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Background:
 The Limulus polyphemus DNA used for sequencing was derived from a single male animal (carapace length approximately 16 cm) collected January 2008 in Woods Hole, MA. Muscle tissue was extracted from the limbs, and genomic DNA was prepared ... in the lab of Nipam Patel by Meredith Protas.
 Total sequence coverage of Roche 454 reads was approximately 18C (fragments, 3 and 8kbs) using a genome size estimate of 1.5Gb. The combined sequence reads were assembled using the Newbler software (Roche). This draft assembly was referred to as Limulus polyphemus 2.1.1. This version has been gap filled, error corrected with 
12X Illumina reads, and cleaned of contaminating contigs. The assembly is made up of a total of 286,840 scaffolds with an N50 scaffold length of 238kb (N50 contig length was 11.4kb). The assembly spans 1.7Gb.

 This work was funded by NIH grant HG003079 to RKW, The Genome Institute at Washington University School of Medicine.
 For questions regarding this Limulus polyphemus 2.1.1 assembly please contact Patrick Minx, Washington University School of Medicine (pminx@genome.wustl.edu). Funding for the sequence characterization of the Limulus polyphemus genome was provided by the Office of Director, National Institutes of Health (NIH). ***************************************************************************
 Sequence and Assembly Credits:
 Sequencing DNA source credit: Nipam Patel and Meredith Protas
 DNA source can be obtained from: Nipam Patel Dept. of Molecular and Cell Biology 519A LSA 3200 UC Berkeley Berkeley, CA 94720-3200
 Genome Sequence - The Genome Institute, Washington University School of Medicine
 Sequence Assembly - The Genome Institute, Washington University School of Medicine
 Citation upon use of this assembly in a manuscript:
 It is requested that users of this Limulus polyphemus 2.1.1 sequence assembly acknowledge Richard K. Wilson and The Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. 
 Assembly Stats
 *** Contiguity: Contig *** Total contig number: 469557 Total contig bases: 1705828287 bp Average contig length: 3633 bp Maximum contig length: 133547 bp N50 contig length: 11441 bp N50 contig number: 41760
 *** Contiguity: Supercontig *** Total supercontig number: 286840 Average supercontig length: 5947 bp Maximum supercontig length: 4914972 bp N50 supercontig length: 238003 bp N50 supercontig number: 1693
 *** Scaffold Distribution *** Scaffolds > 1M: 128 Scaffold 250K--1M: 1451 Scaffold 100K--250K: 2309 Scaffold 10--100K: 8850 Scaffold 5--10K: 3013 Scaffold 2--5K: 8455 Scaffold 0--2K: 262634  more

Global statistics