NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|767957705|ref|XP_011517090|]
View 

sialic acid synthase isoform X2 [Homo sapiens]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
SpsE super family cl30261
Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope ...
 23-226 9.15e-80

Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope biogenesis];


The actual alignment was detected with superfamily member COG2089:

Pssm-ID: 441692 [Multi-domain]  Cd Length: 335  Bit Score: 242.31  E-value: 9.15e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705  23 KKGRPMVISSGMQSMDTMKQVYQIVKPL-NPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFpDIPIGYSGHETGIAISVA 101
Cdd:COG2089  133 KTGKPIILSTGMATLEEIEEAVEALREAgNDQIALLHCTSAYPAPPEDVNLRAIPTLKEAF-GVPVGLSDHTLGIEVPIA 211

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705 102 AVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTI 181
Cdd:COG2089  212 AVALGASVIEKHFTLDRSLPGPDHAFSLEPDELKAMVEAIRNAEKALGSGIKGPTPSEKKNRRVARRSLVAARDIKAGEV 291

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 767957705 182 LTMDMLTVKvgEP-KGYPPEDIFNLVGKKVLVTVEEDDTIMEELVD 226
Cdd:COG2089  292 ITEENLRVK--RPgTGLSPKYLDEVLGKKAKRDIKAGTPLTWDDLE 335
 
Name Accession Description Interval E-value
SpsE COG2089
Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope ...
 23-226 9.15e-80

Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441692 [Multi-domain]  Cd Length: 335  Bit Score: 242.31  E-value: 9.15e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705  23 KKGRPMVISSGMQSMDTMKQVYQIVKPL-NPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFpDIPIGYSGHETGIAISVA 101
Cdd:COG2089  133 KTGKPIILSTGMATLEEIEEAVEALREAgNDQIALLHCTSAYPAPPEDVNLRAIPTLKEAF-GVPVGLSDHTLGIEVPIA 211

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705 102 AVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTI 181
Cdd:COG2089  212 AVALGASVIEKHFTLDRSLPGPDHAFSLEPDELKAMVEAIRNAEKALGSGIKGPTPSEKKNRRVARRSLVAARDIKAGEV 291

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 767957705 182 LTMDMLTVKvgEP-KGYPPEDIFNLVGKKVLVTVEEDDTIMEELVD 226
Cdd:COG2089  292 ITEENLRVK--RPgTGLSPKYLDEVLGKKAKRDIKAGTPLTWDDLE 335
NeuB pfam03102
NeuB family; NeuB is the prokaryotic N-acetylneuraminic acid (Neu5Ac) synthase. It catalyzes ...
 23-153 7.51e-59

NeuB family; NeuB is the prokaryotic N-acetylneuraminic acid (Neu5Ac) synthase. It catalyzes the direct formation of Neu5Ac (the most common sialic acid) by condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine (ManNAc). This reaction has only been observed in prokaryotes; eukaryotes synthesize the 9-phosphate form, Neu5Ac-9-P, and utilize ManNAc-6-P instead of ManNAc. Such eukaryotic enzymes are not present in this family. This family also contains SpsE spore coat polysaccharide biosynthesis proteins.


Pssm-ID: 427137  Cd Length: 239  Bit Score: 185.79  E-value: 7.51e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705   23 KKGRPMVISSGMQSMDTMKQVYQIV-KPLNPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFPdIPIGYSGHETGIAISVA 101
Cdd:pfam03102 109 KTGKPVILSTGMATLGEIEEAVEVLrRAGNEDLTLLHCTSEYPAPFEDVNLRAIPTLKEAFG-VPVGYSDHTLGIAAPIA 187

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 767957705  102 AVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTK 153
Cdd:pfam03102 188 AVALGAKVIEKHFTLDRNLPGPDHAASLEPDELKEMVRAIRNVEKALGDGIK 239
SAF_NeuB_like cd11615
C-terminal SAF domain of sialic acid synthetase; Sialic acid synthetase (N-acetylneuraminate ...
 167-225 1.88e-12

C-terminal SAF domain of sialic acid synthetase; Sialic acid synthetase (N-acetylneuraminate synthase or N-acetylneuraminate-9-phosphate synthase) catalyzes the condensation of phosphoenolpyruvate with N-acetylmannosamine (ManNAc, in bacteria) or N-acetylmannosamine-6-phosphate (ManNAc-6P, in mammals), to yield N-acetylneuramic acid (NeuNAc) or N-acetylneuramic acid-9-phosphate (NeuNAc-9P), respectively. The N-terminal NeuB domain, a TIM-barrel-like structure, contains the catalytic site, the function of the SAF domain is not as clear. It may participate in domain-swapped dimerization and play a role in binding the substrate, in either domain-swapped dimers or by directly interacting with the N-terminal domain. Also included in the family are PEP-sugar pyruvyltransferases known as spore coat polysaccharide biosynthesis proteins (SpsE).


Pssm-ID: 212160 [Multi-domain]  Cd Length: 58  Bit Score: 60.05  E-value: 1.88e-12
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 767957705 167 GKSVVAKVKIPEGTILTMDMLTVKVGEpKGYPPEDIFNLVGKKVLVTVEEDDTIMEELV 225
Cdd:cd11615    1 RRSLVAARDIKAGEVLTEENLRVKRPG-GGLSPKYLDEVLGKKAKRDIKAGEPLTWDDL 58
SAF smart00858
This domain family includes a range of different proteins. Such as antifreeze proteins and ...
 168-225 2.60e-04

This domain family includes a range of different proteins. Such as antifreeze proteins and flagellar FlgA proteins, and CpaB pilus proteins;


Pssm-ID: 214862 [Multi-domain]  Cd Length: 63  Bit Score: 37.93  E-value: 2.60e-04
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 767957705   168 KSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPE---DIFNLVGKKVLVTVEEDDTIMEELV 225
Cdd:smart00858   2 NVVVAARDLPAGEVITAEDLRLGHVALRDLPGGgltPYGQVIGRVARRDIAAGEPITASNL 62
PRK08195 PRK08195
4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase,; Validated
 61-110 1.25e-03

4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase,; Validated


Pssm-ID: 181282 [Multi-domain]  Cd Length: 337  Bit Score: 39.05  E-value: 1.25e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 767957705  61 SAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHET---GIAISVAAVALGAKVL 110
Cdd:PRK08195 166 SAGALLPEDVRDRVRALRAALKPDTQVGFHGHNNlglGVANSLAAVEAGATRI 218
 
Name Accession Description Interval E-value
SpsE COG2089
Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope ...
 23-226 9.15e-80

Sialic acid synthase SpsE, contains C-terminal SAF domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441692 [Multi-domain]  Cd Length: 335  Bit Score: 242.31  E-value: 9.15e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705  23 KKGRPMVISSGMQSMDTMKQVYQIVKPL-NPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFpDIPIGYSGHETGIAISVA 101
Cdd:COG2089  133 KTGKPIILSTGMATLEEIEEAVEALREAgNDQIALLHCTSAYPAPPEDVNLRAIPTLKEAF-GVPVGLSDHTLGIEVPIA 211

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705 102 AVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTKQLLPCEMACNEKLGKSVVAKVKIPEGTI 181
Cdd:COG2089  212 AVALGASVIEKHFTLDRSLPGPDHAFSLEPDELKAMVEAIRNAEKALGSGIKGPTPSEKKNRRVARRSLVAARDIKAGEV 291

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 767957705 182 LTMDMLTVKvgEP-KGYPPEDIFNLVGKKVLVTVEEDDTIMEELVD 226
Cdd:COG2089  292 ITEENLRVK--RPgTGLSPKYLDEVLGKKAKRDIKAGTPLTWDDLE 335
NeuB pfam03102
NeuB family; NeuB is the prokaryotic N-acetylneuraminic acid (Neu5Ac) synthase. It catalyzes ...
 23-153 7.51e-59

NeuB family; NeuB is the prokaryotic N-acetylneuraminic acid (Neu5Ac) synthase. It catalyzes the direct formation of Neu5Ac (the most common sialic acid) by condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine (ManNAc). This reaction has only been observed in prokaryotes; eukaryotes synthesize the 9-phosphate form, Neu5Ac-9-P, and utilize ManNAc-6-P instead of ManNAc. Such eukaryotic enzymes are not present in this family. This family also contains SpsE spore coat polysaccharide biosynthesis proteins.


Pssm-ID: 427137  Cd Length: 239  Bit Score: 185.79  E-value: 7.51e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705   23 KKGRPMVISSGMQSMDTMKQVYQIV-KPLNPNFCFLQCTSAYPLQPEDVNLRVISEYQKLFPdIPIGYSGHETGIAISVA 101
Cdd:pfam03102 109 KTGKPVILSTGMATLGEIEEAVEVLrRAGNEDLTLLHCTSEYPAPFEDVNLRAIPTLKEAFG-VPVGYSDHTLGIAAPIA 187

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 767957705  102 AVALGAKVLERHITLDKTWKGSDHSASLEPGELAELVRSVRLVERALGSPTK 153
Cdd:pfam03102 188 AVALGAKVIEKHFTLDRNLPGPDHAASLEPDELKEMVRAIRNVEKALGDGIK 239
SAF_NeuB_like cd11615
C-terminal SAF domain of sialic acid synthetase; Sialic acid synthetase (N-acetylneuraminate ...
 167-225 1.88e-12

C-terminal SAF domain of sialic acid synthetase; Sialic acid synthetase (N-acetylneuraminate synthase or N-acetylneuraminate-9-phosphate synthase) catalyzes the condensation of phosphoenolpyruvate with N-acetylmannosamine (ManNAc, in bacteria) or N-acetylmannosamine-6-phosphate (ManNAc-6P, in mammals), to yield N-acetylneuramic acid (NeuNAc) or N-acetylneuramic acid-9-phosphate (NeuNAc-9P), respectively. The N-terminal NeuB domain, a TIM-barrel-like structure, contains the catalytic site, the function of the SAF domain is not as clear. It may participate in domain-swapped dimerization and play a role in binding the substrate, in either domain-swapped dimers or by directly interacting with the N-terminal domain. Also included in the family are PEP-sugar pyruvyltransferases known as spore coat polysaccharide biosynthesis proteins (SpsE).


Pssm-ID: 212160 [Multi-domain]  Cd Length: 58  Bit Score: 60.05  E-value: 1.88e-12
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 767957705 167 GKSVVAKVKIPEGTILTMDMLTVKVGEpKGYPPEDIFNLVGKKVLVTVEEDDTIMEELV 225
Cdd:cd11615    1 RRSLVAARDIKAGEVLTEENLRVKRPG-GGLSPKYLDEVLGKKAKRDIKAGEPLTWDDL 58
Antifreeze_III cd11617
Type III antifreeze protein, may be specific to the Zoarcoidei; Antifreeze protein III ...
 168-228 5.47e-09

Type III antifreeze protein, may be specific to the Zoarcoidei; Antifreeze protein III inhibits the growth of ice crystals and protects fish from cold damage in sub-freezing temperatures.


Pssm-ID: 212162  Cd Length: 62  Bit Score: 50.93  E-value: 5.47e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 767957705 168 KSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELVDNH 228
Cdd:cd11617    1 ASVVATQLIPINTALTLVMMKAKVVTPMGIPAEEIPRLVGMQVNRAVPLGTTLMPDMVKGY 61
CpaB COG3745
Flp pilus assembly protein CpaB [Intracellular trafficking, secretion, and vesicular transport, ...
 170-223 4.86e-06

Flp pilus assembly protein CpaB [Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 442959 [Multi-domain]  Cd Length: 259  Bit Score: 46.13  E-value: 4.86e-06
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 767957705 170 VVAKVKIPEGTILTMDMLTVK-VgePKGYPPEDIFN----LVGKKVLVTVEEDDTIMEE 223
Cdd:COG3745   44 VVAARDIPAGTPLTADDLAVVeW--PADAVPEGAFTdpeeLVGRVARVPIEAGEPILAS 100
SAF pfam08666
SAF domain; This domain family includes a range of different proteins. Such as antifreeze ...
 169-225 2.46e-05

SAF domain; This domain family includes a range of different proteins. Such as antifreeze proteins and flagellar FlgA proteins, and CpaB pilus proteins.


Pssm-ID: 430140 [Multi-domain]  Cd Length: 63  Bit Score: 41.00  E-value: 2.46e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705  169 SVVAKVKIPEGTILTMDMLTVK---VGEPKGYPPEDIFNLVGKKVLVTVEEDDTIMEELV 225
Cdd:pfam08666   3 VVVAARDLPAGEVITADDLTLVrppLALPPGLFPIAYGEVIGKVARRDIAAGEPLTASDL 62
SAF smart00858
This domain family includes a range of different proteins. Such as antifreeze proteins and ...
 168-225 2.60e-04

This domain family includes a range of different proteins. Such as antifreeze proteins and flagellar FlgA proteins, and CpaB pilus proteins;


Pssm-ID: 214862 [Multi-domain]  Cd Length: 63  Bit Score: 37.93  E-value: 2.60e-04
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 767957705   168 KSVVAKVKIPEGTILTMDMLTVKVGEPKGYPPE---DIFNLVGKKVLVTVEEDDTIMEELV 225
Cdd:smart00858   2 NVVVAARDLPAGEVITAEDLRLGHVALRDLPGGgltPYGQVIGRVARRDIAAGEPITASNL 62
SAF_CpaB_FlgA_like cd11614
SAF domains of the flagella basal body P-ring formation protein FlgA and the flp pilus ...
 170-225 4.61e-04

SAF domains of the flagella basal body P-ring formation protein FlgA and the flp pilus assembly CpaB; FlgA is a putative periplasmic chaperone that assists in the formation of the flagellar P ring; CpaB is a protein invoved in the assembly of the flp pili, which are bacterial virulence factors mediating non-specific adherence to surfaces; these proteins appear to contain a single SAF domain. This intermediate family also contains the SAF domains of sialic acid synthetases and type III antifreeze proteins, which also share the same extensive core structure.


Pssm-ID: 212159 [Multi-domain]  Cd Length: 61  Bit Score: 37.45  E-value: 4.61e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 767957705 170 VVAKVKIPEGTILTMDMLTVKVGePKGYPPEDIF----NLVGKKVLVTVEEDDTIMEELV 225
Cdd:cd11614    3 VVAARDLPAGTVITADDLTLVEV-PLSLLPPGALtdpdDVVGRVARRPLRAGEPITASML 61
PRK08195 PRK08195
4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase,; Validated
 61-110 1.25e-03

4-hyroxy-2-oxovalerate/4-hydroxy-2-oxopentanoic acid aldolase,; Validated


Pssm-ID: 181282 [Multi-domain]  Cd Length: 337  Bit Score: 39.05  E-value: 1.25e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 767957705  61 SAYPLQPEDVNLRVISEYQKLFPDIPIGYSGHET---GIAISVAAVALGAKVL 110
Cdd:PRK08195 166 SAGALLPEDVRDRVRALRAALKPDTQVGFHGHNNlglGVANSLAAVEAGATRI 218
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH