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IDs: 585011 [UID] 2689978 [GenBank] 2693128 [RefSeq]
Background: The Priapulus caudatus DNA for Illumina shotgun sequencing is derived from a single male. This worm was collected by the lab of Andreas Hejnol, Sars International Centre for Marine Molecular Biology, from mud at 60-100m depth in Gulmarsfjord, ... Sweden from a wild population (Approx. Latitude 58 degrees 16.2 minutes North. Longitude = 11 degrees, 27.2 minutes East). The Priapulus multiple DNA samples for the La Roche 454 data was obtained by Ralf Janssen and Graham E Budd, Uppsala University, and was collected at the same approximate location as above. Total input sequence coverage of Illumina instrument fragment reads was 68X, while the final assembled coverage (fragments, 3, and 8kbs) was approximately 100X using a genome size estimate of 300Mb. The combined sequence reads were assembled using the ALLPATHS-LG software (Gnerre et al 2011). The ALLPATHS assembly was then merged with a low coverage (28.5X) 454 Roche Newbler (Knight) assembly. The single individual ALLPATHS-LG assembly served as the reference and represents the majority of the merged assembly. This initial merged draft assembly was referred to as Priapulus caudatus 4.0.1. Additionally, 13X of PacBio long reads were sequenced and used to gap fill using PBJelly (English et al). This hybrid 5.0.1 version has been screened and cleaned of contaminating contigs. The 5.0.1 assembly is made up of a total of 59,734 scaffolds with an N50 scaffold length of 197,174bp (N50 contig length is 10,616bp). The assembly spans 514Mb including gaps, and singleton scaffolds. This work was supported by NIH large grant HG003079 to Richard K. Wilson, The Genome Institute at Washington University School of Medicine. For questions regarding this Priapulus caudatus 5.0.1 assembly please contact Wesley Warren, Washington University School of Medicine (pminx@genome.wustl.edu). Funding for the sequence characterization of the Priapulus caudatus genome was provided by the National Institutes of Health (NIH). DNA samples can be obtained from: Dr Andreas Hejnol Sars International Centre for Marine Molecular Biology Thormohlensgate 55 NO-5008 Bergen NORWAY Sequence and Assembly Credits: Source DNA - Dr Andreas Hejnol, Sars International Centre for Marine Molecular Biology, Bergen NORWAY Genome Sequence - The McDonnell Genome Institute, Washington University School of Medicine Sequence Assembly - The McDonnell Genome Institute, Washington University School of Medicine It is requested that users of this Priapulus caudatus 5.0 sequence assembly acknowledge Richard K. Wilson, Director, and the McDonnell Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. Assembly Statistics (Supercontig = Scaffold) *** Contiguity: Contig *** Total contig number: 121508 Total contig bases: 436602170 bp Average contig length: 3593 bp Maximum contig length: 150106 bp N50 contig length: 10616 bp N50 contig number: 11805 *** Contiguity: Supercontig *** Total supercontig number: 59731 Average supercontig length: 7309 bp Maximum supercontig length: 1650746 bp N50 supercontig length: 197174 bp N50 supercontig number: 53 *** Scafold Distribution *** Scaffolds > 1M: 18 Scaffold 250K--1M: 377 Scaffold 100K--250K: 648 Scaffold 10--100K: 3001 Scaffold 5--10K: 1666 Scaffold 2--5K: 3132 Scaffold 0--2K: 50889 more
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