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The rhesus macaque (Macaca mulatta) DNA was derived from a fibroblast cell line (Coriell sample id: AG07107, MMU) of a single Indian origin female. The cell line was not transformed and taken at two years of age. Sequences were ... generated on the Pacific Biosciences RSII instrument (V2 chemistry) to approximately 66x genome coverage based on a genome size estimate of 3 Gb. All SMRT sequences were assembled with the HGAP4 algorithm (SMRT Link v5.0.1.9585) then error corrected using the Arrow error-correction module. Additional polishing of the assembly for residual indels was done by aligning 50x coverage of Illumina data and then running the Pilon algorithm. Finally, any residual indels were detected and corrected using a custom pipeline in the lab of Dr. Evan Eichler. All detected chimeric contigs were broken using a BioNano map as guidance, as well as the initial scaffolding process. Final chromosomal level scaffolds/contig files were generated by alignment to HiC data (Richard Green) and to the Mmul 8.0.1 reference. 
 Of the total sequence in scaffolds, 2,939,559,484 bp, in the assembled genome (Mmul_9.0), the assembly total contig number and N50 contig lengths are 3177 and 46.6 Mb (N=22), respectively. The total scaffold number and N50 scaffold lengths are 2975 and 82.3 Mb (N=14), respectively.
 Chromosome Y (CM003438) was added to Mmul_9.0, creating Mmul_10.
 For questions regarding the use of the Macaca mulatta Mmul_9.0 or Mmul_10 assembly please contact Dr. Wes Warren, Bond Life Sciences Center, University of Missouri, Columbia, MO; Dr. Evan Eichler, University of Washington, Seattle, WA or Tina Lindsay, McDonnell Genome Institute, Washington University, St Louis, MO. 
 Data use: The Macaca mulatta Mmul_9.0 and Mmul_10 assembly sequences are made available to the community by the McDonnell Genome Institute, Washington University, St Louis, MO with the following understanding: Users are free to analyze these data if the providers of this data are properly cited, any whole genome analyses should follow guidelines outlined in the Toronto agreement on the use of this assembly.
 Macaca mulatta Sequence and Assembly Credits: Sequencing Funds - This work was supported by the NHGRI grant No. 5U24HG00908103, HIGH QUALITY HUMAN AND NON-HUMAN PRIMATE GENOME ASSEMBLIES, at the McDonnell Genome Institute, Washington University School of Medicine DNA source - Coriell sample id: AG07107, MMU Genome Sequencing - The McDonnell Genome Institute, Washington University School of Medicine. Sequence Assembly - The McDonnell Genome Institute, Washington University School of Medicine. Sequence assembly curation - The McDonnell Genome Institute, Washington University School of Medicine; The University of Washington, Seattle, WA.  more

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