like) repeats. The O-fucsosylation of the Notch receptor signaling protein is dependent on this enzyme, which requires GDP-fucose as a substrate. O-fucose residues added to the target of O-FucT-1 may be further elongated by other glycosyltransferases. On top of O-fucosylation, O-FucT-1 may have other functions such as the regulation of the Notch receptor exit from the ER. Six highly conserved cysteines are present in O-FucT-1, which is a soluble ER protein, as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteristic of several glycosyltransferase families. The membrane-bound pre-protein is released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese. O-FucT-1 is similar to family 1 glycosyltransferases (GT1).


Pssm-ID: 211388  Cd Length: 347  Bit Score: 273.72  E-value: 1.65e-92
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27436891  30 DPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPftNLHVSYQKYFKLEPLQAYHRVISLEDFM 109
Cdd:cd11302   1 DPNGYILYCPCMGRFGNQADHFLGSLAFAKALNRTLVLPPWIEYRHGPPP--SVQIPFDDYFKVEPLQEYHRVITMEDFM 78

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 27436891 110 EKLAPTHWPPEKRVAYCFEVAAqrSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELF---TGISFSASYREQWSQR 181
Cdd:cd11302  79 EELAPTIWPPGKRKGYCYSPRA--SPDSKDCPMKEGNPFGPFWDHFGVDFDGSELYgplSYDTFYPDVREAWNER 151
O-FucT pfam10250
GDP-fucose protein O-fucosyltransferase; This is a family of conserved proteins representing ...
 34-161 3.65e-27

GDP-fucose protein O-fucosyltransferase; This is a family of conserved proteins representing the enzyme responsible for adding O-fucose to EGF (epidermal growth factor-like) repeats. Six highly conserved cysteines are present in O-FucT-1 as well as a DXD-like motif (ERD), conserved in mammals, Drosophila, and C. elegans. Both features are characteriztic of several glycosyltransferase families. The enzyme is a membrane-bound protein released by proteolysis and, as for most glycosyltransferases, is strongly activated by manganese.


Pssm-ID: 463023 [Multi-domain]  Cd Length: 247  Bit Score: 103.15  E-value: 3.65e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 27436891    34 YLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNlHVSYQKYFKLeplqayhrvisledFMEKLa 113
Cdd:pfam10250   1 YLLYCPCNGGFNQQRDHICDAVAFARLLNATLVLPPWDQLYHWRDPSTD-QIPFSDIFDE--------------FIESL- 64

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 27436891   114 pthwppekrvaycfevaaqrspdkktCPMKEGNpFGPFWDQFH-VSFNK 161
Cdd:pfam10250  65 --------------------------CRSKQGN-FGPFWVNFHaLRFSP 86
O-FucT_like cd11296
GDP-fucose protein O-fucosyltransferase and related proteins; O-fucosyltransferase-like ...
 34-69 4.00e-07

GDP-fucose protein O-fucosyltransferase and related proteins; O-fucosyltransferase-like proteins are GDP-fucose dependent enzymes with similarities to the family 1 glycosyltransferases (GT1). They are soluble ER proteins that may be proteolytically cleaved from a membrane-associated preprotein, and are involved in the O-fucosylation of protein substrates, the core fucosylation of growth factor receptors, and other processes.


Pssm-ID: 211383  Cd Length: 206  Bit Score: 48.18  E-value: 4.00e-07
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 27436891  34 YLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPP 69
Cdd:cd11296   1 YLLPIPDGGGFNNQRNEFLNALLLAILLGRTLVLPL 36
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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