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Conserved domains on  [gi|564329362|ref|XP_006229616|]
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isocitrate dehydrogenase [NAD] subunit gamma 1, mitochondrial isoform X2 [Rattus norvegicus]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
1-325 0e+00

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 567.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHKSRNNILR 78
Cdd:TIGR00175   8 LIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHRSLNVALR 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   79 TSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKV 158
Cdd:TIGR00175  87 KELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARKNGRKKV 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  159 TAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAG 238
Cdd:TIGR00175 167 TAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVGGPGLVPG 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  239 ANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAI 318
Cdd:TIGR00175 247 ANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGTATTSDFT 326


                  ....*..
gi 564329362  319 QDIIRHI 325
Cdd:TIGR00175 327 EAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 1-325 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 567.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHKSRNNILR 78
Cdd:TIGR00175   8 LIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHRSLNVALR 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   79 TSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKV 158
Cdd:TIGR00175  87 KELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARKNGRKKV 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  159 TAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAG 238
Cdd:TIGR00175 167 TAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVGGPGLVPG 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  239 ANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAI 318
Cdd:TIGR00175 247 ANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGTATTSDFT 326


                  ....*..
gi 564329362  319 QDIIRHI 325
Cdd:TIGR00175 327 EAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 1-325 7.99e-127

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 367.27  E-value: 7.99e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLPPShkSRNNILRT 79
Cdd:PLN00123  35 LIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGGVS--SLNVQLRK 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  80 SLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVT 159
Cdd:PLN00123 111 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVT 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 160 AVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGA 239
Cdd:PLN00123 191 AVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGG 270

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 240 NYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00123 271 NVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQE 350


                 ....*....
gi 564329362 317 AIQDIIRHI 325
Cdd:PLN00123 351 VVDAVIANL 359
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 2-325 1.29e-116

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 340.84  E-value: 1.29e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETNHnlPPS 69
Cdd:COG0473    7 LPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGPK--WDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  70 HKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESLKIITKA 137
Cdd:COG0473   81 GVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 138 KSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNL 217
Cdd:COG0473  161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIVTENL 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 218 YGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSYATSIRK 292
Cdd:COG0473  240 FGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEAADAIEA 314

                        330       340       350
                 ....*....|....*....|....*....|...
gi 564329362 293 AVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:COG0473  315 AVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
2-321 1.77e-88

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 269.16  E-value: 1.77e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    2 IPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPSHKSR 73
Cdd:pfam00180   5 LPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAGVRPE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   74 NNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAKSLRI 142
Cdd:pfam00180  85 NGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEIERI 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  143 AEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV 222
Cdd:pfam00180 165 ARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFGDIL 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  223 NNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLASMDN 300
Cdd:pfam00180 245 SDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKVLES 323

                         330       340
                  ....*....|....*....|....
gi 564329362  301 ENMhTPDIGGQGT---TSQAIQDI 321
Cdd:pfam00180 324 GIR-TGDLAGSATyvsTSEFGEAV 346
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 1-325 0e+00

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 567.40  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNAD--EEDIRNAIMAIRRNRVALKGNIETNHNLPpSHKSRNNILR 78
Cdd:TIGR00175   8 LIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDgkTEIPDEAVESIKRNKVALKGPLETPIGKG-GHRSLNVALR 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   79 TSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKV 158
Cdd:TIGR00175  87 KELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYARKNGRKKV 166

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  159 TAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAG 238
Cdd:TIGR00175 167 TAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVGGPGLVPG 246

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  239 ANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQAI 318
Cdd:TIGR00175 247 ANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGTATTSDFT 326


                  ....*..
gi 564329362  319 QDIIRHI 325
Cdd:TIGR00175 327 EAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 1-325 7.99e-127

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 367.27  E-value: 7.99e-127
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSnaDEEDIRNAIM-AIRRNRVALKGNIETNHNLPPShkSRNNILRT 79
Cdd:PLN00123  35 LIPGDGIGPLVTGAVEQVMEAMHAPVYFERYEVHG--DMKKVPEEVLeSIRRNKVCLKGGLATPVGGGVS--SLNVQLRK 110

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  80 SLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVT 159
Cdd:PLN00123 111 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVT 190

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 160 AVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGA 239
Cdd:PLN00123 191 AVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGG 270

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 240 NYGHVYAVFETATR--NTGK-SIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00123 271 NVGADHAVFEQGASagNVGNeKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGSSTTQE 350


                 ....*....
gi 564329362 317 AIQDIIRHI 325
Cdd:PLN00123 351 VVDAVIANL 359
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 1-325 5.49e-124

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 360.35  E-value: 5.49e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNI 76
Cdd:PLN00118  46 LFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTTVDPRTgsflTWESLESVRRNKVGLKGPMATP--IGKGHRSLNLT 123

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  77 LRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRK 156
Cdd:PLN00118 124 LRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRK 203

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 157 KVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 236
Cdd:PLN00118 204 RVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLT 283

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 237 AGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSQ 316
Cdd:PLN00118 284 PSCNIGENGLALAEAVHGSAPDIAGKNLANPTALLLSAVMMLRHLKLNEQAEQIHNAILNTIAEGKYRTADLGGSSTTTD 363


                 ....*....
gi 564329362 317 AIQDIIRHI 325
Cdd:PLN00118 364 FTKAICDHL 372
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 2-325 1.29e-116

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 340.84  E-value: 1.29e-116
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA--------DEEDIRnaimAIRRNRVALKGNIETNHnlPPS 69
Cdd:COG0473    7 LPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEADIGGAAydktgtplPDETLE----ALRKADAILLGAVGGPK--WDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  70 HKSRNNI---LRTSLDLYANVIHCKSLPGVVTRHK-----DIDILIVRENTEGEYSSLEHESVAG----VVESLKIITKA 137
Cdd:COG0473   81 GVRPESGllaLRKELDLYANLRPAKLYPGLPSPLKpeiveGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRVYTRK 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 138 KSLRIAEYAFKLAQESgRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNL 217
Cdd:COG0473  161 GIERIARYAFELARKR-RKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIVTENL 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 218 YGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntgksIANKNIANPTATLLASCMMLDHLKLHSYATSIRK 292
Cdd:COG0473  240 FGDILSDLAAGLTGSLGLAPSANIGdEGKALFEpvhgSAPD-----IAGKGIANPIATILSAAMMLRHLGEEEAADAIEA 314

                        330       340       350
                 ....*....|....*....|....*....|...
gi 564329362 293 AVLASMDnENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:COG0473  315 AVEKVLA-EGVRTPDLGGKAGTSEMGDAIIAAL 346
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 1-325 3.13e-106

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 313.96  E-value: 3.13e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDI----RNAIMAIRRNRVALKGNIETNhnLPPSHKSRNNI 76
Cdd:PRK08997   7 VIPGDGIGPSIIDATLKILDKLGCDFEYEFADAGLTALEKHGellpQRTLDLIEKNKIALKGPLTTP--VGEGFTSINVT 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  77 LRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHE-SVAG-VVESLKIITKAKSLRIAEYAFKLAQESG 154
Cdd:PRK08997  85 LRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYELARKEG 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 155 RKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPG 234
Cdd:PRK08997 165 RKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAGLVGGLG 244

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 235 LVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTT 314
Cdd:PRK08997 245 MAPGANIGRDAAIFE-AVHGSAPDIAGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRDLGGTHGT 323

                        330
                 ....*....|.
gi 564329362 315 SQAIQDIIRHI 325
Cdd:PRK08997 324 TDFTQAVIDRL 334
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
2-316 3.87e-99

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 300.65  E-value: 3.87e-99
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETnhnlpP---SHKSR 73
Cdd:PRK09222  10 AYGDGIGPEIMEAVLKILEAAGAPLEIETIEIGEKVYKKGWTSGISpsaweSIRRTKVLLKAPITT-----PqggGYKSL 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  74 NNILRTSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE 152
Cdd:PRK09222  85 NVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPGSEKIIRYAFEYARA 164

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 153 SGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 232
Cdd:PRK09222 165 NGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLYGDILSDIAAEISGS 244

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 233 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGGQG 312
Cdd:PRK09222 245 VGLAGSANIGEEYAMFE-AVHGSAPDIAGKNIANPSGLLNAAVMMLVHIGQFDIAELIENAWLKTLE-DGIHTADIYNEG 322


                 ....
gi 564329362 313 TTSQ 316
Cdd:PRK09222 323 VSKK 326
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
2-321 1.77e-88

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 269.16  E-value: 1.77e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    2 IPGDGIGPELMLHVKSVFRHACVPV----DFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNIETNHNLPPSHKSR 73
Cdd:pfam00180   5 LPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAiDETGEplpDETLEACKKADAVLLGAVGGPKWDPAGVRPE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   74 NNI--LRTSLDLYANVIHCKSLPGVVTRHK------DIDILIVRENTEGEYSSLEHE---SVAGVVESLKIITKAKSLRI 142
Cdd:pfam00180  85 NGLlaLRKELGLFANLRPAKVFPPLGDASPlkneveGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEIERI 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  143 AEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV 222
Cdd:pfam00180 165 ARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFGDIL 244

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  223 NNVCAGLVGGPGLVAGANYG-HVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKAVLASMDN 300
Cdd:pfam00180 245 SDEASMLTGSLGLLPSASLGaNGFGIFE-PVHGSAPDIAGKGIANPIATILSAAMMLRYsLGLEDAADKIEAAVLKVLES 323

                         330       340
                  ....*....|....*....|....
gi 564329362  301 ENMhTPDIGGQGT---TSQAIQDI 321
Cdd:pfam00180 324 GIR-TGDLAGSATyvsTSEFGEAV 346
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 2-325 1.53e-80

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 248.12  E-value: 1.53e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRhaCVPVDFEEVHVSSnADEEDIR-------NAIMAIRRNRVALKGNIETnhnlppSHKSRN 74
Cdd:PRK14025   7 IEGDGIGKEVVPAALHVLE--ATGLPFEFVYAEA-GDEVFEKtgkalpeETIEAAKEADAVLFGAAGE------TAADVI 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  75 NILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQ--- 151
Cdd:PRK14025  78 VKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEMAKrrk 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 152 -ESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLV 230
Cdd:PRK14025 158 kMGKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDGAAGLV 237

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 231 GGPGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGG 310
Cdd:PRK14025 238 GGLGLAPSANIGDKYGLFE-PVHGSAPDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLA-LGLTTPDLGG 315

                        330
                 ....*....|....*
gi 564329362 311 QGTTSQAIQDIIRHI 325
Cdd:PRK14025 316 NLSTMEMAEEVAKRV 330
LEU3_arch TIGR02088
isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal ...
 2-322 2.63e-78

isopropylmalate/isohomocitrate dehydrogenases; This model represents a group of archaeal decarboxylating dehydrogenases which include the leucine biosynthesis enzyme 3-isopropylmalate dehydrogenase (LeuB, LEU3) and the methanogenic cofactor CoB biosynthesis enzyme isohomocitrate dehydrogenase (AksF). Both of these have been characterized in Methanococcus janaschii. Non-methanogenic archaea have only one hit to this model and presumably this is LeuB, although phylogenetic trees cannot establish which gene is which in the methanogens. The AksF gene is capable of acting on isohomocitrate, iso(homo)2-citrate and iso(homo)3-citrate in the successive elongation cycles of coenzyme B (7-mercaptoheptanoyl-threonine phosphate). This family is closely related to both the LeuB genes found in TIGR00169 and the mitochondrial eukaryotic isocitrate dehydratases found in TIGR00175. All of these are included within the broader subfamily model, pfam00180.


Pssm-ID: 273962  Cd Length: 322  Bit Score: 242.36  E-value: 2.63e-78
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    2 IPGDGIGPELMLHVKSVFRHACVPVDFEEVH--------VSSNADEEDIRnaimAIRRNRVALKGNIETNhnLPPSHKSR 73
Cdd:TIGR02088   4 IPGDGIGPEVIEAAIRILNKLGLEIEFIEFEagdealkkYGSALPEDTLE----EIRKADAILFGAVTTP--ANPGYKSV 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   74 NNILRTSLDLYANVIHCKSLPGVVTRH-KDIDILIVRENTEGEYSSLEhESVAGVVESLKIITKAKSLRIAEYAFKLAQE 152
Cdd:TIGR02088  78 IVTLRKELDLYANVRPAKSLPGIPDLYpNGKDIVIVRENTEGLYAGFE-FGFSDRAIAIRVITREGSERIARFAFNLAKE 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  153 SGRKkVTAVHKANIMKLGDGLFLQCCREVAARYpQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGG 232
Cdd:TIGR02088 157 RNRK-VTCVHKANVLKGTDGLFREVCREIAKRY-GVEYRDMYVDSAAMNLVKDPWRFDVIVTTNMFGDILSDLASALAGS 234

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  233 PGLVAGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPDIGGQG 312
Cdd:TIGR02088 235 LGLAPSANIGDRKALFE-PVHGSAPDIAGKGIANPTAAILSVAMMLDYLGELEKGKLVWEAVEYYIIEGKK-TPDLGGTA 312

                         330
                  ....*....|
gi 564329362  313 TTSQAIQDII 322
Cdd:TIGR02088 313 KTKEVGDEIA 322
ICDH_alpha TIGR02924
isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of ...
 4-316 1.61e-77

isocitrate dehydrogenase; This family of mainly alphaproteobacterial enzymes is a member of the isocitrate/isopropylmalate dehydrogenase superfamily described by pfam00180. Every member of the seed of this model appears to have a TCA cycle lacking only a determined isocitrate dehydrogenase. The precise identity of the cofactor (NADH -- 1.1.1.41 vs. NADPH -- 1.1.1.42) is unclear. [Energy metabolism, TCA cycle]


Pssm-ID: 274353 [Multi-domain]  Cd Length: 473  Bit Score: 245.06  E-value: 1.61e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    4 GDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIM-----AIRRNRVALKGNIETNHNlpPSHKSRNNILR 78
Cdd:TIGR02924   8 GDGIGPEIMEAVLLILKEAEAPIDIETIEIGEKVYKKGWPSGISpssweSIRRTKVLLKAPITTPQG--GGHKSLNVTLR 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   79 TSLDLYANVIHCKSL-PGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKK 157
Cdd:TIGR02924  86 KTLGLYANIRPCVSYhPFIETKSPNLNIVIVRENEEDLYTGIEYRQTPDTYECTKLITRSGSEKICRYAFEYARKHNRKK 165

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  158 VTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVA 237
Cdd:TIGR02924 166 VTCLTKDNIMKMTDGIFHKIFDKIAAEYPDIESEHYIVDIGMARLATNPENFDVIVTPNLYGDILSDVAAEISGSVGLAG 245

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 564329362  238 GANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDnENMHTPDIGGQGTTSQ 316
Cdd:TIGR02924 246 SANIGEEYAMFE-AVHGSAPDIAGQNIANPSGLLNAAIQMLVHIGQSDIAQLIYNAWLKTLE-DGVHTADIYNEKTSKQ 322
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 2-326 5.54e-54

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 180.68  E-value: 5.54e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNA-DE--EDIRNAIMAIRRNRVA-LKGNIETNH--NLPPSHK 71
Cdd:PRK00772   8 LPGDGIGPEVMaeaVKVlDAVAEKFGFDFEFEEALVGGAAiDAhgVPLPEETLEACRAADAvLLGAVGGPKwdNLPPDVR 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  72 SRNNIL--RTSLDLYAN---------VIHCKSL-PGVVtrhKDIDILIVRENTEGEY-------SSLEHESVAgvVESLk 132
Cdd:PRK00772  88 PERGLLalRKELGLFANlrpaklypgLADASPLkPEIV---AGLDILIVRELTGGIYfgeprgrEGLGGEERA--FDTM- 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 133 IITKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVM 212
Cdd:PRK00772 162 VYTREEIERIARVAFELARKR-RKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRNPKQFDVI 239

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 213 VMPNLYGNIVNNVCAGLVGGPGLVAGANYGHV-YAVFETAtrntGKS---IANKNIANPTATLLASCMMLDH-LKLHSYA 287
Cdd:PRK00772 240 VTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI----HGSapdIAGKGIANPIATILSAAMMLRYsLGLEEAA 315

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|..
gi 564329362 288 TSIRKAVLASMDnENMHTPDI---GGQGTTSQAIQDIIRHIR 326
Cdd:PRK00772 316 DAIEAAVEKVLA-QGYRTADIaegGGKVSTSEMGDAILAALA 356
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 1-309 1.32e-47

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 163.56  E-value: 1.32e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHACVP-VDFEEVH-------------VSSNADEEDIR--NAIM--AIRRNRVAlKGNIET 62
Cdd:PRK03437   9 VIPGDGIGPEVVAEALKVLDAVAAGgPGVETTEydlgarrylrtgeTLPDSVLAELRqhDAILlgAIGDPSVP-SGVLER 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  63 NHNLPpshksrnniLRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSS--------LEHEsVAGVVEsl 131
Cdd:PRK03437  88 GLLLK---------LRFALDHYVNLRPSKLYPGVTSPLAgpgDIDFVVVREGTEGPYTGnggalrvgTPHE-VATEVS-- 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 132 kIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDV 211
Cdd:PRK03437 156 -VNTAFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDV 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 212 MVMPNLYGNIVNNVCAGLVGGPGLVAGANY---GHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYAT 288
Cdd:PRK03437 235 IVTDNLFGDIITDLAAAVTGGIGLAASGNInptGTNPSMFE-PVHGSAPDIAGQGIADPTAAILSVALLLDHLGEEDAAA 313

                        330       340
                 ....*....|....*....|....
gi 564329362 289 SIRKAV---LASMDNENMHTPDIG 309
Cdd:PRK03437 314 RIEAAVeadLAERGKMGRSTAEVG 337
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 2-326 2.83e-47

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 162.97  E-value: 2.83e-47
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFR-----HACVPVDFEEVHVSSnadEEDIRNAIMAIRRNRVALKgNIET-------NHNLPPS 69
Cdd:PRK08194   9 IPGDGVGKEVVPAAVRVLKavaevHGGLKFEFTEFPWSC---EYYLEHGEMMPEDGLEQLK-QFDAiflgavgNPKLVPD 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  70 HKSRNNIL---RTSLDLYANVIHCKSLPGV---VTRHKDIDILIVRENTEGEYSSL-------EHEsvagVVESLKIITK 136
Cdd:PRK08194  85 HISLWGLLikiRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVggrihrgEDE----IAIQNAVFTR 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 137 AKSLRIAEYAFKLAQESgRKKVTAVHKAN----IMKLGDGLFlqccREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVM 212
Cdd:PRK08194 161 KGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEEFDVI 235

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 213 VMPNLYGNIVNNVCAGLVGGPGLVAGAN---YGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKlHSYATS 289
Cdd:PRK08194 236 VASNLFGDILTDIGAAIMGSIGIAPAANinvNGKYPSMFE-PVHGSAPDIAGKGIANPIGQIWTAKLMLDHFG-EEELGS 313

                        330       340       350
                 ....*....|....*....|....*....|....*..
gi 564329362 290 IRKAVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIR 326
Cdd:PRK08194 314 HLLDVIEDVTEDGIKTPDIGGRATTDEVTDEIISRLK 350
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 1-325 1.99e-45

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 157.95  E-value: 1.99e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362    1 MIPGDGIGPELM---LHV-KSVFRHACVPVDFEEVHVSSNADE---EDIRNAIMAIRRNRVA-LKGNIETNH--NLPPSH 70
Cdd:TIGR00169   4 VLPGDGIGPEVMaqaLKVlKAVAERFGLKFEFEEHLIGGAAIDatgQPLPEETLKGCKEADAvLLGAVGGPKwdNLPRDQ 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   71 KSRNNIL--RTSLDLYANVIHCKSLPGVVTRH-------KDIDILIVRENTEGEYSSLEHE-SVAGVVESLKIITKAKSL 140
Cdd:TIGR00169  84 RPEQGLLklRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYFGEPKGrEGEGEAWDTEVYTVPEIE 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  141 RIAEYAFKLAQESgRKKVTAVHKANIMKLGDgLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGN 220
Cdd:TIGR00169 164 RIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTSNLFGD 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  221 IVNNVCAGLVGGPGLVAGANYGH-VYAVFETAtRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMD 299
Cdd:TIGR00169 242 ILSDEASVITGSLGMLPSASLGSdGFGLFEPV-HGSAPDIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEAAVKKVL 320

                         330       340
                  ....*....|....*....|....*.
gi 564329362  300 NENMHTPDIGGQGTTSQAIQDIIRHI 325
Cdd:TIGR00169 321 AEGYRTPDLGSSATTAVGTAEMGEEL 346
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
2-308 8.86e-43

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 152.68  E-value: 8.86e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRHACV-------PVDFEEVHVSSNADEED----IRNAIMAIRRNRVALKGNIETNhnLPPSH 70
Cdd:PRK06451  29 VEGDGIGPEITHAAMKVINKAVEkaygsdrEIKWVEVLAGDKAEKLTgnrfPKESEELIEKYRVLLKGPLETP--IGKGW 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  71 KSRNNILRTSLDLYANVIHCKSLPGVVTRHKD---IDILIVRENTEGEYSSLEH----------------------ESVA 125
Cdd:PRK06451 107 KSINVAIRLMLDLYANIRPVKYIPGIESPLKNpekIDLIIFRENTDDLYRGIEYpydseeakkirdflrkelgvevEDDT 186

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 126 GVveSLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR--------------------- 184
Cdd:PRK06451 187 GI--GIKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAYEVALKefrdyvvteeevtknyngvpp 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 185 YPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIANKNI 264
Cdd:PRK06451 265 SGKVIINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE-AIHGTAPKYAGKNV 343

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....
gi 564329362 265 ANPTATLLASCMMLDHLKLHSYATSIRKAVLASMdNENMHTPDI 308
Cdd:PRK06451 344 ANPTGIIKGGELMLRFMGWDKAADLIDKAIMESI-KQKKVTQDL 386
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 2-326 7.58e-42

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 149.88  E-value: 7.58e-42
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   2 IPGDGIGPELMLHVKSVFRHA-------CVPVDFEEVHVS-SNADEEDIR---NAIMAIRRNRVALKGNIETnhnlpP-- 68
Cdd:COG0538   24 IEGDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGeKARDETGDWlpdETAEAIKEYGVGIKGPLTT-----Pvg 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  69 -SHKSRNNILRTSLDLYAN---VIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHES----VAGVVESL--------- 131
Cdd:COG0538   99 gGWRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvi 178

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 132 ----------KIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAAR-YP-------------- 186
Cdd:COG0538  179 rfpedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEEeFGdkfitegpwekykg 258

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 187 -----QITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYG-HVYAVFE----TATRntg 256
Cdd:COG0538  259 pkpagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGANIGdDGGAEFEathgTAPK--- 335

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 564329362 257 ksIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMhTPD----IGGQG--TTSQAIQDIIRHIR 326
Cdd:COG0538  336 --YAGKDSTNPGSLILSGTMMLRHRGWLEAADLIEKAVEKTIESGKV-TYDlarlMEGATelSTSEFGDAIIENLD 408
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 48-294 1.88e-31

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 122.09  E-value: 1.88e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  48 AIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYA---NVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEH 121
Cdd:PRK07006  84 LIREYRVAIKGPLTT-----PvggGIRSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEW 158

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 122 EsvAGVVESLKII-------------------------TKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQ 176
Cdd:PRK07006 159 K--AGSAEAKKVIkflqeemgvkkirfpetsgigikpvSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKD 236

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 177 CCREVAARY--------------------PQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLV 236
Cdd:PRK07006 237 WGYQLAEEEfgdelidggpwdkiknpetgKEIIVKDSIADAFLQQILLRPAEYDVIATMNLNGDYISDALAAQVGGIGIA 316

                        250       260       270       280       290
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 564329362 237 AGANYGHVYAVFEtATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAV 294
Cdd:PRK07006 317 PGANINDGHAIFE-ATHGTAPKYAGLDKVNPGSVILSAEMMLRHMGWTEAADLIIKSM 373
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 1-312 1.14e-24

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 103.23  E-value: 1.14e-24
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362   1 MIPGDGIGPELMLHVKSVFRHAC----VPVDFEEVHVSSNA-DEEDI---RNAIMAIRRNRVALKGNI------ETNHNL 66
Cdd:PLN02329  51 LLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVplpEETFTAAKQSDAILLGAIggykwdKNEKHL 130

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  67 PPSHKSRNniLRTSLDLYANVIHCKSLPGVV---TRHKDI----DILIVRENTEGEYSSLE-----HESVAGVVESLKII 134
Cdd:PLN02329 131 RPEMALFY--LRRDLKVFANLRPATVLPQLVdasTLKKEVaegvDMMIVRELTGGIYFGEPrgitiNENGEEVGVSTEIY 208

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 135 TKAKSLRIAEYAFKLAQESgRKKVTAVHKANIMKlGDGLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVM 214
Cdd:PLN02329 209 AAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRDPKQFDTIVT 286

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 215 PNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDH-LKLHSYATSIRKA 293
Cdd:PLN02329 287 NNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEEKAAKRIEDA 366

                        330
                 ....*....|....*....
gi 564329362 294 VLASMdNENMHTPDIGGQG 312
Cdd:PLN02329 367 VVDAL-NKGFRTGDIYSPG 384
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
46-300 3.14e-22

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 96.71  E-value: 3.14e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362  46 IMAIRRNRVALKGNIETnhnlpP---SHKSRNNILRTSLDLYANVIHCKSLPGVVTRHK---DIDILIVRENTEGEYSSL 119
Cdd:PRK07362  91 LEAIREYGVAIKGPLTT-----PiggGIRSLNVALRQIFDLYSCVRPCRYYAGTPSPHKnpeKLDVIVYRENTEDIYMGI 165

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 120 EHESVAGVVESL----------------------------KIITKAKSLRIAEYAFK--LAQESGRKKVTAVHKANIMKL 169
Cdd:PRK07362 166 EWEAGDEIGDKLikhlneevipaspelgkrqiplgsgigiKPVSKTGSQRHIRRAIEhaLRLPGDKRHVTLVHKGNIMKY 245

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 170 GDGLFLQCCREVAA--------------------RYPQIT-----------FDSM------------------------- 193
Cdd:PRK07362 246 TEGAFRDWGYELATtefrdecvtereswilsnkeKNPNISiednarmiepgYDSLtpekkaaicaevkevldsiwsshgn 325

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564329362 194 ------------IVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFEtATRNTGKSIAN 261
Cdd:PRK07362 326 gkwkekvlvddrIADSIFQQIQTRPQEYSILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE-ATHGTAPKHAG 404

                        330       340       350
                 ....*....|....*....|....*....|....*....
gi 564329362 262 KNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDN 300
Cdd:PRK07362 405 LDRINPGSVILSGVMMLEYLGWQEAADLITKGLSAAIAN 443
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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