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Conserved domains on  [gi|1370464070|ref|XP_024305171|]
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regulator of nonsense transcripts 3A isoform X9 [Homo sapiens]

Protein Classification

regulator of nonsense transcripts 3A( domain architecture ID 10190789)

regulator of nonsense transcripts 3A (UPF3A) is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as a link between the EJC core and NMD machinery

Gene Ontology:  GO:0003723|GO:0042162|GO:0051028|GO:0000184
PubMed:  15853797|22278943|18515081
SCOP:  3000110

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RRM_like_Smg4_UPF3A cd12727
RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A ...
 66-152 1.21e-57

RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A (Upf3A); This subgroup corresponds to the RRM-like Smg4_UPF3 domain in Upf3A, also termed regulator of nonsense transcripts 3A, or nonsense mRNA reducing factor 3A, a human ortholog of yeast Upf3p and Caenorhabditis elegans SMG-4. It derives from gene UPF3A and is required for nonsense-mediated mRNA decay (NMD) in human. Upf3A is a nucleocytoplasmic shuttling protein that associates selectively with spliced beta-globin mRNA in vivo. Like other Upf3 proteins, Upf3A contains nuclear import and export signals, and a conserved Smg4_UPF3 domain with some similarity to an RNA recognition motif (RRM), indicating that it may be an RNA binding protein.


:

Pssm-ID: 410126  Cd Length: 87  Bit Score: 176.95  E-value: 1.21e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  66 ALSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYP 145
Cdd:cd12727     1 TLSKVVIRRLPPNLTKEQLEEQLRPLPAHDYFEFFAADQSLYPHLFSRAYINFKNPEDILLFRDRFDGYVFIDNKGQEYP 80


                  ....*..
gi 1370464070 146 AVVEFAP 152
Cdd:cd12727    81 AVVEFAP 87
 
Name Accession Description Interval E-value
RRM_like_Smg4_UPF3A cd12727
RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A ...
 66-152 1.21e-57

RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A (Upf3A); This subgroup corresponds to the RRM-like Smg4_UPF3 domain in Upf3A, also termed regulator of nonsense transcripts 3A, or nonsense mRNA reducing factor 3A, a human ortholog of yeast Upf3p and Caenorhabditis elegans SMG-4. It derives from gene UPF3A and is required for nonsense-mediated mRNA decay (NMD) in human. Upf3A is a nucleocytoplasmic shuttling protein that associates selectively with spliced beta-globin mRNA in vivo. Like other Upf3 proteins, Upf3A contains nuclear import and export signals, and a conserved Smg4_UPF3 domain with some similarity to an RNA recognition motif (RRM), indicating that it may be an RNA binding protein.


Pssm-ID: 410126  Cd Length: 87  Bit Score: 176.95  E-value: 1.21e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  66 ALSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYP 145
Cdd:cd12727     1 TLSKVVIRRLPPNLTKEQLEEQLRPLPAHDYFEFFAADQSLYPHLFSRAYINFKNPEDILLFRDRFDGYVFIDNKGQEYP 80


                  ....*..
gi 1370464070 146 AVVEFAP 152
Cdd:cd12727    81 AVVEFAP 87
Smg4_UPF3 pfam03467
Smg-4/UPF3 family; This family contains proteins that are involved in nonsense mediated mRNA ...
 68-208 8.41e-53

Smg-4/UPF3 family; This family contains proteins that are involved in nonsense mediated mRNA decay. A process that is triggered by premature stop codons in mRNA. The family includes Smg-4 and UPF3.


Pssm-ID: 460932 [Multi-domain]  Cd Length: 168  Bit Score: 167.46  E-value: 8.41e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  68 SKVVIRRLPPGLTKEQLEEQLRPLPA-HDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYPA 146
Cdd:pfam03467   4 TKVVIRRLPPNLTEEEFLEQLSPLPGeVDWFYFVPGKKSLKPPAYSRAYINFKDPEDLLQFADRFDGHVFVDSKGNEYPA 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370464070 147 VVEFAPFQKIAKKKLRKKDAKTGSIEDDPEYKKFLETYCVEEEKTSANPETLLGEMEAKTRE 208
Cdd:pfam03467  84 IVEFAPYQKVPKKKDKKKDAREGTIDKDPEYKEFLESLEKPEEDPESALIQEELEEEEEEKE 145
 
Name Accession Description Interval E-value
RRM_like_Smg4_UPF3A cd12727
RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A ...
 66-152 1.21e-57

RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog A (Upf3A); This subgroup corresponds to the RRM-like Smg4_UPF3 domain in Upf3A, also termed regulator of nonsense transcripts 3A, or nonsense mRNA reducing factor 3A, a human ortholog of yeast Upf3p and Caenorhabditis elegans SMG-4. It derives from gene UPF3A and is required for nonsense-mediated mRNA decay (NMD) in human. Upf3A is a nucleocytoplasmic shuttling protein that associates selectively with spliced beta-globin mRNA in vivo. Like other Upf3 proteins, Upf3A contains nuclear import and export signals, and a conserved Smg4_UPF3 domain with some similarity to an RNA recognition motif (RRM), indicating that it may be an RNA binding protein.


Pssm-ID: 410126  Cd Length: 87  Bit Score: 176.95  E-value: 1.21e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  66 ALSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYP 145
Cdd:cd12727     1 TLSKVVIRRLPPNLTKEQLEEQLRPLPAHDYFEFFAADQSLYPHLFSRAYINFKNPEDILLFRDRFDGYVFIDNKGQEYP 80


                  ....*..
gi 1370464070 146 AVVEFAP 152
Cdd:cd12727    81 AVVEFAP 87
RRM_like_Smg4_UPF3B cd12728
RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog B on ...
 67-154 1.74e-53

RNA recognition motif (RRM)-like Smg4_UPF3 domain in up-frameshift suppressor 3 homolog B on chromosome X (Upf3B); This subgroup corresponds to the RRM-like Smg4_UPF3 domain in Upf3B, also termed regulator of nonsense transcripts 3B, or nonsense mRNA reducing factor 3B, a human ortholog of yeast Upf3p and Caenorhabditis elegans SMG-4. It derives from X-linked gene UPF3B and is required for nonsense-mediated mRNA decay (NMD) in human. Upf3B is a nucleocytoplasmic shuttling protein that associates selectively with spliced beta-globin mRNA in vivo. Like other Upf3 proteins, Upf3B contains nuclear import and export signals, and a conserved Smg4_UPF3 domain with some similarity to an RNA recognition motif (RRM), indicating that it may be an RNA binding protein.


Pssm-ID: 410127  Cd Length: 89  Bit Score: 166.63  E-value: 1.74e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  67 LSKVVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYPA 146
Cdd:cd12728     1 LSKVVIRRLPPSLTKEQLEEHLQPLPEHDYFEFFSNDSSLYPHMFSRAYINFKNQEDIVLFRDRFDGYVFIDNRGQEYPA 80


                  ....*...
gi 1370464070 147 VVEFAPFQ 154
Cdd:cd12728    81 IVEFAPFQ 88
Smg4_UPF3 pfam03467
Smg-4/UPF3 family; This family contains proteins that are involved in nonsense mediated mRNA ...
 68-208 8.41e-53

Smg-4/UPF3 family; This family contains proteins that are involved in nonsense mediated mRNA decay. A process that is triggered by premature stop codons in mRNA. The family includes Smg-4 and UPF3.


Pssm-ID: 460932 [Multi-domain]  Cd Length: 168  Bit Score: 167.46  E-value: 8.41e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  68 SKVVIRRLPPGLTKEQLEEQLRPLPA-HDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEYPA 146
Cdd:pfam03467   4 TKVVIRRLPPNLTEEEFLEQLSPLPGeVDWFYFVPGKKSLKPPAYSRAYINFKDPEDLLQFADRFDGHVFVDSKGNEYPA 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1370464070 147 VVEFAPFQKIAKKKLRKKDAKTGSIEDDPEYKKFLETYCVEEEKTSANPETLLGEMEAKTRE 208
Cdd:pfam03467  84 IVEFAPYQKVPKKKDKKKDAREGTIDKDPEYKEFLESLEKPEEDPESALIQEELEEEEEEKE 145
RRM_like_Smg4_UPF3 cd12455
RNA recognition motif (RRM)-like Smg4_UPF3 domain in yeast up-frameshift suppressor 3 (Upf3p), ...
 69-152 1.28e-39

RNA recognition motif (RRM)-like Smg4_UPF3 domain in yeast up-frameshift suppressor 3 (Upf3p), Caenorhabditis elegans SMG-4, their human orthologs Upf3A and Upf3B, and similar proteins; This subfamily corresponds to the RRM-like Smg4_UPF3 domain found in yeast up-frameshift suppressor 3 (Upf3p), Caenorhabditis elegans SMG-4, their human orthologs Upf3A and Upf3B, and similar proteins. Upf3p, also termed nonsense-mediated mRNA decay protein 3, or Sua6p, a surveillance factor encoded by UPF3 gene from Saccharomyces cerevisiae. It is required for nonsense-mediated mRNA decay (NMD) in yeast. Upf3p is primarily cytoplasmic but accumulates inside the nucleus. Its nuclear import is mediated by the Srp1p (importin-alpha)/beta heterodimer while its nuclear export is mediated by a leucine-rich nuclear export sequence (NES-A), but not the Crm1p exportin. C. elegans SMG-4 is a nuclear shuttling protein that shuttles between the cytoplasm and nucleus through nuclear import and export signals similar to that of the yeast Upf3p. It is regulated by phosphorylation. Human orthologs of yeast Upf3p and C. elegans SMG-4 include Upf3A and Upf3B, which derive from two genes, UPF3A and X-linked UPF3B, respectively. Both, Upf3A (Up-frameshift suppressor 3 homolog A, also termed regulator of nonsense transcripts 3A, or nonsense mRNA reducing factor 3A) and Upf3B (Up-frameshift suppressor 3 homolog B on chromosome X, also termed regulator of nonsense transcripts 3B, or nonsense mRNA reducing factor 3B), are nucleocytoplasmic shuttling proteins. They associate selectively with spliced beta-globin mRNA in vivo, and tethering of any human Upf protein to the 3'UTR of beta-globin mRNA prevents NMD. The function of the Upf proteins in identifying and targeting nonsense mRNAs for rapid decay is conserved among eukaryotes. Besides, all Upf proteins in this family contain a conserved Smg4_UPF3 domain with some similarity to an RNA recognition motif (RRM), indicating that they may be RNA binding proteins.


Pssm-ID: 409889  Cd Length: 88  Bit Score: 131.23  E-value: 1.28e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  69 KVVIRRLPPGLTKEQLEEQLRPL--PAHDYFEFFAADLSL--YPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKGLEY 144
Cdd:cd12455     1 KVVIRRLPPSLTEEEFLEQIAPLikPTVDWFYFVGGKSSLdfKPPTYSRAYINFKNPEDLIEFRRKFDGYVFVDSKGNEY 80


                  ....*...
gi 1370464070 145 PAVVEFAP 152
Cdd:cd12455    81 PAIVEFAP 88
RRM3_MEI2_EAR1_like cd12277
RNA recognition motif 3 (RRM3) found in Mei2-like proteins and terminal EAR1-like proteins; ...
 70-154 2.46e-06

RNA recognition motif 3 (RRM3) found in Mei2-like proteins and terminal EAR1-like proteins; This subfamily corresponds to the RRM3 of Mei2-like proteins from plant and fungi, terminal EAR1-like proteins from plant, and other eukaryotic homologs. Mei2-like proteins represent an ancient eukaryotic RNA-binding proteins family whose corresponding Mei2-like genes appear to have arisen early in eukaryote evolution, been lost from some lineages such as Saccharomyces cerevisiae and metazoans, and diversified in the plant lineage. The plant Mei2-like genes may function in cell fate specification during development, rather than as stimulators of meiosis. In the fission yeast Schizosaccharomyces pombe, the Mei2 protein is an essential component of the switch from mitotic to meiotic growth. S. pombe Mei2 stimulates meiosis in the nucleus upon binding a specific non-coding RNA. The terminal EAR1-like protein 1 and 2 (TEL1 and TEL2) are mainly found in land plants. They may play a role in the regulation of leaf initiation. All members in this family are putative RNA-binding proteins carrying three RNA recognition motifs (RRMs), also termed RBDs (RNA binding domains) or RNPs (ribonucleoprotein domains). In addition to the RRMs, the terminal EAR1-like proteins also contain TEL characteristic motifs that allow sequence and putative functional discrimination between them and Mei2-like proteins.


Pssm-ID: 409719 [Multi-domain]  Cd Length: 86  Bit Score: 44.15  E-value: 2.46e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1370464070  70 VVIRRLPPGLTKEQLEEQLRPLPAHDYFEFFAA--DLSLYPHL-YsrAYINFRNPDDILLFRDRFDGYIfLDSKGLEYPA 146
Cdd:cd12277     1 VMLRNIPNKYTQEMLLQEIDEHGKGGAYDFFYLplDFKTKCNVgY--AFINFINPEAAERFYKAFNGKK-WKNFPSKKVC 77


                  ....*...
gi 1370464070 147 VVEFAPFQ 154
Cdd:cd12277    78 DISYARLQ 85
RRM_SF cd00590
RNA recognition motif (RRM) superfamily; RRM, also known as RBD (RNA binding domain) or RNP ...
 70-140 1.95e-03

RNA recognition motif (RRM) superfamily; RRM, also known as RBD (RNA binding domain) or RNP (ribonucleoprotein domain), is a highly abundant domain in eukaryotes found in proteins involved in post-transcriptional gene expression processes including mRNA and rRNA processing, RNA export, and RNA stability. This domain is 90 amino acids in length and consists of a four-stranded beta-sheet packed against two alpha-helices. RRM usually interacts with ssRNA, but is also known to interact with ssDNA as well as proteins. RRM binds a variable number of nucleotides, ranging from two to eight. The active site includes three aromatic side-chains located within the conserved RNP1 and RNP2 motifs of the domain. The RRM domain is found in a variety heterogeneous nuclear ribonucleoproteins (hnRNPs), proteins implicated in regulation of alternative splicing, and protein components of small nuclear ribonucleoproteins (snRNPs).


Pssm-ID: 409669 [Multi-domain]  Cd Length: 72  Bit Score: 35.72  E-value: 1.95e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1370464070  70 VVIRRLPPGLTKEQLEEQLRPlpahdYFEFFAADLSLYPHLYSR--AYINFRNPDDILLFRDRFDGYIFLDSK 140
Cdd:cd00590     1 LFVGNLPPDTTEEDLRELFSK-----FGEVVSVRIVRDRDGKSKgfAFVEFESPEDAEKALEALNGTELGGRP 68
RRM_1 pfam00076
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain); The RRM motif is probably diagnostic ...
 70-140 4.89e-03

RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain); The RRM motif is probably diagnostic of an RNA binding protein. RRMs are found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs. The motif also appears in a few single stranded DNA binding proteins. The RRM structure consists of four strands and two helices arranged in an alpha/beta sandwich, with a third helix present during RNA binding in some cases The C-terminal beta strand (4th strand) and final helix are hard to align and have been omitted in the SEED alignment The LA proteins have an N terminal rrm which is included in the seed. There is a second region towards the C terminus that has some features characteriztic of a rrm but does not appear to have the important structural core of a rrm. The LA proteins are one of the main autoantigens in Systemic lupus erythematosus (SLE), an autoimmune disease.


Pssm-ID: 425453 [Multi-domain]  Cd Length: 70  Bit Score: 34.52  E-value: 4.89e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1370464070  70 VVIRRLPPGLTKEQLEeqlrplpahDYFEFF----AADLSLYPHLYSR--AYINFRNPDDILLFRDRFDGYIFLDSK 140
Cdd:pfam00076   1 LFVGNLPPDTTEEDLK---------DLFSKFgpikSIRLVRDETGRSKgfAFVEFEDEEDAEKAIEALNGKELGGRE 68
HDAC3 cd10005
Histone deacetylase 3 (HDAC3); HDAC3 is a Zn-dependent class I histone deacetylase that ...
 79-114 5.55e-03

Histone deacetylase 3 (HDAC3); HDAC3 is a Zn-dependent class I histone deacetylase that catalyzes hydrolysis of N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone (EC 3.5.1.98). Histone acetylation/deacetylation process is important for mediation of transcriptional regulation of many genes. In order to target specific chromatin regions, HDAC3 can interact with DNA-binding proteins (transcriptional factors) either directly or after forming complexes with a number of other proteins, as observed for the SMPT/N-CoR complex which recruits human HDAC3 to specific chromatin loci and activates deacetylation. Human HDAC3 is also involved in deacetylation of non-histone substrates such as RelA, SPY and p53 factors. This protein can also down-regulate p53 function and subsequently modulate cell growth and apoptosis. This gene is therefore regarded as a potential tumor suppressor gene. HDAC3 plays a role in various physiological processes, including subcellular protein localization, cell cycle progression, cell differentiation, apoptosis and survival. HDAC3 has been found to be overexpressed in some tumors including leukemia, lung carcinoma, colon cancer and maxillary carcinoma. Thus, inhibitors precisely targeting HDAC3 (in some cases together with retinoic acid or hyperthermia) could be a therapeutic drug option.


Pssm-ID: 212529  Cd Length: 381  Bit Score: 36.99  E-value: 5.55e-03
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 1370464070  79 LTKEQLEEQLrplPAHDYFEFFAADLSLYPHLYSRA 114
Cdd:cd10005   312 LVDEEISNEL---PYNEYFEYFAPDFTLHPDVSTRI 344
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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