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Grants:#NIH Grant 1DP2OD028806-01#NSF EDGE award 1923372#(both to Dr. Nicolas Rohner)

The Astyanax mexicanus DNA sample was obtained from a female fish that was a reared at the Stowers Institute aquatic facility. High molecular weight DNA was used as input to ... generate long-reads using circular consensus sequence (CCS) base calling output from the Sequel II instrument (Pacific Biosciences) according to the manufacturer's protocols. Total CCS coverage was approx. 38x using a genome size estimate of 1.4Gb. All quality filtered sequence reads were assembled with HiFasm using default parameter settings and redundant contigs removed using purge_haplotigs. Assembled contigs were scaffolded using HiC sequence data from a sibling individual using Salsa 2 and JuiceBox. Chromosomes were numbered by synteny to previous assembled chromosomes of Asytanax mexicanus. This version of the surface fish assembly will replace the prior version GCA_000372685.2.

It is requested that users of this Astyanax mexicanus 1.0 sequence assembly acknowledge the Astyanax mexicanus genomes consortium in any publications that result from use of this sequence assembly prior to the publication of this resource. Any publications that propose to generate whole genome analyses using this resource should contact Dr. Nicolas Rohner (nro@stowers.org) or Dr. Wes Warren (warrenwc@missouri.edu) for the use of pre-publication data in accord with the Ft. Lauderdale and Toronto genomic data use agreements.  more

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