NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1958667260|ref|XP_038944849|]
View 

run domain Beclin-1-interacting and cysteine-rich domain-containing protein isoform X1 [Rattus norvegicus]

Protein Classification

run domain Beclin-1-interacting and cysteine-rich domain-containing protein( domain architecture ID 17730331)

run domain Beclin-1-interacting and cysteine-rich domain-containing protein (Rubicon) is part of a Beclin-1-Vps34-containing autophagy complex; it negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process

CATH:  1.20.58.900
Gene Symbol:  RUBCN
Gene Ontology:  GO:1901981|GO:0006914|GO:0006897
PubMed:  11166556
SCOP:  4001476

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
zf-RING_9 pfam13901
Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members ...
 727-927 2.52e-101

Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members also carry a pleckstrin-homology domain, pfam00169


:

Pssm-ID: 464030  Cd Length: 205  Bit Score: 314.94  E-value: 2.52e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 727 RYCEYLGKYFCQCCHENAQMTVPSRILRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQL 806
Cdd:pfam13901   1 RLCDYTGKYYCSGCHWNDTSVIPARILHNWDFKKYPVSKFAKQLLDSIYSQPLLNLSDLNPSLYSKVKELAKVRELREQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 807 YHMKNMFKTCRLA--EELLDSFDVV-PGHLTEDLHLYSLNDLTAAKKGELGPRLAELTRAGAAHVERCMLCQAKGFICEF 883
Cdd:pfam13901  81 KLLKDYLKTCRFAaeEELLKLFRLRpRHHLLEDSHLYSLQDLVDIKNGSLLPFLEELVQFAEKHVTNCELCQGKGFICEL 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1958667260 884 CQNeDDVIFPFELHKCRTCEECRACYHKTCFKSGS--CPRCERLQA 927
Cdd:pfam13901 161 CNS-DDIIFPFDIDTTSRCEKCKAVFHKSCFRSGAspCPKCERLQK 205
RUN_RUBCN cd17686
RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing ...
 34-190 5.38e-69

RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (RUBCN) and similar proteins; RUBCN, also called rubicon, or beclin-1 associated RUN domain containing protein (Baron), is part of a Beclin-1-Vps34-containing autophagy complex. It negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. It is also an important negative regulator of the innate immune response, enhances viral replication and may play a role in viral immune evasion. This model contains the RUN domain of RUBCN.


:

Pssm-ID: 439048  Cd Length: 151  Bit Score: 226.38  E-value: 5.38e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  34 TTVEGLVSANCPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWLSPHSALHVEKFISLHESGQSDT 113
Cdd:cd17686     1 STVEGLLLSRSSNVWSTYGGLQRLCRAVENILQHGLKEFQGLNKEIDDWEFVQGLRWLQPTLAPSIEQQSRSSPSESEVS 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958667260 114 nsvsehTVAELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAVEQNNPRLLAQIDASM 190
Cdd:cd17686    81 ------DKGRLWLRQSLQQHCLSSQLQWLVSDKELLRKYYEDEAFLRQEGYATALLICLTAVELNQPSLLAQIDPSL 151
 
Name Accession Description Interval E-value
zf-RING_9 pfam13901
Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members ...
 727-927 2.52e-101

Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members also carry a pleckstrin-homology domain, pfam00169


Pssm-ID: 464030  Cd Length: 205  Bit Score: 314.94  E-value: 2.52e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 727 RYCEYLGKYFCQCCHENAQMTVPSRILRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQL 806
Cdd:pfam13901   1 RLCDYTGKYYCSGCHWNDTSVIPARILHNWDFKKYPVSKFAKQLLDSIYSQPLLNLSDLNPSLYSKVKELAKVRELREQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 807 YHMKNMFKTCRLA--EELLDSFDVV-PGHLTEDLHLYSLNDLTAAKKGELGPRLAELTRAGAAHVERCMLCQAKGFICEF 883
Cdd:pfam13901  81 KLLKDYLKTCRFAaeEELLKLFRLRpRHHLLEDSHLYSLQDLVDIKNGSLLPFLEELVQFAEKHVTNCELCQGKGFICEL 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1958667260 884 CQNeDDVIFPFELHKCRTCEECRACYHKTCFKSGS--CPRCERLQA 927
Cdd:pfam13901 161 CNS-DDIIFPFDIDTTSRCEKCKAVFHKSCFRSGAspCPKCERLQK 205
RUN_RUBCN cd17686
RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing ...
 34-190 5.38e-69

RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (RUBCN) and similar proteins; RUBCN, also called rubicon, or beclin-1 associated RUN domain containing protein (Baron), is part of a Beclin-1-Vps34-containing autophagy complex. It negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. It is also an important negative regulator of the innate immune response, enhances viral replication and may play a role in viral immune evasion. This model contains the RUN domain of RUBCN.


Pssm-ID: 439048  Cd Length: 151  Bit Score: 226.38  E-value: 5.38e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  34 TTVEGLVSANCPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWLSPHSALHVEKFISLHESGQSDT 113
Cdd:cd17686     1 STVEGLLLSRSSNVWSTYGGLQRLCRAVENILQHGLKEFQGLNKEIDDWEFVQGLRWLQPTLAPSIEQQSRSSPSESEVS 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958667260 114 nsvsehTVAELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAVEQNNPRLLAQIDASM 190
Cdd:cd17686    81 ------DKGRLWLRQSLQQHCLSSQLQWLVSDKELLRKYYEDEAFLRQEGYATALLICLTAVELNQPSLLAQIDPSL 151
RUN smart00593
domain involved in Ras-like GTPase signaling;
122-182 3.54e-11

domain involved in Ras-like GTPase signaling;


Pssm-ID: 214736 [Multi-domain]  Cd Length: 64  Bit Score: 59.55  E-value: 3.54e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958667260  122 AELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVT---AMLQCLEAVEQNNPRL 182
Cdd:smart00593   1 FRAWIRLALNEKLLSSWLNLLLSDEELLSKYYEPWAFLRDPEEGEqllGLLVGLSALDFNLPVD 64
 
Name Accession Description Interval E-value
zf-RING_9 pfam13901
Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members ...
 727-927 2.52e-101

Putative zinc-RING and/or ribbon; This is a family of cysteine-rich proteins. Many members also carry a pleckstrin-homology domain, pfam00169


Pssm-ID: 464030  Cd Length: 205  Bit Score: 314.94  E-value: 2.52e-101
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 727 RYCEYLGKYFCQCCHENAQMTVPSRILRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQL 806
Cdd:pfam13901   1 RLCDYTGKYYCSGCHWNDTSVIPARILHNWDFKKYPVSKFAKQLLDSIYSQPLLNLSDLNPSLYSKVKELAKVRELREQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260 807 YHMKNMFKTCRLA--EELLDSFDVV-PGHLTEDLHLYSLNDLTAAKKGELGPRLAELTRAGAAHVERCMLCQAKGFICEF 883
Cdd:pfam13901  81 KLLKDYLKTCRFAaeEELLKLFRLRpRHHLLEDSHLYSLQDLVDIKNGSLLPFLEELVQFAEKHVTNCELCQGKGFICEL 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1958667260 884 CQNeDDVIFPFELHKCRTCEECRACYHKTCFKSGS--CPRCERLQA 927
Cdd:pfam13901 161 CNS-DDIIFPFDIDTTSRCEKCKAVFHKSCFRSGAspCPKCERLQK 205
RUN_RUBCN cd17686
RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing ...
 34-190 5.38e-69

RUN domain found in Run domain Beclin-1-interacting and cysteine-rich domain-containing protein (RUBCN) and similar proteins; RUBCN, also called rubicon, or beclin-1 associated RUN domain containing protein (Baron), is part of a Beclin-1-Vps34-containing autophagy complex. It negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. It is also an important negative regulator of the innate immune response, enhances viral replication and may play a role in viral immune evasion. This model contains the RUN domain of RUBCN.


Pssm-ID: 439048  Cd Length: 151  Bit Score: 226.38  E-value: 5.38e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  34 TTVEGLVSANCPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWLSPHSALHVEKFISLHESGQSDT 113
Cdd:cd17686     1 STVEGLLLSRSSNVWSTYGGLQRLCRAVENILQHGLKEFQGLNKEIDDWEFVQGLRWLQPTLAPSIEQQSRSSPSESEVS 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1958667260 114 nsvsehTVAELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAVEQNNPRLLAQIDASM 190
Cdd:cd17686    81 ------DKGRLWLRQSLQQHCLSSQLQWLVSDKELLRKYYEDEAFLRQEGYATALLICLTAVELNQPSLLAQIDPSL 151
RUN cd17671
RUN domain; RUN domain, named after RPIP8 (Rap2 interacting protein 8), UNC-14 and NESCA (new ...
 37-177 5.88e-19

RUN domain; RUN domain, named after RPIP8 (Rap2 interacting protein 8), UNC-14 and NESCA (new molecule containing SH3 at the carboxyl-terminus), is a less conserved protein motif that comprises six conserved regions, which in some proteins have considerable insertions between them. The domain core is thought to take up a predominantly alpha fold, with basic amino acids in regions A and D possibly playing a functional role in interactions with Ras GTPases. RUN domains are often found in proteins linked particularly to the functions of GTPases in the Rap and Rab families, suggesting the RUN domain may be involved in Rab-mediated membrane trafficking, possibly as a Rab-binding site. RUN domain-containing proteins could hence play important roles in multiple Ras-like GTPase signalling pathways.


Pssm-ID: 439038  Cd Length: 154  Bit Score: 84.78  E-value: 5.88e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  37 EGLVSANCPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWL--SPHSALHVEKFISLHesgqsdtN 114
Cdd:cd17671    14 GEADDSAALTLTDDDPVVGRLCAALEAILSHGLKPKRFGGGKVSFWDFLEALEKLlpAPSLKQAIRDINSLS-------N 86

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1958667260 115 SVSEHTVAELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAVEQ 177
Cdd:cd17671    87 VKTDDGRGRAWIRLALNEKSLESYLAALLSDQSLLRKYYEPWALLRDPEEAELFLSLLVGLSS 149
RUN smart00593
domain involved in Ras-like GTPase signaling;
122-182 3.54e-11

domain involved in Ras-like GTPase signaling;


Pssm-ID: 214736 [Multi-domain]  Cd Length: 64  Bit Score: 59.55  E-value: 3.54e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1958667260  122 AELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVT---AMLQCLEAVEQNNPRL 182
Cdd:smart00593   1 FRAWIRLALNEKLLSSWLNLLLSDEELLSKYYEPWAFLRDPEEGEqllGLLVGLSALDFNLPVD 64
RUN_PLEKHM2 cd17680
RUN domain found in pleckstrin homology domain-containing family M member 2 (PLEKHM2) and ...
 54-176 9.29e-06

RUN domain found in pleckstrin homology domain-containing family M member 2 (PLEKHM2) and similar proteins; PLEKHM2, also called PH domain-containing family M member 2, or Salmonella-induced filaments A (SifA) and Kinesin-Interacting Protein (SKIP), is the lysosome, melanosome and lytic granule cargo adaptor that controls lysosome positioning using a composite kinesin-1 heavy and light chain-binding domain. In addition to kinesin-1, it also interacts with several Rabs to affect endosomal trafficking. This model represents the RUN domain of PLEKHM2.


Pssm-ID: 439042  Cd Length: 145  Bit Score: 46.47  E-value: 9.29e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  54 LERLCRDMQNILYHGLIHDQVCcrqadYWQFVKDIRWLSphsalhVEKFISLHESGQSDTN-SVSehtvaelWLQHSLQC 132
Cdd:cd17680    34 LQRLCEALDHALLHGLRRGNRG-----YWPFVKEFTHKE------TIKQIENLPNVTTDLGrGRA-------WLYLALNE 95

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1958667260 133 HCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAVE 176
Cdd:cd17680    96 GSLESYLRSFLENRKLVKKFYHKHALLRDSQRLELLLTLLSGLE 139
RUN_RUFY4_like cd17682
RUN domain found in RUN and FYVE domain-containing protein 4 (RUFY4), FYVE and coiled-coil ...
 31-175 4.30e-03

RUN domain found in RUN and FYVE domain-containing protein 4 (RUFY4), FYVE and coiled-coil domain-containing protein 1 (FYCO1), and similar proteins; The family includes RUFY4 and FYCO1. RUFY4 acts as a positive regulator that enhances autophagy and lysosome tethering in response to Interleukin-4. It is expressed in a cell-specific manner or under specific immunological conditions associated with IL4 expression such as allergic asthma. FYCO1, also called zinc finger FYVE domain-containing protein 7 (ZFYVE7), is a multidomain autophagy adaptor protein that interacts with kinesin motor proteins and with the autophagosomal membrane components microtubule-associated protein 1 light chain 3 (LC3), Rab7, and phosphatidylinositol 3-phosphate (PI3P), to mediate microtubule plus-end-directed autophagosome transport. Both RUFY4 and FYCO1 contain an N-terminal RUN domain and a C-terminal FYVE domain with two coiled-coil domains in-between; this model represents the RUN domain.


Pssm-ID: 439044  Cd Length: 150  Bit Score: 38.75  E-value: 4.30e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1958667260  31 NLKTTVEGLVSANCPNVWSKYGGLERLCRDMQNILYHGLI-HDQVCCRQADYWQFVKDI---RWLSPHSALHVEKFIslh 106
Cdd:cd17682     1 DLKGCVLDLKSEFGEITDPDNPYLRPFCETLEKILRKGLKeKVSLGGRRKDYWDWLEELlkkLNKIPKSLSDAVKFV--- 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1958667260 107 esgQSDTNSVSEHTVAELWLQHSLQCHCLSAQLRPLLGDRQYMRKFYTETAFLLSGAHVTAMLQCLEAV 175
Cdd:cd17682    78 ---KSCKKVKTNQGRGRLFIRYALNKKCLHDPVQQLVKNPKLLSDYYSPDSILGNEILSEILLSLLYQL 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH