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Conserved domains on  [gi|767942436|ref|XP_011533963|]
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AFG1-like ATPase isoform X8 [Homo sapiens]

Protein Classification

AFG1/ZapE family ATPase( domain architecture ID 1903377)

AFG1/ZapE family ATPase similar to cell division protein ZapE, that interacts with FtsZ and modulates the Z-ring dynamics

EC:  3.6.-.-
Gene Ontology:  GO:0016887|GO:0005524
PubMed:  1441755|24595368

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ZapE super family cl43431
Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, ...
 73-250 6.25e-84

Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, cell division, chromosome partitioning];


The actual alignment was detected with superfamily member COG1485:

Pssm-ID: 441094  Cd Length: 365  Bit Score: 254.64  E-value: 6.25e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  73 HGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSK---PPRGLYVYGDVGTGKTMVMDMF 149
Cdd:COG1485    3 MSPLERYQALLAAGGLQPDPAQEAAVARLDRLYDELLAYRPRKSGLLGRLFGRKRkpePPKGLYLWGGVGRGKTMLMDLF 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 150 YAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSlpkrkpgfmaksYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQL 229
Cdd:COG1485   83 FESLPVERKRRVHFHRFMQEVHARLHALRGQ------------ADPLPPVADRIAAEARLLCFDEFHVTDIADAMILGRL 150

                        170       180
                 ....*....|....*....|.
gi 767942436 230 FENLFKNGVVVVATSNRPPEG 250
Cdd:COG1485  151 FEALFARGVVLVATSNIPPDD 171
 
Name Accession Description Interval E-value
ZapE COG1485
Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, ...
 73-250 6.25e-84

Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 441094  Cd Length: 365  Bit Score: 254.64  E-value: 6.25e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  73 HGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSK---PPRGLYVYGDVGTGKTMVMDMF 149
Cdd:COG1485    3 MSPLERYQALLAAGGLQPDPAQEAAVARLDRLYDELLAYRPRKSGLLGRLFGRKRkpePPKGLYLWGGVGRGKTMLMDLF 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 150 YAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSlpkrkpgfmaksYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQL 229
Cdd:COG1485   83 FESLPVERKRRVHFHRFMQEVHARLHALRGQ------------ADPLPPVADRIAAEARLLCFDEFHVTDIADAMILGRL 150

                        170       180
                 ....*....|....*....|.
gi 767942436 230 FENLFKNGVVVVATSNRPPEG 250
Cdd:COG1485  151 FEALFARGVVLVATSNIPPDD 171
ZapE NF040713
cell division protein ZapE;
78-250 3.02e-71

cell division protein ZapE;


Pssm-ID: 468677 [Multi-domain]  Cd Length: 344  Bit Score: 221.62  E-value: 3.02e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  78 HYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAeGLFSKLFSRSK-PPRGLYVYGDVGTGKTMVMDMFYAYVEMK 156
Cdd:NF040713   1 RYEAAVAFGGYRPDPAQAAAVERLDRLAEELAAPAKKR-GLLGRLFGRKKsPPRGLYLWGGVGRGKTMLMDLFYEALPIE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 157 RKKRVHFHGFMLDVHKRIHRLKqslpkrkpGFMaksyDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKN 236
Cdd:NF040713  80 RKRRVHFHEFMREVHQRLALLR--------GQA----DPLPPVADEIASEARLLCFDEFHVDDIADAMILGRLFEALFER 147

                        170
                 ....*....|....
gi 767942436 237 GVVVVATSNRPPEG 250
Cdd:NF040713 148 GVVLVATSNTPPDD 161
AFG1_ATPase pfam03969
AFG1-like ATPase; This P-loop motif-containing family of proteins includes AFG1, LACE1 and ...
 91-250 4.36e-45

AFG1-like ATPase; This P-loop motif-containing family of proteins includes AFG1, LACE1 and ZapE. ATPase family gene 1 (AFG1) is a 377 amino acid yeast protein with an ATPase motif typical of the family. LACE1, the mammalian homolog of AFG1, is a mitochondrial integral membrane protein that is essential for maintenance of fused mitochondrial reticulum and lamellar cristae morphology. It has also been demonstrated that LACE1 mediates degradation of nuclear-encoded complex IV subunits COX4 (cytochrome c oxidase 4), COX5A and COX6A, and is required for normal activity of complexes III and IV of the respiratory chain. ZapE is a cell division protein found in Gram-negative bacteria. The bacterial cell division process relies on the assembly, positioning, and constriction of FtsZ ring (the so-called Z-ring), a ring-like network that marks the future site of the septum of bacterial cell division. ZapE is a Z-ring associated protein required for cell division under low-oxygen conditions. It is an ATPase that appears at the constricting Z-ring late in cell division. It reduces the stability of FtsZ polymers in the presence of ATP in vitro.


Pssm-ID: 397872  Cd Length: 361  Bit Score: 154.97  E-value: 4.36e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436   91 DEHQRRVIQCLQKLHEDL-KGYNIEAEGLFSKLFSRSK---PPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKKRVHFHGF 166
Cdd:pfam03969  19 DVAQANAVPHLDRLYQDLaAQDFPRPARAGGKLWGRKPsvqPPRGLYLWGGVGRGKTWLMDLFFESLPGEKKRRTHFHRF 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  167 MLDVHKRIHRLKqslpkrkpgfmaKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNR 246
Cdd:pfam03969  99 MFRVHDELTTLQ------------GGVDPLAIIADRLAGEARLLCFDEFFVSDIGDAMLLGRLFEALFARGVTLVATSNI 166


                  ....
gi 767942436  247 PPEG 250
Cdd:pfam03969 167 APEQ 170
AAA cd00009
The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily ...
 121-251 5.08e-03

The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, RecA-like, VirD4-like, PilT-like, and SF1/2 helicases. Members of the AAA+ ATPases function as molecular chaperons, ATPase subunits of proteases, helicases, or nucleic-acid stimulated ATPases. The AAA+ proteins contain several distinct features in addition to the conserved alpha-beta-alpha core domain structure and the Walker A and B motifs of the P-loop NTPases.


Pssm-ID: 99707 [Multi-domain]  Cd Length: 151  Bit Score: 36.36  E-value: 5.08e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 121 KLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKK--RVHFHGFMLDvhKRIHRLKQSLPKRKPGFMAKSYDPiap 198
Cdd:cd00009   11 REALELPPPKNLLLYGPPGTGKTTLARAIANELFRPGAPflYLNASDLLEG--LVVAELFGHFLVRLLFELAEKAKP--- 85

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 767942436 199 iaeeiseeaCLLCFDEFQ-VTDIADAMILKQL----FENLFKNGVVVVATSNRPPEGP 251
Cdd:cd00009   86 ---------GVLFIDEIDsLSRGAQNALLRVLetlnDLRIDRENVRVIGATNRPLLGD 134
 
Name Accession Description Interval E-value
ZapE COG1485
Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, ...
 73-250 6.25e-84

Cell division protein ZapE (Z ring-associated ATPase), AFG1 superfamily [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 441094  Cd Length: 365  Bit Score: 254.64  E-value: 6.25e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  73 HGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSK---PPRGLYVYGDVGTGKTMVMDMF 149
Cdd:COG1485    3 MSPLERYQALLAAGGLQPDPAQEAAVARLDRLYDELLAYRPRKSGLLGRLFGRKRkpePPKGLYLWGGVGRGKTMLMDLF 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 150 YAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSlpkrkpgfmaksYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQL 229
Cdd:COG1485   83 FESLPVERKRRVHFHRFMQEVHARLHALRGQ------------ADPLPPVADRIAAEARLLCFDEFHVTDIADAMILGRL 150

                        170       180
                 ....*....|....*....|.
gi 767942436 230 FENLFKNGVVVVATSNRPPEG 250
Cdd:COG1485  151 FEALFARGVVLVATSNIPPDD 171
ZapE NF040713
cell division protein ZapE;
78-250 3.02e-71

cell division protein ZapE;


Pssm-ID: 468677 [Multi-domain]  Cd Length: 344  Bit Score: 221.62  E-value: 3.02e-71
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  78 HYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAeGLFSKLFSRSK-PPRGLYVYGDVGTGKTMVMDMFYAYVEMK 156
Cdd:NF040713   1 RYEAAVAFGGYRPDPAQAAAVERLDRLAEELAAPAKKR-GLLGRLFGRKKsPPRGLYLWGGVGRGKTMLMDLFYEALPIE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 157 RKKRVHFHGFMLDVHKRIHRLKqslpkrkpGFMaksyDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKN 236
Cdd:NF040713  80 RKRRVHFHEFMREVHQRLALLR--------GQA----DPLPPVADEIASEARLLCFDEFHVDDIADAMILGRLFEALFER 147

                        170
                 ....*....|....
gi 767942436 237 GVVVVATSNRPPEG 250
Cdd:NF040713 148 GVVLVATSNTPPDD 161
AFG1_ATPase pfam03969
AFG1-like ATPase; This P-loop motif-containing family of proteins includes AFG1, LACE1 and ...
 91-250 4.36e-45

AFG1-like ATPase; This P-loop motif-containing family of proteins includes AFG1, LACE1 and ZapE. ATPase family gene 1 (AFG1) is a 377 amino acid yeast protein with an ATPase motif typical of the family. LACE1, the mammalian homolog of AFG1, is a mitochondrial integral membrane protein that is essential for maintenance of fused mitochondrial reticulum and lamellar cristae morphology. It has also been demonstrated that LACE1 mediates degradation of nuclear-encoded complex IV subunits COX4 (cytochrome c oxidase 4), COX5A and COX6A, and is required for normal activity of complexes III and IV of the respiratory chain. ZapE is a cell division protein found in Gram-negative bacteria. The bacterial cell division process relies on the assembly, positioning, and constriction of FtsZ ring (the so-called Z-ring), a ring-like network that marks the future site of the septum of bacterial cell division. ZapE is a Z-ring associated protein required for cell division under low-oxygen conditions. It is an ATPase that appears at the constricting Z-ring late in cell division. It reduces the stability of FtsZ polymers in the presence of ATP in vitro.


Pssm-ID: 397872  Cd Length: 361  Bit Score: 154.97  E-value: 4.36e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436   91 DEHQRRVIQCLQKLHEDL-KGYNIEAEGLFSKLFSRSK---PPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKKRVHFHGF 166
Cdd:pfam03969  19 DVAQANAVPHLDRLYQDLaAQDFPRPARAGGKLWGRKPsvqPPRGLYLWGGVGRGKTWLMDLFFESLPGEKKRRTHFHRF 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436  167 MLDVHKRIHRLKqslpkrkpgfmaKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNR 246
Cdd:pfam03969  99 MFRVHDELTTLQ------------GGVDPLAIIADRLAGEARLLCFDEFFVSDIGDAMLLGRLFEALFARGVTLVATSNI 166


                  ....
gi 767942436  247 PPEG 250
Cdd:pfam03969 167 APEQ 170
AAA cd00009
The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily ...
 121-251 5.08e-03

The AAA+ (ATPases Associated with a wide variety of cellular Activities) superfamily represents an ancient group of ATPases belonging to the ASCE (for additional strand, catalytic E) division of the P-loop NTPase fold. The ASCE division also includes ABC, RecA-like, VirD4-like, PilT-like, and SF1/2 helicases. Members of the AAA+ ATPases function as molecular chaperons, ATPase subunits of proteases, helicases, or nucleic-acid stimulated ATPases. The AAA+ proteins contain several distinct features in addition to the conserved alpha-beta-alpha core domain structure and the Walker A and B motifs of the P-loop NTPases.


Pssm-ID: 99707 [Multi-domain]  Cd Length: 151  Bit Score: 36.36  E-value: 5.08e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767942436 121 KLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKK--RVHFHGFMLDvhKRIHRLKQSLPKRKPGFMAKSYDPiap 198
Cdd:cd00009   11 REALELPPPKNLLLYGPPGTGKTTLARAIANELFRPGAPflYLNASDLLEG--LVVAELFGHFLVRLLFELAEKAKP--- 85

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 767942436 199 iaeeiseeaCLLCFDEFQ-VTDIADAMILKQL----FENLFKNGVVVVATSNRPPEGP 251
Cdd:cd00009   86 ---------GVLFIDEIDsLSRGAQNALLRVLetlnDLRIDRENVRVIGATNRPLLGD 134
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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