Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   614 VFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEF 693
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   694 ISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGD-IPEDLVPLVKNQVFEFLI----------RL 762
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSDLEEDDEENANKVFPYLSYILTGRQYPTGEgLPEDEASKAKSEIYSFLFsgtsiewppgSG 160

                          170       180       190
                   ....*....|....*....|....*....|....
gi 530374200   763 HSAEASPEEEIYPYIRTLLHFDTREFLNVLALTF 796
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLNEAF 194

Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   614 VFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEF 693
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   694 ISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGD-IPEDLVPLVKNQVFEFLI----------RL 762
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSDLEEDDEENANKVFPYLSYILTGRQYPTGEgLPEDEASKAKSEIYSFLFsgtsiewppgSG 160

                          170       180       190
                   ....*....|....*....|....*....|....
gi 530374200   763 HSAEASPEEEIYPYIRTLLHFDTREFLNVLALTF 796
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLNEAF 194

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200  197 GQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITdAHPpgTAILHIKFTDDPTLAICNDSGGSVfeltfkRV 276
Cdd:cd00200   175 GHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLR-GHE--NGVNSVAFSPDGYLLASGSEDGTI------RV 245

                          90       100
                  ....*....|....*....|..
gi 530374200  277 MGVRTCESRCLFSGSKGEVCCI 298
Cdd:cd00200   246 WDLRTGECVQTLSGHTNSVTSL 267

Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   614 VFLECLEPYILSDKLVGITPQVMKDLIVHFQDKKLMENVEALIVHMDITSLDIQQVVLMCWENRLYDAMIYVYNRGMNEF 693
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200   694 ISPMEKLFRVIAPPLNAGKTLTDEQVVMGNKLLVYISCCLAGRAYPLGD-IPEDLVPLVKNQVFEFLI----------RL 762
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSDLEEDDEENANKVFPYLSYILTGRQYPTGEgLPEDEASKAKSEIYSFLFsgtsiewppgSG 160

                          170       180       190
                   ....*....|....*....|....*....|....
gi 530374200   763 HSAEASPEEEIYPYIRTLLHFDTREFLNVLALTF 796
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLNEAF 194

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 530374200  197 GQYGAISALSINNDCSRLLCGFAKGQITMWDLASGKLLRSITdAHPpgTAILHIKFTDDPTLAICNDSGGSVfeltfkRV 276
Cdd:cd00200   175 GHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLR-GHE--NGVNSVAFSPDGYLLASGSEDGTI------RV 245

                          90       100
                  ....*....|....*....|..
gi 530374200  277 MGVRTCESRCLFSGSKGEVCCI 298
Cdd:cd00200   246 WDLRTGECVQTLSGHTNSVTSL 267