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Conserved domains on  [gi|564329358|ref|XP_006229614|]
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isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial isoform X1 [Rattus norvegicus]

Protein Classification

NAD-dependent isocitrate dehydrogenase( domain architecture ID 10015520)

NAD-dependent isocitrate dehydrogenase converts isocitrate to 2-oxoglutarate in an NAD-dependent manner

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 48-379 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


:

Pssm-ID: 272942  Cd Length: 333  Bit Score: 586.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 365
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 564329358  366 GTTSQAIQDIIRHI 379
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 48-379 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 586.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 365
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 564329358  366 GTTSQAIQDIIRHI 379
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 34-379 5.31e-131

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 379.98  E-value: 5.31e-131
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  34 APRRSISSPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKG 112
Cdd:PLN00123  14 AQRRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKG 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 113 NIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAK 192
Cdd:PLN00123  92 GLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFC 169

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:PLN00123 170 SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLY 249

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 273 GNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVL 349
Cdd:PLN00123 250 GNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVK 329

                        330       340       350
                 ....*....|....*....|....*....|
gi 564329358 350 ASMDNENMHTPDIGGQGTTSQAIQDIIRHI 379
Cdd:PLN00123 330 RVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 50-379 9.35e-118

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 345.84  E-value: 9.35e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  50 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 117
Cdd:COG0473    1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 118 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 185
Cdd:COG0473   77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 186 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:COG0473  155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 340
Cdd:COG0473  234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 564329358 341 ATSIRKAVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 379
Cdd:COG0473  309 ADAIEAAVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
52-375 4.18e-89

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 272.63  E-value: 4.18e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   52 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 123
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  124 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 192
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  273 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 350
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319

                         330       340
                  ....*....|....*....|....*...
gi 564329358  351 SMDNENMhTPDIGGQGT---TSQAIQDI 375
Cdd:pfam00180 320 VLESGIR-TGDLAGSATyvsTSEFGEAV 346
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 48-379 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 586.66  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   48 GGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHK 125
Cdd:TIGR00175   1 GGKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  126 SRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  286 GPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 365
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 564329358  366 GTTSQAIQDIIRHI 379
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 34-379 5.31e-131

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 379.98  E-value: 5.31e-131
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  34 APRRSISSPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKG 112
Cdd:PLN00123  14 AQRRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKG 91

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 113 NIETNHNLPPShkSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAK 192
Cdd:PLN00123  92 GLATPVGGGVS--SLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFC 169

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:PLN00123 170 SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLY 249

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 273 GNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVL 349
Cdd:PLN00123 250 GNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVK 329

                        330       340       350
                 ....*....|....*....|....*....|
gi 564329358 350 ASMDNENMHTPDIGGQGTTSQAIQDIIRHI 379
Cdd:PLN00123 330 RVIAEGKYRTKDLGGSSTTQEVVDAVIANL 359
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 52-379 1.85e-124

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 363.82  E-value: 1.85e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  52 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSR 127
Cdd:PLN00118  43 TATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSL 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 128 NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQES 207
Cdd:PLN00118 121 NLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTH 200

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 208 GRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGP 287
Cdd:PLN00118 201 GRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGL 280

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 288 GLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGT 367
Cdd:PLN00118 281 GLTPSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLKLNEQAEQIHNAILNTIAEGKYRTADLGGSST 360

                        330
                 ....*....|..
gi 564329358 368 TSQAIQDIIRHI 379
Cdd:PLN00118 361 TTDFTKAICDHL 372
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 50-379 9.35e-118

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 345.84  E-value: 9.35e-118
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  50 RHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETN 117
Cdd:COG0473    1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGP 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 118 HnlPPSHKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESL 185
Cdd:COG0473   77 K--WDDGVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDT 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 186 KIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:COG0473  155 RVYTRKGIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDV 233

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSY 340
Cdd:COG0473  234 IVTENLFGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEA 308

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 564329358 341 ATSIRKAVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 379
Cdd:COG0473  309 ADAIEAAVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 52-379 3.32e-108

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 320.90  E-value: 3.32e-108
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  52 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSR 127
Cdd:PRK08997   4 TITVIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSI 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 128 NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQ 205
Cdd:PRK08997  82 NVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELAR 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 206 ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 285
Cdd:PRK08997 162 KEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVG 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 286 GPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQ 365
Cdd:PRK08997 242 GLGMAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDLGGT 320

                        330
                 ....*....|....
gi 564329358 366 GTTSQAIQDIIRHI 379
Cdd:PRK08997 321 HGTTDFTQAVIDRL 334
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
53-370 3.13e-100

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 305.66  E-value: 3.13e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SH 124
Cdd:PRK09222   7 ITVAYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGY 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 125 KSRNNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:PRK09222  82 KSLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 204 AQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 283
Cdd:PRK09222 162 ARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEI 241

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 284 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIG 363
Cdd:PRK09222 242 SGSVGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLE-DGIHTADIY 319


                 ....*..
gi 564329358 364 GQGTTSQ 370
Cdd:PRK09222 320 NEGVSKK 326
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
52-375 4.18e-89

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 272.63  E-value: 4.18e-89
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   52 TVTMIPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPS 123
Cdd:pfam00180   1 KIAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  124 HKSRNNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAK 192
Cdd:pfam00180  81 VRPENGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDE 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  193 SLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:pfam00180 161 IERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLF 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  273 GNIVNNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLA 350
Cdd:pfam00180 241 GDILSDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLK 319

                         330       340
                  ....*....|....*....|....*...
gi 564329358  351 SMDNENMhTPDIGGQGT---TSQAIQDI 375
Cdd:pfam00180 320 VLESGIR-TGDLAGSATyvsTSEFGEAV 346
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 51-379 1.86e-81

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 252.36  E-value: 1.86e-81
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  51 HTVTMIPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppS 123
Cdd:PRK14025   2 HKICVIEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------T 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 124 HKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:PRK14025  73 AADVIVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEM 152

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 204 AQ----ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNV 279
Cdd:PRK14025 153 AKrrkkMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDG 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 280 CAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHT 359
Cdd:PRK14025 233 AAGLVGGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLA-LGLTT 310

                        330       340
                 ....*....|....*....|
gi 564329358 360 PDIGGQGTTSQAIQDIIRHI 379
Cdd:PRK14025 311 PDLGGNLSTMEMAEEVAKRV 330
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 53-376 9.50e-79

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 245.44  E-value: 9.50e-79
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSH 124
Cdd:TIGR02088   1 VAVIPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGY 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  125 KSRNNILRTSLDLYANVIHCKSLPGVVTRH-KDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKL 203
Cdd:TIGR02088  75 KSVIVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  204 AQESGRKkVTAVHKANIMKLGDGLFLQCCREVAARYpQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGL 283
Cdd:TIGR02088 154 AKERNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASAL 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  284 VGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPDIG 363
Cdd:TIGR02088 232 AGSLGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDLG 309

                         330
                  ....*....|...
gi 564329358  364 GQGTTSQAIQDII 376
Cdd:TIGR02088 310 GTAKTKEVGDEIA 322
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 53-370 1.30e-77

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 247.37  E-value: 1.30e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   53 VTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSR 127
Cdd:TIGR02924   3 ITVAYGDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  128 NNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 206
Cdd:TIGR02924  81 NVTLRKTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARK 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  207 SGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 286
Cdd:TIGR02924 161 HNRKKVTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGS 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  287 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGGQG 366
Cdd:TIGR02924 241 VGLAGSANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLE-DGVHTADIYNEK 318


                  ....
gi 564329358  367 TTSQ 370
Cdd:TIGR02924 319 TSKQ 322
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 51-380 4.63e-54

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 182.61  E-value: 4.63e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  51 HTVTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NL 120
Cdd:PRK00772   3 YKIAVLPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNL 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 121 PPSHKSRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhKDIDILIVRENTEGEY-------SSLEHESVAgv 181
Cdd:PRK00772  83 PPDVRPERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA-- 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 182 VESLkIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQ 261
Cdd:PRK00772 158 FDTM-VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPK 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 262 QFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH-LK 336
Cdd:PRK00772 235 QFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRYsLG 310

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*..
gi 564329358 337 LHSYATSIRKAVLASMDnENMHTPDI---GGQGTTSQAIQDIIRHIR 380
Cdd:PRK00772 311 LEEAADAIEAAVEKVLA-QGYRTADIaegGGKVSTSEMGDAILAALA 356
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 55-363 5.39e-47

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 163.56  E-value: 5.39e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  55 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 116
Cdd:PRK03437   9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 117 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 185
Cdd:PRK03437  88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 186 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDV 265
Cdd:PRK03437 156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 266 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 342
Cdd:PRK03437 235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAA 313

                        330       340
                 ....*....|....*....|....
gi 564329358 343 SIRKAV---LASMDNENMHTPDIG 363
Cdd:PRK03437 314 RIEAAVeadLAERGKMGRSTAEVG 337
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 52-380 5.89e-47

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 163.74  E-value: 5.89e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  52 TVTMIPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHN 119
Cdd:PRK08194   5 KIAVIPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 120 LPPSHKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHKDIDILIVRENTEGEYSSL-------EHEsvagVVESLK 186
Cdd:PRK08194  81 LVPDHISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNA 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 187 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccREVAARYPQITFDSMIVDNTTMQLVSRPQQ 262
Cdd:PRK08194 157 VFTRKGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEE 231

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 263 FDVMVMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKlHS 339
Cdd:PRK08194 232 FDVIVASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHFG-EE 309

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|.
gi 564329358 340 YATSIRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIR 380
Cdd:PRK08194 310 ELGSHLLDVIEDVTEDGIKTPDIGGRATTDEVTDEIISRLK 350
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 53-379 1.32e-45

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 159.87  E-value: 1.32e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358   53 VTMIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPP 122
Cdd:TIGR00169   2 IAVLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  123 SHKSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------KDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAK 192
Cdd:TIGR00169  82 DQRPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPE 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  193 SLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLY 272
Cdd:TIGR00169 162 IERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLF 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  273 GNIVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLAS 351
Cdd:TIGR00169 240 GDILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEAAVKK 318

                         330       340
                  ....*....|....*....|....*...
gi 564329358  352 MDNENMHTPDIGGQGTTSQAIQDIIRHI 379
Cdd:TIGR00169 319 VLAEGYRTPDLGSSATTAVGTAEMGEEL 346
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
56-362 1.67e-42

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 153.45  E-value: 1.67e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  56 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 124
Cdd:PRK06451  29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 125 KSRNNILRTSLDLYANVIHCKSLPGVVTRHKD---IDILIVRENTEGEYSSLEH----------------------ESVA 179
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 180 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR--------------------- 238
Cdd:PRK06451 187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVALKefrdyvvteeevtknyngvpp 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 239 YPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 318
Cdd:PRK06451 265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 564329358 319 ANPTATLLASCMMLDHLKLHSYATSIRKAVLASMdNENMHTPDI 362
Cdd:PRK06451 344 ANPTGIIKGGELMLRFMGWDKAADLIDKAIMESI-KQKKVTQDL 386
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 56-380 1.66e-41

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 150.65  E-value: 1.66e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  56 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 122
Cdd:COG0538   24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 123 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 185
Cdd:COG0538   99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 186 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR-YP-------------- 240
Cdd:COG0538  179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEEeFGdkfitegpwekykg 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 241 -----QITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 310
Cdd:COG0538  259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 564329358 311 ksIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPD----IGGQG--TTSQAIQDIIRHIR 380
Cdd:COG0538  336 --YAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDlarlMEGATelSTSEFGDAIIENLD 408
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 102-348 5.55e-31

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 122.09  E-value: 5.55e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 102 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEH 175
Cdd:PRK07006  84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 176 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 230
Cdd:PRK07006 159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 231 CCREVAARY--------------------PQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 290
Cdd:PRK07006 237 WGYQLAEEEfgdelidggpwdkiknpetgKEIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 564329358 291 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 348
Cdd:PRK07006 317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSM 373
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 37-366 4.50e-26

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 108.24  E-value: 4.50e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358  37 RSISSPPSAKyggRHTVTMIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRV 108
Cdd:PLN02329  36 RCAAASPGKK---RYNIALLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDA 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 109 ALKGNI------ETNHNLPPSHKSRNniLRTSLDLYANVIHCKSLPGVV---TRHKDI----DILIVRENTEGEYSSLE- 174
Cdd:PLN02329 113 ILLGAIggykwdKNEKHLRPEMALFY--LRRDLKVFANLRPATVLPQLVdasTLKKEVaegvDMMIVRELTGGIYFGEPr 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 175 ----HESVAGVVESLKIITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFDSMIVD 250
Cdd:PLN02329 191 gitiNENGEEVGVSTEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVD 268

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 251 NTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCM 330
Cdd:PLN02329 269 NAAMQLIRDPKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAM 348

                        330       340       350
                 ....*....|....*....|....*....|....*..
gi 564329358 331 MLDH-LKLHSYATSIRKAVLASMdNENMHTPDIGGQG 366
Cdd:PLN02329 349 LLKYgLGEEKAAKRIEDAVVDAL-NKGFRTGDIYSPG 384
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
100-354 8.53e-22

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 96.71  E-value: 8.53e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 100 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSSL 173
Cdd:PRK07362  91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 174 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 223
Cdd:PRK07362 166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 224 GDGLFLQCCREVAA--------------------RYPQIT-----------FDSM------------------------- 247
Cdd:PRK07362 246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISiednarmiepgYDSLtpekkaaicaevkevldsiwsshgn 325

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329358 248 ------------IVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 315
Cdd:PRK07362 326 gkwkekvlvddrIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 564329358 316 KNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDN 354
Cdd:PRK07362 405 LDRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIAN 443
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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