Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor; SAM Pointed domain of PDEF-like (Prostate-Derived ETS Factor) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. In human males this activator is highly expressed in the prostate gland and enhances androgen-mediated activation of the PSA promoter though interaction with the DNA binding domain of androgen receptor. PDEF may play a role in prostate cancer development as well as in goblet cell formation and mucus production in the epithelial lining of respiratory and intestinal tracts.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509 124 IETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPP-AGKAFQElGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWKSA 202
Cdd:cd08532    1 IDTTCALLGISPDPYQWSTANVQAWLQWTEHQYRLPPnPPRCFQM-NGKTLCALSEEDFVRRAPQGGDTLHAQLEIWKSA 79

                 ..
gi 569000509 203 AW 204
Cdd:cd08532   80 AE 81

Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor; SAM Pointed domain of PDEF-like (Prostate-Derived ETS Factor) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. In human males this activator is highly expressed in the prostate gland and enhances androgen-mediated activation of the PSA promoter though interaction with the DNA binding domain of androgen receptor. PDEF may play a role in prostate cancer development as well as in goblet cell formation and mucus production in the epithelial lining of respiratory and intestinal tracts.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509 124 IETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPP-AGKAFQElGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWKSA 202
Cdd:cd08532    1 IDTTCALLGISPDPYQWSTANVQAWLQWTEHQYRLPPnPPRCFQM-NGKTLCALSEEDFVRRAPQGGDTLHAQLEIWKSA 79

                 ..
gi 569000509 203 AW 204
Cdd:cd08532   80 AE 81

Ets-domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509  240 HLWQFLKELLLKPhSYGRFIRWLNKEKGIFKIEDSAQVARLWGVRKNRPAMNYDKLSRSIRQYYKKGIIRKPDiSQRLVY 319
Cdd:pfam00178   1 QLWQFLLDLLTDP-EYSDIIKWTDKEEGEFRLVDPEAVARLWGKRKGNPKMTYEKLSRALRYYYKKGILEKVP-GKRLTY 78

                  ..
gi 569000509  320 QF 321
Cdd:pfam00178  79 RF 80

Sterile alpha motif (SAM)/Pointed domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509  121 LKDIETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWK 200
Cdd:pfam02198   1 LPSFEKEQLRLWIPADPRLWTKDHVLEWLEWAVDEFDLSKIDFSQFDMNGKALCSLGKEEFLQRAPGPGDILWSHLQILR 80

                  ...
gi 569000509  201 SAA 203
Cdd:pfam02198  81 KAS 83

Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor; SAM Pointed domain of PDEF-like (Prostate-Derived ETS Factor) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. In human males this activator is highly expressed in the prostate gland and enhances androgen-mediated activation of the PSA promoter though interaction with the DNA binding domain of androgen receptor. PDEF may play a role in prostate cancer development as well as in goblet cell formation and mucus production in the epithelial lining of respiratory and intestinal tracts.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509 124 IETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPP-AGKAFQElGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWKSA 202
Cdd:cd08532    1 IDTTCALLGISPDPYQWSTANVQAWLQWTEHQYRLPPnPPRCFQM-NGKTLCALSEEDFVRRAPQGGDTLHAQLEIWKSA 79

                 ..
gi 569000509 203 AW 204
Cdd:cd08532   80 AE 81

Ets-domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509  240 HLWQFLKELLLKPhSYGRFIRWLNKEKGIFKIEDSAQVARLWGVRKNRPAMNYDKLSRSIRQYYKKGIIRKPDiSQRLVY 319
Cdd:pfam00178   1 QLWQFLLDLLTDP-EYSDIIKWTDKEEGEFRLVDPEAVARLWGKRKGNPKMTYEKLSRALRYYYKKGILEKVP-GKRLTY 78

                  ..
gi 569000509  320 QF 321
Cdd:pfam00178  79 RF 80

Sterile alpha motif (SAM)/Pointed domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509  121 LKDIETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAPLGGDVLHAHLDIWK 200
Cdd:pfam02198   1 LPSFEKEQLRLWIPADPRLWTKDHVLEWLEWAVDEFDLSKIDFSQFDMNGKALCSLGKEEFLQRAPGPGDILWSHLQILR 80

                  ...
gi 569000509  201 SAA 203
Cdd:pfam02198  81 KAS 83

Sterile alpha motif (SAM)/Pointed domain of ERG transcription factor; SAM Pointed domain of ERG subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. It may participate in formation of homodimers or heterodimers with ETS-2, Fli-1, ER81, and Pu-1. However, dimeric forms are inactive and SAM Pointed domain is not essential for dimerization, since ER81 and Pu-1 do not have it. In mouse, a regulator of this type binds the ESET histone H3-specific methyltransferase (human homolog is SETDB1), which leads to modification of the local chromatin structure through histone methylation. ERG regulators are involved in endothelial cell differentiation, bone morphogenesis and neural crest development. The Erg gene is a proto-oncogene. It is a target of chromosomal translocations resulting in fusions with other neighboring genes. Chimeric proteins were found in solid tumors such as myeloid leukemia or Ewing's sarcoma. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569000509 133 ITADPGDWSPGNVQKWLLWTEHQYRLPPAGKA-FQELGGKELCAMSEEQFRQRAP-LGGDVLHAHLDiwksaaWMKERTS 210
Cdd:cd08540    1 VPADPTLWSTDHVRQWLEWAVKEYGLPDVDVLlFQNIDGKELCKMTKEDFQRLTPsYNADILLSHLH------YLRETPL 74

                 .
gi 569000509 211 P 211
Cdd:cd08540   75 P 75

Sterile alpha motif (SAM)/Pointed domain of GA-binding protein (GABP) alpha chain; SAM Pointed domain of GA-binding protein (GABP) alpha subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. This type of transcriptional regulators forms heterotetramers containing two alpha and two beta subunits. It interacts with GA repeats (purine rich repeats). GABP transcriptional factors control gene expression in cell cycle control, apoptosis, and cellular respiration. GABP participates in regulation of transmembrane receptors and key hormones especially in myeloid cells and at the neuromuscular junction.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 569000509 131 LNITADPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAPLG-GDVLHAHLDIWK 200
Cdd:cd08534   13 LKIPYDPMEWTEDHVLHWLQWAVKEFGLTGINLSDWNITGRELCSLTQEEFLQRVPKDpGDIFWTHLELLR 83

Sterile alpha motif (SAM)/Pointed domain of Mae protein homolog; Mae (Modulator of the Activity of ETS) subfamily represents a group of SAM Pointed monodomain proteins. SAM Pointed domain is a protein-protein interaction domain. It can interact with other SAM pointed domains forming head-to-tail heterodimers and also provides a kinase docking site. For example, in Drosophila Mae is required for facilitating phosphorylation of the Yan factor and for blocking phosphorylation of the ETS-2 regulator. Mae interacts with the SAM Pointed domains of Yan and ETS-2. Binding enhances access of the kinase to the Yan phosphorylation site by providing a kinase docking site, or inhibits phosphorylation of ETS-2 by blocking its docking site. This type of factors participates in regulation of kinase signaling particularly during embryogenesis.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 569000509 136 DPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAPLGGDVLhaHLDIWK 200
Cdd:cd08536    1 DPRSWSREHVRTWLRWVSARYQLEVVDLDKFLMNGKGLCLMSLEGFLYRVPVGGKLL--YEDFQR 63

Sterile alpha motif (SAM)/Pointed domain of ESE-like ETS transcriptional regulators; SAM Pointed domain of ESE-like (Epithelium-Specific ETS) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. It can act as a major transactivator by providing a potential docking site for co-activators. ETS factors are important for cell differentiation. They can be involved in regulation of gene expression in different types of epithelial cells. They are expressed in salivary gland, intestine, stomach, pancreas, lungs, kidneys, colon, mammary gland, and prostate. Members of this group are proto-oncogenes. Expression profiles of these factors are altered in epithelial cancers, which makes them potential targets for cancer therapy.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 569000509 136 DPGDWSPGNVQKWLLWTEHQYRLPPA---GKAFQELGGKELCAMSEEQFRQRAPLGGDVLHAHL 196
Cdd:cd08757    1 HPQHWTKNDVLDWLLFVAEQNKIDAEeinFQKFDNIDGQTLCSMSLEEFIERDGVYGNLLYAEL 64

Sterile alpha motif (SAM)/Pointed domain of ETS-1,2 family; SAM Pointed domain of ETS-1,2 family of transcriptional activators is a protein-protein interaction domain. It carries a kinase docking site and mediates interaction between ETS transcriptional activators and protein kinases. This group of transcriptional factors is involved in the Ras/MAP kinase signaling pathway. MAP kinases phosphorylate the transcription factors. Phosphorylated factors then recruit coactivators and enhance transactivation. Members of this group play a role in regulation of different embryonic developmental processes. ETS-1,2 transcriptional activators are proto-oncogenes involved in malignant transformation and tumor progression. They are potential molecular targets for selective cancer therapy.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 569000509 134 TADPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQELGGKELCAMSEEQFRQRAP-LGGDVLHAHLDI 198
Cdd:cd08533    1 PKDPRQWSETQVAYWLDWAANEFSLEGVNLSNFCMSGRDMCALGKEAFLSLAPpFVGDILWEHLDI 66

Sterile alpha motif (SAM)/Pointed domain of ESE-2 like ETS transcriptional regulators; SAM Pointed domain of ESE-2-like (Epithelium-Specific ETS) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. It can act as a major transactivator by providing a potential docking site for co-activators. ESE-2 factors are involved in regulation of gene expression in a variety of epithelial (glandular and secretory) cells. ESE-2 mRNA was found in skin keratinocytes, salivary gland, mammary gland, stomach, prostate, and kidneys. The DNA binding consensus motif for ESE-2 consists of a GGA core and AT-rich flanks. The expression profiles of these factors are altered in epithelial cancers. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 569000509 125 ETACKLLNITADPGDWSPGNVQKWLLWTEHQYRLPPAGKAFQE--LGGKELCAMSEEQFRQRAPLGGDVLH 193
Cdd:cd08538    1 QTACDSYWTSVHPEYWTKRHVWEWLQFCCDQYKLDANCISFCHfnISGLQLCSMTQEEFLDAAGICGEYLY 71