N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313  27 PAVRVKTELLESEQGSPNVHNYPDMEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTAS 106
Cdd:cd21441    1 PAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 107 ASSPSSTSTSSSSSSRPASSpTVITSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 186
Cdd:cd21441   81 ASPSSSSSSSSSSSRPASSP-TVITSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 159

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 564387313 187 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 224
Cdd:cd21441  160 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 197

FOG: Zn-finger [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 225 QMEPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQkfaCSISPFSIESTRRQR 304
Cdd:COG5048  233 SQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ---CNISFSRSSPLTRHL 309

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 305 RSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKF-----ARSDELTRHYRKHTGVKPFKC 379
Cdd:COG5048  310 RSVNHSGESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSET 389

                        170       180
                 ....*....|....*....|....
gi 564387313 380 AD--CDRSFSRSDHLALHRRRHML 401
Cdd:COG5048  390 LSnsCIRNFKRDSNLSLHIITHLS 413

N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313  27 PAVRVKTELLESEQGSPNVHNYPDMEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTAS 106
Cdd:cd21441    1 PAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 107 ASSPSSTSTSSSSSSRPASSpTVITSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 186
Cdd:cd21441   81 ASPSSSSSSSSSSSRPASSP-TVITSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 159

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 564387313 187 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 224
Cdd:cd21441  160 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 197

FOG: Zn-finger [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 225 QMEPRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSRVRGNRMNNQkfaCSISPFSIESTRRQR 304
Cdd:COG5048  233 SQLSPKSLLSQSPSSLSSSDSSSSASESPRSSLPTASSQSSSPNESDSSSEKGFSLPIKSKQ---CNISFSRSSPLTRHL 309

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 305 RSESPDSRKRRIHRCDFEGCNKVYTKSSHLKAHRRTHTGEKPYKCTWEGCTWKF-----ARSDELTRHYRKHTGVKPFKC 379
Cdd:COG5048  310 RSVNHSGESLKPFSCPYSLCGKLFSRNDALKRHILLHTSISPAKEKLLNSSSKFspllnNEPPQSLQQYKDLKNDKKSET 389

                        170       180
                 ....*....|....*....|....
gi 564387313 380 AD--CDRSFSRSDHLALHRRRHML 401
Cdd:COG5048  390 LSnsCIRNFKRDSNLSLHIITHLS 413

N-terminal domain of Kruppel-like factor 12; Kruppel-like factor 12 (also known as Krueppel-like transcription factor 12, KLF12) regulates, by transcriptionally repressing Nur77 expression, endometrial decidualization, which is a prerequisite for successful implantation and the establishment of pregnancy. It is involved in the maturation processes of kidney collecting ducts after birth, and is able to increase the promoter activity of the UT-A1 urea transporter promoter by binding to the CACCC motif. KLF12 has also been found to promote colorectal cancer growth is also involved in the invasion and apoptosis of basal-like breast carcinoma. KLF12 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF12 contains an N-terminal domain that is related to the N-terminal repression domain of KLF8.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313  27 PAVRVKTELLESEQGSPNVHNYPDMEAIPLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTAS 106
Cdd:cd21441    1 PAVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKAEPPEDSLSTDHFQTQTEPVDLSINKARTSPTAVSSSPVSMTAS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 107 ASSPSSTSTSSSSSSRPASSpTVITSVSSASSSSTVLSPGPLVASASGMGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 186
Cdd:cd21441   81 ASPSSSSSSSSSSSRPASSP-TVITSVSSASSVPTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQSNKLSHVHRI 159

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 564387313 187 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 224
Cdd:cd21441  160 PVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRSHGKA 197

N-terminal domain of Kruppel-like factor 8; Kruppel-like factor 8 (also known as Krueppel-like transcription factor 8, KLF8) is a CACCC-box binding protein that associates with C-terminal Binding Protein (CtBP) and represses transcription. It plays an essential role in the regulation of the cell cycle, apoptosis, and differentiation. It has been identified as a key component of the transcription factor network that controls terminal differentiation during adipogenesis. It also plays an important role in the formation of several human tumors, including the promotion of tumorigenesis, invasion, and metastasis of colorectal cancer cells, and the progression of pancreatic cancer. KLF8 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Although these factors bind to similar elements in vitro, they have distinct activities in vivo depending on their expression profile and the sequence of the N-terminal activation/repression domain, which differ between members. KLF8 contains an N-terminal repression domain that is related to that of KLF12.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313  55 PLLLNNVKGEPPEDSLPVDHFQTQTEPVDLSINKARTSPTAASSSPVSMTASASSPSSTSTSSSSSSRPASSPTVItsvs 134
Cdd:cd21440   13 PALLSDIKTEPPEELLASDCSQPQAEPVDLSLHKPKAPLQPPSVLSPSPMILSVSPSAPQSLVSSTGTGMGTTSAI---- 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564387313 135 sasssSTVLSPGPLVASASGMGGQQFLHIIHPVPpssPMNLQSnKLSHVHRIPVVVQSVPVVYTAVRSPGnVNNTIVVPL 214
Cdd:cd21440   89 -----PAVLSPGSILASSQGSGGQQILHVIHTIP---SVNLPS-KMGNLQTIPVVVQSLPVVYTTLPTDG-VTAAITVPL 158

                 ....*....
gi 564387313 215 L-EDGRSHG 222
Cdd:cd21440  159 IgGDGKNAG 167

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 564387313  377 FKCADCDRSFSRSDHLALHRRRH 399
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

N-terminal domain of Kruppel-like factor 3; Kruppel-like factor 3 (KLF3; also called Krueppel-like factor 3 and originally called Basic Kruppel-like Factor/BKLF), was the third member of the KLF family of zinc finger transcription factors to be discovered. KLF3 possesses a wide range of biological impacts on regulating apoptosis, differentiation, and proliferation in various tissues during the entire progression process. It has been proposed as a tumor suppressor in colorectal cancer. It appears to function predominantly as a repressor of transcription, turning genes off by recruiting the C-terminal Binding Protein co-repressors CtBP1 and CtBP2. CtBP docks onto a short motif (residues 61-65) in the N-terminus of KLF3, through the Proline-X-Aspartate-Leucine-Serine (PXDLS) motif. CtBP in turn recruits histone modifying enzymes to alter chromatin and repress gene expression. KLF3 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF3.

                         10        20        30
                 ....*....|....*....|....*....|....*
gi 564387313  66 PEDSLPVDHfQTQTEPVDLSINKaRTSPTAASSSP 100
Cdd:cd21577    7 METSFYSPS-HSQLEPVDLSLSK-RSSPPSSSSSS 39

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|....*
gi 564387313  317 HRCdfEGCNKVYTKSSHLKAHRRTH 341
Cdd:pfam00096   1 YKC--PDCGKSFSRKSNLKRHLRTH 23