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Conserved domains on  [gi|489328149|ref|WP_003235436|]
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MULTISPECIES: ribosome hibernation promotion factor [Bacillus]

Protein Classification

HPF/RaiA family ribosome-associated protein( domain architecture ID 10794669)

HPF/RaiA family ribosome-associated protein such as Mycobacterium tuberculosis ribosome hibernation promotion factor (HPF), which is required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
yfiA TIGR00741
ribosomal subunit interface protein; This model includes a small protein encoded by one of two ...
 1-98 2.29e-39

ribosomal subunit interface protein; This model includes a small protein encoded by one of two genes, both downstream of the gene rpoN for sigma 54, whose deletion leads to increased expression from sigma 54-dependent promoters. It also includes the N-terminal half of a light-repressed protein LtrA of Synechococcus PCC 7002 and the N-terminal region (after removal of the transit peptide) of a larger plastid-specific ribosomal protein of spinach. The member of this family from E. coli is now recognized as a protein at the interace between ribosomal large and small subunits, with about 1/3 as many copies per cell as the number of ribosomes. [Protein synthesis, Translation factors]


:

Pssm-ID: 129824 [Multi-domain]  Cd Length: 95  Bit Score: 129.76  E-value: 2.29e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149    1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNLKFYNdkeSKVEVTIPMTDLALRSEVHNEDMYNAIDLA 80
Cdd:TIGR00741   1 MNINITGNNVEITEALREYVEEKLARLERYFTRIINVDVVLSVERNR---FKVEATLYVNGGVIRASAESEDMYAAIDLA 77

                          90
                  ....*....|....*...
gi 489328149   81 TNKLERQIRKHKTKVNRK 98
Cdd:TIGR00741  78 IDKLERQLRKLKEKRKEH 95
Ribosom_S30AE_C pfam16321
Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the ...
 133-184 6.13e-29

Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the C-terminus of proteins containing pfam02482.


:

Pssm-ID: 435275  Cd Length: 54  Bit Score: 101.71  E-value: 6.13e-29
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 489328149  133 IVRQKRFNLKPMDSEEAILQMNMLGHNFFVFTNAETNLTNVVYRRNDGKYGL 184
Cdd:pfam16321   3 IVRTKRFPLKPMSVEEAVLQMELLGHDFFVFRNAETGEVNVVYRRKDGNYGL 54
 
Name Accession Description Interval E-value
yfiA TIGR00741
ribosomal subunit interface protein; This model includes a small protein encoded by one of two ...
 1-98 2.29e-39

ribosomal subunit interface protein; This model includes a small protein encoded by one of two genes, both downstream of the gene rpoN for sigma 54, whose deletion leads to increased expression from sigma 54-dependent promoters. It also includes the N-terminal half of a light-repressed protein LtrA of Synechococcus PCC 7002 and the N-terminal region (after removal of the transit peptide) of a larger plastid-specific ribosomal protein of spinach. The member of this family from E. coli is now recognized as a protein at the interace between ribosomal large and small subunits, with about 1/3 as many copies per cell as the number of ribosomes. [Protein synthesis, Translation factors]


Pssm-ID: 129824 [Multi-domain]  Cd Length: 95  Bit Score: 129.76  E-value: 2.29e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149    1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNLKFYNdkeSKVEVTIPMTDLALRSEVHNEDMYNAIDLA 80
Cdd:TIGR00741   1 MNINITGNNVEITEALREYVEEKLARLERYFTRIINVDVVLSVERNR---FKVEATLYVNGGVIRASAESEDMYAAIDLA 77

                          90
                  ....*....|....*...
gi 489328149   81 TNKLERQIRKHKTKVNRK 98
Cdd:TIGR00741  78 IDKLERQLRKLKEKRKEH 95
RaiA COG1544
Ribosome-associated translation inhibitor RaiA [Translation, ribosomal structure and ...
 2-104 1.53e-36

Ribosome-associated translation inhibitor RaiA [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441153  Cd Length: 101  Bit Score: 122.58  E-value: 1.53e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   2 NYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNvnLKFYNDKESKVEVTIPMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:COG1544    1 KINITGRNVEVTDALREYVEEKLEKLEKYFDRIISADVT--LSVEKNRRFKVEITLHVPGPVLRAEAEAEDMYAAIDLAV 78

                         90       100
                 ....*....|....*....|...
gi 489328149  82 NKLERQIRKHKTKVNRKFREQGS 104
Cdd:COG1544   79 DKLERQLRKYKEKLKDHRRRRAP 101
RaiA cd00552
RaiA ("ribosome-associated inhibitor A", also known as Protein Y (PY), YfiA, and SpotY, is a ...
 2-94 4.69e-36

RaiA ("ribosome-associated inhibitor A", also known as Protein Y (PY), YfiA, and SpotY, is a stress-response protein that binds the ribosomal subunit interface and arrests translation by interfering with aminoacyl-tRNA binding to the ribosomal A site. RaiA is also thought to counteract miscoding at the A site thus reducing translation errors. The RaiA fold structurally resembles the double-stranded RNA-binding domain (dsRBD).


Pssm-ID: 238308  Cd Length: 93  Bit Score: 121.08  E-value: 4.69e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   2 NYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNLKFYNDKESKVEVTIPMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:cd00552    1 NINITGRNIEVTDALREYVEEKLEKLEKYFDRIISVDVVLSVEKNHGKRFKVEITIHLPGGVLRAEASAEDLYAAIDLAV 80

                         90
                 ....*....|...
gi 489328149  82 NKLERQIRKHKTK 94
Cdd:cd00552   81 DKLERQLRKYKEK 93
Ribosom_S30AE_C pfam16321
Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the ...
 133-184 6.13e-29

Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the C-terminus of proteins containing pfam02482.


Pssm-ID: 435275  Cd Length: 54  Bit Score: 101.71  E-value: 6.13e-29
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 489328149  133 IVRQKRFNLKPMDSEEAILQMNMLGHNFFVFTNAETNLTNVVYRRNDGKYGL 184
Cdd:pfam16321   3 IVRTKRFPLKPMSVEEAVLQMELLGHDFFVFRNAETGEVNVVYRRKDGNYGL 54
Ribosomal_S30AE pfam02482
Sigma 54 modulation protein / S30EA ribosomal protein; This Pfam family contains the sigma-54 ...
 3-95 2.31e-28

Sigma 54 modulation protein / S30EA ribosomal protein; This Pfam family contains the sigma-54 modulation protein family and the S30AE family of ribosomal proteins which includes the light- repressed protein (lrtA).


Pssm-ID: 460569  Cd Length: 87  Bit Score: 101.43  E-value: 2.31e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149    3 YNIRGENIEVTPALKDHVEKKIGKLERYFdhsvnADVNVNLKfyNDKESKVEVTI-PMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:pfam02482   1 INITGRNIEVTDALREYVEKKLEKLEKYF-----ISADVTLS--VEKRHKVEVTIrLVPGVVLRAEETAEDLYAAIDLAV 73

                          90
                  ....*....|....
gi 489328149   82 NKLERQIRKHKTKV 95
Cdd:pfam02482  74 DKLERQLRKYKEKL 87
PRK10470 PRK10470
ribosome hibernation promoting factor;
1-97 5.22e-14

ribosome hibernation promoting factor;


Pssm-ID: 182484  Cd Length: 95  Bit Score: 64.54  E-value: 5.22e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHsVNaDVNVNLKFynDKESKV-EVTIPMTDLALRSEVHNEDMYNAIDL 79
Cdd:PRK10470   1 MQLNITGHNVEITEALREFVTAKFAKLEQYFDR-IN-QVYVVLKV--EKVTHIsDATLHVNGGEIHASAEGQDMYAAIDG 76

                         90
                 ....*....|....*...
gi 489328149  80 ATNKLERQIRKHKTKVNR 97
Cdd:PRK10470  77 LIDKLARQLTKHKDKLKQ 94
 
Name Accession Description Interval E-value
yfiA TIGR00741
ribosomal subunit interface protein; This model includes a small protein encoded by one of two ...
 1-98 2.29e-39

ribosomal subunit interface protein; This model includes a small protein encoded by one of two genes, both downstream of the gene rpoN for sigma 54, whose deletion leads to increased expression from sigma 54-dependent promoters. It also includes the N-terminal half of a light-repressed protein LtrA of Synechococcus PCC 7002 and the N-terminal region (after removal of the transit peptide) of a larger plastid-specific ribosomal protein of spinach. The member of this family from E. coli is now recognized as a protein at the interace between ribosomal large and small subunits, with about 1/3 as many copies per cell as the number of ribosomes. [Protein synthesis, Translation factors]


Pssm-ID: 129824 [Multi-domain]  Cd Length: 95  Bit Score: 129.76  E-value: 2.29e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149    1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNLKFYNdkeSKVEVTIPMTDLALRSEVHNEDMYNAIDLA 80
Cdd:TIGR00741   1 MNINITGNNVEITEALREYVEEKLARLERYFTRIINVDVVLSVERNR---FKVEATLYVNGGVIRASAESEDMYAAIDLA 77

                          90
                  ....*....|....*...
gi 489328149   81 TNKLERQIRKHKTKVNRK 98
Cdd:TIGR00741  78 IDKLERQLRKLKEKRKEH 95
RaiA COG1544
Ribosome-associated translation inhibitor RaiA [Translation, ribosomal structure and ...
 2-104 1.53e-36

Ribosome-associated translation inhibitor RaiA [Translation, ribosomal structure and biogenesis];


Pssm-ID: 441153  Cd Length: 101  Bit Score: 122.58  E-value: 1.53e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   2 NYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNvnLKFYNDKESKVEVTIPMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:COG1544    1 KINITGRNVEVTDALREYVEEKLEKLEKYFDRIISADVT--LSVEKNRRFKVEITLHVPGPVLRAEAEAEDMYAAIDLAV 78

                         90       100
                 ....*....|....*....|...
gi 489328149  82 NKLERQIRKHKTKVNRKFREQGS 104
Cdd:COG1544   79 DKLERQLRKYKEKLKDHRRRRAP 101
RaiA cd00552
RaiA ("ribosome-associated inhibitor A", also known as Protein Y (PY), YfiA, and SpotY, is a ...
 2-94 4.69e-36

RaiA ("ribosome-associated inhibitor A", also known as Protein Y (PY), YfiA, and SpotY, is a stress-response protein that binds the ribosomal subunit interface and arrests translation by interfering with aminoacyl-tRNA binding to the ribosomal A site. RaiA is also thought to counteract miscoding at the A site thus reducing translation errors. The RaiA fold structurally resembles the double-stranded RNA-binding domain (dsRBD).


Pssm-ID: 238308  Cd Length: 93  Bit Score: 121.08  E-value: 4.69e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   2 NYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNLKFYNDKESKVEVTIPMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:cd00552    1 NINITGRNIEVTDALREYVEEKLEKLEKYFDRIISVDVVLSVEKNHGKRFKVEITIHLPGGVLRAEASAEDLYAAIDLAV 80

                         90
                 ....*....|...
gi 489328149  82 NKLERQIRKHKTK 94
Cdd:cd00552   81 DKLERQLRKYKEK 93
Ribosom_S30AE_C pfam16321
Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the ...
 133-184 6.13e-29

Sigma 54 modulation/S30EA ribosomal protein C terminus; This domain often occurs at the C-terminus of proteins containing pfam02482.


Pssm-ID: 435275  Cd Length: 54  Bit Score: 101.71  E-value: 6.13e-29
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 489328149  133 IVRQKRFNLKPMDSEEAILQMNMLGHNFFVFTNAETNLTNVVYRRNDGKYGL 184
Cdd:pfam16321   3 IVRTKRFPLKPMSVEEAVLQMELLGHDFFVFRNAETGEVNVVYRRKDGNYGL 54
Ribosomal_S30AE pfam02482
Sigma 54 modulation protein / S30EA ribosomal protein; This Pfam family contains the sigma-54 ...
 3-95 2.31e-28

Sigma 54 modulation protein / S30EA ribosomal protein; This Pfam family contains the sigma-54 modulation protein family and the S30AE family of ribosomal proteins which includes the light- repressed protein (lrtA).


Pssm-ID: 460569  Cd Length: 87  Bit Score: 101.43  E-value: 2.31e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149    3 YNIRGENIEVTPALKDHVEKKIGKLERYFdhsvnADVNVNLKfyNDKESKVEVTI-PMTDLALRSEVHNEDMYNAIDLAT 81
Cdd:pfam02482   1 INITGRNIEVTDALREYVEKKLEKLEKYF-----ISADVTLS--VEKRHKVEVTIrLVPGVVLRAEETAEDLYAAIDLAV 73

                          90
                  ....*....|....
gi 489328149   82 NKLERQIRKHKTKV 95
Cdd:pfam02482  74 DKLERQLRKYKEKL 87
PRK10470 PRK10470
ribosome hibernation promoting factor;
1-97 5.22e-14

ribosome hibernation promoting factor;


Pssm-ID: 182484  Cd Length: 95  Bit Score: 64.54  E-value: 5.22e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHsVNaDVNVNLKFynDKESKV-EVTIPMTDLALRSEVHNEDMYNAIDL 79
Cdd:PRK10470   1 MQLNITGHNVEITEALREFVTAKFAKLEQYFDR-IN-QVYVVLKV--EKVTHIsDATLHVNGGEIHASAEGQDMYAAIDG 76

                         90
                 ....*....|....*...
gi 489328149  80 ATNKLERQIRKHKTKVNR 97
Cdd:PRK10470  77 LIDKLARQLTKHKDKLKQ 94
PRK10324 PRK10324
ribosome-associated translation inhibitor RaiA;
1-97 1.86e-11

ribosome-associated translation inhibitor RaiA;


Pssm-ID: 182378  Cd Length: 113  Bit Score: 58.57  E-value: 1.86e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 489328149   1 MNYNIRGENIEVTPALKDHVEKKIGKLERYFDHSVNADVNVNlkfyndKESK---VEVTIPMTDLALRSEVHNEDMYNAI 77
Cdd:PRK10324   1 MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILS------KEPQgfvADATINTPNGVLVASGKHEDMYTAI 74

                         90       100
                 ....*....|....*....|..
gi 489328149  78 DLATNKLERQIRK--HKTKVNR 97
Cdd:PRK10324  75 NELINKLERQLNKlqHKGEARR 96
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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